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bmk-m-48.48gt  (fluidigm)


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    Structured Review

    fluidigm bmk-m-48.48gt
    Bmk M 48.48gt, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dynamic/48%2E48+Dynamic+Array+IFC+for+Genotyping/pmc08319808-76-9-7
    Average 95 stars, based on 185 article reviews
    bmk-m-48.48gt - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Overlap Extension Polymerase Chain Reaction:

    Article Title: in situ Transformation of Information Into DNA Storage With Microfluidic Very Large-Scale Integration Platform.
    Article Snippet: .. The DNA data con- sisted of the pooled products of all three OE-PCR reactions. (S4, Supporting Information) To verify the specificity of the assembled DNA sequences, we retrieved the DNA information with next-generation sequencing and with Standard BioTools 48.48 Dynamic Array IFC. ..

    Next-Generation Sequencing:

    Article Title: in situ Transformation of Information Into DNA Storage With Microfluidic Very Large-Scale Integration Platform.
    Article Snippet: .. The DNA data con- sisted of the pooled products of all three OE-PCR reactions. (S4, Supporting Information) To verify the specificity of the assembled DNA sequences, we retrieved the DNA information with next-generation sequencing and with Standard BioTools 48.48 Dynamic Array IFC. ..

    Article Title: in situ Transformation of Information Into DNA Storage With Microfluidic Very Large‐Scale Integration Platform
    Article Snippet: .. The DNA data consisted of the pooled products of all three OE‐PCR reactions. ( , Supporting Information) To verify the specificity of the assembled DNA sequences, we retrieved the DNA information with next‐generation sequencing and with Standard BioTools 48.48 Dynamic Array IFC. ..

    Real-time Polymerase Chain Reaction:

    Article Title: KREH2 helicase represses ND7 mRNA editing in procyclic-stage Trypanosoma brucei by opposite modulation of canonical and 'moonlighting' gRNA utilization creating a proposed mRNA structure.
    Article Snippet: For BioMark Fluidigm assays, the random hexamer generated cDNA was pre-amplified in multiplex specific-target-amplification (STA) reactions using TaqMan PreAmp master mix (Life Technologies) and with the following thermocycling conditions: 1 cycle at 95 ◦C for 10 min and 14 cycles of 95 ◦C for 15 s and 60 ◦C for 4 min. Preamplified cDNA was treated with exonuclease I (New England Biolabs) and diluted 5- (for BSF) or 10-fold (for PCF). .. High-throughput real-time PCR was then conducted on the BioMark HD system with Fluidigm 48-by-48 dynamic array integrated fluidic circuits (IFCs), using SsoFast EvaGreen supermix with Low ROX (Bio-Rad) and primers described in D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae699/7734165 by guest on 21 August 2024 Supplementary Table ST2 . .. Isolated RNA was treated with 10 U of Turbo DNase (Life Technologies) according to the manufacturer’s instructions and purified through acid phenol:chloroform extraction.

    Article Title: Biological hypoxia in pre-transplant human pancreatic islets induces transplant failure in diabetic mice
    Article Snippet: RNA was isolated from 500 IEQ of islets to synthesize cDNAs according to the manufacturer’s instructions (cDNA Synthesis Kit for RT-qPCR; Thermo Fisher Scientific, Waltham, MA). .. Microfluidic quantitative real-time PCR (qRT-PCR) was performed using the BioMark 48.48 Dynamic Array system (Standard Biotools, South San Francisco, CA). .. Genes previously demonstrating correlations to hypoxia, not limited to the pancreatic islet research, were chosen; TaqMan probes (Life Technologies, Carlsbad, CA) used in the qRT-PCR are listed in Table .

    Article Title: The gut microbiota and its metabolite butyrate shape metabolism and antiviral immunity along the gut-lung axis in the chicken
    Article Snippet: .. The qPCR employed primers, 2x SsoFast EvaGreen with Low Rox (BioRad 172-5211), and 20× DNA binding Dye (Fluidigm PN 100-7609) on a 48.48 Dynamic Array Integrated Fluidic Circuit (Fluidigm). ..

    Binding Assay:

    Article Title: The gut microbiota and its metabolite butyrate shape metabolism and antiviral immunity along the gut-lung axis in the chicken
    Article Snippet: .. The qPCR employed primers, 2x SsoFast EvaGreen with Low Rox (BioRad 172-5211), and 20× DNA binding Dye (Fluidigm PN 100-7609) on a 48.48 Dynamic Array Integrated Fluidic Circuit (Fluidigm). ..



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    Image Search Results


    a, Time-course analysis of E-cadherin and N-cadherin expression during neural tube formation. E- cadherin was initially broadly distributed but decreased in cells associated with the Matrigel region, whereas N-cadherin expression was initially weak and became apparent in cells on the Matrigel from day 6 onward. By day 10, the initially continuous monolayer had segregated into two distinct tissues corresponding to the surface ectoderm and neural tube. Scale bar, 50 μm. b, Three-dimensional imaging of tissues stained for N-cadherin, E-cadherin and cytokeratin 8. Section A–A′ shows an incompletely closed region, whereas section B–B′ shows a fully closed neural tube. Scale bar, 70 μm. c, Time-lapse imaging of actin dynamics from day 9 of culture (0 h) to day 10 (24 h). White arrows indicate actin-rich structures bridging the opposing tissue edges during closure. Scale bar, 30 μm. d, Myosin II distribution in the cell sheet during neural induction. Myosin II accumulated at the apical side of the cell sheet within the Matrigel-associated region. e, Distribution of SOX10 and ZIC1 before neural tube closure. Both markers were enriched at the boundary region between the neural and non- neural domains.

    Journal: bioRxiv

    Article Title: Engineering developmental transitions during human neurulation through modular integration of multimodal spatial cues

    doi: 10.64898/2026.10.07.757222

    Figure Lengend Snippet: a, Time-course analysis of E-cadherin and N-cadherin expression during neural tube formation. E- cadherin was initially broadly distributed but decreased in cells associated with the Matrigel region, whereas N-cadherin expression was initially weak and became apparent in cells on the Matrigel from day 6 onward. By day 10, the initially continuous monolayer had segregated into two distinct tissues corresponding to the surface ectoderm and neural tube. Scale bar, 50 μm. b, Three-dimensional imaging of tissues stained for N-cadherin, E-cadherin and cytokeratin 8. Section A–A′ shows an incompletely closed region, whereas section B–B′ shows a fully closed neural tube. Scale bar, 70 μm. c, Time-lapse imaging of actin dynamics from day 9 of culture (0 h) to day 10 (24 h). White arrows indicate actin-rich structures bridging the opposing tissue edges during closure. Scale bar, 30 μm. d, Myosin II distribution in the cell sheet during neural induction. Myosin II accumulated at the apical side of the cell sheet within the Matrigel-associated region. e, Distribution of SOX10 and ZIC1 before neural tube closure. Both markers were enriched at the boundary region between the neural and non- neural domains.

    Article Snippet: and Supplementary Video 4 show two-photon time-lapse imaging from day 9 to day 10, allowing visualization of actin dynamics by using SPY-actin (CY-SC202, Cytoskeleton Inc.) during surface ectoderm closure.

    Techniques: Analysis, Expressing, Imaging, Staining, Tissue