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ebfp2 cdna  (Addgene inc)


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    Structured Review

    Addgene inc ebfp2 cdna
    Ebfp2 Cdna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ebfp2+sequence/eBFP2+(eBFP2)_CD+(Plasmid+%2366034)/pmc05732323-359-8-13
    Average 93 stars, based on 31 article reviews
    ebfp2 cdna - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: Engineered Feedback Employing Natural Hypoxia-Responsive Factors Enhances Synthetic Hypoxia Biosensors
    Article Snippet: .. The minimal promoter SV40_min, the EF1α and TRE3GV promoters, the genes encoding the stable HIF1α mutant, TetON3G, BlastR, EBFP2, and EYFP; and the CHS4 insulator were sourced from plasmids from Addgene and Clontech as described in Supplementary Table 11 ( , – ). .. The genes for mTagBFP2 , mNeonGreen , mRuby3 , miRFP670 ( , ), and miRFP720 ( ) were synthesized as codon-optimized Gene Strings by Thermo Fisher.

    Article Title: Engineered Feedback Employing Natural Hypoxia-Responsive Factors Enhances Synthetic Hypoxia Biosensors.
    Article Snippet: .. A previously characterized HBS20 was synthesized as overlapping oligonucleotides, as were the minimal promoters YB_TATA and CMV_min.31 The minimal promoter SV40_min, the EF1α and TRE3GV promoters, the genes encoding the stable HIF1α mutant, TetON3G, BlastR, EBFP2, and EYFP; and the CHS4 insulator were sourced from plasmids from Addgene and Clontech as described in Supporting Table 11.31,42,53−56 The genes for mTagBFP2,57 mNeonGreen,58 mRuby3,59 miRFP670,60,61 and miRFP72062 were synthesized as codon-optimized Gene Strings by Thermo Fisher. .. A previously characterized HBS20 was synthesized as overlapping oligonucleotides, as were the minimal promoters YB_TATA and CMV_min.31 The minimal promoter SV40_min, the EF1α and TRE3GV promoters, the genes encoding the stable HIF1α mutant, TetON3G, BlastR, EBFP2, and EYFP; and the CHS4 insulator were sourced from plasmids from Addgene and Clontech as described in Supporting Table 11.31,42,53−56 The genes for mTagBFP2,57 mNeonGreen,58 mRuby3,59 miRFP670,60,61 and miRFP72062 were synthesized as codon-optimized Gene Strings by Thermo Fisher.

    Synthesized:

    Article Title: Engineered Feedback Employing Natural Hypoxia-Responsive Factors Enhances Synthetic Hypoxia Biosensors.
    Article Snippet: .. A previously characterized HBS20 was synthesized as overlapping oligonucleotides, as were the minimal promoters YB_TATA and CMV_min.31 The minimal promoter SV40_min, the EF1α and TRE3GV promoters, the genes encoding the stable HIF1α mutant, TetON3G, BlastR, EBFP2, and EYFP; and the CHS4 insulator were sourced from plasmids from Addgene and Clontech as described in Supporting Table 11.31,42,53−56 The genes for mTagBFP2,57 mNeonGreen,58 mRuby3,59 miRFP670,60,61 and miRFP72062 were synthesized as codon-optimized Gene Strings by Thermo Fisher. .. A previously characterized HBS20 was synthesized as overlapping oligonucleotides, as were the minimal promoters YB_TATA and CMV_min.31 The minimal promoter SV40_min, the EF1α and TRE3GV promoters, the genes encoding the stable HIF1α mutant, TetON3G, BlastR, EBFP2, and EYFP; and the CHS4 insulator were sourced from plasmids from Addgene and Clontech as described in Supporting Table 11.31,42,53−56 The genes for mTagBFP2,57 mNeonGreen,58 mRuby3,59 miRFP670,60,61 and miRFP72062 were synthesized as codon-optimized Gene Strings by Thermo Fisher.

    Plasmid Preparation:

    Article Title: Fn14 is an activity-dependent, Bmal1-regulated cytokine receptor that induces rod-like microglia and restricts neuronal activity in vivo.
    Article Snippet: .. The hSyn-eBFP2 plasmid was constructed by PCR amplification and elongation of the eBFP2 sequence from Addgene plasmid 66034 using the following PCR primers: (capitalized sequences anneal to eBFP2 whereas noncapitalized sequences are elongation sequences): eBFP2 Elongation Forward: gaaggtaccggatccgccacGATGGTGAGCAAGGGCGAG; eBFP2 ElongationReverse: tatcgataagcttgatatcgCAGCGAGTCAGTGAGCGAG. .. After elongation and amplification, the eBFP2 insert was assembled into Addgene plasmid 50465 after EcoRI-HF (NEB:R3101s) and BamHI-HF (NEB:R3136s) restriction digest using a Gibson reaction (NEB: E2621S).

    Article Title: Induction of post-meiotic DNA double-strand breaks by the Pnu1 endonuclease in <i>Schizosaccharomyces pombe</i>
    Article Snippet: 1 Title Page Title: Induction of post-meiotic DNA double-strand breaks by the Pnu1 endonuclease in

    Construct:

    Article Title: Fn14 is an activity-dependent, Bmal1-regulated cytokine receptor that induces rod-like microglia and restricts neuronal activity in vivo.
    Article Snippet: .. The hSyn-eBFP2 plasmid was constructed by PCR amplification and elongation of the eBFP2 sequence from Addgene plasmid 66034 using the following PCR primers: (capitalized sequences anneal to eBFP2 whereas noncapitalized sequences are elongation sequences): eBFP2 Elongation Forward: gaaggtaccggatccgccacGATGGTGAGCAAGGGCGAG; eBFP2 ElongationReverse: tatcgataagcttgatatcgCAGCGAGTCAGTGAGCGAG. .. After elongation and amplification, the eBFP2 insert was assembled into Addgene plasmid 50465 after EcoRI-HF (NEB:R3101s) and BamHI-HF (NEB:R3136s) restriction digest using a Gibson reaction (NEB: E2621S).

    Polymerase Chain Reaction:

    Article Title: Fn14 is an activity-dependent, Bmal1-regulated cytokine receptor that induces rod-like microglia and restricts neuronal activity in vivo.
    Article Snippet: .. The hSyn-eBFP2 plasmid was constructed by PCR amplification and elongation of the eBFP2 sequence from Addgene plasmid 66034 using the following PCR primers: (capitalized sequences anneal to eBFP2 whereas noncapitalized sequences are elongation sequences): eBFP2 Elongation Forward: gaaggtaccggatccgccacGATGGTGAGCAAGGGCGAG; eBFP2 ElongationReverse: tatcgataagcttgatatcgCAGCGAGTCAGTGAGCGAG. .. After elongation and amplification, the eBFP2 insert was assembled into Addgene plasmid 50465 after EcoRI-HF (NEB:R3101s) and BamHI-HF (NEB:R3136s) restriction digest using a Gibson reaction (NEB: E2621S).

    Article Title: Induction of post-meiotic DNA double-strand breaks by the Pnu1 endonuclease in <i>Schizosaccharomyces pombe</i>
    Article Snippet: 1 Title Page Title: Induction of post-meiotic DNA double-strand breaks by the Pnu1 endonuclease in

    Amplification:

    Article Title: Fn14 is an activity-dependent, Bmal1-regulated cytokine receptor that induces rod-like microglia and restricts neuronal activity in vivo.
    Article Snippet: .. The hSyn-eBFP2 plasmid was constructed by PCR amplification and elongation of the eBFP2 sequence from Addgene plasmid 66034 using the following PCR primers: (capitalized sequences anneal to eBFP2 whereas noncapitalized sequences are elongation sequences): eBFP2 Elongation Forward: gaaggtaccggatccgccacGATGGTGAGCAAGGGCGAG; eBFP2 ElongationReverse: tatcgataagcttgatatcgCAGCGAGTCAGTGAGCGAG. .. After elongation and amplification, the eBFP2 insert was assembled into Addgene plasmid 50465 after EcoRI-HF (NEB:R3101s) and BamHI-HF (NEB:R3136s) restriction digest using a Gibson reaction (NEB: E2621S).

    Article Title: Induction of post-meiotic DNA double-strand breaks by the Pnu1 endonuclease in <i>Schizosaccharomyces pombe</i>
    Article Snippet: 1 Title Page Title: Induction of post-meiotic DNA double-strand breaks by the Pnu1 endonuclease in

    Sequencing:

    Article Title: Fn14 is an activity-dependent, Bmal1-regulated cytokine receptor that induces rod-like microglia and restricts neuronal activity in vivo.
    Article Snippet: .. The hSyn-eBFP2 plasmid was constructed by PCR amplification and elongation of the eBFP2 sequence from Addgene plasmid 66034 using the following PCR primers: (capitalized sequences anneal to eBFP2 whereas noncapitalized sequences are elongation sequences): eBFP2 Elongation Forward: gaaggtaccggatccgccacGATGGTGAGCAAGGGCGAG; eBFP2 ElongationReverse: tatcgataagcttgatatcgCAGCGAGTCAGTGAGCGAG. .. After elongation and amplification, the eBFP2 insert was assembled into Addgene plasmid 50465 after EcoRI-HF (NEB:R3101s) and BamHI-HF (NEB:R3136s) restriction digest using a Gibson reaction (NEB: E2621S).

    Article Title: Induction of post-meiotic DNA double-strand breaks by the Pnu1 endonuclease in <i>Schizosaccharomyces pombe</i>
    Article Snippet: 1 Title Page Title: Induction of post-meiotic DNA double-strand breaks by the Pnu1 endonuclease in



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