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Servicebio Inc calcein am pi double staining kit
Calcein Am Pi Double Staining Kit, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/double+staining+kit/am+calcein/pmc13273694-47-7-11
Average 86 stars, based on 1 article reviews
calcein am pi double staining kit - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

other:

Article Title: Versatile Nanotherapeutics for Enhancing Sonodynamic Therapy/Chemotherapy of Thyroid Cancer through Remodeling Tumor Microenvironment and Synergistic Reactive Oxygen Species Augment
Article Snippet: 1,3-Diphenyl isobenzofuran (DPBF) and [Ru(dpp) 3 ]Cl 2 (RDPP) were purchased from Macklin (Shanghai, China).

Staining:

Article Title: Dissolvable silk microneedles delivering minocycline-loaded ZIF-8 nanoparticles for targeted modulation of sebocyte metabolism and improved acne therapy.
Article Snippet: [20] HaCaT and THP-1 cells were seeded in 96-well plates at a density of 5 × 103 cells per well and exposed to 100 μL of each extract for 24 h. Cell viability was evaluated using a Cell Counting Kit-8 (CCK-8; Beyotime, Shanghai, China) according to the manufacturer’s instructions. .. AR TIC LE IN PR ES S ARTICLE IN PRESS After extract exposure, the cells were washed with PBS and stained with CalceinAM/PI working solution (2 μL each per 1 mL buffer; Servicebio, Wuhan, China) at 37 °C for 20 min in the dark. .. Fluorescence images were acquired using a Nikon Eclipse Ti-U microscope (Nikon, Tokyo, Japan), where green and red fluorescence indicated viable and dead cells, respectively.

Article Title: Bisphenol A potentiates ischemia-reperfusion-induced endothelial and blood-brain barrier dysfunction associated with CX3CL1-CX3CR1 signaling.
Article Snippet: Cell viability was assessed using a CCK-8 assay (BS350B, Biosharp, China). .. Live/dead cell staining was performed using a Calcein-AM/PI kit (G1707, Servicebio, China) to evaluate cytotoxicity after OGD/R and BPA treatment. .. Cells were incubated with Calcein-AM and PI working solution at 37 ◦C in the dark and immediately imaged using a fluorescence microscope (BX53, Olympus, Japan).

Article Title: Engineering multifunctional chitosan-based hydrogels for integrated antimicrobial therapy and endogenous stem cell-driven tissue repair in infectious keratitis
Article Snippet: .. Cells were exposed to QUDCS/OD hydrogels at varying concentrations for 24 h. Cell viability was assessed using calcein-AM/PI dual staining (G1707, Servicebio, China) according to the manufacturer's protocol. .. Cells were exposed to QUDCS/OD hydrogels at varying concentrations for 24 h. Cell viability was assessed using calcein-AM/PI dual staining (G1707, Servicebio, China) according to the manufacturer's protocol.

Incubation:

Article Title: Aloe vera polysaccharides facilitate diabetic wound healing by promoting macrophage M 2 polarization through inhibition of NLRP3 inflammasome activation.
Article Snippet: Background: Diabetic wound healing impairment poses a serious threat to patient health, underscoring the urgent need for effective therapies.. Aloe polysaccharides (APs), one of the main bioactive components of Aloe vera, have been reported to exhibit diverse biological activities, including antibacterial, antioxidant, anti-obesity, and antitumor effects.. However, their role in diabetic wound repair remains unclear, limiting its translational potential.

Fluorescence:

Article Title: Aloe vera polysaccharides facilitate diabetic wound healing by promoting macrophage M 2 polarization through inhibition of NLRP3 inflammasome activation.
Article Snippet: Background: Diabetic wound healing impairment poses a serious threat to patient health, underscoring the urgent need for effective therapies.. Aloe polysaccharides (APs), one of the main bioactive components of Aloe vera, have been reported to exhibit diverse biological activities, including antibacterial, antioxidant, anti-obesity, and antitumor effects.. However, their role in diabetic wound repair remains unclear, limiting its translational potential.

Microscopy:

Article Title: Aloe vera polysaccharides facilitate diabetic wound healing by promoting macrophage M 2 polarization through inhibition of NLRP3 inflammasome activation.
Article Snippet: Background: Diabetic wound healing impairment poses a serious threat to patient health, underscoring the urgent need for effective therapies.. Aloe polysaccharides (APs), one of the main bioactive components of Aloe vera, have been reported to exhibit diverse biological activities, including antibacterial, antioxidant, anti-obesity, and antitumor effects.. However, their role in diabetic wound repair remains unclear, limiting its translational potential.

Double Staining:

Article Title: Lapatinib induces ferroptosis in osteosarcoma via the SLC1A5-GPX4 axis
Article Snippet: .. Cell viability was qualitatively assessed using a Calcein-AM/PI double-staining kit (G1707, Servicebio, Wuhan, China). ..



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Image Search Results


Biocompatibility of patches: (a–c) sensitisation test of transdermal patch in SD rats skin, (d) cell viability using patch leachate by CCK-8 kit and (e) images of live-dead staining after 24 h (scale bar 100 μm).

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: A melatonin textile transdermal patch based on physicochemical synergistic permeation-enhancement strategy for circadian rhythm regulation

doi: 10.3389/fbioe.2026.1913854

Figure Lengend Snippet: Biocompatibility of patches: (a–c) sensitisation test of transdermal patch in SD rats skin, (d) cell viability using patch leachate by CCK-8 kit and (e) images of live-dead staining after 24 h (scale bar 100 μm).

Article Snippet: HaCaT cells were cultured with the patch extract, and cell viability in the extract was measured at 450 nm using a microplate reader after 24 h. Following 24-h culture in the patch extract, HaCaT cells were stained with the Live/Dead Double Staining Kit (Beyotime, Shanghai, China) and observed under a fluorescence microscope (Ex/Em = 490/515 nm for Calcein-AM and Ex/Em = 535/617 nm for PI).

Techniques: CCK-8 Assay, Staining