gene exp id1 gg03337774 g1 (Thermo Fisher)
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Structured Review
Thermo Fisher
gene exp id1 gg03337774 g1
Gene Exp Id1 Gg03337774 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/double-loop+control/pmc04571308-234-61--1?v=Thermo+Fisher
Average 85 stars, based on 1 article reviews
Gene Exp Id1 Gg03337774 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/double-loop+control/pmc04571308-234-61--1?v=Thermo+Fisher
Average 85 stars, based on 1 article reviews
gene exp id1 gg03337774 g1 - by Bioz Stars,
2026-08
85/100 stars
Images
1) Product Images from "Synergistic interaction between the fibroblast growth factor and bone morphogenetic protein signaling pathways in lens cells"
Article Title: Synergistic interaction between the fibroblast growth factor and bone morphogenetic protein signaling pathways in lens cells
Journal: Molecular Biology of the Cell
doi: 10.1091/mbc.E15-02-0117
Figure Legend Snippet: Expression of mRNA for the indicated test gene as measured by qRT-PCR (TaqMan), using GAPDH for normalization.
Techniques Used: Expressing
Figure Legend Snippet: FGF specifically increases expression of the BMP signaling reporter BRE-Luc in lens cells. (A–E) DCDMLs were transfected with plasmids encoding BRE-Luc (A–C), SBE4-Luc (D), or 5′ Del 6 Id1-Luc (E) and cultured with no additions (control), 5 ng/ml BMP4, 10 ng/ml FGF2, 1 ng/ml FGF2, 4 ng/ml TGFβ1, or 50 nM TPA for 22 h (A, B, D, E), or 2 h (C). Where indicated, the FGFR inhibitor PD173074 (PD) was also present. Cultures were either fixed and immunostained with anti-luciferase antibodies (A, D, E) or lysed and equal amount of total cell protein analyzed by Western blot using the same antibody (B, C). Representative of three or more independent experiments. (F) Experiments in which a plasmid encoding constitutively expressed EGFP was cotransfected with 5′ Del 6 Id1-Luc at a ratio of 1:4 demonstrated equal levels of expression of EGFP when normalized to either total protein or tubulin, indicating that the lack of expression of 5′ Del 6 Id1-Luc in E was not due to a lack of cell transfection.
Techniques Used: Expressing, Transfection, Cell Culture, Control, Luciferase, Western Blot, Plasmid Preparation