microscope equipped with a double-helix point spread function (dh-psf) phase mask (Double Helix)
90
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Double Helix
microscope equipped with a double-helix point spread function (dh-psf) phase mask
Microscope Equipped With A Double Helix Point Spread Function (Dh Psf) Phase Mask, supplied by Double Helix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/double-helix+point+spread+function+microscope/double+helix+point+spread+function+microscope/pm34328341-109-12-12
Average 90 stars, based on 1 article reviews
Microscope Equipped With A Double Helix Point Spread Function (Dh Psf) Phase Mask, supplied by Double Helix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/double-helix+point+spread+function+microscope/double+helix+point+spread+function+microscope/pm34328341-109-12-12
Average 90 stars, based on 1 article reviews
microscope equipped with a double-helix point spread function (dh-psf) phase mask - by Bioz Stars,
2026-10
90/100 stars
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Microscopy:Article Title: Live-cell three-dimensional single-molecule tracking reveals modulation of enhancer dynamics by NuRD. Article Snippet: .. Analysis of the 20 ms exposure tracks of single CHD4 molecules using our approach revealed a fast unconfined state that was freely Nature Structural & Molecular Biology | Volume 30 | November 2023 | 1628–1639 1630 Article https://doi.org/10.1038/s41594-023-01095-4 b A ng le = Z p os iti on Double-helix point spread function microscopy Sample trajectories Confined Unconfined 0 μm –2 μm +2 μm 10 μm Holo-NuRD complex Separate NuRD subcomplexes Remodeler CHD4 HDAC subcomplex HDAC MBD3 GATA D2A/2B MTA p46/ p48 CHD4 a + MBD3 – MBD3 Step 1 Compute four biophysical parameters using a sliding window for a given trajectory Step 3 GMM classification into two classes: Confined and Unconfined Step 4 Classify subtrajectories by the posterior probability P Sliding window α Fr eq ue nc y Confined Unconfined Unsegmented trajectory Segmented trajectory X(10) 5 100 X(0) Time (s) Po st er io r p ro ba bi lit y P 1.0 0.8 0.6 0.4 0.2 0 Confined Unconfined OutputInput Step 2 Compile biophysical parameters from all sliding windows for all trajectories Trajectory 1 Trajectory 2 Trajectory 3 Trajectory N Dapp (μm 2 s–1) norm||V|| (μm)Lc (nm) α Dapp (μm 2 s–1) norm||V|| (μm)Lc (nm) Dapp (μm 2 s–1) norm||V|| (μm)Lc (nm) 0.7 0.8 0.9 1.0 1.1 2 4 6 8 10 α Time (s) 2 4 6 8 10 Time (s) 2 4 6 8 10 Time (s) 2 4 6 8 10 Time (s) 0.02 0.04 0.06 0.08 0.1 0.2 0.3 0.4 0.5 40 60 80 100 120 140 160 c 0.4 0.8 1.2 120 ||V|| (μU, ∑U) (μC, ∑C) Lc α D 80 40 0 0 0.4 0.8 1.2 120 80 40 160 0.5 1.5 2.5 100 0 300 200 3.5 2 4 6 8 100 500 300 100 700 0 100 300 200 0.5 1.5 2.5 3.5 200 4000 500 300 100 700 2 4 6 8 100 500 300 100 700 500 300 100 0 700 200 400 Fr eq ue nc y Fig. 1 | Live-cell imaging to study NuRD complex binding kinetics and function. a, Schematic representation of the NuRD complex interacting with chromatin in the presence and absence of MBD3. b, Left, single JF549-HaloTagged molecules in the NuRD complex were tracked in 3D using a Article Title: High-resolution dynamic imaging of chromatin DNA communication using Oligo-LiveFISH. Article Snippet: In brief Oligo-LiveFISH is an approach that can track diverse genomic loci at high spatial (20 nm) and temporal (50 ms) resolution and can work in diverse cell types, including primary cells.. Combined with super-localization microscopy and dynamic modeling, Oligo-LiveFISH reveals distinct modes of chromatin communication and the dynamic relationship between transcription and enhancer-promoter interactions. Article Title: Live-cell three-dimensional single-molecule tracking reveals modulation of enhancer dynamics by NuRD Article Snippet: .. We used a Article Title: Effect of loops on the mean-square displacement of Rouse-model chromatin Article Snippet: .. [19] M. P. Backlund, R. Joyner, K. Weis, and W. E. Moerner, Correlations of three-dimensional motion of chromosomal loci in yeast revealed by the Article Title: Autobiography of W. E. (William Esco) Moerner. Article Snippet: .. Mikael P. Backlund, Ryan Joyner, Karsten Weis, and W. E. Moerner, “Correlations of three-dimensional motion of chromosomal loci in yeast revealed by the Live Cell Imaging:Article Title: Live-cell three-dimensional single-molecule tracking reveals modulation of enhancer dynamics by NuRD. Article Snippet: .. Analysis of the 20 ms exposure tracks of single CHD4 molecules using our approach revealed a fast unconfined state that was freely Nature Structural & Molecular Biology | Volume 30 | November 2023 | 1628–1639 1630 Article https://doi.org/10.1038/s41594-023-01095-4 b A ng le = Z p os iti on Double-helix point spread function microscopy Sample trajectories Confined Unconfined 0 μm –2 μm +2 μm 10 μm Holo-NuRD complex Separate NuRD subcomplexes Remodeler CHD4 HDAC subcomplex HDAC MBD3 GATA D2A/2B MTA p46/ p48 CHD4 a + MBD3 – MBD3 Step 1 Compute four biophysical parameters using a sliding window for a given trajectory Step 3 GMM classification into two classes: Confined and Unconfined Step 4 Classify subtrajectories by the posterior probability P Sliding window α Fr eq ue nc y Confined Unconfined Unsegmented trajectory Segmented trajectory X(10) 5 100 X(0) Time (s) Po st er io r p ro ba bi lit y P 1.0 0.8 0.6 0.4 0.2 0 Confined Unconfined OutputInput Step 2 Compile biophysical parameters from all sliding windows for all trajectories Trajectory 1 Trajectory 2 Trajectory 3 Trajectory N Dapp (μm 2 s–1) norm||V|| (μm)Lc (nm) α Dapp (μm 2 s–1) norm||V|| (μm)Lc (nm) Dapp (μm 2 s–1) norm||V|| (μm)Lc (nm) 0.7 0.8 0.9 1.0 1.1 2 4 6 8 10 α Time (s) 2 4 6 8 10 Time (s) 2 4 6 8 10 Time (s) 2 4 6 8 10 Time (s) 0.02 0.04 0.06 0.08 0.1 0.2 0.3 0.4 0.5 40 60 80 100 120 140 160 c 0.4 0.8 1.2 120 ||V|| (μU, ∑U) (μC, ∑C) Lc α D 80 40 0 0 0.4 0.8 1.2 120 80 40 160 0.5 1.5 2.5 100 0 300 200 3.5 2 4 6 8 100 500 300 100 700 0 100 300 200 0.5 1.5 2.5 3.5 200 4000 500 300 100 700 2 4 6 8 100 500 300 100 700 500 300 100 0 700 200 400 Fr eq ue nc y Fig. 1 | Live-cell imaging to study NuRD complex binding kinetics and function. a, Schematic representation of the NuRD complex interacting with chromatin in the presence and absence of MBD3. b, Left, single JF549-HaloTagged molecules in the NuRD complex were tracked in 3D using a Binding Assay:Article Title: Live-cell three-dimensional single-molecule tracking reveals modulation of enhancer dynamics by NuRD. Article Snippet: .. Analysis of the 20 ms exposure tracks of single CHD4 molecules using our approach revealed a fast unconfined state that was freely Nature Structural & Molecular Biology | Volume 30 | November 2023 | 1628–1639 1630 Article https://doi.org/10.1038/s41594-023-01095-4 b A ng le = Z p os iti on Double-helix point spread function microscopy Sample trajectories Confined Unconfined 0 μm –2 μm +2 μm 10 μm Holo-NuRD complex Separate NuRD subcomplexes Remodeler CHD4 HDAC subcomplex HDAC MBD3 GATA D2A/2B MTA p46/ p48 CHD4 a + MBD3 – MBD3 Step 1 Compute four biophysical parameters using a sliding window for a given trajectory Step 3 GMM classification into two classes: Confined and Unconfined Step 4 Classify subtrajectories by the posterior probability P Sliding window α Fr eq ue nc y Confined Unconfined Unsegmented trajectory Segmented trajectory X(10) 5 100 X(0) Time (s) Po st er io r p ro ba bi lit y P 1.0 0.8 0.6 0.4 0.2 0 Confined Unconfined OutputInput Step 2 Compile biophysical parameters from all sliding windows for all trajectories Trajectory 1 Trajectory 2 Trajectory 3 Trajectory N Dapp (μm 2 s–1) norm||V|| (μm)Lc (nm) α Dapp (μm 2 s–1) norm||V|| (μm)Lc (nm) Dapp (μm 2 s–1) norm||V|| (μm)Lc (nm) 0.7 0.8 0.9 1.0 1.1 2 4 6 8 10 α Time (s) 2 4 6 8 10 Time (s) 2 4 6 8 10 Time (s) 2 4 6 8 10 Time (s) 0.02 0.04 0.06 0.08 0.1 0.2 0.3 0.4 0.5 40 60 80 100 120 140 160 c 0.4 0.8 1.2 120 ||V|| (μU, ∑U) (μC, ∑C) Lc α D 80 40 0 0 0.4 0.8 1.2 120 80 40 160 0.5 1.5 2.5 100 0 300 200 3.5 2 4 6 8 100 500 300 100 700 0 100 300 200 0.5 1.5 2.5 3.5 200 4000 500 300 100 700 2 4 6 8 100 500 300 100 700 500 300 100 0 700 200 400 Fr eq ue nc y Fig. 1 | Live-cell imaging to study NuRD complex binding kinetics and function. a, Schematic representation of the NuRD complex interacting with chromatin in the presence and absence of MBD3. b, Left, single JF549-HaloTagged molecules in the NuRD complex were tracked in 3D using a other:Article Title: Silinanyl Rhodamines and Silinanyl Fluoresceins for Super-Resolution Microscopy. Article Snippet: Single-molecule localization microscopy (SMLM) enables the visualization of biomolecules at unprecedented resolution and requires control of the fluorescent blinking (ON/ OFF) states of fluorophores to detect single-molecule fluorescence without overlapping of the signals.. Although SMLM probes based on the intramolecular spirocyclization of Si-xanthene fluorophores have been developed, fluorophores with lower ON/OFF ratios are required for SMLM visualization of high-density structures.. Here, we describe a silinane structure that lowers the ON/OFF ratio of Si-xanthene fluorophores. Article Title: Live-cell three-dimensional single-molecule tracking reveals modulation of enhancer dynamics by NuRD. Article Snippet: We used a |