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HiMedia Laboratories dropout base growth medium
Dropout Base Growth Medium, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dropout+base+medium/dropout+base+growth+medium/pmc09577237-90-7-11
Average 90 stars, based on 1 article reviews
dropout base growth medium - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Identification and functional characterization of two acyl CoA:diacylglycerol acyltransferase 1 (DGAT1) genes from forage sorghum (Sorghum bicolor) embryo.
Article Snippet: Elevating the lipid content in high-biomass forage crops has emerged as a new research platform for increasing energy density and improving livestock production efficiency associated with improved human health beneficial meat and milk quality.. To gain insights of triacylglycerol (TAG) biosynthesis in forage sorghum, two type-1 diacylglycerol acyltransferase (designated as SbDGAT1-1 and SbDGAT1-2) were characterized for its in vivo function.. SbDGAT1-2 is more abundantly expressed in embryo and bran during the early stage of the grain development in comparison to SbDGAT1-1.

Article Title: Functional characterization of two type-1 diacylglycerol acyltransferase (DGAT1) genes from rice (Oryza sativa) embryo restoring the triacylglycerol accumulation in yeast.
Article Snippet: Key message Two OsDGAT1 genes showed the ability to restore TAG and LB synthesis in yeast H1246.. Alterations in the N-terminal region of OsDGAT1-1 gene revealed its regulatory role in gene function.. Abstract Accumulation of triacylglycerol (TAG) or oil in vegetative tissues has emerged as a promising approach to meet the global needs of food, feed, and fuel.

Article Title: Characterization of oleosin genes from forage sorghum in Arabidopsis and yeast reveals their role in storage lipid stability.
Article Snippet: Main conclusion Overexpression of forage sorghum oleosin genes in Arabidopsis oleosin-deficient mutant and yeast showed increased germination rate, triacylglycerol content, and protection against lipase-mediated TAG degradation.. Abstract Plant lipids are an important source of ration for cattle or other livestock animals to fulfil their energy needs.. Poor energy containing green forages are still one of the major sources of food for livestock animals, leaving the animals undernourished.

Incubation:

Article Title: Loss-of-function mutations in novel triacylglycerol lipase genes are associated with low rancidity in pearl millet flour
Article Snippet: Positive yeast transformants were selected on minimal synthetic defined (SD) base (Takara) media supplemented with complete supplement mixture without uracil (−Ura) (Himedia, India) and further identified by colony PCR using conditions described above. .. Selected single yeast colonies were transferred into dropout base growth medium (Himedia, India) with 2% galactose and 1% raffinose, and incubated at 30 °C for 24 h for lipid and FACS analysis. .. Yeast ΔTGL cells transformed with different PgTAGLip variants were stained with BODIPY 493/503 dye (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-lndacene, Invitrogen) and used to quantify lipid degradation as an indication of lipase gene function.

Article Title: Loss-of-function of triacylglycerol lipases are associated with low flour rancidity in pearl millet [ Pennisetum glaucum (L.) R. Br.]
Article Snippet: Positive yeast transformants were selected on minimal synthetic defined (SD) base (Takara) media supplemented with complete supplement mixture without uracil (-Ura; Himedia, India) and further identified by colony PCR using conditions described above. .. Selected single yeast colonies were transferred into dropout base growth medium (Himedia, India) with 2% galactose and 1% raffinose, and incubated at 30°C for 24 h for lipid and FACS analysis. .. Yeast ΔTGL cells transformed with different PgTAGLip variants were stained with BODIPY 493/503 dye (4,4- Difluoro-1,3,5,7,8- Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene, Invitrogen) and used to quantify lipid degradation as an indication of lipase gene function.

FACS:

Article Title: Loss-of-function mutations in novel triacylglycerol lipase genes are associated with low rancidity in pearl millet flour
Article Snippet: Positive yeast transformants were selected on minimal synthetic defined (SD) base (Takara) media supplemented with complete supplement mixture without uracil (−Ura) (Himedia, India) and further identified by colony PCR using conditions described above. .. Selected single yeast colonies were transferred into dropout base growth medium (Himedia, India) with 2% galactose and 1% raffinose, and incubated at 30 °C for 24 h for lipid and FACS analysis. .. Yeast ΔTGL cells transformed with different PgTAGLip variants were stained with BODIPY 493/503 dye (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-lndacene, Invitrogen) and used to quantify lipid degradation as an indication of lipase gene function.

Article Title: Loss-of-function of triacylglycerol lipases are associated with low flour rancidity in pearl millet [ Pennisetum glaucum (L.) R. Br.]
Article Snippet: Positive yeast transformants were selected on minimal synthetic defined (SD) base (Takara) media supplemented with complete supplement mixture without uracil (-Ura; Himedia, India) and further identified by colony PCR using conditions described above. .. Selected single yeast colonies were transferred into dropout base growth medium (Himedia, India) with 2% galactose and 1% raffinose, and incubated at 30°C for 24 h for lipid and FACS analysis. .. Yeast ΔTGL cells transformed with different PgTAGLip variants were stained with BODIPY 493/503 dye (4,4- Difluoro-1,3,5,7,8- Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene, Invitrogen) and used to quantify lipid degradation as an indication of lipase gene function.



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