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cds sequence of dnmt3a  (Shanghai GenePharma)

 
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    Shanghai GenePharma cds sequence of dnmt3a
    Cds Sequence Of Dnmt3a, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dnmt3a+sequence/dnmt1+sirna/pm40456336-48-4-8
    Average 90 stars, based on 1 article reviews
    cds sequence of dnmt3a - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: DNMT3B promotes the progression of pheochromocytoma by mediating the hypermethylation of LRP1B promoter.
    Article Snippet: The LRP1B overexpression vector (oe-LRP1B-1, oe-LRP1B-2, and oe-LRP1B-3), shRNA containing DNMT3B target (sh-DNMT3B-1, sh-DNMT3B-2, and sh-DNMT3B-3), sh-DNMT1, sh-DNMT3A, and its negative control (NC) were all designed and synthesized by GenePharma (Shanghai, China).

    Article Title: circKCNQ5 promotes the proliferation of DNA-methyltransferase 3A R882 mutated acute myeloid leukemia cells by elevating high-mobility group box 1 expression.
    Article Snippet: Wild-type DNmt3a (DNmt3a-Wt) or DR882mUt (R882H, base 2645 g > a) were synthetized by genePharma (Shanghai, China) and inserted into lentiviral vector plvx-pgk-zsgreen-puro. then the lentiviral plasmid and packaging plasmid were transfected into 293 t cells together. after culture 3 days, the lentiviruses in cell culture supernatant were collected and concentrate it for storage. then stably expressing cell lines were selected using puromycin.

    Sequencing:

    Article Title: Regulatory mechanism of DNMT3A in interferon expression inhibition by Newcastle disease virus V protein.
    Article Snippet: Newcastle disease (ND), caused by the Newcastle disease virus (NDV) and characterised by rapid onset and high mortality rates, is a highly contagious disease in the poultry industry.. Interferons (IFN) play a key role in host defence against NDV, however, the non-structural protein V of NDV can antagonise IFN to facilitate NDV immune escape.. DNA Methyltransferase (DNMT)3A, an important IFN signaling molecules regulator, may participate in the process by which the V protein inhibits IFN.

    Synthesized:

    Article Title: Exosome-derived miR-548ag drives hepatic lipid accumulation via upregulating FASN through inhibition of DNMT3B.
    Article Snippet: .. The miR-548ag mimic, miR-548ag inhibitor, and DNMT3B siRNA used in this study were synthesized by Shanghai GenePharma Co., Ltd., and the DNMT3B overexpression plasmid was obtained from Beijing HeSheng Biotechnology Co., Ltd. ..

    Over Expression:

    Article Title: Exosome-derived miR-548ag drives hepatic lipid accumulation via upregulating FASN through inhibition of DNMT3B.
    Article Snippet: .. The miR-548ag mimic, miR-548ag inhibitor, and DNMT3B siRNA used in this study were synthesized by Shanghai GenePharma Co., Ltd., and the DNMT3B overexpression plasmid was obtained from Beijing HeSheng Biotechnology Co., Ltd. ..

    Plasmid Preparation:

    Article Title: Exosome-derived miR-548ag drives hepatic lipid accumulation via upregulating FASN through inhibition of DNMT3B.
    Article Snippet: .. The miR-548ag mimic, miR-548ag inhibitor, and DNMT3B siRNA used in this study were synthesized by Shanghai GenePharma Co., Ltd., and the DNMT3B overexpression plasmid was obtained from Beijing HeSheng Biotechnology Co., Ltd. ..

    Control:

    Article Title: Environmental interpretation of the impact of vehicle emissions on prostate cancer progression
    Article Snippet: The primer sequences for DNMT3B (forward primer: AGGGAAGACTCGATCCTCGTC, reverse primer: GTGTGTAGCTTAGCAGACTGG) and ACTIN (forward primer: TGGCACCCAGCACAATGAA, reverse primer: CTAAGTCATAGTCCGCCTAGAAGCA) were purchased from GenePharma (Shanghai, China). .. DNMT3B siRNA oligonucleotides (si-1: 5’-CCACCUUCAAUAAGCUCGUTT-3’, si-2: 5’-CGCCUCAAGACAAAUUGCUTT-3’) and control siRNA (si-NC: 5’-UUCUCCGAACGUGUCACGUTT-3’) were obtained from GenePharma (Shanghai, China). ..



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    Image Search Results


    Schematic overview of the CRISPR/dCas9- dnmt3a editing system for the emx2 promoter region of Chinese tongue sole testis cells. ( A ) Edit system schematic diagram. ( B ) dCas9- dnmt3a plasmid structure diagram and sgRNA plasmid structure diagram. ( C ) The sequence of the sgRNAs. ( D ) Bisulfite sequencing positions and sgRNA sites of emx2 ; lollipop chart on the left represents bisulfite sequencing of the CpGs’ position. The arrows indicate the target sequence of the designed sgRNAs, with the direction of the arrows aligning with the target sequence.

    Journal: International Journal of Molecular Sciences

    Article Title: CRISPR/dCas9-Mediated DNA Methylation Editing on emx2 in Chinese Tongue Sole ( Cynoglossus semilaevis ) Testis Cells

    doi: 10.3390/ijms25147637

    Figure Lengend Snippet: Schematic overview of the CRISPR/dCas9- dnmt3a editing system for the emx2 promoter region of Chinese tongue sole testis cells. ( A ) Edit system schematic diagram. ( B ) dCas9- dnmt3a plasmid structure diagram and sgRNA plasmid structure diagram. ( C ) The sequence of the sgRNAs. ( D ) Bisulfite sequencing positions and sgRNA sites of emx2 ; lollipop chart on the left represents bisulfite sequencing of the CpGs’ position. The arrows indicate the target sequence of the designed sgRNAs, with the direction of the arrows aligning with the target sequence.

    Article Snippet: The dnmt3a sequence and the fuw-dCas9 plasmid (Addgene #84476, Watertown, MA, USA) were digested with HamI (NEB) and EcoRI (NEB) enzymes at 37 °C for 2 h, respectively.

    Techniques: CRISPR, Plasmid Preparation, Sequencing, Methylation Sequencing

    CRISPR/dCas9-mediated DNA methylation editing on emx2 gene. DNA methylation status of the emx2 promoter region with the ( A ) blank control; ( B ) dCas9- dnmt3a ; ( C ) dCas9- dnmt3a and sgRNA1; ( D ) dCas9- dnmt3a and sgRNA2; ( E ) dCas9- dnmt3a and sgRNA3; ( F ) dCas9- dnmt3a and sgRNA4; ( G ) dCas9- dnmt3a and sgRNA5; ( H ) dCas9- dnmt3a and sgRNA6; ( I ) dCas9- dnmt3a and sgRNA1+3; ( J ) Percentage of DNA methylation levels at emx2 promoter region with different treatments; ( K ) Relative expression of emx2 after transfection of dCas9- dnmt3a and different sgRNAs. Filled (black) circles correspond to methylated CpGs, unfilled (white) circles correspond to unmethylated CpGs. Upper black lines indicate statistical difference among blank control and sgRNA groups, while lines below the graph highlight that the sgRNAs were co-transfected with the CRISPR/dCas9- dnmt3a vector. The asterisks (*) are used to denote significant differences among groups using two-way ANOVA and Tukey’s multiple comparisons. *** p < 0.001, **** p < 0.0001.

    Journal: International Journal of Molecular Sciences

    Article Title: CRISPR/dCas9-Mediated DNA Methylation Editing on emx2 in Chinese Tongue Sole ( Cynoglossus semilaevis ) Testis Cells

    doi: 10.3390/ijms25147637

    Figure Lengend Snippet: CRISPR/dCas9-mediated DNA methylation editing on emx2 gene. DNA methylation status of the emx2 promoter region with the ( A ) blank control; ( B ) dCas9- dnmt3a ; ( C ) dCas9- dnmt3a and sgRNA1; ( D ) dCas9- dnmt3a and sgRNA2; ( E ) dCas9- dnmt3a and sgRNA3; ( F ) dCas9- dnmt3a and sgRNA4; ( G ) dCas9- dnmt3a and sgRNA5; ( H ) dCas9- dnmt3a and sgRNA6; ( I ) dCas9- dnmt3a and sgRNA1+3; ( J ) Percentage of DNA methylation levels at emx2 promoter region with different treatments; ( K ) Relative expression of emx2 after transfection of dCas9- dnmt3a and different sgRNAs. Filled (black) circles correspond to methylated CpGs, unfilled (white) circles correspond to unmethylated CpGs. Upper black lines indicate statistical difference among blank control and sgRNA groups, while lines below the graph highlight that the sgRNAs were co-transfected with the CRISPR/dCas9- dnmt3a vector. The asterisks (*) are used to denote significant differences among groups using two-way ANOVA and Tukey’s multiple comparisons. *** p < 0.001, **** p < 0.0001.

    Article Snippet: The dnmt3a sequence and the fuw-dCas9 plasmid (Addgene #84476, Watertown, MA, USA) were digested with HamI (NEB) and EcoRI (NEB) enzymes at 37 °C for 2 h, respectively.

    Techniques: CRISPR, DNA Methylation Assay, Control, Expressing, Transfection, Methylation, Plasmid Preparation

    Off-target effects of CRISPR/dCas9- dnmt3a system. DNA methylation of the promoter region of ( A ) slc20a2 ; ( B ) ddx17 ; ( C ) katb6 and ( D ) xpa and relative expression of potential off-target genes ( E ) slc20a2 ; ( F ) ddx17 ; ( G ) katb6 and ( H ) xpa . Upper black lines indicate statistical difference between the blank control and sgRNA3 groups, while lines below the graph highlight that the sgRNAs were co-transfected with the CRISPR/dCas9- dnmt3a vector. The asterisks (*) are used to denote significant differences between groups using two-way ANOVA and Tukey’s multiple comparisons. *** p < 0.001.

    Journal: International Journal of Molecular Sciences

    Article Title: CRISPR/dCas9-Mediated DNA Methylation Editing on emx2 in Chinese Tongue Sole ( Cynoglossus semilaevis ) Testis Cells

    doi: 10.3390/ijms25147637

    Figure Lengend Snippet: Off-target effects of CRISPR/dCas9- dnmt3a system. DNA methylation of the promoter region of ( A ) slc20a2 ; ( B ) ddx17 ; ( C ) katb6 and ( D ) xpa and relative expression of potential off-target genes ( E ) slc20a2 ; ( F ) ddx17 ; ( G ) katb6 and ( H ) xpa . Upper black lines indicate statistical difference between the blank control and sgRNA3 groups, while lines below the graph highlight that the sgRNAs were co-transfected with the CRISPR/dCas9- dnmt3a vector. The asterisks (*) are used to denote significant differences between groups using two-way ANOVA and Tukey’s multiple comparisons. *** p < 0.001.

    Article Snippet: The dnmt3a sequence and the fuw-dCas9 plasmid (Addgene #84476, Watertown, MA, USA) were digested with HamI (NEB) and EcoRI (NEB) enzymes at 37 °C for 2 h, respectively.

    Techniques: CRISPR, DNA Methylation Assay, Expressing, Control, Transfection, Plasmid Preparation

    Effects on downstream genes after the emx2 promoter region methylated. Relative expression of downstream genes ( A ) myc , ( B ) klf4 , and ( C ) wnt1 . Upper black lines indicate statistical difference among blank control and sgRNA groups for myc expression, while lines below the graph highlight that the sgRNAs were co-transfected with the CRISPR/dCas9- dnmt3a vector. The asterisks (*) are used to denote significant differences between groups using two-way ANOVA and Tukey’s multiple comparisons. *** p < 0.001.

    Journal: International Journal of Molecular Sciences

    Article Title: CRISPR/dCas9-Mediated DNA Methylation Editing on emx2 in Chinese Tongue Sole ( Cynoglossus semilaevis ) Testis Cells

    doi: 10.3390/ijms25147637

    Figure Lengend Snippet: Effects on downstream genes after the emx2 promoter region methylated. Relative expression of downstream genes ( A ) myc , ( B ) klf4 , and ( C ) wnt1 . Upper black lines indicate statistical difference among blank control and sgRNA groups for myc expression, while lines below the graph highlight that the sgRNAs were co-transfected with the CRISPR/dCas9- dnmt3a vector. The asterisks (*) are used to denote significant differences between groups using two-way ANOVA and Tukey’s multiple comparisons. *** p < 0.001.

    Article Snippet: The dnmt3a sequence and the fuw-dCas9 plasmid (Addgene #84476, Watertown, MA, USA) were digested with HamI (NEB) and EcoRI (NEB) enzymes at 37 °C for 2 h, respectively.

    Techniques: Methylation, Expressing, Control, Transfection, CRISPR, Plasmid Preparation