ebfp sequence (Addgene inc)
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ebfp sequence
Ebfp Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ebfp+sequence/EBFP2-pBAD+(Plasmid+%2354542)/pmc04510296-358-10-14
Average 92 stars, based on 7 article reviews
Ebfp Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ebfp+sequence/EBFP2-pBAD+(Plasmid+%2354542)/pmc04510296-358-10-14
Average 92 stars, based on 7 article reviews
ebfp sequence - by Bioz Stars,
2026-09
92/100 stars
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Expressing:Article Title: Escherichia coli responds to environmental changes using enolasic degradosomes and stabilized DicF sRNA to alter cellular morphology Article Snippet: .. For ectopic expression of RNase E, we created pBAD-Rnewt plasmid by one-step in-frame insertion of the corresponding DNA fragment of the rne gene into the NdeI and EcoRI sites of Plasmid Preparation:Article Title: Escherichia coli responds to environmental changes using enolasic degradosomes and stabilized DicF sRNA to alter cellular morphology Article Snippet: .. For ectopic expression of RNase E, we created pBAD-Rnewt plasmid by one-step in-frame insertion of the corresponding DNA fragment of the rne gene into the NdeI and EcoRI sites of Article Title: Rediversification following ecotype isolation reveals hidden adaptive potential. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Various LTEE-derived E. coli strains This paper See Table S1 Chemicals, peptides, and recombinant proteins RNAse-free DNAse Invitrogen Catalogue number: AM2222 Ready-Lyse Lysozyme Solution Lucigen Catalogue number: R1804M Critical commercial assays Monarch Total RNA Miniprep Kit New England BioLabs Catalogue number: T2010S Stranded Total RNA Prep, Ligation with Ribo-Zero Plus Illumina Catalogue number: 20040529 DNeasy Blood and Tissue Kit Qiagen Catalogue number: 69504 NEBNext DNA Library Prep kit New England BioLabs Catalogue number: E7645 Nanopore ligation sequencing kit Oxford Nanopore Catalogue number: SQK-LSK109 Deposited data All DNA/RNA sequencing data This paper NCBI BioProject accession: PRJNA970313 REL606 genome Genbank NCBI RefSeq assembly: GCF_000017985.1 Processed data This paper https://github.com/joaoascensao/ Rediversification Oligonucleotides Oligonucleotides for genotyping and cloning This paper See Table S2 Article Title: AssemblX: a user-friendly toolkit for rapid and reliable multi-gene assemblies Article Snippet: Plasmids pCEV-G1-Ph and pCEV-G2-Km are gifts from Lars Nielsen and Claudia Vickers (Addgene plasmid # 46814 and 46815) ( ). pAG36 is a gift from John McCusker (Addgene plasmid # 35126) ( ). pHIS3p:mRuby2-Tub1+3 ́UTR::HPH is a gift from Wei-Lih Lee (Addgene plasmid # 50633). .. Polymerase Chain Reaction:Article Title: MAPT Genetic Variation and Neuronal Maturity Alter Isoform Expression Affecting Axonal Transport in iPSC-Derived Dopamine Neurons Article Snippet: .. The following DNA molecules were joined using Gibson Assembly Master Mix (NEB): pRRL.sin.wpre fragment of SpeI-HF/SalI-HF double digest (NEB) of pRRL.sin.U6.shRNA.cPPT.CMV.EGFP.wpre (kind gift from Dr Óscar Cordero Llana); PCR of U6 promoter from the same construct, adding a specific shRNA sequence with the reverse primer; PCR of RRE.cPPT.pEF1α from CSii-EF-MCS (a gift from Dr H. Miyoshi, RIKEN BioResource Center DNA Bank); PCR of EBFP2 from Article Title: MAPT Genetic Variation and Neuronal Maturity Alter Isoform Expression Affecting Axonal Transport in iPSC-Derived Dopamine Neurons Article Snippet: .. The following DNA molecules were joined using Gibson Assembly Master Mix (NEB): pRRL.sin.wpre fragment of SpeI-HF/SalI-HF double digest (NEB) of pRRL.sin.U6.shRNA.cPPT.CMV.EGFP.wpre (kind gift from Dr Óscar Cordero Llana); PCR of U6 promoter from the same construct, adding a specific shRNA sequence with the reverse primer; PCR of RRE.cPPT.pEF1α from CSii-EF-MCS (a gift from Dr H. Miyoshi, RIKEN BioResource Center DNA Bank); PCR of EBFP2 from Construct:Article Title: MAPT Genetic Variation and Neuronal Maturity Alter Isoform Expression Affecting Axonal Transport in iPSC-Derived Dopamine Neurons Article Snippet: .. The following DNA molecules were joined using Gibson Assembly Master Mix (NEB): pRRL.sin.wpre fragment of SpeI-HF/SalI-HF double digest (NEB) of pRRL.sin.U6.shRNA.cPPT.CMV.EGFP.wpre (kind gift from Dr Óscar Cordero Llana); PCR of U6 promoter from the same construct, adding a specific shRNA sequence with the reverse primer; PCR of RRE.cPPT.pEF1α from CSii-EF-MCS (a gift from Dr H. Miyoshi, RIKEN BioResource Center DNA Bank); PCR of EBFP2 from Article Title: MAPT Genetic Variation and Neuronal Maturity Alter Isoform Expression Affecting Axonal Transport in iPSC-Derived Dopamine Neurons Article Snippet: .. The following DNA molecules were joined using Gibson Assembly Master Mix (NEB): pRRL.sin.wpre fragment of SpeI-HF/SalI-HF double digest (NEB) of pRRL.sin.U6.shRNA.cPPT.CMV.EGFP.wpre (kind gift from Dr Óscar Cordero Llana); PCR of U6 promoter from the same construct, adding a specific shRNA sequence with the reverse primer; PCR of RRE.cPPT.pEF1α from CSii-EF-MCS (a gift from Dr H. Miyoshi, RIKEN BioResource Center DNA Bank); PCR of EBFP2 from Article Title: VPS35 in Dopamine Neurons Is Required for Endosome-to-Golgi Retrieval of Lamp2a, a Receptor of Chaperone-Mediated Autophagy That Is Critical for α-Synuclein Degradation and Prevention of Pathogenesis of Parkinson's Disease Article Snippet: .. The miRNA-VPS35-eBFP was constructed by replacing the EmGFP with the eBFP sequence from shRNA:Article Title: MAPT Genetic Variation and Neuronal Maturity Alter Isoform Expression Affecting Axonal Transport in iPSC-Derived Dopamine Neurons Article Snippet: .. The following DNA molecules were joined using Gibson Assembly Master Mix (NEB): pRRL.sin.wpre fragment of SpeI-HF/SalI-HF double digest (NEB) of pRRL.sin.U6.shRNA.cPPT.CMV.EGFP.wpre (kind gift from Dr Óscar Cordero Llana); PCR of U6 promoter from the same construct, adding a specific shRNA sequence with the reverse primer; PCR of RRE.cPPT.pEF1α from CSii-EF-MCS (a gift from Dr H. Miyoshi, RIKEN BioResource Center DNA Bank); PCR of EBFP2 from Article Title: MAPT Genetic Variation and Neuronal Maturity Alter Isoform Expression Affecting Axonal Transport in iPSC-Derived Dopamine Neurons Article Snippet: .. The following DNA molecules were joined using Gibson Assembly Master Mix (NEB): pRRL.sin.wpre fragment of SpeI-HF/SalI-HF double digest (NEB) of pRRL.sin.U6.shRNA.cPPT.CMV.EGFP.wpre (kind gift from Dr Óscar Cordero Llana); PCR of U6 promoter from the same construct, adding a specific shRNA sequence with the reverse primer; PCR of RRE.cPPT.pEF1α from CSii-EF-MCS (a gift from Dr H. Miyoshi, RIKEN BioResource Center DNA Bank); PCR of EBFP2 from Sequencing:Article Title: MAPT Genetic Variation and Neuronal Maturity Alter Isoform Expression Affecting Axonal Transport in iPSC-Derived Dopamine Neurons Article Snippet: .. The following DNA molecules were joined using Gibson Assembly Master Mix (NEB): pRRL.sin.wpre fragment of SpeI-HF/SalI-HF double digest (NEB) of pRRL.sin.U6.shRNA.cPPT.CMV.EGFP.wpre (kind gift from Dr Óscar Cordero Llana); PCR of U6 promoter from the same construct, adding a specific shRNA sequence with the reverse primer; PCR of RRE.cPPT.pEF1α from CSii-EF-MCS (a gift from Dr H. Miyoshi, RIKEN BioResource Center DNA Bank); PCR of EBFP2 from Article Title: Rediversification following ecotype isolation reveals hidden adaptive potential. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Various LTEE-derived E. coli strains This paper See Table S1 Chemicals, peptides, and recombinant proteins RNAse-free DNAse Invitrogen Catalogue number: AM2222 Ready-Lyse Lysozyme Solution Lucigen Catalogue number: R1804M Critical commercial assays Monarch Total RNA Miniprep Kit New England BioLabs Catalogue number: T2010S Stranded Total RNA Prep, Ligation with Ribo-Zero Plus Illumina Catalogue number: 20040529 DNeasy Blood and Tissue Kit Qiagen Catalogue number: 69504 NEBNext DNA Library Prep kit New England BioLabs Catalogue number: E7645 Nanopore ligation sequencing kit Oxford Nanopore Catalogue number: SQK-LSK109 Deposited data All DNA/RNA sequencing data This paper NCBI BioProject accession: PRJNA970313 REL606 genome Genbank NCBI RefSeq assembly: GCF_000017985.1 Processed data This paper https://github.com/joaoascensao/ Rediversification Oligonucleotides Oligonucleotides for genotyping and cloning This paper See Table S2 Article Title: MAPT Genetic Variation and Neuronal Maturity Alter Isoform Expression Affecting Axonal Transport in iPSC-Derived Dopamine Neurons Article Snippet: .. The following DNA molecules were joined using Gibson Assembly Master Mix (NEB): pRRL.sin.wpre fragment of SpeI-HF/SalI-HF double digest (NEB) of pRRL.sin.U6.shRNA.cPPT.CMV.EGFP.wpre (kind gift from Dr Óscar Cordero Llana); PCR of U6 promoter from the same construct, adding a specific shRNA sequence with the reverse primer; PCR of RRE.cPPT.pEF1α from CSii-EF-MCS (a gift from Dr H. Miyoshi, RIKEN BioResource Center DNA Bank); PCR of EBFP2 from Article Title: VPS35 in Dopamine Neurons Is Required for Endosome-to-Golgi Retrieval of Lamp2a, a Receptor of Chaperone-Mediated Autophagy That Is Critical for α-Synuclein Degradation and Prevention of Pathogenesis of Parkinson's Disease Article Snippet: .. The miRNA-VPS35-eBFP was constructed by replacing the EmGFP with the eBFP sequence from Virus:Article Title: Rediversification following ecotype isolation reveals hidden adaptive potential. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Various LTEE-derived E. coli strains This paper See Table S1 Chemicals, peptides, and recombinant proteins RNAse-free DNAse Invitrogen Catalogue number: AM2222 Ready-Lyse Lysozyme Solution Lucigen Catalogue number: R1804M Critical commercial assays Monarch Total RNA Miniprep Kit New England BioLabs Catalogue number: T2010S Stranded Total RNA Prep, Ligation with Ribo-Zero Plus Illumina Catalogue number: 20040529 DNeasy Blood and Tissue Kit Qiagen Catalogue number: 69504 NEBNext DNA Library Prep kit New England BioLabs Catalogue number: E7645 Nanopore ligation sequencing kit Oxford Nanopore Catalogue number: SQK-LSK109 Deposited data All DNA/RNA sequencing data This paper NCBI BioProject accession: PRJNA970313 REL606 genome Genbank NCBI RefSeq assembly: GCF_000017985.1 Processed data This paper https://github.com/joaoascensao/ Rediversification Oligonucleotides Oligonucleotides for genotyping and cloning This paper See Table S2 Recombinant:Article Title: Rediversification following ecotype isolation reveals hidden adaptive potential. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Various LTEE-derived E. coli strains This paper See Table S1 Chemicals, peptides, and recombinant proteins RNAse-free DNAse Invitrogen Catalogue number: AM2222 Ready-Lyse Lysozyme Solution Lucigen Catalogue number: R1804M Critical commercial assays Monarch Total RNA Miniprep Kit New England BioLabs Catalogue number: T2010S Stranded Total RNA Prep, Ligation with Ribo-Zero Plus Illumina Catalogue number: 20040529 DNeasy Blood and Tissue Kit Qiagen Catalogue number: 69504 NEBNext DNA Library Prep kit New England BioLabs Catalogue number: E7645 Nanopore ligation sequencing kit Oxford Nanopore Catalogue number: SQK-LSK109 Deposited data All DNA/RNA sequencing data This paper NCBI BioProject accession: PRJNA970313 REL606 genome Genbank NCBI RefSeq assembly: GCF_000017985.1 Processed data This paper https://github.com/joaoascensao/ Rediversification Oligonucleotides Oligonucleotides for genotyping and cloning This paper See Table S2 Ligation:Article Title: Rediversification following ecotype isolation reveals hidden adaptive potential. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Various LTEE-derived E. coli strains This paper See Table S1 Chemicals, peptides, and recombinant proteins RNAse-free DNAse Invitrogen Catalogue number: AM2222 Ready-Lyse Lysozyme Solution Lucigen Catalogue number: R1804M Critical commercial assays Monarch Total RNA Miniprep Kit New England BioLabs Catalogue number: T2010S Stranded Total RNA Prep, Ligation with Ribo-Zero Plus Illumina Catalogue number: 20040529 DNeasy Blood and Tissue Kit Qiagen Catalogue number: 69504 NEBNext DNA Library Prep kit New England BioLabs Catalogue number: E7645 Nanopore ligation sequencing kit Oxford Nanopore Catalogue number: SQK-LSK109 Deposited data All DNA/RNA sequencing data This paper NCBI BioProject accession: PRJNA970313 REL606 genome Genbank NCBI RefSeq assembly: GCF_000017985.1 Processed data This paper https://github.com/joaoascensao/ Rediversification Oligonucleotides Oligonucleotides for genotyping and cloning This paper See Table S2 Cloning:Article Title: Rediversification following ecotype isolation reveals hidden adaptive potential. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Various LTEE-derived E. coli strains This paper See Table S1 Chemicals, peptides, and recombinant proteins RNAse-free DNAse Invitrogen Catalogue number: AM2222 Ready-Lyse Lysozyme Solution Lucigen Catalogue number: R1804M Critical commercial assays Monarch Total RNA Miniprep Kit New England BioLabs Catalogue number: T2010S Stranded Total RNA Prep, Ligation with Ribo-Zero Plus Illumina Catalogue number: 20040529 DNeasy Blood and Tissue Kit Qiagen Catalogue number: 69504 NEBNext DNA Library Prep kit New England BioLabs Catalogue number: E7645 Nanopore ligation sequencing kit Oxford Nanopore Catalogue number: SQK-LSK109 Deposited data All DNA/RNA sequencing data This paper NCBI BioProject accession: PRJNA970313 REL606 genome Genbank NCBI RefSeq assembly: GCF_000017985.1 Processed data This paper https://github.com/joaoascensao/ Rediversification Oligonucleotides Oligonucleotides for genotyping and cloning This paper See Table S2 Software:Article Title: Rediversification following ecotype isolation reveals hidden adaptive potential. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Various LTEE-derived E. coli strains This paper See Table S1 Chemicals, peptides, and recombinant proteins RNAse-free DNAse Invitrogen Catalogue number: AM2222 Ready-Lyse Lysozyme Solution Lucigen Catalogue number: R1804M Critical commercial assays Monarch Total RNA Miniprep Kit New England BioLabs Catalogue number: T2010S Stranded Total RNA Prep, Ligation with Ribo-Zero Plus Illumina Catalogue number: 20040529 DNeasy Blood and Tissue Kit Qiagen Catalogue number: 69504 NEBNext DNA Library Prep kit New England BioLabs Catalogue number: E7645 Nanopore ligation sequencing kit Oxford Nanopore Catalogue number: SQK-LSK109 Deposited data All DNA/RNA sequencing data This paper NCBI BioProject accession: PRJNA970313 REL606 genome Genbank NCBI RefSeq assembly: GCF_000017985.1 Processed data This paper https://github.com/joaoascensao/ Rediversification Oligonucleotides Oligonucleotides for genotyping and cloning This paper See Table S2 Flow Cytometry:Article Title: Rediversification following ecotype isolation reveals hidden adaptive potential. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Various LTEE-derived E. coli strains This paper See Table S1 Chemicals, peptides, and recombinant proteins RNAse-free DNAse Invitrogen Catalogue number: AM2222 Ready-Lyse Lysozyme Solution Lucigen Catalogue number: R1804M Critical commercial assays Monarch Total RNA Miniprep Kit New England BioLabs Catalogue number: T2010S Stranded Total RNA Prep, Ligation with Ribo-Zero Plus Illumina Catalogue number: 20040529 DNeasy Blood and Tissue Kit Qiagen Catalogue number: 69504 NEBNext DNA Library Prep kit New England BioLabs Catalogue number: E7645 Nanopore ligation sequencing kit Oxford Nanopore Catalogue number: SQK-LSK109 Deposited data All DNA/RNA sequencing data This paper NCBI BioProject accession: PRJNA970313 REL606 genome Genbank NCBI RefSeq assembly: GCF_000017985.1 Processed data This paper https://github.com/joaoascensao/ Rediversification Oligonucleotides Oligonucleotides for genotyping and cloning This paper See Table S2 |