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e2f 1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc e2f 1
    E2f 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 682 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2f/E2F-1+Antibody/pmc12892985-6-0-4
    Average 96 stars, based on 682 article reviews
    e2f 1 - by Bioz Stars, 2026-11
    96/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: Separate transcription and splicing gene networks are linked and coordinated by the pRb–E2F pathway
    Article Snippet: .. The following antibodies were used in immunoblots: β-actin (clone AC-74, Sigma–Aldrich, St. Louis, USA), E2F1 (3742S, Cell Signaling Technology, Danvers, USA), GAPDH (clone 6C5, MAB374, Millipore, Burlington, USA), HNRNPC1/C2 (clone 4F4, sc-32308, Santa Cruz, Dallas, USA), SC35 (EPR12238, ab204916, Abcam, Cambridge, UK), SRSF2 (PA5-12402, Thermo Fisher, Waltham, USA), pRb (clone 4H1, 9309S, Cell Signaling Technology, Danvers, USA), DP-1 (clone TFD-10, sc-53642, Santa Cruz, Dallas, USA), and symmetric di-methyl arginine (SDMe) (13222S, Cell Signaling Technology, Danvers, USA). ..

    Article Title: M1C Mediates LINE-1 Transcription in PARP Inhibitor-Treated Prostate Cancer Cells.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Cell-Signaling:

    Article Title: Separate transcription and splicing gene networks are linked and coordinated by the pRb–E2F pathway
    Article Snippet: .. The following antibodies were used in immunoblots: β-actin (clone AC-74, Sigma–Aldrich, St. Louis, USA), E2F1 (3742S, Cell Signaling Technology, Danvers, USA), GAPDH (clone 6C5, MAB374, Millipore, Burlington, USA), HNRNPC1/C2 (clone 4F4, sc-32308, Santa Cruz, Dallas, USA), SC35 (EPR12238, ab204916, Abcam, Cambridge, UK), SRSF2 (PA5-12402, Thermo Fisher, Waltham, USA), pRb (clone 4H1, 9309S, Cell Signaling Technology, Danvers, USA), DP-1 (clone TFD-10, sc-53642, Santa Cruz, Dallas, USA), and symmetric di-methyl arginine (SDMe) (13222S, Cell Signaling Technology, Danvers, USA). ..

    Article Title: A CDK-4EBP1 signaling axis drives HSV-1 replication and underscores a druggable pathway for potent antiviral intervention
    Article Snippet: Phospho-Rb (Ser608) , #2181 , Cell Signaling Technologies, USA , 110. .. E2F-1 , #3742 , Cell Signaling Technologies, USA , 70. .. Phospho-RB-S780 , AP0444 , ABclonal, USA , 100.

    Blocking Assay:

    Article Title: Cuproptosis-associated PDHA1 promotes sarcoma progression and immunotherapy responsiveness via the E2F1-PD-L1 axis: a multi-omics and clinical validation study.
    Article Snippet: .. After blocking with 5% non-fat milk for 1 h at room temperature, membranes were incubated overnight at 4 °C with primary antibodies against PDHA1 (ab168379, Abcam), E2F1 (#3742, CST), PD-L1 (#13684, CST), Bcl-2 (ab182858, Abcam), MMP-9 (ab76003, Abcam), cleaved Caspase-3 (#9661, CST), and lipoylated DLAT (anti-lipoic acid rabbit pAb, 437695, Millipore), as well as GAPDH (ab8245, Abcam; 1:2,000) as a loading control. .. After incubation with HRP-conjugated secondary antibodies for 1 h at room temperature, protein bands were visualized using enhanced chemiluminescence reagents (ECL, Millipore) and quantified using ImageJ software.

    Incubation:

    Article Title: Cuproptosis-associated PDHA1 promotes sarcoma progression and immunotherapy responsiveness via the E2F1-PD-L1 axis: a multi-omics and clinical validation study.
    Article Snippet: .. After blocking with 5% non-fat milk for 1 h at room temperature, membranes were incubated overnight at 4 °C with primary antibodies against PDHA1 (ab168379, Abcam), E2F1 (#3742, CST), PD-L1 (#13684, CST), Bcl-2 (ab182858, Abcam), MMP-9 (ab76003, Abcam), cleaved Caspase-3 (#9661, CST), and lipoylated DLAT (anti-lipoic acid rabbit pAb, 437695, Millipore), as well as GAPDH (ab8245, Abcam; 1:2,000) as a loading control. .. After incubation with HRP-conjugated secondary antibodies for 1 h at room temperature, protein bands were visualized using enhanced chemiluminescence reagents (ECL, Millipore) and quantified using ImageJ software.

    Article Title: “Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”
    Article Snippet: .. Cells were then incubated overnight at 4 °C with the following primary antibodies: LARP1 (Cat. No. sc-515873; 1:200 dilution; Santa Cruz Biotechnology, Dallas, TX, USA) and E2F1 (Cat. No. 3742S; 1:400 dilution; Cell Signaling Technology, Danvers, MA, USA). .. After washing with PBS, cells were incubated for 1 h at room temperature with secondary antibodies: goat anti-mouse IgG Alexa Fluor 488 (Cat. No. A-11001) and goat anti-rabbit IgG Alexa Fluor 568 (Cat. No. A-11011) (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA).

    Control:

    Article Title: Cuproptosis-associated PDHA1 promotes sarcoma progression and immunotherapy responsiveness via the E2F1-PD-L1 axis: a multi-omics and clinical validation study.
    Article Snippet: .. After blocking with 5% non-fat milk for 1 h at room temperature, membranes were incubated overnight at 4 °C with primary antibodies against PDHA1 (ab168379, Abcam), E2F1 (#3742, CST), PD-L1 (#13684, CST), Bcl-2 (ab182858, Abcam), MMP-9 (ab76003, Abcam), cleaved Caspase-3 (#9661, CST), and lipoylated DLAT (anti-lipoic acid rabbit pAb, 437695, Millipore), as well as GAPDH (ab8245, Abcam; 1:2,000) as a loading control. .. After incubation with HRP-conjugated secondary antibodies for 1 h at room temperature, protein bands were visualized using enhanced chemiluminescence reagents (ECL, Millipore) and quantified using ImageJ software.

    Article Title: Multi-omics profiling reveals homologous recombination repair hyperactivation as a therapeutic vulnerability in neuroendocrine cervical carcinoma.
    Article Snippet: Chromatin was sheared to 200–500 bp fragments using a Covaris ultrasonicator. .. After preclearing with Protein G magnetic beads, chromatin was immunoprecipitated with anti-E2F1 antibody (CST, #3742) or control IgG overnight at 4 °C. ..

    Magnetic Beads:

    Article Title: Multi-omics profiling reveals homologous recombination repair hyperactivation as a therapeutic vulnerability in neuroendocrine cervical carcinoma.
    Article Snippet: Chromatin was sheared to 200–500 bp fragments using a Covaris ultrasonicator. .. After preclearing with Protein G magnetic beads, chromatin was immunoprecipitated with anti-E2F1 antibody (CST, #3742) or control IgG overnight at 4 °C. ..

    Immunoprecipitation:

    Article Title: Multi-omics profiling reveals homologous recombination repair hyperactivation as a therapeutic vulnerability in neuroendocrine cervical carcinoma.
    Article Snippet: Chromatin was sheared to 200–500 bp fragments using a Covaris ultrasonicator. .. After preclearing with Protein G magnetic beads, chromatin was immunoprecipitated with anti-E2F1 antibody (CST, #3742) or control IgG overnight at 4 °C. ..



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    Image Search Results


    (A, B) Kaplan–Meier plot showing that overexpression of E2F1 is associated with shorter overall survival (A) and progression-free interval (B) in endometrial cancer cohort. (C, D) Kaplan–Meier plot showing that co-overexpression of LARP1 and E2F1 is associated with shorter overall survival (C) and progression-free interval (D) in endometrial cancer cohort. (E) Spearman correlation analysis shows a correlation between LARP1 and E2F1 in endometrial cancer patients.

    Journal: bioRxiv

    Article Title: “Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”

    doi: 10.64898/2026.03.22.713473

    Figure Lengend Snippet: (A, B) Kaplan–Meier plot showing that overexpression of E2F1 is associated with shorter overall survival (A) and progression-free interval (B) in endometrial cancer cohort. (C, D) Kaplan–Meier plot showing that co-overexpression of LARP1 and E2F1 is associated with shorter overall survival (C) and progression-free interval (D) in endometrial cancer cohort. (E) Spearman correlation analysis shows a correlation between LARP1 and E2F1 in endometrial cancer patients.

    Article Snippet: Cells were then incubated overnight at 4 °C with the following primary antibodies: LARP1 (Cat. No. sc-515873; 1:200 dilution; Santa Cruz Biotechnology, Dallas, TX, USA) and E2F1 (Cat. No. 3742S; 1:400 dilution; Cell Signaling Technology, Danvers, MA, USA).

    Techniques: Over Expression

    (A, B) Immunoblot analysis showing the protein expression of LARP1 and E2F1 after transfecting ISHI (A) and HEC-1A (B) cells with control or LARP1 siRNA. β-actin was used as a loading control. (C) Images representing immunofluorescence staining of LARP1 (green) and E2F1 (red) in HEC-1A cells after transfection with control or LARP1 siRNA. DAPI was used as a counter stain. Scale bar = 50 µm.

    Journal: bioRxiv

    Article Title: “Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”

    doi: 10.64898/2026.03.22.713473

    Figure Lengend Snippet: (A, B) Immunoblot analysis showing the protein expression of LARP1 and E2F1 after transfecting ISHI (A) and HEC-1A (B) cells with control or LARP1 siRNA. β-actin was used as a loading control. (C) Images representing immunofluorescence staining of LARP1 (green) and E2F1 (red) in HEC-1A cells after transfection with control or LARP1 siRNA. DAPI was used as a counter stain. Scale bar = 50 µm.

    Article Snippet: Cells were then incubated overnight at 4 °C with the following primary antibodies: LARP1 (Cat. No. sc-515873; 1:200 dilution; Santa Cruz Biotechnology, Dallas, TX, USA) and E2F1 (Cat. No. 3742S; 1:400 dilution; Cell Signaling Technology, Danvers, MA, USA).

    Techniques: Western Blot, Expressing, Control, Immunofluorescence, Staining, Transfection

    (A) Phylogenetic analysis of the E2F/DP gene family in tomato, rice, soybean, maize, and Arabidopsis thaliana . The E2F/DP genes were classified into three major groups: E2F-type, DEL-type, and DP-type. (B) Chromosomal localization of OsE2F/DP genes. Chromosome numbers are shown at the top, and the scale on the left indicates physical distance in megabases (Mb). A genetic interval of approximately 350 kb was set to accurately illustrate the distribution of the genes.

    Journal: Frontiers in Plant Science

    Article Title: Identification of E2F/DP gene family and their expression analysis under cold stress in rice

    doi: 10.3389/fpls.2026.1750382

    Figure Lengend Snippet: (A) Phylogenetic analysis of the E2F/DP gene family in tomato, rice, soybean, maize, and Arabidopsis thaliana . The E2F/DP genes were classified into three major groups: E2F-type, DEL-type, and DP-type. (B) Chromosomal localization of OsE2F/DP genes. Chromosome numbers are shown at the top, and the scale on the left indicates physical distance in megabases (Mb). A genetic interval of approximately 350 kb was set to accurately illustrate the distribution of the genes.

    Article Snippet: In Medicago, salt stress has been shown to promote the co-expression of E2F and DP genes ( ).

    Techniques:

    Conserved domains, motif patterns, and gene structures of rice E2F/DP genes. (A) The motif patterns of OsE2F/DP members are shown. (B) Conserved domains are indicated by colored boxes, with four conserved domains represented by distinct colors. The scale bar at the bottom of the panel allows estimation of the relative lengths of gene structures and motif components. (C) The gene structures of OsE2F/DP genes are illustrated, where green boxes represent the untranslated regions (UTRs) at the 5′ and 3′ ends, yellow boxes denote the coding sequences (CDSs), and black lines indicate introns.

    Journal: Frontiers in Plant Science

    Article Title: Identification of E2F/DP gene family and their expression analysis under cold stress in rice

    doi: 10.3389/fpls.2026.1750382

    Figure Lengend Snippet: Conserved domains, motif patterns, and gene structures of rice E2F/DP genes. (A) The motif patterns of OsE2F/DP members are shown. (B) Conserved domains are indicated by colored boxes, with four conserved domains represented by distinct colors. The scale bar at the bottom of the panel allows estimation of the relative lengths of gene structures and motif components. (C) The gene structures of OsE2F/DP genes are illustrated, where green boxes represent the untranslated regions (UTRs) at the 5′ and 3′ ends, yellow boxes denote the coding sequences (CDSs), and black lines indicate introns.

    Article Snippet: In Medicago, salt stress has been shown to promote the co-expression of E2F and DP genes ( ).

    Techniques:

    (A) Collinearity analysis of homologous OsE2F/DP genes in rice. In the figure, syntenic blocks across the entire rice genome are shown with a gray background, while red curves connect the pairs of homologous OsE2F/DP genes. (B) Synteny relationships of E2F/DP genes among Arabidopsis thaliana , rice, and sorghum. Gray lines in the background represent collinear gene pairs between the two species, whereas the E2F/DP collinear gene pairs are highlighted with reddish-brown lines.

    Journal: Frontiers in Plant Science

    Article Title: Identification of E2F/DP gene family and their expression analysis under cold stress in rice

    doi: 10.3389/fpls.2026.1750382

    Figure Lengend Snippet: (A) Collinearity analysis of homologous OsE2F/DP genes in rice. In the figure, syntenic blocks across the entire rice genome are shown with a gray background, while red curves connect the pairs of homologous OsE2F/DP genes. (B) Synteny relationships of E2F/DP genes among Arabidopsis thaliana , rice, and sorghum. Gray lines in the background represent collinear gene pairs between the two species, whereas the E2F/DP collinear gene pairs are highlighted with reddish-brown lines.

    Article Snippet: In Medicago, salt stress has been shown to promote the co-expression of E2F and DP genes ( ).

    Techniques: