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bovine serum albumin bsa  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bovine serum albumin bsa

    Bovine Serum Albumin Bsa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dsf+buffer/Bovine+Serum+Albumin/pmc09619376-260-7-11
    Average 99 stars, based on 1 article reviews
    bovine serum albumin bsa - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Ebola and Marburg virus VP35 coiled-coil validated as antiviral target by tripartite split-GFP complementation"

    Article Title: Ebola and Marburg virus VP35 coiled-coil validated as antiviral target by tripartite split-GFP complementation

    Journal: iScience

    doi: 10.1016/j.isci.2022.105354


    Figure Legend Snippet:

    Techniques Used: Virus, Recombinant, Protease Inhibitor, Software, Nano Differential Scanning Fluorimetry

    Related Articles

    Incubation:

    Article Title: BMP-7 mRNA delivered by Fibrin–CaP scaffolds activates osteogenic programs in vivo as evidenced by transcriptomic and proteomic analyses
    Article Snippet: Absorbance was measured at 562 nm using a CLARIOSTAR plate reader (BMG Labtech). .. Fixed cells were then incubated for 1 h at room temperature in a blocking solution containing 1% bovine serum albumin (BSA; Thermo Fisher Scientific), 10% normal goat serum (R&D Systems), 0.3 M glycine, and 0.1% Tween-20 in DPBS to minimize nonspecific binding. .. Following blocking, cells were incubated overnight at 4 °C with a primary rabbit anti-human osteopontin antibody (1:200; Abcam).

    Blocking Assay:

    Article Title: BMP-7 mRNA delivered by Fibrin–CaP scaffolds activates osteogenic programs in vivo as evidenced by transcriptomic and proteomic analyses
    Article Snippet: Absorbance was measured at 562 nm using a CLARIOSTAR plate reader (BMG Labtech). .. Fixed cells were then incubated for 1 h at room temperature in a blocking solution containing 1% bovine serum albumin (BSA; Thermo Fisher Scientific), 10% normal goat serum (R&D Systems), 0.3 M glycine, and 0.1% Tween-20 in DPBS to minimize nonspecific binding. .. Following blocking, cells were incubated overnight at 4 °C with a primary rabbit anti-human osteopontin antibody (1:200; Abcam).

    Binding Assay:

    Article Title: BMP-7 mRNA delivered by Fibrin–CaP scaffolds activates osteogenic programs in vivo as evidenced by transcriptomic and proteomic analyses
    Article Snippet: Absorbance was measured at 562 nm using a CLARIOSTAR plate reader (BMG Labtech). .. Fixed cells were then incubated for 1 h at room temperature in a blocking solution containing 1% bovine serum albumin (BSA; Thermo Fisher Scientific), 10% normal goat serum (R&D Systems), 0.3 M glycine, and 0.1% Tween-20 in DPBS to minimize nonspecific binding. .. Following blocking, cells were incubated overnight at 4 °C with a primary rabbit anti-human osteopontin antibody (1:200; Abcam).

    other:

    Article Title: Neonatal muscle-derived extracellular vesicles containing miR-542-3p rejuvenate aged skeletal muscle via a functional microneedle patch
    Article Snippet: Immunoreactive bands were visualized using an enhanced chemiluminescence (ECL) reagent (CST, USA), and band intensities were quantified with ImageJ software by normalizing the signal of the target protein to that of internal control.

    Article Title: Antioxidant and anti-inflammatory effects of the extract from rhizomes of Belamcanda chinensis (L.) DC. on human keratinocyte HaCaT cells
    Article Snippet: The cell line identity was verified by matching the supplier-provided STR profile with the HaCaT reference profile from Ubigene ( https://www.ubigene.us/ ).

    Article Title: Synergistic antibacterial effect of photocatalytic coatings activated by visible light and photodynamic therapy for rapid peri-implantitis treatment
    Article Snippet: HGF cells were also used to verify cell viability using the LIVE/DEAD Cell Imaging Kit (Invitrogen, Life Technologies), following the manufacturer's protocol.

    Labeling:

    Article Title: Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D
    Article Snippet: .. Briefly, gels were formed and left in a solution of 1 mg mL −1 of fluorescein isothiocyanate (FITC) labeled bovine serum albumin (BSA, Thermo Fisher) in PBS overnight at room temperature. .. A Leica STELLARIS 5 confocal microscope with HCX PL APO CS × 63.0/1.40 OIL objective was used to bleach a region of interest with a white light laser (WLL) for 20 s (until the region was fully bleached).

    Centrifugation:

    Article Title: Recombinant bone morphogenetic protein-2 attenuates colorectal cancer progression by orchestrating Hippo pathway activation, Yes-associated protein inhibition, and epithelial-mesenchymal transition suppression.
    Article Snippet: The cells were homogenized in Radioimmunoprecipitation Assay lysis buffer (G-Biosciences) containing protease and phosphatase inhibitors (Sigma) and incubated on ice for 30 min with gentle agitation. .. After centrifugation at 13,200 rpm for 30 min at 4°C, the supernatants were obtained, and protein concentrations were measured using a bovine serum albumin-based quantification kit (Thermo Fisher Scientific). .. Equal amounts of protein (30 μg) were separated using SDS-PAGE and transferred to polyvinylidene fluoride membranes (Amersham).

    Flow Cytometry:

    Article Title: 4D piezoceramic-integrated scaffolds with bioelectric cues for skeletal muscle regeneration
    Article Snippet: Protein content was quantified using a Lowry assay (DC protein assay kit, Bio-Rad), according to the manufacturer's instructions. .. Absorbance was measured at 750 nm using a microplate reader (Multiskan FC, Thermo Scientific), and protein concentrations were determined using a standard curve generated from known concentrations of bovine serum albumin (BSA, PAA). .. The porosity of the scaffolds was evaluated both qualitatively, using Scanning Electron Microscopy (SEM) (FEI Quanta 200, ESEM), and quantitatively, using X-ray micro-computed tomography (micro-CT).

    Standard Curve:

    Article Title: 4D piezoceramic-integrated scaffolds with bioelectric cues for skeletal muscle regeneration
    Article Snippet: Protein content was quantified using a Lowry assay (DC protein assay kit, Bio-Rad), according to the manufacturer's instructions. .. Absorbance was measured at 750 nm using a microplate reader (Multiskan FC, Thermo Scientific), and protein concentrations were determined using a standard curve generated from known concentrations of bovine serum albumin (BSA, PAA). .. The porosity of the scaffolds was evaluated both qualitatively, using Scanning Electron Microscopy (SEM) (FEI Quanta 200, ESEM), and quantitatively, using X-ray micro-computed tomography (micro-CT).

    Generated:

    Article Title: 4D piezoceramic-integrated scaffolds with bioelectric cues for skeletal muscle regeneration
    Article Snippet: Protein content was quantified using a Lowry assay (DC protein assay kit, Bio-Rad), according to the manufacturer's instructions. .. Absorbance was measured at 750 nm using a microplate reader (Multiskan FC, Thermo Scientific), and protein concentrations were determined using a standard curve generated from known concentrations of bovine serum albumin (BSA, PAA). .. The porosity of the scaffolds was evaluated both qualitatively, using Scanning Electron Microscopy (SEM) (FEI Quanta 200, ESEM), and quantitatively, using X-ray micro-computed tomography (micro-CT).



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    (a) UNC10088 C -induced conformational change of the loop adjacent to C326. Structural overlay of the SCF FBXO22 -UNC10088 C -NSD2 PWWP1 model (dark gray) and the SCF FBXO22 -BACH1 BTB complex (light gray, PDB: 8UA3) , revealing the displacement of FBXO22 residues Y390. (b) Chemical structures of UNC8732 analogs that contain an aromatic aldehyde in place of the aliphatic amine of UNC8732. (c) Benzaldehyde derivatives UNC10415667 and UNC10415668 promote FBXO22-dependent ubiquitination of *NSD2 PWWP1 when added at 0.5 µM, based on fluorescent scanning of an SDS-PAGE gel. (d) Treatment of U2OS NSD2-HiBit cells with UNC10415667 (1 µM) for 6 hours resulted in a potent reduction of NSD2 levels by about 75%. A more modest effect was observed upon treatment with UNC10415668, and all other compounds had no significant effect. n ≥ 3 independent experiments. Error bars: standard error of the mean. (e) Treatment of U2OS NSD2 HiBit cells with UNC10415667 in a dose response fashion revealed a DC 50 value of 0.46 µM, which is about 2-fold more potent than UNC8732 and UNC10088. n = 3 independent experiments. Error bars: standard error of the mean. (f) UNC10415667 uses C326 to recruit NSD2 to the FBXO22 FIST domain as monitored by size-exclusion chromatography. Chromatograms of different elution profiles between mixtures of WT FBXO22 FIST or a C326A mutant with NSD2 and UNC10415667 (top). Representative Coomassie-stained SDS-PAGE gels of collected fractions. (g) <t>Differential</t> <t>scanning</t> <t>fluorimetry</t> <t>(DSF)</t> was used to monitor the stability of FBXO22 FIST wild type and variants in the presence of UNC10088 or UNC10415667 compared to DMSO, revealing that both compounds stabilize WT FBXO22 FIST and the C326 single cysteine variant (C326 only) as evidenced by an increase in melting temperature (ΔT m ), but not the C326A mutant. n = 3 independent experiments. Error bars: standard error of the mean.
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    (a) UNC10088 C -induced conformational change of the loop adjacent to C326. Structural overlay of the SCF FBXO22 -UNC10088 C -NSD2 PWWP1 model (dark gray) and the SCF FBXO22 -BACH1 BTB complex (light gray, PDB: 8UA3) , revealing the displacement of FBXO22 residues Y390. (b) Chemical structures of UNC8732 analogs that contain an aromatic aldehyde in place of the aliphatic amine of UNC8732. (c) Benzaldehyde derivatives UNC10415667 and UNC10415668 promote FBXO22-dependent ubiquitination of *NSD2 PWWP1 when added at 0.5 µM, based on fluorescent scanning of an SDS-PAGE gel. (d) Treatment of U2OS NSD2-HiBit cells with UNC10415667 (1 µM) for 6 hours resulted in a potent reduction of NSD2 levels by about 75%. A more modest effect was observed upon treatment with UNC10415668, and all other compounds had no significant effect. n ≥ 3 independent experiments. Error bars: standard error of the mean. (e) Treatment of U2OS NSD2 HiBit cells with UNC10415667 in a dose response fashion revealed a DC 50 value of 0.46 µM, which is about 2-fold more potent than UNC8732 and UNC10088. n = 3 independent experiments. Error bars: standard error of the mean. (f) UNC10415667 uses C326 to recruit NSD2 to the FBXO22 FIST domain as monitored by size-exclusion chromatography. Chromatograms of different elution profiles between mixtures of WT FBXO22 FIST or a C326A mutant with NSD2 and UNC10415667 (top). Representative Coomassie-stained SDS-PAGE gels of collected fractions. (g) <t>Differential</t> <t>scanning</t> <t>fluorimetry</t> <t>(DSF)</t> was used to monitor the stability of FBXO22 FIST wild type and variants in the presence of UNC10088 or UNC10415667 compared to DMSO, revealing that both compounds stabilize WT FBXO22 FIST and the C326 single cysteine variant (C326 only) as evidenced by an increase in melting temperature (ΔT m ), but not the C326A mutant. n = 3 independent experiments. Error bars: standard error of the mean.
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    Image Search Results


    (a) UNC10088 C -induced conformational change of the loop adjacent to C326. Structural overlay of the SCF FBXO22 -UNC10088 C -NSD2 PWWP1 model (dark gray) and the SCF FBXO22 -BACH1 BTB complex (light gray, PDB: 8UA3) , revealing the displacement of FBXO22 residues Y390. (b) Chemical structures of UNC8732 analogs that contain an aromatic aldehyde in place of the aliphatic amine of UNC8732. (c) Benzaldehyde derivatives UNC10415667 and UNC10415668 promote FBXO22-dependent ubiquitination of *NSD2 PWWP1 when added at 0.5 µM, based on fluorescent scanning of an SDS-PAGE gel. (d) Treatment of U2OS NSD2-HiBit cells with UNC10415667 (1 µM) for 6 hours resulted in a potent reduction of NSD2 levels by about 75%. A more modest effect was observed upon treatment with UNC10415668, and all other compounds had no significant effect. n ≥ 3 independent experiments. Error bars: standard error of the mean. (e) Treatment of U2OS NSD2 HiBit cells with UNC10415667 in a dose response fashion revealed a DC 50 value of 0.46 µM, which is about 2-fold more potent than UNC8732 and UNC10088. n = 3 independent experiments. Error bars: standard error of the mean. (f) UNC10415667 uses C326 to recruit NSD2 to the FBXO22 FIST domain as monitored by size-exclusion chromatography. Chromatograms of different elution profiles between mixtures of WT FBXO22 FIST or a C326A mutant with NSD2 and UNC10415667 (top). Representative Coomassie-stained SDS-PAGE gels of collected fractions. (g) Differential scanning fluorimetry (DSF) was used to monitor the stability of FBXO22 FIST wild type and variants in the presence of UNC10088 or UNC10415667 compared to DMSO, revealing that both compounds stabilize WT FBXO22 FIST and the C326 single cysteine variant (C326 only) as evidenced by an increase in melting temperature (ΔT m ), but not the C326A mutant. n = 3 independent experiments. Error bars: standard error of the mean.

    Journal: bioRxiv

    Article Title: Structural basis of NSD2 degradation via targeted recruitment of SCF-FBXO22

    doi: 10.1101/2025.08.29.673087

    Figure Lengend Snippet: (a) UNC10088 C -induced conformational change of the loop adjacent to C326. Structural overlay of the SCF FBXO22 -UNC10088 C -NSD2 PWWP1 model (dark gray) and the SCF FBXO22 -BACH1 BTB complex (light gray, PDB: 8UA3) , revealing the displacement of FBXO22 residues Y390. (b) Chemical structures of UNC8732 analogs that contain an aromatic aldehyde in place of the aliphatic amine of UNC8732. (c) Benzaldehyde derivatives UNC10415667 and UNC10415668 promote FBXO22-dependent ubiquitination of *NSD2 PWWP1 when added at 0.5 µM, based on fluorescent scanning of an SDS-PAGE gel. (d) Treatment of U2OS NSD2-HiBit cells with UNC10415667 (1 µM) for 6 hours resulted in a potent reduction of NSD2 levels by about 75%. A more modest effect was observed upon treatment with UNC10415668, and all other compounds had no significant effect. n ≥ 3 independent experiments. Error bars: standard error of the mean. (e) Treatment of U2OS NSD2 HiBit cells with UNC10415667 in a dose response fashion revealed a DC 50 value of 0.46 µM, which is about 2-fold more potent than UNC8732 and UNC10088. n = 3 independent experiments. Error bars: standard error of the mean. (f) UNC10415667 uses C326 to recruit NSD2 to the FBXO22 FIST domain as monitored by size-exclusion chromatography. Chromatograms of different elution profiles between mixtures of WT FBXO22 FIST or a C326A mutant with NSD2 and UNC10415667 (top). Representative Coomassie-stained SDS-PAGE gels of collected fractions. (g) Differential scanning fluorimetry (DSF) was used to monitor the stability of FBXO22 FIST wild type and variants in the presence of UNC10088 or UNC10415667 compared to DMSO, revealing that both compounds stabilize WT FBXO22 FIST and the C326 single cysteine variant (C326 only) as evidenced by an increase in melting temperature (ΔT m ), but not the C326A mutant. n = 3 independent experiments. Error bars: standard error of the mean.

    Article Snippet: 9.5 μL of the master mix containing FBXO22 FIST WT or variants, SYPRO Orange Dye (Invitrogen), and DSF buffer (20 mM Tris 7.5, 150 mM NaCl, and 1 mM TCEP) were added to a 384-well qPCR plate (Genesee Scientific).

    Techniques: Ubiquitin Proteomics, SDS Page, Size-exclusion Chromatography, Mutagenesis, Staining, Variant Assay