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dsu ix83 spinning disk microscope  (Olympus)


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    Structured Review

    Olympus dsu ix83 spinning disk microscope
    Dsu Ix83 Spinning Disk Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 5196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dsu+microscope/IX83+Inverted+Microscope+Currently+integrated+into+IXplore+Systems/pmc09668901-130-6-5
    Average 99 stars, based on 5196 article reviews
    dsu ix83 spinning disk microscope - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Ciprofloxacin-Induced Oxidative Stress Promotes a Senescence-like Phenotype in Human Fibroblasts In Vitro.
    Article Snippet: After washing with PBS, coverslips were mounted using Aqua-Poly/Mount (Polysciences, Warrington, PA, USA). .. Fluorescence microscopy was performed using an Olympus IX83 microscope (Olympus Corporation, Tokyo, Japan). ..

    Article Title: CDK4/6 Inhibitors and Curcumin Synergistically Suppress HER2- Breast Cancer via Lethal ROS and Inhibition of Metastasis.
    Article Snippet: Cells on coverslips were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton X- 100 (Biosharp, BS084), and blocked with 1% BSA. .. After incubation with primary antibodies overnight at 4°C, cells were treated with fluorescent secondary antibodies, stained with DAPI (Biosharp, BL739A), and imaged using an inverted fluorescence microscope (Olympus IX83, Japan). .. Cells were lysed with RIPA buffer (Beyotime, P0013B), and protein concentrations were measured using the BCA Protein Assay Kit (YEASEN, 20201ES76).

    Article Title: Melatonin Mitigates Bisphenol A- and MBP-Induced Damage in Leydig Cells by Alleviating Oxidative Stress and Endoplasmic Reticulum Stress via inhibition of PERK signaling pathway.
    Article Snippet: .. The cells were washed three times with serum-free DMEM/F12 medium and observed under an inverted fluorescence microscope (IX83, OLYMPUS), where cells expressing ROS exhibited Jo ur na l P re -p ro of green fluorescence. .. The average fluorescence intensity of ROS was analyzed using ImageJ software.

    Article Title: CAP1 mediates chronic inflammatory pain-induced anxiety through cAMP/PKA/NF-κB signaling pathway in the anterior cingulate cortex of male mice.
    Article Snippet: Please cite this article as: Jin, Y-c., Guo, Q-j., Yan, Y-x., Jiang, Y-l., Song, D-k., Lu, B-n., Zeng, C., Yue, J., Yang, L-k., Liu, R-x., Zhang, K., Li, X-b., he, R., Lu, Y-j., Wang, X-s., Liu, S-b., CAP1 mediates chronic inflammatory pain-induced anxiety through cAMP/PKA/NF-κB signaling pathway in the anterior cingulate cortex of male mice, Brain, Behavior, and Immunity (2026), doi: https://doi.org/10.1016/j.bbi.2026.107073

    Microscopy:

    Article Title: Ciprofloxacin-Induced Oxidative Stress Promotes a Senescence-like Phenotype in Human Fibroblasts In Vitro.
    Article Snippet: After washing with PBS, coverslips were mounted using Aqua-Poly/Mount (Polysciences, Warrington, PA, USA). .. Fluorescence microscopy was performed using an Olympus IX83 microscope (Olympus Corporation, Tokyo, Japan). ..

    Article Title: The isolated HEPN domain of SACSIN exhibits RNA-binding activity.
    Article Snippet: .. The expression of GFP-HEPN was acquired in live cells at the indicated time points, using the Evident-Olympus IX83 microscope. .. Protein extraction and Western Blot analysis Proteins were extracted using RIPA Buffer (10 mM Tris– HCl pH 8.0, 1% NP-40, 150 mM NaCl, 0.1% SDS, 0.1% NaDeoxycholate, 1 mM EDTA) complemented with cocktail protease inhibitor (11836153001, Merck Life Science).

    Article Title: CDK4/6 Inhibitors and Curcumin Synergistically Suppress HER2- Breast Cancer via Lethal ROS and Inhibition of Metastasis.
    Article Snippet: Cells on coverslips were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton X- 100 (Biosharp, BS084), and blocked with 1% BSA. .. After incubation with primary antibodies overnight at 4°C, cells were treated with fluorescent secondary antibodies, stained with DAPI (Biosharp, BL739A), and imaged using an inverted fluorescence microscope (Olympus IX83, Japan). .. Cells were lysed with RIPA buffer (Beyotime, P0013B), and protein concentrations were measured using the BCA Protein Assay Kit (YEASEN, 20201ES76).

    Article Title: Local control of Cre-regulated genetic modeling in vivo using wireless implantable optoelectronics.
    Article Snippet: .. At the end point of each experiment involving μ- ILED–induced local illumination (Fig. 3A), the 35- mm dishes of HEK293T cells in growth media (DMEM with 5% FBS and 1% glutamine) were transferred to the microscope imaging chamber under safe red lights and imaged using horizontal 2D area scanning using an Olympus IX83 microscope equipped with motorized Olympus SSU ultrasonic stage and the cellVivo Incubation System (Evident Scientific) that maintains the cells at 37°C and 5% CO2 during imaging. .. Imaging was performed with an EMCCD camera (Andor iXon Ultra 888; Oxford Instruments) paired with a 4X air objective (UPlanXApo 4X /0.16 NA).

    Article Title: Melatonin Mitigates Bisphenol A- and MBP-Induced Damage in Leydig Cells by Alleviating Oxidative Stress and Endoplasmic Reticulum Stress via inhibition of PERK signaling pathway.
    Article Snippet: .. The cells were washed three times with serum-free DMEM/F12 medium and observed under an inverted fluorescence microscope (IX83, OLYMPUS), where cells expressing ROS exhibited Jo ur na l P re -p ro of green fluorescence. .. The average fluorescence intensity of ROS was analyzed using ImageJ software.

    Expressing:

    Article Title: The isolated HEPN domain of SACSIN exhibits RNA-binding activity.
    Article Snippet: .. The expression of GFP-HEPN was acquired in live cells at the indicated time points, using the Evident-Olympus IX83 microscope. .. Protein extraction and Western Blot analysis Proteins were extracted using RIPA Buffer (10 mM Tris– HCl pH 8.0, 1% NP-40, 150 mM NaCl, 0.1% SDS, 0.1% NaDeoxycholate, 1 mM EDTA) complemented with cocktail protease inhibitor (11836153001, Merck Life Science).

    Article Title: Melatonin Mitigates Bisphenol A- and MBP-Induced Damage in Leydig Cells by Alleviating Oxidative Stress and Endoplasmic Reticulum Stress via inhibition of PERK signaling pathway.
    Article Snippet: .. The cells were washed three times with serum-free DMEM/F12 medium and observed under an inverted fluorescence microscope (IX83, OLYMPUS), where cells expressing ROS exhibited Jo ur na l P re -p ro of green fluorescence. .. The average fluorescence intensity of ROS was analyzed using ImageJ software.

    other:

    Article Title: An opioid efficacy switch for reversible optical control of analgesia.
    Article Snippet: In brief Azo-morphine-3 (AM-3) is a photoswitchable ligand that toggles between low and high efficacy states to control mu-opioid receptor activity in vitro and in vivo both in the brain and periphery.. AM-3 provides a powerful tool to probe the structural basis of opioid efficacy and enables spatiotemporally precise control of analgesia across pain models with reduced side effects compared with systemic drugs.

    Incubation:

    Article Title: CDK4/6 Inhibitors and Curcumin Synergistically Suppress HER2- Breast Cancer via Lethal ROS and Inhibition of Metastasis.
    Article Snippet: Cells on coverslips were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton X- 100 (Biosharp, BS084), and blocked with 1% BSA. .. After incubation with primary antibodies overnight at 4°C, cells were treated with fluorescent secondary antibodies, stained with DAPI (Biosharp, BL739A), and imaged using an inverted fluorescence microscope (Olympus IX83, Japan). .. Cells were lysed with RIPA buffer (Beyotime, P0013B), and protein concentrations were measured using the BCA Protein Assay Kit (YEASEN, 20201ES76).

    Article Title: Local control of Cre-regulated genetic modeling in vivo using wireless implantable optoelectronics.
    Article Snippet: .. At the end point of each experiment involving μ- ILED–induced local illumination (Fig. 3A), the 35- mm dishes of HEK293T cells in growth media (DMEM with 5% FBS and 1% glutamine) were transferred to the microscope imaging chamber under safe red lights and imaged using horizontal 2D area scanning using an Olympus IX83 microscope equipped with motorized Olympus SSU ultrasonic stage and the cellVivo Incubation System (Evident Scientific) that maintains the cells at 37°C and 5% CO2 during imaging. .. Imaging was performed with an EMCCD camera (Andor iXon Ultra 888; Oxford Instruments) paired with a 4X air objective (UPlanXApo 4X /0.16 NA).

    Staining:

    Article Title: CDK4/6 Inhibitors and Curcumin Synergistically Suppress HER2- Breast Cancer via Lethal ROS and Inhibition of Metastasis.
    Article Snippet: Cells on coverslips were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton X- 100 (Biosharp, BS084), and blocked with 1% BSA. .. After incubation with primary antibodies overnight at 4°C, cells were treated with fluorescent secondary antibodies, stained with DAPI (Biosharp, BL739A), and imaged using an inverted fluorescence microscope (Olympus IX83, Japan). .. Cells were lysed with RIPA buffer (Beyotime, P0013B), and protein concentrations were measured using the BCA Protein Assay Kit (YEASEN, 20201ES76).

    Imaging:

    Article Title: Local control of Cre-regulated genetic modeling in vivo using wireless implantable optoelectronics.
    Article Snippet: .. At the end point of each experiment involving μ- ILED–induced local illumination (Fig. 3A), the 35- mm dishes of HEK293T cells in growth media (DMEM with 5% FBS and 1% glutamine) were transferred to the microscope imaging chamber under safe red lights and imaged using horizontal 2D area scanning using an Olympus IX83 microscope equipped with motorized Olympus SSU ultrasonic stage and the cellVivo Incubation System (Evident Scientific) that maintains the cells at 37°C and 5% CO2 during imaging. .. Imaging was performed with an EMCCD camera (Andor iXon Ultra 888; Oxford Instruments) paired with a 4X air objective (UPlanXApo 4X /0.16 NA).

    Article Title: Transcriptomic reprogramming screen identifies SRSF1 as a regulator of aging.
    Article Snippet: The next day, the secondary antibody cocktail of antirabbit Alexa Fluor 488 (1:500; Thermo Fisher Scientific, catalog no. A-11008), antimouse Alexa Fluor 594 (1:500; Invitrogen, catalog no. A21203), Alexa Fluor 647-conjugated Phalloidin (1:500; Invitrogen, catalog no. A30107) and 4’,6-diamidino-2-phenylindole (1:5,000; Thermo Fisher Scientific, catalog no. 62249) was incubated for 1 h at RT. .. Cells were covered in ProLong Gold Antifade Mountant (Thermo Fisher Scientific, catalog no. P36930) before Z-stack imaging was captured using an Olympus IX83 laser scanning confocal. .. For ROS stress quantifications, live cells were first treated with H2O2 (200 μM, Thermo Fisher Scientific, catalog no. 426000250) for 6 h and washed with PBS.

    Inverted Microscopy:

    Article Title: CAP1 mediates chronic inflammatory pain-induced anxiety through cAMP/PKA/NF-κB signaling pathway in the anterior cingulate cortex of male mice.
    Article Snippet: Please cite this article as: Jin, Y-c., Guo, Q-j., Yan, Y-x., Jiang, Y-l., Song, D-k., Lu, B-n., Zeng, C., Yue, J., Yang, L-k., Liu, R-x., Zhang, K., Li, X-b., he, R., Lu, Y-j., Wang, X-s., Liu, S-b., CAP1 mediates chronic inflammatory pain-induced anxiety through cAMP/PKA/NF-κB signaling pathway in the anterior cingulate cortex of male mice, Brain, Behavior, and Immunity (2026), doi: https://doi.org/10.1016/j.bbi.2026.107073

    Confocal:

    Article Title: Transcriptomic reprogramming screen identifies SRSF1 as a regulator of aging.
    Article Snippet: The next day, the secondary antibody cocktail of antirabbit Alexa Fluor 488 (1:500; Thermo Fisher Scientific, catalog no. A-11008), antimouse Alexa Fluor 594 (1:500; Invitrogen, catalog no. A21203), Alexa Fluor 647-conjugated Phalloidin (1:500; Invitrogen, catalog no. A30107) and 4’,6-diamidino-2-phenylindole (1:5,000; Thermo Fisher Scientific, catalog no. 62249) was incubated for 1 h at RT. .. Cells were covered in ProLong Gold Antifade Mountant (Thermo Fisher Scientific, catalog no. P36930) before Z-stack imaging was captured using an Olympus IX83 laser scanning confocal. .. For ROS stress quantifications, live cells were first treated with H2O2 (200 μM, Thermo Fisher Scientific, catalog no. 426000250) for 6 h and washed with PBS.



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    5XFAD mice lacking KChIP3 (5XFAD/KChIP3 −/− ) increases microglia accumulation around βA plaques. Representative micrographs of βA plaques (Thioflavina-S, green) and microglia (Iba1, red) on ( a ) prefrontal cortex (CTX) and ( b ) dentate gyrus (DG) from WT, KChIP3 −/− , 5XFAD and 5XFAD/KChIP3 −/− . Magnification shows microglia accumulation around βA plaque on orthogonal views. Signal was captured using an Olympus FluoView 1000 inverted confocal multiphoton microscope. Scale bars: 100 μm <t>20X;</t> and 50 μm in magnification 150X (60X objective lens with 2.5 digital zoom). Data were analyzed by unpaired T-test (*** p < 0.0001). n = 100 plaques from 2 male mice of 11 months old
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    Image Search Results


    5XFAD mice lacking KChIP3 (5XFAD/KChIP3 −/− ) increases microglia accumulation around βA plaques. Representative micrographs of βA plaques (Thioflavina-S, green) and microglia (Iba1, red) on ( a ) prefrontal cortex (CTX) and ( b ) dentate gyrus (DG) from WT, KChIP3 −/− , 5XFAD and 5XFAD/KChIP3 −/− . Magnification shows microglia accumulation around βA plaque on orthogonal views. Signal was captured using an Olympus FluoView 1000 inverted confocal multiphoton microscope. Scale bars: 100 μm 20X; and 50 μm in magnification 150X (60X objective lens with 2.5 digital zoom). Data were analyzed by unpaired T-test (*** p < 0.0001). n = 100 plaques from 2 male mice of 11 months old

    Journal: Journal of Neuroinflammation

    Article Title: KChIP3 fosters neuroinflammation and synaptic dysfunction in the 5XFAD mouse model of Alzheimer’s disease

    doi: 10.1186/s12974-025-03426-2

    Figure Lengend Snippet: 5XFAD mice lacking KChIP3 (5XFAD/KChIP3 −/− ) increases microglia accumulation around βA plaques. Representative micrographs of βA plaques (Thioflavina-S, green) and microglia (Iba1, red) on ( a ) prefrontal cortex (CTX) and ( b ) dentate gyrus (DG) from WT, KChIP3 −/− , 5XFAD and 5XFAD/KChIP3 −/− . Magnification shows microglia accumulation around βA plaque on orthogonal views. Signal was captured using an Olympus FluoView 1000 inverted confocal multiphoton microscope. Scale bars: 100 μm 20X; and 50 μm in magnification 150X (60X objective lens with 2.5 digital zoom). Data were analyzed by unpaired T-test (*** p < 0.0001). n = 100 plaques from 2 male mice of 11 months old

    Article Snippet: 40 well-stained pyramidal neurons from the CA1 region of the dorsal hippocampus (bregma –2.03 to –2.27 mm), [ ] of each experimental group (ten neurons per mouse) were identified and captured in an Olympus BX51-WI-DSU (Disk Scanning Unit) microscope (Olympus, Tokyo, Japan; Objective Microscope: 20x) coupled to the Stereoinvestigator 9 R software (MBF Bioscience, Williston, VT, US).

    Techniques: Microscopy