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American Life Science Pharmaceuticals 99% pure e64d
99% Pure E64d, supplied by American Life Science Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e64d/e64d/pmc04059604-81-2-7
Average 90 stars, based on 1 article reviews
99% pure e64d - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Brain Pyroglutamate Amyloid-Beta is Produced by Cathepsin B and is Reduced by the Cysteine Protease Inhibitor E64d, Representing a Potential Alzheimer’s Disease Therapeutic
Article Snippet: Briefly, 99% pure E64d was made by American Life Science Pharmaceuticals (San Diego, CA) using methods developed and modified from those described [ 64 ].

Article Title: Compositions and methods for treating beta-amyloid related diseases
Article Snippet: Materials and Methods AB-007 (E64d, loxistatin) AB-007 (E64d, loxistatin) was synthesized by American Life Science Pharmaceuticals (San Diego, Calif.).

Synthesized:

Article Title: The cysteine protease inhibitor, E64d, reduces brain amyloid-β and improves memory deficits in Alzheimer’s disease animal models by inhibiting cathepsin B, but not BACE1, β-secretase activity
Article Snippet: .. E64d was synthesized by American Life Science Pharmaceuticals (San Diego, CA) using methods developed and modified from those previously described[ 46 ]. ..

Modification:

Article Title: The cysteine protease inhibitor, E64d, reduces brain amyloid-β and improves memory deficits in Alzheimer’s disease animal models by inhibiting cathepsin B, but not BACE1, β-secretase activity
Article Snippet: .. E64d was synthesized by American Life Science Pharmaceuticals (San Diego, CA) using methods developed and modified from those previously described[ 46 ]. ..

Article Title: The Cysteine Protease Cathepsin B Is a Key Drug Target and Cysteine Protease Inhibitors Are Potential Therapeutics for Traumatic Brain Injury
Article Snippet: .. E64d E64d was manufactured by American Life Science Pharmaceuticals (San Diego, CA) using methods developed and modified from those previously described. ..

Article Title: Brain Pyroglutamate Amyloid-β is Produced by Cathepsin B and is Reduced by the Cysteine Protease Inhibitor E64d, Representing a Potential Alzheimer's Disease Therapeutic
Article Snippet: .. Briefly, 99% pure E64d was made by American Life Science Pharmaceuticals (San Diego, CA) using methods developed and modified from those described [64]. .. However, E64d is also commercially available from various suppliers (e.g., Calbiochem, San Diego, CA).

Article Title: The Cysteine Protease Cathepsin B Is a Key Drug Target and Cysteine Protease Inhibitors Are Potential Therapeutics for Traumatic Brain Injury
Article Snippet: .. E64d was manufactured by American Life Science Pharmaceuticals (San Diego, CA) using methods developed and modified from those previously described. ..

Transgenic Assay:

Article Title: The Cysteine Protease Inhibitor, E64d, Reduces Brain Amyloid-β and Improves Memory Deficits in Alzheimer's Disease Animal Models by Inhibiting Cathepsin B, but not BACE1, β-Secretase Activity
Article Snippet: .. Keywords: Alzheimer disease, amyloid- , BACE1, cathepsin B, guinea pig, memory deficits, oral E64d, transgenic mice ∗Correspondence to: Gregory Hook, Ph.D., American Life Science Pharmaceuticals, 3030 Bunker Hill Street, Suite 104, San Diego, CA 92109. ..



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Neutralization curves of VSV pseudoviruses carrying FL, Δ13, and Δ17 spike to the indicated monoclonal neutralizing antibodies ( a ), serum samples collected after SARS-CoV-2 pandemic ( b ), hACE2 ( c ), and the protease inhibitors <t>E64d</t> and camostat ( d ). Antibody and serum neutralization assays, as well as hACE2 inhibition assays, were performed in Vero E6 cells, whereas protease inhibitor assays were conducted in Vero E6-ACE2-TMPRSS2 cells. Numbers in each panel denote IC 50 or ID 50 of pseudoviruses carrying FL (black), Δ13 (red), and Δ17 (blue) spikes.
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Neutralization curves of VSV pseudoviruses carrying FL, Δ13, and Δ17 spike to the indicated monoclonal neutralizing antibodies ( a ), serum samples collected after SARS-CoV-2 pandemic ( b ), hACE2 ( c ), and the protease inhibitors <t>E64d</t> and camostat ( d ). Antibody and serum neutralization assays, as well as hACE2 inhibition assays, were performed in Vero E6 cells, whereas protease inhibitor assays were conducted in Vero E6-ACE2-TMPRSS2 cells. Numbers in each panel denote IC 50 or ID 50 of pseudoviruses carrying FL (black), Δ13 (red), and Δ17 (blue) spikes.
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Neutralization curves of VSV pseudoviruses carrying FL, Δ13, and Δ17 spike to the indicated monoclonal neutralizing antibodies ( a ), serum samples collected after SARS-CoV-2 pandemic ( b ), hACE2 ( c ), and the protease inhibitors E64d and camostat ( d ). Antibody and serum neutralization assays, as well as hACE2 inhibition assays, were performed in Vero E6 cells, whereas protease inhibitor assays were conducted in Vero E6-ACE2-TMPRSS2 cells. Numbers in each panel denote IC 50 or ID 50 of pseudoviruses carrying FL (black), Δ13 (red), and Δ17 (blue) spikes.

Journal: bioRxiv

Article Title: Functional Landscape of Motifs within the Sarbecovirus Spike Cytoplasmic Tail

doi: 10.64898/2026.05.06.723231

Figure Lengend Snippet: Neutralization curves of VSV pseudoviruses carrying FL, Δ13, and Δ17 spike to the indicated monoclonal neutralizing antibodies ( a ), serum samples collected after SARS-CoV-2 pandemic ( b ), hACE2 ( c ), and the protease inhibitors E64d and camostat ( d ). Antibody and serum neutralization assays, as well as hACE2 inhibition assays, were performed in Vero E6 cells, whereas protease inhibitor assays were conducted in Vero E6-ACE2-TMPRSS2 cells. Numbers in each panel denote IC 50 or ID 50 of pseudoviruses carrying FL (black), Δ13 (red), and Δ17 (blue) spikes.

Article Snippet: To test the effect of host protease inhibitors on pseudovirus entry, Vero E6-ACE2-TMPRSS2 cells (4×104 cells/well) were pre-incubated for 2 hours with serially diluted cathepsin L inhibitor E64d (MedChemExpress, CAS# 88321-09-9) or TMPRSS2 inhibitor camostat mesylate (MedChemExpress, CAS# 59721-29-8).

Techniques: Neutralization, Inhibition, Protease Inhibitor