ebfp2 mito7 (Addgene inc)
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ebfp2 mito7
Ebfp2 Mito7, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ebfp2+sequence/EBFP2-Mito-7+(Plasmid+%2355248)/pmc05716263-220-28-30
Average 93 stars, based on 8 article reviews
Ebfp2 Mito7, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ebfp2+sequence/EBFP2-Mito-7+(Plasmid+%2355248)/pmc05716263-220-28-30
Average 93 stars, based on 8 article reviews
ebfp2 mito7 - by Bioz Stars,
2026-11
93/100 stars
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Polymerase Chain Reaction:Article Title: Presynapses are mitophagy pit stops that prevent axon degeneration Article Snippet: .. Open reading frames (ORFs) of Article Title: In situ cryo-ET visualization of mitochondrial depolarization and mitophagic engulfment Article Snippet: To generate mCherry-Parkin and BFP-mito lentiviruses for cell line generation 4E6 HEK293T cells were seeded into 10 cm plates and transfected the next day with 45 μL Mirus LT1 transfection reagent (MIR2300) added to a mixture of 5 μg each (15 μg total) of plasmids VSV-G (addgene: 8454), R8.74 (addgene: 22036) or CMV-Gagpol (addgene: 35614), and one of the following: pLV-mCherry-Parkin (this study), pLV-BFP-mito (this study), and Su9-GFP-HALO (addgene: 184905) in 1.5 mL Optimem (ThermoFischer catalog: 31985062) according to manufacturer recommendations. .. Both lentivirus plasmids were restriction subcloned using NheI and BsrGI into pMK1253 (addgene: 133058) from pBMN-mCherry-Parkin (addgene: 59419, PCR amplified to include a c-terminal BsrGI cut-site) and Amplification:Article Title: Presynapses are mitophagy pit stops that prevent axon degeneration Article Snippet: .. Open reading frames (ORFs) of Article Title: In situ cryo-ET visualization of mitochondrial depolarization and mitophagic engulfment Article Snippet: To generate mCherry-Parkin and BFP-mito lentiviruses for cell line generation 4E6 HEK293T cells were seeded into 10 cm plates and transfected the next day with 45 μL Mirus LT1 transfection reagent (MIR2300) added to a mixture of 5 μg each (15 μg total) of plasmids VSV-G (addgene: 8454), R8.74 (addgene: 22036) or CMV-Gagpol (addgene: 35614), and one of the following: pLV-mCherry-Parkin (this study), pLV-BFP-mito (this study), and Su9-GFP-HALO (addgene: 184905) in 1.5 mL Optimem (ThermoFischer catalog: 31985062) according to manufacturer recommendations. .. Both lentivirus plasmids were restriction subcloned using NheI and BsrGI into pMK1253 (addgene: 133058) from pBMN-mCherry-Parkin (addgene: 59419, PCR amplified to include a c-terminal BsrGI cut-site) and Transfection:Article Title: IndiFluors: A New Full-Visible Color-Tunable Donor-Acceptor-Donor (D 1 -A-D 2 ) Fluorophore Family for Ratiometric pH Imaging during Mitophagy. Article Snippet: .. [13] Cellular Co-localization of PM-Mito-OH with Mito-BFP Experiment: HeLa cells were seeded in glass bottom confocal dish and after 24 hours, cells were transfected with Article Title: Decoupling SARS-CoV-2 ORF6 localization and interferon antagonism. Article Snippet: .. Roughly 6500 HeLa cells were seeded into an eight-well #1.5 glass bottom chamber slide (Ibidi #80826) and transfected with 100 ng of the indicated ORF6 expression construct along with either 50 ng of an Article Title: Decoupling SARS-CoV-2 ORF6 localization and interferon antagonism Article Snippet: .. Roughly 6,500 HeLa cells were seeded into an 8-well #1.5 glass bottom chamber slide (Ibidi #80826) and transfected with 100 ng of the indicated ORF6 expression construct along with either 50 ng of an Article Title: Methods for imaging mammalian mitochondrial morphology: a prospective on MitoGraph Article Snippet: .. For some transfection reagents, reducing the amount mito-YFP, and not using so-called “filler” DNA may reduce overall transfection efficiency. table ft1 table-wrap mode="anchored" t5 caption a7 Mitochondrial T argeted Fluorescent Protein Excitation Emission Addgene # Ref Fluorescent probes used to visualize Plasmid Preparation:Article Title: IndiFluors: A New Full-Visible Color-Tunable Donor-Acceptor-Donor (D 1 -A-D 2 ) Fluorophore Family for Ratiometric pH Imaging during Mitophagy. Article Snippet: .. [13] Cellular Co-localization of PM-Mito-OH with Mito-BFP Experiment: HeLa cells were seeded in glass bottom confocal dish and after 24 hours, cells were transfected with Article Title: Endosomes facilitate mitochondrial clearance by enhancing Parkin recruitment to mitochondria Article Snippet: .. Following constructs used were received from Addgene, YFP-Parkin was a gift from Richard Youle (Addgene plasmid # 23955) [ ], GFP-Rab5B and GFP-Rab7A were a gift from Gia Voeltz [ ], EBFP2-Mito-7 was a gift from Michael Davidson (Addgene plasmid # 55248) [ ], Staining:Article Title: IndiFluors: A New Full-Visible Color-Tunable Donor-Acceptor-Donor (D 1 -A-D 2 ) Fluorophore Family for Ratiometric pH Imaging during Mitophagy. Article Snippet: .. [13] Cellular Co-localization of PM-Mito-OH with Mito-BFP Experiment: HeLa cells were seeded in glass bottom confocal dish and after 24 hours, cells were transfected with Expressing:Article Title: Decoupling SARS-CoV-2 ORF6 localization and interferon antagonism. Article Snippet: .. Roughly 6500 HeLa cells were seeded into an eight-well #1.5 glass bottom chamber slide (Ibidi #80826) and transfected with 100 ng of the indicated ORF6 expression construct along with either 50 ng of an Article Title: Decoupling SARS-CoV-2 ORF6 localization and interferon antagonism Article Snippet: .. Roughly 6,500 HeLa cells were seeded into an 8-well #1.5 glass bottom chamber slide (Ibidi #80826) and transfected with 100 ng of the indicated ORF6 expression construct along with either 50 ng of an Construct:Article Title: Decoupling SARS-CoV-2 ORF6 localization and interferon antagonism. Article Snippet: .. Roughly 6500 HeLa cells were seeded into an eight-well #1.5 glass bottom chamber slide (Ibidi #80826) and transfected with 100 ng of the indicated ORF6 expression construct along with either 50 ng of an Article Title: Endosomes facilitate mitochondrial clearance by enhancing Parkin recruitment to mitochondria Article Snippet: .. Following constructs used were received from Addgene, YFP-Parkin was a gift from Richard Youle (Addgene plasmid # 23955) [ ], GFP-Rab5B and GFP-Rab7A were a gift from Gia Voeltz [ ], EBFP2-Mito-7 was a gift from Michael Davidson (Addgene plasmid # 55248) [ ], Article Title: Decoupling SARS-CoV-2 ORF6 localization and interferon antagonism Article Snippet: .. Roughly 6,500 HeLa cells were seeded into an 8-well #1.5 glass bottom chamber slide (Ibidi #80826) and transfected with 100 ng of the indicated ORF6 expression construct along with either 50 ng of an Marker:Article Title: Decoupling SARS-CoV-2 ORF6 localization and interferon antagonism. Article Snippet: .. Roughly 6500 HeLa cells were seeded into an eight-well #1.5 glass bottom chamber slide (Ibidi #80826) and transfected with 100 ng of the indicated ORF6 expression construct along with either 50 ng of an Article Title: Decoupling SARS-CoV-2 ORF6 localization and interferon antagonism Article Snippet: .. Roughly 6,500 HeLa cells were seeded into an 8-well #1.5 glass bottom chamber slide (Ibidi #80826) and transfected with 100 ng of the indicated ORF6 expression construct along with either 50 ng of an Fluorescence:Article Title: Methods for imaging mammalian mitochondrial morphology: a prospective on MitoGraph Article Snippet: .. For some transfection reagents, reducing the amount mito-YFP, and not using so-called “filler” DNA may reduce overall transfection efficiency. table ft1 table-wrap mode="anchored" t5 caption a7 Mitochondrial T argeted Fluorescent Protein Excitation Emission Addgene # Ref Fluorescent probes used to visualize |