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pcdna3.1 vectors sub-cloned with the whole sequence of dnmt3a  (Ribobio co)

 
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    Ribobio co pcdna3.1 vectors sub-cloned with the whole sequence of dnmt3a
    Pcdna3.1 Vectors Sub Cloned With The Whole Sequence Of Dnmt3a, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dnmt3a+sequence/dnmt1+sirna/pm36376550-32-9-17
    Average 90 stars, based on 1 article reviews
    pcdna3.1 vectors sub-cloned with the whole sequence of dnmt3a - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Over Expression:

    Article Title: DNMT1-Mediated the Downregulation of FOXF1 Promotes High Glucose-induced Podocyte Damage by Regulating the miR-342-3p/E2F1 Axis.
    Article Snippet: Podocyte damage plays a crucial role in the occurrence and development of diabetic nephropathy (DN).. Accumulating evidence suggests that dysregulation of transcription factors plays a crucial role in podocyte damage in DN.. However, the biological functions and underlying mechanisms of most transcription factors in hyperglycemia-induced podocytes damage remain largely unknown.

    Negative Control:

    Article Title: DNMT1-Mediated the Downregulation of FOXF1 Promotes High Glucose-induced Podocyte Damage by Regulating the miR-342-3p/E2F1 Axis.
    Article Snippet: Podocyte damage plays a crucial role in the occurrence and development of diabetic nephropathy (DN).. Accumulating evidence suggests that dysregulation of transcription factors plays a crucial role in podocyte damage in DN.. However, the biological functions and underlying mechanisms of most transcription factors in hyperglycemia-induced podocytes damage remain largely unknown.

    Article Title: ORC1 enhances repressive epigenetic modifications on HIV-1 LTR to promote HIV-1 latency.
    Article Snippet: The human immunodeficiency virus type 1 (HIV-1) reservoir consists of latently infected cells which present a major obstacle to achieving a functional cure for HIV-1.. The formation and maintenance of HIV-1 latency have been extensively studied, and latency-reversing agents (LRAs) that can reactivate latent HIV-1 by targeting the involved host factors are developed; however, their clinical efficacies remain unsatisfac tory.. Therefore, it is imperative to identify novel targets for more potential candidates or better combinations for LRAs.

    other:

    Article Title: BCL7A inhibits the progression and drug-resistance in acute myeloid leukemia.
    Article Snippet: Short hairpin RNAs used for the knockdown of DNMT1, DNMT3A, DNMT3B, PTBP1 and IRF7 (sh-DNMT1, shDNMT3A, sh-DNMT3B) and control sh-NC were provided by the Ribobio Inc. (Guangzhou, Guangdong, China).

    Article Title: DNMT3A facilitates breast cancer progression via regulating ADAMTS8 mediated EGFR-MEK-ERK activation.
    Article Snippet: DNMT3A resistant small interfering RNAs (si-DNMT3A-1, si-DNMT3A-2, and si-DNMT3A-3, si-ADAMTS8–1, si-ADAMTS8–2, si-ADAMTS8–3, si-NC) were synthesized by RiboBio Biotechnology Co., LTD (Guangzhou, China).

    Article Title: ETS2 overexpression ameliorates cartilage injury in osteoarthritis by the ETS2/miR-155/STAT1/DNMT1 feedback loop pathway.
    Article Snippet: Osteoarthritis (OA) is the most common irreversible chronic joint dysfunction disease, which is pathologically characterized by disturbance of articular cartilage homeostasis leading to subsequent inflammatory response and cartilage extracellular matrix (ECM) degradation.. Increasing evidence has demonstrated the dysregulation of transcription factors play crucial roles in the occurrence and development of osteoarthritis (OA), but the potential functions and mechanism of most transcription factors in OA has not been completely illuminated.. In this study, we identified that transcription factor V-ets erythroblastosis virus E26 oncogene homolog 2 (ETS2) was significantly down-regulated in OA cartilage and IL-1β-induced OA chondrocytes.

    Article Title: Epigenetic PPARγ preservation attenuates temporomandibular joint osteoarthritis.
    Article Snippet: Objective: Previous studies have demonstrated that PPARγ deficiency is associated with osteoarthritis in the knee joint.. However, whether epigenetic PPARγ dysregulation has any effect on temporomandibular joint osteoarthritis (TMJOA) is unknown.. This study aims to determine the role and mechanism of epigenetic PPARγ dysregulation in TMJOA.

    Mutagenesis:

    Article Title: Co-transcriptional R-loops-mediated epigenetic regulation drives growth retardation and docetaxel chemosensitivity enhancement in advanced prostate cancer
    Article Snippet: .. Antibodies used in this study were: anti-Myc tag (ab32, Abcam), anti-IGF2BP1 (ab290736, Abcam), anti-IGF2BP2 (ab128175, Abcam), anti-IGF2BP3 (ab177477, Abcam), anti-RBM15 (10587-1-AP, Proteintech), anti-DNMT1 (ab92314, Abcam), anti-DNMT3A (ab307503, Abcam), anti-DNMT3B(67,259, Cell Signaling Technology), anti-SEMA3F (SAB2107196, Sigma), anti-S9.6 (MABE1095, Sigma), anti-5-mC (ab214727, Abcam), anti-m 6 A (SAB5600251, Sigma), anti-YAP1(phosphor S127, ab76252, Abcam), anti-YAP1(66900-1-Ig, Proteintech), anti-LATS1(66569-1-Ig, Proteintech), anti-LATS2(20276-1-AP, Proteintech), anti-β-Actin(4970, Cell Signaling Technology). siRNAs against DNMT1 used were purchased from Ribobio Co.,Ltd (Guangzhou, China). pcDNA3-based vectors encoding wild-type, RRM domain mutant, KH domain mutant Myc-tagged IGF2BP1, IGF2BP2, IGF2BP3 were produced by Shanghai Yoche Biotechnology Co.,Ltd (Shanghai, China). .. The plasmids encoding RBM15 and SEMA3F (h-RBM15-pcDNA3.1-c-HA, M35-FLAG-SEMA3F) were obtained from Guangzhou FulenGen Co., Ltd (Guangzhou, China).

    Produced:

    Article Title: Co-transcriptional R-loops-mediated epigenetic regulation drives growth retardation and docetaxel chemosensitivity enhancement in advanced prostate cancer
    Article Snippet: .. Antibodies used in this study were: anti-Myc tag (ab32, Abcam), anti-IGF2BP1 (ab290736, Abcam), anti-IGF2BP2 (ab128175, Abcam), anti-IGF2BP3 (ab177477, Abcam), anti-RBM15 (10587-1-AP, Proteintech), anti-DNMT1 (ab92314, Abcam), anti-DNMT3A (ab307503, Abcam), anti-DNMT3B(67,259, Cell Signaling Technology), anti-SEMA3F (SAB2107196, Sigma), anti-S9.6 (MABE1095, Sigma), anti-5-mC (ab214727, Abcam), anti-m 6 A (SAB5600251, Sigma), anti-YAP1(phosphor S127, ab76252, Abcam), anti-YAP1(66900-1-Ig, Proteintech), anti-LATS1(66569-1-Ig, Proteintech), anti-LATS2(20276-1-AP, Proteintech), anti-β-Actin(4970, Cell Signaling Technology). siRNAs against DNMT1 used were purchased from Ribobio Co.,Ltd (Guangzhou, China). pcDNA3-based vectors encoding wild-type, RRM domain mutant, KH domain mutant Myc-tagged IGF2BP1, IGF2BP2, IGF2BP3 were produced by Shanghai Yoche Biotechnology Co.,Ltd (Shanghai, China). .. The plasmids encoding RBM15 and SEMA3F (h-RBM15-pcDNA3.1-c-HA, M35-FLAG-SEMA3F) were obtained from Guangzhou FulenGen Co., Ltd (Guangzhou, China).

    Knockdown:

    Article Title: ORC1 enhances repressive epigenetic modifications on HIV-1 LTR to promote HIV-1 latency.
    Article Snippet: The human immunodeficiency virus type 1 (HIV-1) reservoir consists of latently infected cells which present a major obstacle to achieving a functional cure for HIV-1.. The formation and maintenance of HIV-1 latency have been extensively studied, and latency-reversing agents (LRAs) that can reactivate latent HIV-1 by targeting the involved host factors are developed; however, their clinical efficacies remain unsatisfac tory.. Therefore, it is imperative to identify novel targets for more potential candidates or better combinations for LRAs.

    Real-time Polymerase Chain Reaction:

    Article Title: ORC1 enhances repressive epigenetic modifications on HIV-1 LTR to promote HIV-1 latency.
    Article Snippet: The human immunodeficiency virus type 1 (HIV-1) reservoir consists of latently infected cells which present a major obstacle to achieving a functional cure for HIV-1.. The formation and maintenance of HIV-1 latency have been extensively studied, and latency-reversing agents (LRAs) that can reactivate latent HIV-1 by targeting the involved host factors are developed; however, their clinical efficacies remain unsatisfac tory.. Therefore, it is imperative to identify novel targets for more potential candidates or better combinations for LRAs.

    Western Blot:

    Article Title: ORC1 enhances repressive epigenetic modifications on HIV-1 LTR to promote HIV-1 latency.
    Article Snippet: The human immunodeficiency virus type 1 (HIV-1) reservoir consists of latently infected cells which present a major obstacle to achieving a functional cure for HIV-1.. The formation and maintenance of HIV-1 latency have been extensively studied, and latency-reversing agents (LRAs) that can reactivate latent HIV-1 by targeting the involved host factors are developed; however, their clinical efficacies remain unsatisfac tory.. Therefore, it is imperative to identify novel targets for more potential candidates or better combinations for LRAs.

    Synthesized:

    Article Title: Integration of RRBS and RNA-seq unravels the regulatory role of DNMT3A in porcine Sertoli cell proliferation
    Article Snippet: .. Three RNAi oligonucleotides (si- DNMT3A -1, -2, -3) were synthesized and provided by RiboBio (Guangzhou, China), with their respective sequences detailed in . ..



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    Image Search Results


    Schematic overview of the CRISPR/dCas9- dnmt3a editing system for the emx2 promoter region of Chinese tongue sole testis cells. ( A ) Edit system schematic diagram. ( B ) dCas9- dnmt3a plasmid structure diagram and sgRNA plasmid structure diagram. ( C ) The sequence of the sgRNAs. ( D ) Bisulfite sequencing positions and sgRNA sites of emx2 ; lollipop chart on the left represents bisulfite sequencing of the CpGs’ position. The arrows indicate the target sequence of the designed sgRNAs, with the direction of the arrows aligning with the target sequence.

    Journal: International Journal of Molecular Sciences

    Article Title: CRISPR/dCas9-Mediated DNA Methylation Editing on emx2 in Chinese Tongue Sole ( Cynoglossus semilaevis ) Testis Cells

    doi: 10.3390/ijms25147637

    Figure Lengend Snippet: Schematic overview of the CRISPR/dCas9- dnmt3a editing system for the emx2 promoter region of Chinese tongue sole testis cells. ( A ) Edit system schematic diagram. ( B ) dCas9- dnmt3a plasmid structure diagram and sgRNA plasmid structure diagram. ( C ) The sequence of the sgRNAs. ( D ) Bisulfite sequencing positions and sgRNA sites of emx2 ; lollipop chart on the left represents bisulfite sequencing of the CpGs’ position. The arrows indicate the target sequence of the designed sgRNAs, with the direction of the arrows aligning with the target sequence.

    Article Snippet: The dnmt3a sequence and the fuw-dCas9 plasmid (Addgene #84476, Watertown, MA, USA) were digested with HamI (NEB) and EcoRI (NEB) enzymes at 37 °C for 2 h, respectively.

    Techniques: CRISPR, Plasmid Preparation, Sequencing, Methylation Sequencing

    CRISPR/dCas9-mediated DNA methylation editing on emx2 gene. DNA methylation status of the emx2 promoter region with the ( A ) blank control; ( B ) dCas9- dnmt3a ; ( C ) dCas9- dnmt3a and sgRNA1; ( D ) dCas9- dnmt3a and sgRNA2; ( E ) dCas9- dnmt3a and sgRNA3; ( F ) dCas9- dnmt3a and sgRNA4; ( G ) dCas9- dnmt3a and sgRNA5; ( H ) dCas9- dnmt3a and sgRNA6; ( I ) dCas9- dnmt3a and sgRNA1+3; ( J ) Percentage of DNA methylation levels at emx2 promoter region with different treatments; ( K ) Relative expression of emx2 after transfection of dCas9- dnmt3a and different sgRNAs. Filled (black) circles correspond to methylated CpGs, unfilled (white) circles correspond to unmethylated CpGs. Upper black lines indicate statistical difference among blank control and sgRNA groups, while lines below the graph highlight that the sgRNAs were co-transfected with the CRISPR/dCas9- dnmt3a vector. The asterisks (*) are used to denote significant differences among groups using two-way ANOVA and Tukey’s multiple comparisons. *** p < 0.001, **** p < 0.0001.

    Journal: International Journal of Molecular Sciences

    Article Title: CRISPR/dCas9-Mediated DNA Methylation Editing on emx2 in Chinese Tongue Sole ( Cynoglossus semilaevis ) Testis Cells

    doi: 10.3390/ijms25147637

    Figure Lengend Snippet: CRISPR/dCas9-mediated DNA methylation editing on emx2 gene. DNA methylation status of the emx2 promoter region with the ( A ) blank control; ( B ) dCas9- dnmt3a ; ( C ) dCas9- dnmt3a and sgRNA1; ( D ) dCas9- dnmt3a and sgRNA2; ( E ) dCas9- dnmt3a and sgRNA3; ( F ) dCas9- dnmt3a and sgRNA4; ( G ) dCas9- dnmt3a and sgRNA5; ( H ) dCas9- dnmt3a and sgRNA6; ( I ) dCas9- dnmt3a and sgRNA1+3; ( J ) Percentage of DNA methylation levels at emx2 promoter region with different treatments; ( K ) Relative expression of emx2 after transfection of dCas9- dnmt3a and different sgRNAs. Filled (black) circles correspond to methylated CpGs, unfilled (white) circles correspond to unmethylated CpGs. Upper black lines indicate statistical difference among blank control and sgRNA groups, while lines below the graph highlight that the sgRNAs were co-transfected with the CRISPR/dCas9- dnmt3a vector. The asterisks (*) are used to denote significant differences among groups using two-way ANOVA and Tukey’s multiple comparisons. *** p < 0.001, **** p < 0.0001.

    Article Snippet: The dnmt3a sequence and the fuw-dCas9 plasmid (Addgene #84476, Watertown, MA, USA) were digested with HamI (NEB) and EcoRI (NEB) enzymes at 37 °C for 2 h, respectively.

    Techniques: CRISPR, DNA Methylation Assay, Control, Expressing, Transfection, Methylation, Plasmid Preparation

    Off-target effects of CRISPR/dCas9- dnmt3a system. DNA methylation of the promoter region of ( A ) slc20a2 ; ( B ) ddx17 ; ( C ) katb6 and ( D ) xpa and relative expression of potential off-target genes ( E ) slc20a2 ; ( F ) ddx17 ; ( G ) katb6 and ( H ) xpa . Upper black lines indicate statistical difference between the blank control and sgRNA3 groups, while lines below the graph highlight that the sgRNAs were co-transfected with the CRISPR/dCas9- dnmt3a vector. The asterisks (*) are used to denote significant differences between groups using two-way ANOVA and Tukey’s multiple comparisons. *** p < 0.001.

    Journal: International Journal of Molecular Sciences

    Article Title: CRISPR/dCas9-Mediated DNA Methylation Editing on emx2 in Chinese Tongue Sole ( Cynoglossus semilaevis ) Testis Cells

    doi: 10.3390/ijms25147637

    Figure Lengend Snippet: Off-target effects of CRISPR/dCas9- dnmt3a system. DNA methylation of the promoter region of ( A ) slc20a2 ; ( B ) ddx17 ; ( C ) katb6 and ( D ) xpa and relative expression of potential off-target genes ( E ) slc20a2 ; ( F ) ddx17 ; ( G ) katb6 and ( H ) xpa . Upper black lines indicate statistical difference between the blank control and sgRNA3 groups, while lines below the graph highlight that the sgRNAs were co-transfected with the CRISPR/dCas9- dnmt3a vector. The asterisks (*) are used to denote significant differences between groups using two-way ANOVA and Tukey’s multiple comparisons. *** p < 0.001.

    Article Snippet: The dnmt3a sequence and the fuw-dCas9 plasmid (Addgene #84476, Watertown, MA, USA) were digested with HamI (NEB) and EcoRI (NEB) enzymes at 37 °C for 2 h, respectively.

    Techniques: CRISPR, DNA Methylation Assay, Expressing, Control, Transfection, Plasmid Preparation

    Effects on downstream genes after the emx2 promoter region methylated. Relative expression of downstream genes ( A ) myc , ( B ) klf4 , and ( C ) wnt1 . Upper black lines indicate statistical difference among blank control and sgRNA groups for myc expression, while lines below the graph highlight that the sgRNAs were co-transfected with the CRISPR/dCas9- dnmt3a vector. The asterisks (*) are used to denote significant differences between groups using two-way ANOVA and Tukey’s multiple comparisons. *** p < 0.001.

    Journal: International Journal of Molecular Sciences

    Article Title: CRISPR/dCas9-Mediated DNA Methylation Editing on emx2 in Chinese Tongue Sole ( Cynoglossus semilaevis ) Testis Cells

    doi: 10.3390/ijms25147637

    Figure Lengend Snippet: Effects on downstream genes after the emx2 promoter region methylated. Relative expression of downstream genes ( A ) myc , ( B ) klf4 , and ( C ) wnt1 . Upper black lines indicate statistical difference among blank control and sgRNA groups for myc expression, while lines below the graph highlight that the sgRNAs were co-transfected with the CRISPR/dCas9- dnmt3a vector. The asterisks (*) are used to denote significant differences between groups using two-way ANOVA and Tukey’s multiple comparisons. *** p < 0.001.

    Article Snippet: The dnmt3a sequence and the fuw-dCas9 plasmid (Addgene #84476, Watertown, MA, USA) were digested with HamI (NEB) and EcoRI (NEB) enzymes at 37 °C for 2 h, respectively.

    Techniques: Methylation, Expressing, Control, Transfection, CRISPR, Plasmid Preparation