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Thermo Fisher blocking solution
Blocking Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dpbs+blocking+solution/PHOSPHATE+BUFFERED+SALINE+DPBS/pmc11003679-68-16-28
Average 99 stars, based on 1 article reviews

blocking solution - by Bioz Stars, 2026-11

99/100 stars

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Article Title: Methyl jasmonate-induced hydroxycinnamoyl putrescine production in Lycium chinense cell suspension cultures
Article Snippet: .. To evaluate the cytoprotective effects against oxidative stress, cells were pretreated with Lc-CK or Lc-MJ for 12 h, washed twice with Dulbecco's phosphate-buffered saline (DPBS; Gibco, USA) to remove residual compounds, and subsequently exposed to 1 mM hydrogen peroxide (H2O2; Sigma-Aldrich, USA) for 1 h. Cell viability following oxidative challenge was determined using the EZ-Cytox assay as described above. ..

Article Title: Toxicological effects of acrylamide on oocyte maturation and subsequent embryonic development in a porcine model.
Article Snippet: A 500 mM stock solution of ACR (A9099; purity ≥ 99%; Sigma-Aldrich) was prepared in distilled water and subsequently diluted with IVM medium to achieve final concentrations of 0, 100, 300, and 500 μM. .. To evaluate nuclear maturation after 44 h of IVM, COCs were transferred to a denuding solution composed of Dulbecco’s phosphatebuffered saline (DPBS; Gibco, Grand Island, NY, USA) supplemented with 100 μg/mL penicillin G, 75 μg/mL streptomycin sulfate, and 0.1% hyaluronidase. .. Following gentle pipetting to remove cumulus cells, denuded oocytes were examined under a microscope (Nikon Corp., Tokyo, Japan) and classified as immature (no polar body extrusion), metaphase II (MII; presence of polar body extrusion), or degenerate.

Article Title: Cytotoxic and Apoptotic Effects of Leptospermum petersonii-Derived 6-Methyltectochrysin in A375 Melanoma Cells
Article Snippet: .. The following solvents were used: hexane and ethyl acetate (Glassworld, Roodepoort, South Africa), methanol (Rochelle Chemicals, Johannesburg, South Africa), dimethyl sulfoxide (DMSO) and etoposide (Merck, Johannesburg, South Africa), silica gel with sieving holes (Merck, Johannesburg, South Africa), A375 melanoma cancer and HEK293 human embryonic kidney normal cells (Japanese Collection of Research Biosources Cell Bank, Ibaraki, Japan), Dulbecco’s Modified Eagle Medium (DMEM) and Dulbecco’s phosphate-buffered saline (DPBS) (Life Technologies Limited, Paisley, UK), Alamar blue reagent (ThermoFisher, Randburg, South Africa), 1 × trypsinEDTA (Life Technologies Corporation, Waltham, MA, USA), Synergy HT microplate reader (Biotek, Santa Clara, CA, USA), the Caspase-GLO 3/7, GoScript Reverse Transcriptase System and GoTaq Green Mastermix (Promega Corporation, Madison, WI, USA), CytoSMART Lux3FL live imaging cell system (Whitehead Scientific, Cape Town, South Africa), FITC Annexin V/Dead Cell Apoptosis for Flow Cytometry Kit Invitrogen (Thermofisher Scientific, Randburg, South Africa), and ReliaPrep RNA Cell Miniprep System (Promega Corporation, Madison, WI, USA). .. Dried Leptospermum petersonii voucher no:85085-43-4 leaves were soaked in 650 mL Hexane for 24 h, then filtered through a Whatman filter disc.

Article Title: Mitochondria-ER-lipid droplet crosstalk regulates metabolic adaptation to shear stress via VMP1.
Article Snippet: Report Mitochondria-ER-lipid droplet crosstalk regulates metabolic adaptation to shear stress via VMP1 Highlights • Shear stress induces transient donut-shaped mitochondria in kidney epithelial cells • Physiological flow transiently stabilizes ER-mitochondria contact sites • Shear stress promotes ER-lipid droplet-mitochondria triple contact-site formation • VMP1 is required for fatty-acid transfer from lipid droplets to mitochondria Authors Maria Laura Rojas, Maribel Lara Corona, Carla Alemany, ..., Ida Chiara Guerrera, Aurore Claude-Taupin, Etienne Morel Correspondence aurore.claude-taupin@inserm.fr (A.C.-T.), etienne.morel@inserm.fr (E.M.) In brief Rojas et al. show that physiological shear stress triggers rapid mitochondrial remodeling and transient stabilization of ER-mitochondria contact sites in kidney epithelial cells.. These contacts recruit lipid droplets, forming triple organelle junctions that facilitate local fatty-acid transfer to mitochondria, a process requiring the ER scramblase VMP1.. Rojas et al, 2026, Current Biology 36, 1–11 October 19, 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies

Article Title: Transforming Growth Factor-β1 Drives Neutrophil Chemokinesis, Not Chemotaxis
Article Snippet: .. Recombinant human TGFβ1 (R&D Systems, 240-B/CF) was reconstituted in 0.1% BSA (Sigma-Aldrich) – Dulbecco’s Phosphate Buffered Saline (DPBS) (Gibco TM ) supplemented with 4 mM hydrochloric acid (Fisher Chemical TM ), denoted as “vehicle” in this study. .. Three μm pore size Nunc TM (ThermoFisher Scientific) polycarbonate cell culture inserts were added to a 24-well plate that contained assay media supplemented with TGFβ1 (0.001–10 ng/ mL) or vehicle in the lower compartment.

Article Title: Development of a multi-targeted sulfonyl-bridged bisselenadiazole derivative as a potent EGFR/PI3K/AKT/mTOR modulator in lung adenocarcinoma.
Article Snippet: .. Attached cells were trypsinized with EDTA and resuspended in 1× Dulbecco's phosphatebuffered saline (DPBS, cat. no. s14190144, Gibco, RSC Adv., 2026, 16, 19903–19919 | 19905 O pe n A cc es s A rt ic le . ..

Article Title: Anticancer effect of Euphorbia tirucalli crude extract in cervical cancer cells: Induction of apoptosis, HDAC inhibition, and gene expression modulation
Article Snippet: .. The cells were washed with 1× Dulbecco's phosphate-buffered saline (DPBS, Thermo Fischer Scientific) and detached using 1× trypsin-EDTA (Thermo Fischer Scientific) for <1 min. Trypsin activity was neutralized by the addition of complete growth medium containing 10% FBS. .. The cell suspension was transferred to a 50 ml Falcon tube (Thermo Fischer Scientific) and centrifuged at 2200 ×g for 4 min.

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Article Title: Food safety of insect and microalgae alternative proteins: IgE cross-reactivity and cytotoxicity evaluation
Article Snippet: Trolox was purchased from Hoffman-La Roche (Basel, Switzerland). .. Recovery Cell Culture Freezing Medium, DMEM, Fetal Bovine Serum (FBS), AntibioticAntimycotic (100×), L-Glutamine 200 mM (100×), and Dulbecco’s phosphate-buffered saline (DPBS) were purchased from Gibco (Gibco, by Thermo Fisher Scientific Inc., NYSE: TMO). .. Flat and round-bottom 96-well microplates were purchased from Corning (Corning, NY, USA).

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