Review



differential scanning fluorimetry dsf buffer  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher differential scanning fluorimetry dsf buffer
    Differential Scanning Fluorimetry Dsf Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dsf+buffer/TRIS-HCL/pmc04645622-260-20-46
    Average 99 stars, based on 1 article reviews
    differential scanning fluorimetry dsf buffer - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    SDS Page:

    Article Title: Usage of nanobody-beta-galactosidase fusion in immunoassays and its application in detecting a peanut allergen
    Article Snippet: Column purification was performed using an AKTA FPLC system (GE Healthcare). .. SDS-PAGE was performed using Invitrogen Bolt Bis-Tris Plus mini protein gels (4–12%) with a Bolt MES SDS running buffer (Fisher Scientific). .. The sample was filtered through 0.45 μm pore-sized filters and loaded onto a 5 mL HiTrap Phenyl FF column (GE Healthcare) pre-equilibrated with Buffer A containing 19.8% (w/v) of ammonium sulfate.

    Protein Concentration:

    Article Title: Comparative phosphoproteomic analysis reveals regulatory mechanisms underlying muscle fiber density in goslings
    Article Snippet: .. Muscle samples were lysed using SDT buffer (4% SDS, 100 mM Tris-HCl, pH 7.6, 0.1 M DTT), and protein concentration was determined using the BCA assay (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Title Pending 17760
    Article Snippet: This study investigated the effect of freezing and subsequent aging on beef quality, particularly focusing on the extent of postmortem proteolysis and tenderization.. The longissimus lumborum muscle was collected from 8 steers 24 h postmortem, sliced into 8 2.5-cm-thick steaks, and randomly allocated into 4 groups.. Treatment groups consisted of 1) aging at 4°C for 24 h; 2) aging for 168 h; 3) freezing at −20°C for 24 h followed by thawing/aging for 24 h; and 4) freezing for 24 h followed by thawing/aging for 168 h. In general, freezing decreased the color intensity of the steaks, whereas aging increased it (P< 0.05).

    BIA-KA:

    Article Title: Comparative phosphoproteomic analysis reveals regulatory mechanisms underlying muscle fiber density in goslings
    Article Snippet: .. Muscle samples were lysed using SDT buffer (4% SDS, 100 mM Tris-HCl, pH 7.6, 0.1 M DTT), and protein concentration was determined using the BCA assay (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Carbohydrate metabolism and phosphorylation cascades regulate flavor formation in chicken meat
    Article Snippet: .. Proteins were extracted in SDT buffer (4% SDS (Thermo Fisher Scientific), 100 mM Tris-HCl (Thermo Fisher Scientific), 1 mM DTT, pH 7.6) and quantified by BCA (Bio-Rad, Hercules, CA, USA). .. Proteins were digested by trypsin using the filter-aided sample preparation workflow, including cysteine alkylation with iodoacetamide in the dark, followed by desalting on C18 cartridges (Waters) and reconstitution in 0.1% formic acid.

    other:

    Article Title: Orthogonal characterization of rAAV reveals vector attributes that drive ITR repair, self-complementary genome formation, and transgene expression
    Article Snippet: SmaI restriction enzyme (20,000 units/mL; New England Biolabs) was used to digest rAAV genomes for 1 h at 37°C.

    Binding Assay:

    Article Title: H2A.Z facilitates Sox2-nucleosome interaction by promoting DNA and histone H3 tail mobility
    Article Snippet: Reactions were set up in 10 mM Tris–HCl pH 7.5, 100 mM KCl, 1 mM DTT, 0.2 mg/ml recombinant albumin (NEB), 0.02% Tween-20, 8% glycerol, containing 100 nM Lin28B DNA or NCP. .. Binding reactions were set up with or without Sox2 HMG (0.1 μM or 1 μM) and Oct4 CtoS (0.3 μM or 3 μM), and incubated at 25°C for 45 min. DNaseI (NEB) was added at 0.5 U (or 0.125 U) for NCP and 0.1 U (or 0.025 U) for DNA and incubated for 5 min at 25°C, inactivated by 40 μl quench buffer [10 mM Tris–HCl pH 7.5, 50 mM EDTA, 2% SDS, 300 ng/μl glycogen (Invitrogen)], and heating at 75°C for 30 min, as previously described [ ]. ..

    Incubation:

    Article Title: H2A.Z facilitates Sox2-nucleosome interaction by promoting DNA and histone H3 tail mobility
    Article Snippet: Reactions were set up in 10 mM Tris–HCl pH 7.5, 100 mM KCl, 1 mM DTT, 0.2 mg/ml recombinant albumin (NEB), 0.02% Tween-20, 8% glycerol, containing 100 nM Lin28B DNA or NCP. .. Binding reactions were set up with or without Sox2 HMG (0.1 μM or 1 μM) and Oct4 CtoS (0.3 μM or 3 μM), and incubated at 25°C for 45 min. DNaseI (NEB) was added at 0.5 U (or 0.125 U) for NCP and 0.1 U (or 0.025 U) for DNA and incubated for 5 min at 25°C, inactivated by 40 μl quench buffer [10 mM Tris–HCl pH 7.5, 50 mM EDTA, 2% SDS, 300 ng/μl glycogen (Invitrogen)], and heating at 75°C for 30 min, as previously described [ ]. ..

    Q-Buffer:

    Article Title: H2A.Z facilitates Sox2-nucleosome interaction by promoting DNA and histone H3 tail mobility
    Article Snippet: Reactions were set up in 10 mM Tris–HCl pH 7.5, 100 mM KCl, 1 mM DTT, 0.2 mg/ml recombinant albumin (NEB), 0.02% Tween-20, 8% glycerol, containing 100 nM Lin28B DNA or NCP. .. Binding reactions were set up with or without Sox2 HMG (0.1 μM or 1 μM) and Oct4 CtoS (0.3 μM or 3 μM), and incubated at 25°C for 45 min. DNaseI (NEB) was added at 0.5 U (or 0.125 U) for NCP and 0.1 U (or 0.025 U) for DNA and incubated for 5 min at 25°C, inactivated by 40 μl quench buffer [10 mM Tris–HCl pH 7.5, 50 mM EDTA, 2% SDS, 300 ng/μl glycogen (Invitrogen)], and heating at 75°C for 30 min, as previously described [ ]. ..

    Bicinchoninic Acid Protein Assay:

    Article Title: Title Pending 17760
    Article Snippet: This study investigated the effect of freezing and subsequent aging on beef quality, particularly focusing on the extent of postmortem proteolysis and tenderization.. The longissimus lumborum muscle was collected from 8 steers 24 h postmortem, sliced into 8 2.5-cm-thick steaks, and randomly allocated into 4 groups.. Treatment groups consisted of 1) aging at 4°C for 24 h; 2) aging for 168 h; 3) freezing at −20°C for 24 h followed by thawing/aging for 24 h; and 4) freezing for 24 h followed by thawing/aging for 168 h. In general, freezing decreased the color intensity of the steaks, whereas aging increased it (P< 0.05).



    Similar Products

    99
    Thermo Fisher dsf buffer
    Dsf Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dsf+buffer/Phosphate/pmc12657311-40-8-31
    Average 99 stars, based on 1 article reviews
    dsf buffer - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    93
    Genesee Scientific dsf buffer
    (a) UNC10088 C -induced conformational change of the loop adjacent to C326. Structural overlay of the SCF FBXO22 -UNC10088 C -NSD2 PWWP1 model (dark gray) and the SCF FBXO22 -BACH1 BTB complex (light gray, PDB: 8UA3) , revealing the displacement of FBXO22 residues Y390. (b) Chemical structures of UNC8732 analogs that contain an aromatic aldehyde in place of the aliphatic amine of UNC8732. (c) Benzaldehyde derivatives UNC10415667 and UNC10415668 promote FBXO22-dependent ubiquitination of *NSD2 PWWP1 when added at 0.5 µM, based on fluorescent scanning of an SDS-PAGE gel. (d) Treatment of U2OS NSD2-HiBit cells with UNC10415667 (1 µM) for 6 hours resulted in a potent reduction of NSD2 levels by about 75%. A more modest effect was observed upon treatment with UNC10415668, and all other compounds had no significant effect. n ≥ 3 independent experiments. Error bars: standard error of the mean. (e) Treatment of U2OS NSD2 HiBit cells with UNC10415667 in a dose response fashion revealed a DC 50 value of 0.46 µM, which is about 2-fold more potent than UNC8732 and UNC10088. n = 3 independent experiments. Error bars: standard error of the mean. (f) UNC10415667 uses C326 to recruit NSD2 to the FBXO22 FIST domain as monitored by size-exclusion chromatography. Chromatograms of different elution profiles between mixtures of WT FBXO22 FIST or a C326A mutant with NSD2 and UNC10415667 (top). Representative Coomassie-stained SDS-PAGE gels of collected fractions. (g) <t>Differential</t> <t>scanning</t> <t>fluorimetry</t> <t>(DSF)</t> was used to monitor the stability of FBXO22 FIST wild type and variants in the presence of UNC10088 or UNC10415667 compared to DMSO, revealing that both compounds stabilize WT FBXO22 FIST and the C326 single cysteine variant (C326 only) as evidenced by an increase in melting temperature (ΔT m ), but not the C326A mutant. n = 3 independent experiments. Error bars: standard error of the mean.
    Dsf Buffer, supplied by Genesee Scientific, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dsf+buffer/TCEP/bio_rxiv__2025__08__29__673087-262-17-37
    Average 93 stars, based on 1 article reviews
    dsf buffer - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    96
    Bio-Rad dsf buffer
    (a) UNC10088 C -induced conformational change of the loop adjacent to C326. Structural overlay of the SCF FBXO22 -UNC10088 C -NSD2 PWWP1 model (dark gray) and the SCF FBXO22 -BACH1 BTB complex (light gray, PDB: 8UA3) , revealing the displacement of FBXO22 residues Y390. (b) Chemical structures of UNC8732 analogs that contain an aromatic aldehyde in place of the aliphatic amine of UNC8732. (c) Benzaldehyde derivatives UNC10415667 and UNC10415668 promote FBXO22-dependent ubiquitination of *NSD2 PWWP1 when added at 0.5 µM, based on fluorescent scanning of an SDS-PAGE gel. (d) Treatment of U2OS NSD2-HiBit cells with UNC10415667 (1 µM) for 6 hours resulted in a potent reduction of NSD2 levels by about 75%. A more modest effect was observed upon treatment with UNC10415668, and all other compounds had no significant effect. n ≥ 3 independent experiments. Error bars: standard error of the mean. (e) Treatment of U2OS NSD2 HiBit cells with UNC10415667 in a dose response fashion revealed a DC 50 value of 0.46 µM, which is about 2-fold more potent than UNC8732 and UNC10088. n = 3 independent experiments. Error bars: standard error of the mean. (f) UNC10415667 uses C326 to recruit NSD2 to the FBXO22 FIST domain as monitored by size-exclusion chromatography. Chromatograms of different elution profiles between mixtures of WT FBXO22 FIST or a C326A mutant with NSD2 and UNC10415667 (top). Representative Coomassie-stained SDS-PAGE gels of collected fractions. (g) <t>Differential</t> <t>scanning</t> <t>fluorimetry</t> <t>(DSF)</t> was used to monitor the stability of FBXO22 FIST wild type and variants in the presence of UNC10088 or UNC10415667 compared to DMSO, revealing that both compounds stabilize WT FBXO22 FIST and the C326 single cysteine variant (C326 only) as evidenced by an increase in melting temperature (ΔT m ), but not the C326A mutant. n = 3 independent experiments. Error bars: standard error of the mean.
    Dsf Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dsf+buffer/Dithiothreitol+(DTT)/pmc12417823-39-11-32
    Average 96 stars, based on 1 article reviews
    dsf buffer - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    99
    Thermo Fisher 5x dsf buffer
    (a) UNC10088 C -induced conformational change of the loop adjacent to C326. Structural overlay of the SCF FBXO22 -UNC10088 C -NSD2 PWWP1 model (dark gray) and the SCF FBXO22 -BACH1 BTB complex (light gray, PDB: 8UA3) , revealing the displacement of FBXO22 residues Y390. (b) Chemical structures of UNC8732 analogs that contain an aromatic aldehyde in place of the aliphatic amine of UNC8732. (c) Benzaldehyde derivatives UNC10415667 and UNC10415668 promote FBXO22-dependent ubiquitination of *NSD2 PWWP1 when added at 0.5 µM, based on fluorescent scanning of an SDS-PAGE gel. (d) Treatment of U2OS NSD2-HiBit cells with UNC10415667 (1 µM) for 6 hours resulted in a potent reduction of NSD2 levels by about 75%. A more modest effect was observed upon treatment with UNC10415668, and all other compounds had no significant effect. n ≥ 3 independent experiments. Error bars: standard error of the mean. (e) Treatment of U2OS NSD2 HiBit cells with UNC10415667 in a dose response fashion revealed a DC 50 value of 0.46 µM, which is about 2-fold more potent than UNC8732 and UNC10088. n = 3 independent experiments. Error bars: standard error of the mean. (f) UNC10415667 uses C326 to recruit NSD2 to the FBXO22 FIST domain as monitored by size-exclusion chromatography. Chromatograms of different elution profiles between mixtures of WT FBXO22 FIST or a C326A mutant with NSD2 and UNC10415667 (top). Representative Coomassie-stained SDS-PAGE gels of collected fractions. (g) <t>Differential</t> <t>scanning</t> <t>fluorimetry</t> <t>(DSF)</t> was used to monitor the stability of FBXO22 FIST wild type and variants in the presence of UNC10088 or UNC10415667 compared to DMSO, revealing that both compounds stabilize WT FBXO22 FIST and the C326 single cysteine variant (C326 only) as evidenced by an increase in melting temperature (ΔT m ), but not the C326A mutant. n = 3 independent experiments. Error bars: standard error of the mean.
    5x Dsf Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dsf+buffer/HEPES/pm39662504-430-21-42
    Average 99 stars, based on 1 article reviews
    5x dsf buffer - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    Image Search Results


    (a) UNC10088 C -induced conformational change of the loop adjacent to C326. Structural overlay of the SCF FBXO22 -UNC10088 C -NSD2 PWWP1 model (dark gray) and the SCF FBXO22 -BACH1 BTB complex (light gray, PDB: 8UA3) , revealing the displacement of FBXO22 residues Y390. (b) Chemical structures of UNC8732 analogs that contain an aromatic aldehyde in place of the aliphatic amine of UNC8732. (c) Benzaldehyde derivatives UNC10415667 and UNC10415668 promote FBXO22-dependent ubiquitination of *NSD2 PWWP1 when added at 0.5 µM, based on fluorescent scanning of an SDS-PAGE gel. (d) Treatment of U2OS NSD2-HiBit cells with UNC10415667 (1 µM) for 6 hours resulted in a potent reduction of NSD2 levels by about 75%. A more modest effect was observed upon treatment with UNC10415668, and all other compounds had no significant effect. n ≥ 3 independent experiments. Error bars: standard error of the mean. (e) Treatment of U2OS NSD2 HiBit cells with UNC10415667 in a dose response fashion revealed a DC 50 value of 0.46 µM, which is about 2-fold more potent than UNC8732 and UNC10088. n = 3 independent experiments. Error bars: standard error of the mean. (f) UNC10415667 uses C326 to recruit NSD2 to the FBXO22 FIST domain as monitored by size-exclusion chromatography. Chromatograms of different elution profiles between mixtures of WT FBXO22 FIST or a C326A mutant with NSD2 and UNC10415667 (top). Representative Coomassie-stained SDS-PAGE gels of collected fractions. (g) Differential scanning fluorimetry (DSF) was used to monitor the stability of FBXO22 FIST wild type and variants in the presence of UNC10088 or UNC10415667 compared to DMSO, revealing that both compounds stabilize WT FBXO22 FIST and the C326 single cysteine variant (C326 only) as evidenced by an increase in melting temperature (ΔT m ), but not the C326A mutant. n = 3 independent experiments. Error bars: standard error of the mean.

    Journal: bioRxiv

    Article Title: Structural basis of NSD2 degradation via targeted recruitment of SCF-FBXO22

    doi: 10.1101/2025.08.29.673087

    Figure Lengend Snippet: (a) UNC10088 C -induced conformational change of the loop adjacent to C326. Structural overlay of the SCF FBXO22 -UNC10088 C -NSD2 PWWP1 model (dark gray) and the SCF FBXO22 -BACH1 BTB complex (light gray, PDB: 8UA3) , revealing the displacement of FBXO22 residues Y390. (b) Chemical structures of UNC8732 analogs that contain an aromatic aldehyde in place of the aliphatic amine of UNC8732. (c) Benzaldehyde derivatives UNC10415667 and UNC10415668 promote FBXO22-dependent ubiquitination of *NSD2 PWWP1 when added at 0.5 µM, based on fluorescent scanning of an SDS-PAGE gel. (d) Treatment of U2OS NSD2-HiBit cells with UNC10415667 (1 µM) for 6 hours resulted in a potent reduction of NSD2 levels by about 75%. A more modest effect was observed upon treatment with UNC10415668, and all other compounds had no significant effect. n ≥ 3 independent experiments. Error bars: standard error of the mean. (e) Treatment of U2OS NSD2 HiBit cells with UNC10415667 in a dose response fashion revealed a DC 50 value of 0.46 µM, which is about 2-fold more potent than UNC8732 and UNC10088. n = 3 independent experiments. Error bars: standard error of the mean. (f) UNC10415667 uses C326 to recruit NSD2 to the FBXO22 FIST domain as monitored by size-exclusion chromatography. Chromatograms of different elution profiles between mixtures of WT FBXO22 FIST or a C326A mutant with NSD2 and UNC10415667 (top). Representative Coomassie-stained SDS-PAGE gels of collected fractions. (g) Differential scanning fluorimetry (DSF) was used to monitor the stability of FBXO22 FIST wild type and variants in the presence of UNC10088 or UNC10415667 compared to DMSO, revealing that both compounds stabilize WT FBXO22 FIST and the C326 single cysteine variant (C326 only) as evidenced by an increase in melting temperature (ΔT m ), but not the C326A mutant. n = 3 independent experiments. Error bars: standard error of the mean.

    Article Snippet: 9.5 μL of the master mix containing FBXO22 FIST WT or variants, SYPRO Orange Dye (Invitrogen), and DSF buffer (20 mM Tris 7.5, 150 mM NaCl, and 1 mM TCEP) were added to a 384-well qPCR plate (Genesee Scientific).

    Techniques: Ubiquitin Proteomics, SDS Page, Size-exclusion Chromatography, Mutagenesis, Staining, Variant Assay