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dsf buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dsf buffer
    Dsf Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dsf+buffer/HEPES/pm35721509-84-5-22
    Average 99 stars, based on 1 article reviews
    dsf buffer - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Immunoprecipitation:

    Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies goat polyclonal α-GFP Rockland Cat#: 600-141-215; RRID: AB_1961516 rabbit polyclonal α-GFP Invitrogen Cat#: A11122; RRID: AB_221569 mouse monoclonal α-GAPDH SantaCruz Biotechnology Cat#: sc-32233; RRID: AB_627679 rabbit monoclonal α-HP1α Abcam Cat#: ab109028; RRID: AB_10858495 rabbit polyclonal α-H3K9me3 Abcam Cat#: 8898; RRID: AB_306848 mouse polyclonal α-H2A.X (Ser139) Millipore Cat#: 05-636; RRID: AB_309864 rabbit monoclonal α-H3 Abcam Cat#: ab176842; RRID: AB_2493104 rabbit polyclonal α-H3K9me3 Jenuwein lab # 1926 mouse monoclonal α-Nucleophosmin Abcam Cat#: ab10530; RRID: AB_297271 Chemicals, peptides, and recombinant proteins High-glucose Dulbecco’s Modified Eagle Medium (DMEM) Sigma-Aldrich Cat#: D6171 HyClone Characterized Fetal bovine serum Cytiva Cat#: SH30071.03 L-glutamine Sigma-Aldrich Cat#: G7513 β-mercaptoethanol Gibco Cat#: 31350-010 MEM Non-essential Amino Acid Solution (100×) Sigma-Aldrich Cat#: M7145 sodium pyruvate solution Sigma-Aldrich Cat#: S8636 penicillin-streptomycin Sigma-Aldrich Cat#: P4333 puromycin dihydrochloride Sigma-Aldrich Cat#: P9620 XhoI restriction enzyme New England Biolabs Cat#: R0146S AflII restriction enzyme New England Biolabs Cat#: R05020S PvuI restriction enzyme New England Biolabs Cat#: R3150S XFect Transfection reagent Takara Cat#: 631317 DPBS Gibco Cat#: 14190-094 RIPA Pierce Cat#: 89900 cOmplete EDTA-free protease inhibitors Roche Cat#: 11873580001 paraformaldehyde Santa Cruz Cat#: sc-281692 triton X-100 Sigma-Aldrich Cat#: X100 VECTASHIELD Antifade Mounting Medium with DAPI Vector Leboratories Cat#: H-1200 donkey serum Jackson ImmunoResearch Cat#: 017-000-121 phenol red-free DMEM Gibco Cat#: 21063029 1,6-hexanediol Sigma-Aldrich Cat#: 240117 low melting point agarose Bethesda Scientific Cat#: V3841 RNaseA Thermo Scientific Cat#: EN0531 mitoxantrone Selleckchem Cat#: S2485 cbl-0137 Selleckchem Cat#: S0507 formaldehyde Sigma-Aldrich Cat#: F8775 glycine Sigma-Aldrich Cat#: G8898 SDS Sigma-Aldrich Cat#: L3771 EDTA Sigma-Aldrich Cat#: 798681 tris-HCl Sigma-Aldrich Cat#: 108315 (Continued on next page) iScience 29, 115626, May 15, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NaCl Sigma-Aldrich Cat#: S5886 dynabeads Protein G for Immunoprecipitation Invitrogen Cat#: 10003D LiCl Merck Cat#: 310468 sodium deoxycholate Sigma-Aldrich Cat#: D6750 NaHCO3 Merck Cat#: S6297 proteinase K Thermo Scientific Cat#: EO0491 MNase Thermo Scientific Cat#: EN0181 SYBR Select Master Mix Applied Biosystems Cat#: 4472908 SYBR Safe DNA Gel Stain Invitrogen Cat#: S33102 HEPES Merck Cat#: H3375 MgCl2 Merck Cat#: M8266 CaCl2 Merck Cat#:449709 Critical commercial assays NucleoSpin Gel and PCR Clean-up Kit Macherey-Nagel Cat#: 740609.250 Deposited data Original western blot images Mendeley: https://doi.org/10.17632/ wccscb4vt3.1 N/A Experimental models: Cell lines W8 immortalized mouse embryonic fibroblasts Jenuwein lab W8 D5 immortalized mouse embryonic fibroblasts Jenuwein lab D5 NIH 3T3 HP1α-EGFP immortalized mouse embryonic fibroblasts Jenuwein lab N/A Oligonucleotides MSR_for (5’-TGGAATATGGC GAGAAAACTG) Ching et al.73 N/A MSR_rev (5’-AGGTCCTTCAG TGGGCATTT) Ching et al.73 N/A Recombinant DNA pCAGGS-Suv39h1-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #693 pCAGGS-Suv39h2-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #694 pCAGGS-Suv39h2ΔBD-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #703 pCAGGS-BD-Suv39h1-EGFP-IRES-Puro This study Laboratory of Thomas Jenuwein Database: #696 Software and algorithms R (v4.4.3) R Core Team https://www.r-project.org/ Clustal Omega EMBL-EBI https://www.ebi.ac.uk/Tools/msa/clustalo/ GraphPad Prism 10 GraphPad Software https://www.graphpad.com/ Peptides (v2.4.6) Charif & Lobry, 2007 https://cran.r-project.org/package=Peptides ggplot2 (v3.5.1) Hadley Wickham https://cran.r-project.org/package=ggplot2 ggrepel (v0.9.6) Slowikowski et al. https://cran.r-project.org/package=ggrepel IUPred2 Dosztányi et al., 2005 https://iupred2a.elte.hu/ Zen Black software Zeiss https://www.zeiss.com/microscopy/ int/products/microscope-software/zen.html Fiji (v2.9.0) Schindelin et al., 2012 https://fiji.sc/ Marvin JS (22.11.1) Chemaxon internal http://www.chemaxon.com e2 iScience 29, 115626, May 15, 2026 ..

    Staining:

    Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies goat polyclonal α-GFP Rockland Cat#: 600-141-215; RRID: AB_1961516 rabbit polyclonal α-GFP Invitrogen Cat#: A11122; RRID: AB_221569 mouse monoclonal α-GAPDH SantaCruz Biotechnology Cat#: sc-32233; RRID: AB_627679 rabbit monoclonal α-HP1α Abcam Cat#: ab109028; RRID: AB_10858495 rabbit polyclonal α-H3K9me3 Abcam Cat#: 8898; RRID: AB_306848 mouse polyclonal α-H2A.X (Ser139) Millipore Cat#: 05-636; RRID: AB_309864 rabbit monoclonal α-H3 Abcam Cat#: ab176842; RRID: AB_2493104 rabbit polyclonal α-H3K9me3 Jenuwein lab # 1926 mouse monoclonal α-Nucleophosmin Abcam Cat#: ab10530; RRID: AB_297271 Chemicals, peptides, and recombinant proteins High-glucose Dulbecco’s Modified Eagle Medium (DMEM) Sigma-Aldrich Cat#: D6171 HyClone Characterized Fetal bovine serum Cytiva Cat#: SH30071.03 L-glutamine Sigma-Aldrich Cat#: G7513 β-mercaptoethanol Gibco Cat#: 31350-010 MEM Non-essential Amino Acid Solution (100×) Sigma-Aldrich Cat#: M7145 sodium pyruvate solution Sigma-Aldrich Cat#: S8636 penicillin-streptomycin Sigma-Aldrich Cat#: P4333 puromycin dihydrochloride Sigma-Aldrich Cat#: P9620 XhoI restriction enzyme New England Biolabs Cat#: R0146S AflII restriction enzyme New England Biolabs Cat#: R05020S PvuI restriction enzyme New England Biolabs Cat#: R3150S XFect Transfection reagent Takara Cat#: 631317 DPBS Gibco Cat#: 14190-094 RIPA Pierce Cat#: 89900 cOmplete EDTA-free protease inhibitors Roche Cat#: 11873580001 paraformaldehyde Santa Cruz Cat#: sc-281692 triton X-100 Sigma-Aldrich Cat#: X100 VECTASHIELD Antifade Mounting Medium with DAPI Vector Leboratories Cat#: H-1200 donkey serum Jackson ImmunoResearch Cat#: 017-000-121 phenol red-free DMEM Gibco Cat#: 21063029 1,6-hexanediol Sigma-Aldrich Cat#: 240117 low melting point agarose Bethesda Scientific Cat#: V3841 RNaseA Thermo Scientific Cat#: EN0531 mitoxantrone Selleckchem Cat#: S2485 cbl-0137 Selleckchem Cat#: S0507 formaldehyde Sigma-Aldrich Cat#: F8775 glycine Sigma-Aldrich Cat#: G8898 SDS Sigma-Aldrich Cat#: L3771 EDTA Sigma-Aldrich Cat#: 798681 tris-HCl Sigma-Aldrich Cat#: 108315 (Continued on next page) iScience 29, 115626, May 15, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NaCl Sigma-Aldrich Cat#: S5886 dynabeads Protein G for Immunoprecipitation Invitrogen Cat#: 10003D LiCl Merck Cat#: 310468 sodium deoxycholate Sigma-Aldrich Cat#: D6750 NaHCO3 Merck Cat#: S6297 proteinase K Thermo Scientific Cat#: EO0491 MNase Thermo Scientific Cat#: EN0181 SYBR Select Master Mix Applied Biosystems Cat#: 4472908 SYBR Safe DNA Gel Stain Invitrogen Cat#: S33102 HEPES Merck Cat#: H3375 MgCl2 Merck Cat#: M8266 CaCl2 Merck Cat#:449709 Critical commercial assays NucleoSpin Gel and PCR Clean-up Kit Macherey-Nagel Cat#: 740609.250 Deposited data Original western blot images Mendeley: https://doi.org/10.17632/ wccscb4vt3.1 N/A Experimental models: Cell lines W8 immortalized mouse embryonic fibroblasts Jenuwein lab W8 D5 immortalized mouse embryonic fibroblasts Jenuwein lab D5 NIH 3T3 HP1α-EGFP immortalized mouse embryonic fibroblasts Jenuwein lab N/A Oligonucleotides MSR_for (5’-TGGAATATGGC GAGAAAACTG) Ching et al.73 N/A MSR_rev (5’-AGGTCCTTCAG TGGGCATTT) Ching et al.73 N/A Recombinant DNA pCAGGS-Suv39h1-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #693 pCAGGS-Suv39h2-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #694 pCAGGS-Suv39h2ΔBD-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #703 pCAGGS-BD-Suv39h1-EGFP-IRES-Puro This study Laboratory of Thomas Jenuwein Database: #696 Software and algorithms R (v4.4.3) R Core Team https://www.r-project.org/ Clustal Omega EMBL-EBI https://www.ebi.ac.uk/Tools/msa/clustalo/ GraphPad Prism 10 GraphPad Software https://www.graphpad.com/ Peptides (v2.4.6) Charif & Lobry, 2007 https://cran.r-project.org/package=Peptides ggplot2 (v3.5.1) Hadley Wickham https://cran.r-project.org/package=ggplot2 ggrepel (v0.9.6) Slowikowski et al. https://cran.r-project.org/package=ggrepel IUPred2 Dosztányi et al., 2005 https://iupred2a.elte.hu/ Zen Black software Zeiss https://www.zeiss.com/microscopy/ int/products/microscope-software/zen.html Fiji (v2.9.0) Schindelin et al., 2012 https://fiji.sc/ Marvin JS (22.11.1) Chemaxon internal http://www.chemaxon.com e2 iScience 29, 115626, May 15, 2026 ..

    Article Title: T-tubular remodeling occurs in a cardiomyocyte-autonomous manner following pathological mechanical stiffness exposure
    Article Snippet: .. Isolated cardiomyocytes were plated onto 10 μg/ml laminin-coated surfaces and maintained in M199 (ThermoFisher Scientific) supplemented with 1× insulin-transferrinselenium-X (Sigma), 1 μg/μl primocin (InvivoGen), and 20 mM HEPES (ThermoFisher Scientific), pH = 7.4, cultured at 37 °C and 5% CO 2 with the addition of 25 μmol/L of cytochalasin D (Cayman) in the media for 48 h. 10 μg/ml Hoechst 33258 (Invitrogen, H3569), a DNA-binding dye was utilized to stain cardiomyocytes nuclei. ..

    Polymerase Chain Reaction:

    Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies goat polyclonal α-GFP Rockland Cat#: 600-141-215; RRID: AB_1961516 rabbit polyclonal α-GFP Invitrogen Cat#: A11122; RRID: AB_221569 mouse monoclonal α-GAPDH SantaCruz Biotechnology Cat#: sc-32233; RRID: AB_627679 rabbit monoclonal α-HP1α Abcam Cat#: ab109028; RRID: AB_10858495 rabbit polyclonal α-H3K9me3 Abcam Cat#: 8898; RRID: AB_306848 mouse polyclonal α-H2A.X (Ser139) Millipore Cat#: 05-636; RRID: AB_309864 rabbit monoclonal α-H3 Abcam Cat#: ab176842; RRID: AB_2493104 rabbit polyclonal α-H3K9me3 Jenuwein lab # 1926 mouse monoclonal α-Nucleophosmin Abcam Cat#: ab10530; RRID: AB_297271 Chemicals, peptides, and recombinant proteins High-glucose Dulbecco’s Modified Eagle Medium (DMEM) Sigma-Aldrich Cat#: D6171 HyClone Characterized Fetal bovine serum Cytiva Cat#: SH30071.03 L-glutamine Sigma-Aldrich Cat#: G7513 β-mercaptoethanol Gibco Cat#: 31350-010 MEM Non-essential Amino Acid Solution (100×) Sigma-Aldrich Cat#: M7145 sodium pyruvate solution Sigma-Aldrich Cat#: S8636 penicillin-streptomycin Sigma-Aldrich Cat#: P4333 puromycin dihydrochloride Sigma-Aldrich Cat#: P9620 XhoI restriction enzyme New England Biolabs Cat#: R0146S AflII restriction enzyme New England Biolabs Cat#: R05020S PvuI restriction enzyme New England Biolabs Cat#: R3150S XFect Transfection reagent Takara Cat#: 631317 DPBS Gibco Cat#: 14190-094 RIPA Pierce Cat#: 89900 cOmplete EDTA-free protease inhibitors Roche Cat#: 11873580001 paraformaldehyde Santa Cruz Cat#: sc-281692 triton X-100 Sigma-Aldrich Cat#: X100 VECTASHIELD Antifade Mounting Medium with DAPI Vector Leboratories Cat#: H-1200 donkey serum Jackson ImmunoResearch Cat#: 017-000-121 phenol red-free DMEM Gibco Cat#: 21063029 1,6-hexanediol Sigma-Aldrich Cat#: 240117 low melting point agarose Bethesda Scientific Cat#: V3841 RNaseA Thermo Scientific Cat#: EN0531 mitoxantrone Selleckchem Cat#: S2485 cbl-0137 Selleckchem Cat#: S0507 formaldehyde Sigma-Aldrich Cat#: F8775 glycine Sigma-Aldrich Cat#: G8898 SDS Sigma-Aldrich Cat#: L3771 EDTA Sigma-Aldrich Cat#: 798681 tris-HCl Sigma-Aldrich Cat#: 108315 (Continued on next page) iScience 29, 115626, May 15, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NaCl Sigma-Aldrich Cat#: S5886 dynabeads Protein G for Immunoprecipitation Invitrogen Cat#: 10003D LiCl Merck Cat#: 310468 sodium deoxycholate Sigma-Aldrich Cat#: D6750 NaHCO3 Merck Cat#: S6297 proteinase K Thermo Scientific Cat#: EO0491 MNase Thermo Scientific Cat#: EN0181 SYBR Select Master Mix Applied Biosystems Cat#: 4472908 SYBR Safe DNA Gel Stain Invitrogen Cat#: S33102 HEPES Merck Cat#: H3375 MgCl2 Merck Cat#: M8266 CaCl2 Merck Cat#:449709 Critical commercial assays NucleoSpin Gel and PCR Clean-up Kit Macherey-Nagel Cat#: 740609.250 Deposited data Original western blot images Mendeley: https://doi.org/10.17632/ wccscb4vt3.1 N/A Experimental models: Cell lines W8 immortalized mouse embryonic fibroblasts Jenuwein lab W8 D5 immortalized mouse embryonic fibroblasts Jenuwein lab D5 NIH 3T3 HP1α-EGFP immortalized mouse embryonic fibroblasts Jenuwein lab N/A Oligonucleotides MSR_for (5’-TGGAATATGGC GAGAAAACTG) Ching et al.73 N/A MSR_rev (5’-AGGTCCTTCAG TGGGCATTT) Ching et al.73 N/A Recombinant DNA pCAGGS-Suv39h1-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #693 pCAGGS-Suv39h2-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #694 pCAGGS-Suv39h2ΔBD-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #703 pCAGGS-BD-Suv39h1-EGFP-IRES-Puro This study Laboratory of Thomas Jenuwein Database: #696 Software and algorithms R (v4.4.3) R Core Team https://www.r-project.org/ Clustal Omega EMBL-EBI https://www.ebi.ac.uk/Tools/msa/clustalo/ GraphPad Prism 10 GraphPad Software https://www.graphpad.com/ Peptides (v2.4.6) Charif & Lobry, 2007 https://cran.r-project.org/package=Peptides ggplot2 (v3.5.1) Hadley Wickham https://cran.r-project.org/package=ggplot2 ggrepel (v0.9.6) Slowikowski et al. https://cran.r-project.org/package=ggrepel IUPred2 Dosztányi et al., 2005 https://iupred2a.elte.hu/ Zen Black software Zeiss https://www.zeiss.com/microscopy/ int/products/microscope-software/zen.html Fiji (v2.9.0) Schindelin et al., 2012 https://fiji.sc/ Marvin JS (22.11.1) Chemaxon internal http://www.chemaxon.com e2 iScience 29, 115626, May 15, 2026 ..

    Western Blot:

    Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies goat polyclonal α-GFP Rockland Cat#: 600-141-215; RRID: AB_1961516 rabbit polyclonal α-GFP Invitrogen Cat#: A11122; RRID: AB_221569 mouse monoclonal α-GAPDH SantaCruz Biotechnology Cat#: sc-32233; RRID: AB_627679 rabbit monoclonal α-HP1α Abcam Cat#: ab109028; RRID: AB_10858495 rabbit polyclonal α-H3K9me3 Abcam Cat#: 8898; RRID: AB_306848 mouse polyclonal α-H2A.X (Ser139) Millipore Cat#: 05-636; RRID: AB_309864 rabbit monoclonal α-H3 Abcam Cat#: ab176842; RRID: AB_2493104 rabbit polyclonal α-H3K9me3 Jenuwein lab # 1926 mouse monoclonal α-Nucleophosmin Abcam Cat#: ab10530; RRID: AB_297271 Chemicals, peptides, and recombinant proteins High-glucose Dulbecco’s Modified Eagle Medium (DMEM) Sigma-Aldrich Cat#: D6171 HyClone Characterized Fetal bovine serum Cytiva Cat#: SH30071.03 L-glutamine Sigma-Aldrich Cat#: G7513 β-mercaptoethanol Gibco Cat#: 31350-010 MEM Non-essential Amino Acid Solution (100×) Sigma-Aldrich Cat#: M7145 sodium pyruvate solution Sigma-Aldrich Cat#: S8636 penicillin-streptomycin Sigma-Aldrich Cat#: P4333 puromycin dihydrochloride Sigma-Aldrich Cat#: P9620 XhoI restriction enzyme New England Biolabs Cat#: R0146S AflII restriction enzyme New England Biolabs Cat#: R05020S PvuI restriction enzyme New England Biolabs Cat#: R3150S XFect Transfection reagent Takara Cat#: 631317 DPBS Gibco Cat#: 14190-094 RIPA Pierce Cat#: 89900 cOmplete EDTA-free protease inhibitors Roche Cat#: 11873580001 paraformaldehyde Santa Cruz Cat#: sc-281692 triton X-100 Sigma-Aldrich Cat#: X100 VECTASHIELD Antifade Mounting Medium with DAPI Vector Leboratories Cat#: H-1200 donkey serum Jackson ImmunoResearch Cat#: 017-000-121 phenol red-free DMEM Gibco Cat#: 21063029 1,6-hexanediol Sigma-Aldrich Cat#: 240117 low melting point agarose Bethesda Scientific Cat#: V3841 RNaseA Thermo Scientific Cat#: EN0531 mitoxantrone Selleckchem Cat#: S2485 cbl-0137 Selleckchem Cat#: S0507 formaldehyde Sigma-Aldrich Cat#: F8775 glycine Sigma-Aldrich Cat#: G8898 SDS Sigma-Aldrich Cat#: L3771 EDTA Sigma-Aldrich Cat#: 798681 tris-HCl Sigma-Aldrich Cat#: 108315 (Continued on next page) iScience 29, 115626, May 15, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NaCl Sigma-Aldrich Cat#: S5886 dynabeads Protein G for Immunoprecipitation Invitrogen Cat#: 10003D LiCl Merck Cat#: 310468 sodium deoxycholate Sigma-Aldrich Cat#: D6750 NaHCO3 Merck Cat#: S6297 proteinase K Thermo Scientific Cat#: EO0491 MNase Thermo Scientific Cat#: EN0181 SYBR Select Master Mix Applied Biosystems Cat#: 4472908 SYBR Safe DNA Gel Stain Invitrogen Cat#: S33102 HEPES Merck Cat#: H3375 MgCl2 Merck Cat#: M8266 CaCl2 Merck Cat#:449709 Critical commercial assays NucleoSpin Gel and PCR Clean-up Kit Macherey-Nagel Cat#: 740609.250 Deposited data Original western blot images Mendeley: https://doi.org/10.17632/ wccscb4vt3.1 N/A Experimental models: Cell lines W8 immortalized mouse embryonic fibroblasts Jenuwein lab W8 D5 immortalized mouse embryonic fibroblasts Jenuwein lab D5 NIH 3T3 HP1α-EGFP immortalized mouse embryonic fibroblasts Jenuwein lab N/A Oligonucleotides MSR_for (5’-TGGAATATGGC GAGAAAACTG) Ching et al.73 N/A MSR_rev (5’-AGGTCCTTCAG TGGGCATTT) Ching et al.73 N/A Recombinant DNA pCAGGS-Suv39h1-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #693 pCAGGS-Suv39h2-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #694 pCAGGS-Suv39h2ΔBD-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #703 pCAGGS-BD-Suv39h1-EGFP-IRES-Puro This study Laboratory of Thomas Jenuwein Database: #696 Software and algorithms R (v4.4.3) R Core Team https://www.r-project.org/ Clustal Omega EMBL-EBI https://www.ebi.ac.uk/Tools/msa/clustalo/ GraphPad Prism 10 GraphPad Software https://www.graphpad.com/ Peptides (v2.4.6) Charif & Lobry, 2007 https://cran.r-project.org/package=Peptides ggplot2 (v3.5.1) Hadley Wickham https://cran.r-project.org/package=ggplot2 ggrepel (v0.9.6) Slowikowski et al. https://cran.r-project.org/package=ggrepel IUPred2 Dosztányi et al., 2005 https://iupred2a.elte.hu/ Zen Black software Zeiss https://www.zeiss.com/microscopy/ int/products/microscope-software/zen.html Fiji (v2.9.0) Schindelin et al., 2012 https://fiji.sc/ Marvin JS (22.11.1) Chemaxon internal http://www.chemaxon.com e2 iScience 29, 115626, May 15, 2026 ..

    Recombinant:

    Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies goat polyclonal α-GFP Rockland Cat#: 600-141-215; RRID: AB_1961516 rabbit polyclonal α-GFP Invitrogen Cat#: A11122; RRID: AB_221569 mouse monoclonal α-GAPDH SantaCruz Biotechnology Cat#: sc-32233; RRID: AB_627679 rabbit monoclonal α-HP1α Abcam Cat#: ab109028; RRID: AB_10858495 rabbit polyclonal α-H3K9me3 Abcam Cat#: 8898; RRID: AB_306848 mouse polyclonal α-H2A.X (Ser139) Millipore Cat#: 05-636; RRID: AB_309864 rabbit monoclonal α-H3 Abcam Cat#: ab176842; RRID: AB_2493104 rabbit polyclonal α-H3K9me3 Jenuwein lab # 1926 mouse monoclonal α-Nucleophosmin Abcam Cat#: ab10530; RRID: AB_297271 Chemicals, peptides, and recombinant proteins High-glucose Dulbecco’s Modified Eagle Medium (DMEM) Sigma-Aldrich Cat#: D6171 HyClone Characterized Fetal bovine serum Cytiva Cat#: SH30071.03 L-glutamine Sigma-Aldrich Cat#: G7513 β-mercaptoethanol Gibco Cat#: 31350-010 MEM Non-essential Amino Acid Solution (100×) Sigma-Aldrich Cat#: M7145 sodium pyruvate solution Sigma-Aldrich Cat#: S8636 penicillin-streptomycin Sigma-Aldrich Cat#: P4333 puromycin dihydrochloride Sigma-Aldrich Cat#: P9620 XhoI restriction enzyme New England Biolabs Cat#: R0146S AflII restriction enzyme New England Biolabs Cat#: R05020S PvuI restriction enzyme New England Biolabs Cat#: R3150S XFect Transfection reagent Takara Cat#: 631317 DPBS Gibco Cat#: 14190-094 RIPA Pierce Cat#: 89900 cOmplete EDTA-free protease inhibitors Roche Cat#: 11873580001 paraformaldehyde Santa Cruz Cat#: sc-281692 triton X-100 Sigma-Aldrich Cat#: X100 VECTASHIELD Antifade Mounting Medium with DAPI Vector Leboratories Cat#: H-1200 donkey serum Jackson ImmunoResearch Cat#: 017-000-121 phenol red-free DMEM Gibco Cat#: 21063029 1,6-hexanediol Sigma-Aldrich Cat#: 240117 low melting point agarose Bethesda Scientific Cat#: V3841 RNaseA Thermo Scientific Cat#: EN0531 mitoxantrone Selleckchem Cat#: S2485 cbl-0137 Selleckchem Cat#: S0507 formaldehyde Sigma-Aldrich Cat#: F8775 glycine Sigma-Aldrich Cat#: G8898 SDS Sigma-Aldrich Cat#: L3771 EDTA Sigma-Aldrich Cat#: 798681 tris-HCl Sigma-Aldrich Cat#: 108315 (Continued on next page) iScience 29, 115626, May 15, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NaCl Sigma-Aldrich Cat#: S5886 dynabeads Protein G for Immunoprecipitation Invitrogen Cat#: 10003D LiCl Merck Cat#: 310468 sodium deoxycholate Sigma-Aldrich Cat#: D6750 NaHCO3 Merck Cat#: S6297 proteinase K Thermo Scientific Cat#: EO0491 MNase Thermo Scientific Cat#: EN0181 SYBR Select Master Mix Applied Biosystems Cat#: 4472908 SYBR Safe DNA Gel Stain Invitrogen Cat#: S33102 HEPES Merck Cat#: H3375 MgCl2 Merck Cat#: M8266 CaCl2 Merck Cat#:449709 Critical commercial assays NucleoSpin Gel and PCR Clean-up Kit Macherey-Nagel Cat#: 740609.250 Deposited data Original western blot images Mendeley: https://doi.org/10.17632/ wccscb4vt3.1 N/A Experimental models: Cell lines W8 immortalized mouse embryonic fibroblasts Jenuwein lab W8 D5 immortalized mouse embryonic fibroblasts Jenuwein lab D5 NIH 3T3 HP1α-EGFP immortalized mouse embryonic fibroblasts Jenuwein lab N/A Oligonucleotides MSR_for (5’-TGGAATATGGC GAGAAAACTG) Ching et al.73 N/A MSR_rev (5’-AGGTCCTTCAG TGGGCATTT) Ching et al.73 N/A Recombinant DNA pCAGGS-Suv39h1-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #693 pCAGGS-Suv39h2-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #694 pCAGGS-Suv39h2ΔBD-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #703 pCAGGS-BD-Suv39h1-EGFP-IRES-Puro This study Laboratory of Thomas Jenuwein Database: #696 Software and algorithms R (v4.4.3) R Core Team https://www.r-project.org/ Clustal Omega EMBL-EBI https://www.ebi.ac.uk/Tools/msa/clustalo/ GraphPad Prism 10 GraphPad Software https://www.graphpad.com/ Peptides (v2.4.6) Charif & Lobry, 2007 https://cran.r-project.org/package=Peptides ggplot2 (v3.5.1) Hadley Wickham https://cran.r-project.org/package=ggplot2 ggrepel (v0.9.6) Slowikowski et al. https://cran.r-project.org/package=ggrepel IUPred2 Dosztányi et al., 2005 https://iupred2a.elte.hu/ Zen Black software Zeiss https://www.zeiss.com/microscopy/ int/products/microscope-software/zen.html Fiji (v2.9.0) Schindelin et al., 2012 https://fiji.sc/ Marvin JS (22.11.1) Chemaxon internal http://www.chemaxon.com e2 iScience 29, 115626, May 15, 2026 ..

    Software:

    Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies goat polyclonal α-GFP Rockland Cat#: 600-141-215; RRID: AB_1961516 rabbit polyclonal α-GFP Invitrogen Cat#: A11122; RRID: AB_221569 mouse monoclonal α-GAPDH SantaCruz Biotechnology Cat#: sc-32233; RRID: AB_627679 rabbit monoclonal α-HP1α Abcam Cat#: ab109028; RRID: AB_10858495 rabbit polyclonal α-H3K9me3 Abcam Cat#: 8898; RRID: AB_306848 mouse polyclonal α-H2A.X (Ser139) Millipore Cat#: 05-636; RRID: AB_309864 rabbit monoclonal α-H3 Abcam Cat#: ab176842; RRID: AB_2493104 rabbit polyclonal α-H3K9me3 Jenuwein lab # 1926 mouse monoclonal α-Nucleophosmin Abcam Cat#: ab10530; RRID: AB_297271 Chemicals, peptides, and recombinant proteins High-glucose Dulbecco’s Modified Eagle Medium (DMEM) Sigma-Aldrich Cat#: D6171 HyClone Characterized Fetal bovine serum Cytiva Cat#: SH30071.03 L-glutamine Sigma-Aldrich Cat#: G7513 β-mercaptoethanol Gibco Cat#: 31350-010 MEM Non-essential Amino Acid Solution (100×) Sigma-Aldrich Cat#: M7145 sodium pyruvate solution Sigma-Aldrich Cat#: S8636 penicillin-streptomycin Sigma-Aldrich Cat#: P4333 puromycin dihydrochloride Sigma-Aldrich Cat#: P9620 XhoI restriction enzyme New England Biolabs Cat#: R0146S AflII restriction enzyme New England Biolabs Cat#: R05020S PvuI restriction enzyme New England Biolabs Cat#: R3150S XFect Transfection reagent Takara Cat#: 631317 DPBS Gibco Cat#: 14190-094 RIPA Pierce Cat#: 89900 cOmplete EDTA-free protease inhibitors Roche Cat#: 11873580001 paraformaldehyde Santa Cruz Cat#: sc-281692 triton X-100 Sigma-Aldrich Cat#: X100 VECTASHIELD Antifade Mounting Medium with DAPI Vector Leboratories Cat#: H-1200 donkey serum Jackson ImmunoResearch Cat#: 017-000-121 phenol red-free DMEM Gibco Cat#: 21063029 1,6-hexanediol Sigma-Aldrich Cat#: 240117 low melting point agarose Bethesda Scientific Cat#: V3841 RNaseA Thermo Scientific Cat#: EN0531 mitoxantrone Selleckchem Cat#: S2485 cbl-0137 Selleckchem Cat#: S0507 formaldehyde Sigma-Aldrich Cat#: F8775 glycine Sigma-Aldrich Cat#: G8898 SDS Sigma-Aldrich Cat#: L3771 EDTA Sigma-Aldrich Cat#: 798681 tris-HCl Sigma-Aldrich Cat#: 108315 (Continued on next page) iScience 29, 115626, May 15, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NaCl Sigma-Aldrich Cat#: S5886 dynabeads Protein G for Immunoprecipitation Invitrogen Cat#: 10003D LiCl Merck Cat#: 310468 sodium deoxycholate Sigma-Aldrich Cat#: D6750 NaHCO3 Merck Cat#: S6297 proteinase K Thermo Scientific Cat#: EO0491 MNase Thermo Scientific Cat#: EN0181 SYBR Select Master Mix Applied Biosystems Cat#: 4472908 SYBR Safe DNA Gel Stain Invitrogen Cat#: S33102 HEPES Merck Cat#: H3375 MgCl2 Merck Cat#: M8266 CaCl2 Merck Cat#:449709 Critical commercial assays NucleoSpin Gel and PCR Clean-up Kit Macherey-Nagel Cat#: 740609.250 Deposited data Original western blot images Mendeley: https://doi.org/10.17632/ wccscb4vt3.1 N/A Experimental models: Cell lines W8 immortalized mouse embryonic fibroblasts Jenuwein lab W8 D5 immortalized mouse embryonic fibroblasts Jenuwein lab D5 NIH 3T3 HP1α-EGFP immortalized mouse embryonic fibroblasts Jenuwein lab N/A Oligonucleotides MSR_for (5’-TGGAATATGGC GAGAAAACTG) Ching et al.73 N/A MSR_rev (5’-AGGTCCTTCAG TGGGCATTT) Ching et al.73 N/A Recombinant DNA pCAGGS-Suv39h1-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #693 pCAGGS-Suv39h2-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #694 pCAGGS-Suv39h2ΔBD-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #703 pCAGGS-BD-Suv39h1-EGFP-IRES-Puro This study Laboratory of Thomas Jenuwein Database: #696 Software and algorithms R (v4.4.3) R Core Team https://www.r-project.org/ Clustal Omega EMBL-EBI https://www.ebi.ac.uk/Tools/msa/clustalo/ GraphPad Prism 10 GraphPad Software https://www.graphpad.com/ Peptides (v2.4.6) Charif & Lobry, 2007 https://cran.r-project.org/package=Peptides ggplot2 (v3.5.1) Hadley Wickham https://cran.r-project.org/package=ggplot2 ggrepel (v0.9.6) Slowikowski et al. https://cran.r-project.org/package=ggrepel IUPred2 Dosztányi et al., 2005 https://iupred2a.elte.hu/ Zen Black software Zeiss https://www.zeiss.com/microscopy/ int/products/microscope-software/zen.html Fiji (v2.9.0) Schindelin et al., 2012 https://fiji.sc/ Marvin JS (22.11.1) Chemaxon internal http://www.chemaxon.com e2 iScience 29, 115626, May 15, 2026 ..

    other:

    Article Title: Establishment of a broiler jejunal organoid model based on optimized activators or optimized two-step media and its assessment
    Article Snippet: Media compositions were as follows: CMDM: 50% ( v / v ) CM, 37% ( v / v ) Advanced DMEM/F12 (12634010, Gibco, NY, USA), 10% ( v / v ) fetal bovine serum ( FBS ; 16000044, Gibco, NY, USA), 1% ( v/v ) penicillin / streptomycin ( P/S , 15140122, Gibco, NY, USA), 1% ( v / v ) Glutamax (200 mM, 35050061, Gibco, NY, USA), 1% ( v / v ) HEPES (1 M, 15630106, Gibco, NY, USA), 1 mM N-Acetyl- l -Cysteine ( NAC , A7250, Sigma, Tokyo, Japan), 10 μM Y-27632 2HCl (1254, R&D System, MN, USA), 10 μM SB202190 (1614, R&D System, MN, USA), 0.5 μM A83-01 (SML0788, Sigma, Tokyo, Japan), and 50 ng/mL EGF (PMG8043, Gibco, NY, USA).

    Isolation:

    Article Title: T-tubular remodeling occurs in a cardiomyocyte-autonomous manner following pathological mechanical stiffness exposure
    Article Snippet: .. Isolated cardiomyocytes were plated onto 10 μg/ml laminin-coated surfaces and maintained in M199 (ThermoFisher Scientific) supplemented with 1× insulin-transferrinselenium-X (Sigma), 1 μg/μl primocin (InvivoGen), and 20 mM HEPES (ThermoFisher Scientific), pH = 7.4, cultured at 37 °C and 5% CO 2 with the addition of 25 μmol/L of cytochalasin D (Cayman) in the media for 48 h. 10 μg/ml Hoechst 33258 (Invitrogen, H3569), a DNA-binding dye was utilized to stain cardiomyocytes nuclei. ..

    Cell Culture:

    Article Title: T-tubular remodeling occurs in a cardiomyocyte-autonomous manner following pathological mechanical stiffness exposure
    Article Snippet: .. Isolated cardiomyocytes were plated onto 10 μg/ml laminin-coated surfaces and maintained in M199 (ThermoFisher Scientific) supplemented with 1× insulin-transferrinselenium-X (Sigma), 1 μg/μl primocin (InvivoGen), and 20 mM HEPES (ThermoFisher Scientific), pH = 7.4, cultured at 37 °C and 5% CO 2 with the addition of 25 μmol/L of cytochalasin D (Cayman) in the media for 48 h. 10 μg/ml Hoechst 33258 (Invitrogen, H3569), a DNA-binding dye was utilized to stain cardiomyocytes nuclei. ..

    Article Title: The influence of simulated microgravity on MG-63 osteoblast-like cells cultured on polymeric scaffold
    Article Snippet: .. All cell culture media and supplements were purchased from PAN-Biotech, except for non-essential amino acids (NEAA), sodium pyruvate, and (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (HEPES) solution, which was purchased from ThermoFisher. .. Chemical reagents such as Triton X-100, formaldehyde, ALP buffer and NaCl were obtained from Sigma Aldrich, and HCl was obtained from POCh.



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    (a) UNC10088 C -induced conformational change of the loop adjacent to C326. Structural overlay of the SCF FBXO22 -UNC10088 C -NSD2 PWWP1 model (dark gray) and the SCF FBXO22 -BACH1 BTB complex (light gray, PDB: 8UA3) , revealing the displacement of FBXO22 residues Y390. (b) Chemical structures of UNC8732 analogs that contain an aromatic aldehyde in place of the aliphatic amine of UNC8732. (c) Benzaldehyde derivatives UNC10415667 and UNC10415668 promote FBXO22-dependent ubiquitination of *NSD2 PWWP1 when added at 0.5 µM, based on fluorescent scanning of an SDS-PAGE gel. (d) Treatment of U2OS NSD2-HiBit cells with UNC10415667 (1 µM) for 6 hours resulted in a potent reduction of NSD2 levels by about 75%. A more modest effect was observed upon treatment with UNC10415668, and all other compounds had no significant effect. n ≥ 3 independent experiments. Error bars: standard error of the mean. (e) Treatment of U2OS NSD2 HiBit cells with UNC10415667 in a dose response fashion revealed a DC 50 value of 0.46 µM, which is about 2-fold more potent than UNC8732 and UNC10088. n = 3 independent experiments. Error bars: standard error of the mean. (f) UNC10415667 uses C326 to recruit NSD2 to the FBXO22 FIST domain as monitored by size-exclusion chromatography. Chromatograms of different elution profiles between mixtures of WT FBXO22 FIST or a C326A mutant with NSD2 and UNC10415667 (top). Representative Coomassie-stained SDS-PAGE gels of collected fractions. (g) <t>Differential</t> <t>scanning</t> <t>fluorimetry</t> <t>(DSF)</t> was used to monitor the stability of FBXO22 FIST wild type and variants in the presence of UNC10088 or UNC10415667 compared to DMSO, revealing that both compounds stabilize WT FBXO22 FIST and the C326 single cysteine variant (C326 only) as evidenced by an increase in melting temperature (ΔT m ), but not the C326A mutant. n = 3 independent experiments. Error bars: standard error of the mean.
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    (a) UNC10088 C -induced conformational change of the loop adjacent to C326. Structural overlay of the SCF FBXO22 -UNC10088 C -NSD2 PWWP1 model (dark gray) and the SCF FBXO22 -BACH1 BTB complex (light gray, PDB: 8UA3) , revealing the displacement of FBXO22 residues Y390. (b) Chemical structures of UNC8732 analogs that contain an aromatic aldehyde in place of the aliphatic amine of UNC8732. (c) Benzaldehyde derivatives UNC10415667 and UNC10415668 promote FBXO22-dependent ubiquitination of *NSD2 PWWP1 when added at 0.5 µM, based on fluorescent scanning of an SDS-PAGE gel. (d) Treatment of U2OS NSD2-HiBit cells with UNC10415667 (1 µM) for 6 hours resulted in a potent reduction of NSD2 levels by about 75%. A more modest effect was observed upon treatment with UNC10415668, and all other compounds had no significant effect. n ≥ 3 independent experiments. Error bars: standard error of the mean. (e) Treatment of U2OS NSD2 HiBit cells with UNC10415667 in a dose response fashion revealed a DC 50 value of 0.46 µM, which is about 2-fold more potent than UNC8732 and UNC10088. n = 3 independent experiments. Error bars: standard error of the mean. (f) UNC10415667 uses C326 to recruit NSD2 to the FBXO22 FIST domain as monitored by size-exclusion chromatography. Chromatograms of different elution profiles between mixtures of WT FBXO22 FIST or a C326A mutant with NSD2 and UNC10415667 (top). Representative Coomassie-stained SDS-PAGE gels of collected fractions. (g) <t>Differential</t> <t>scanning</t> <t>fluorimetry</t> <t>(DSF)</t> was used to monitor the stability of FBXO22 FIST wild type and variants in the presence of UNC10088 or UNC10415667 compared to DMSO, revealing that both compounds stabilize WT FBXO22 FIST and the C326 single cysteine variant (C326 only) as evidenced by an increase in melting temperature (ΔT m ), but not the C326A mutant. n = 3 independent experiments. Error bars: standard error of the mean.
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    Image Search Results


    (a) UNC10088 C -induced conformational change of the loop adjacent to C326. Structural overlay of the SCF FBXO22 -UNC10088 C -NSD2 PWWP1 model (dark gray) and the SCF FBXO22 -BACH1 BTB complex (light gray, PDB: 8UA3) , revealing the displacement of FBXO22 residues Y390. (b) Chemical structures of UNC8732 analogs that contain an aromatic aldehyde in place of the aliphatic amine of UNC8732. (c) Benzaldehyde derivatives UNC10415667 and UNC10415668 promote FBXO22-dependent ubiquitination of *NSD2 PWWP1 when added at 0.5 µM, based on fluorescent scanning of an SDS-PAGE gel. (d) Treatment of U2OS NSD2-HiBit cells with UNC10415667 (1 µM) for 6 hours resulted in a potent reduction of NSD2 levels by about 75%. A more modest effect was observed upon treatment with UNC10415668, and all other compounds had no significant effect. n ≥ 3 independent experiments. Error bars: standard error of the mean. (e) Treatment of U2OS NSD2 HiBit cells with UNC10415667 in a dose response fashion revealed a DC 50 value of 0.46 µM, which is about 2-fold more potent than UNC8732 and UNC10088. n = 3 independent experiments. Error bars: standard error of the mean. (f) UNC10415667 uses C326 to recruit NSD2 to the FBXO22 FIST domain as monitored by size-exclusion chromatography. Chromatograms of different elution profiles between mixtures of WT FBXO22 FIST or a C326A mutant with NSD2 and UNC10415667 (top). Representative Coomassie-stained SDS-PAGE gels of collected fractions. (g) Differential scanning fluorimetry (DSF) was used to monitor the stability of FBXO22 FIST wild type and variants in the presence of UNC10088 or UNC10415667 compared to DMSO, revealing that both compounds stabilize WT FBXO22 FIST and the C326 single cysteine variant (C326 only) as evidenced by an increase in melting temperature (ΔT m ), but not the C326A mutant. n = 3 independent experiments. Error bars: standard error of the mean.

    Journal: bioRxiv

    Article Title: Structural basis of NSD2 degradation via targeted recruitment of SCF-FBXO22

    doi: 10.1101/2025.08.29.673087

    Figure Lengend Snippet: (a) UNC10088 C -induced conformational change of the loop adjacent to C326. Structural overlay of the SCF FBXO22 -UNC10088 C -NSD2 PWWP1 model (dark gray) and the SCF FBXO22 -BACH1 BTB complex (light gray, PDB: 8UA3) , revealing the displacement of FBXO22 residues Y390. (b) Chemical structures of UNC8732 analogs that contain an aromatic aldehyde in place of the aliphatic amine of UNC8732. (c) Benzaldehyde derivatives UNC10415667 and UNC10415668 promote FBXO22-dependent ubiquitination of *NSD2 PWWP1 when added at 0.5 µM, based on fluorescent scanning of an SDS-PAGE gel. (d) Treatment of U2OS NSD2-HiBit cells with UNC10415667 (1 µM) for 6 hours resulted in a potent reduction of NSD2 levels by about 75%. A more modest effect was observed upon treatment with UNC10415668, and all other compounds had no significant effect. n ≥ 3 independent experiments. Error bars: standard error of the mean. (e) Treatment of U2OS NSD2 HiBit cells with UNC10415667 in a dose response fashion revealed a DC 50 value of 0.46 µM, which is about 2-fold more potent than UNC8732 and UNC10088. n = 3 independent experiments. Error bars: standard error of the mean. (f) UNC10415667 uses C326 to recruit NSD2 to the FBXO22 FIST domain as monitored by size-exclusion chromatography. Chromatograms of different elution profiles between mixtures of WT FBXO22 FIST or a C326A mutant with NSD2 and UNC10415667 (top). Representative Coomassie-stained SDS-PAGE gels of collected fractions. (g) Differential scanning fluorimetry (DSF) was used to monitor the stability of FBXO22 FIST wild type and variants in the presence of UNC10088 or UNC10415667 compared to DMSO, revealing that both compounds stabilize WT FBXO22 FIST and the C326 single cysteine variant (C326 only) as evidenced by an increase in melting temperature (ΔT m ), but not the C326A mutant. n = 3 independent experiments. Error bars: standard error of the mean.

    Article Snippet: 9.5 μL of the master mix containing FBXO22 FIST WT or variants, SYPRO Orange Dye (Invitrogen), and DSF buffer (20 mM Tris 7.5, 150 mM NaCl, and 1 mM TCEP) were added to a 384-well qPCR plate (Genesee Scientific).

    Techniques: Ubiquitin Proteomics, SDS Page, Size-exclusion Chromatography, Mutagenesis, Staining, Variant Assay