Sequencing:Article Title: Complete genome sequence of Anaerotignum sp. strain MB30-C6, isolated from sewage sludge of the wastewater treatment plant at a steel factory.
Article Snippet: MIGS data NCBI BioProject PRJNA1006636 NCBI BioSample SAMN37044124 GenBank accession number CP133078 Geographic location Sewage sludge of Wastewater Treatment Plant of Sanming Steel Co. Ltd., Sanming City, Fujian, China Latitude and longitude 26.2649 N, 117.6245 E Collection date 2021.06.25 Sequencing platforms DNBSEQ-T7 and Oxford Nanopore MinION Assembly method Canu v. 2.2 Coverage 858.0x Finishing strategy Complete General features of strain Classification Domain: Bacteria Phylum: Bacillota Class: Clostridia Order: Eubacteriales Family: Lachnospiraceae Genus: Anaerotignum Gram stain positive Cell shape rod-shaped Relationship to oxygen Anaerobic Optimal growth temperature 35°C Optimal growth pH 7.0 Optimal growth NaCl concentration 1.0% Genomic features Size (bp) 3,104,838 bp GC content (%) 39.49 CDSs (with protein) 2,942 Number of tRNAs 60 Number of rRNAs 7, 6, 6 (5S, 16S, 23S) April 2024 Volume 13 Issue 4 10.1128/mra.00078-24 2 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/m ra o n 11 A pr il 20 24 b y 12 4.
Article Title: Whole-genome sequencing of Aminobacterium sp. strain MB27-C1, isolated from the wastewater treatment plant of a steel mill.
Article Snippet: MIGS data NCBI BioProject PRJNA1006156 NCBI BioSample SAMN37010248 GenBank accession number CP133089 Geographic location Sewage sludge of wastewater treatment plant of Sanming Steel Co. Ltd., Sanming, Fujian, China Latitude and longitude 26.2649 N, 117.6245 E Collection date 25 June 2021 Sequencing platforms DNBSEQ-T7 and Oxford Nanopore MinION Assembly method Canu v. 2.2 Coverage 1,018.0× Finishing strategy Complete General features of strain Classification Domain: Bacteria Phylum: Synergistota Class: Synergistia Order: Synergistales Family: Synergistaceae Genus: Aminobacterium Gram stain Positive Cell shape Rod-shaped Relationship to oxygen Anaerobic Optimal growth temperature 40°C Optimal growth pH range 7 Optimal growth NaCl concentration 1.0% Genomic features Size (bp) 2,427,830 bp GC content (%) 41.58 CDSsb 2,272 Number of tRNAs 48 Number of rRNAs 3, 3, 3 (5S, 16S, 23S) antiSMASH result One RiPP-like gene clustera aRiPP, ribosomally synthesized and post-translationally modified peptide. bCDSs, Coding DNA Sequences.
Bacteria:Article Title: Complete genome sequence of Anaerotignum sp. strain MB30-C6, isolated from sewage sludge of the wastewater treatment plant at a steel factory.
Article Snippet: MIGS data NCBI BioProject PRJNA1006636 NCBI BioSample SAMN37044124 GenBank accession number CP133078 Geographic location Sewage sludge of Wastewater Treatment Plant of Sanming Steel Co. Ltd., Sanming City, Fujian, China Latitude and longitude 26.2649 N, 117.6245 E Collection date 2021.06.25 Sequencing platforms DNBSEQ-T7 and Oxford Nanopore MinION Assembly method Canu v. 2.2 Coverage 858.0x Finishing strategy Complete General features of strain Classification Domain: Bacteria Phylum: Bacillota Class: Clostridia Order: Eubacteriales Family: Lachnospiraceae Genus: Anaerotignum Gram stain positive Cell shape rod-shaped Relationship to oxygen Anaerobic Optimal growth temperature 35°C Optimal growth pH 7.0 Optimal growth NaCl concentration 1.0% Genomic features Size (bp) 3,104,838 bp GC content (%) 39.49 CDSs (with protein) 2,942 Number of tRNAs 60 Number of rRNAs 7, 6, 6 (5S, 16S, 23S) April 2024 Volume 13 Issue 4 10.1128/mra.00078-24 2 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/m ra o n 11 A pr il 20 24 b y 12 4.
Article Title: Whole-genome sequencing of Aminobacterium sp. strain MB27-C1, isolated from the wastewater treatment plant of a steel mill.
Article Snippet: MIGS data NCBI BioProject PRJNA1006156 NCBI BioSample SAMN37010248 GenBank accession number CP133089 Geographic location Sewage sludge of wastewater treatment plant of Sanming Steel Co. Ltd., Sanming, Fujian, China Latitude and longitude 26.2649 N, 117.6245 E Collection date 25 June 2021 Sequencing platforms DNBSEQ-T7 and Oxford Nanopore MinION Assembly method Canu v. 2.2 Coverage 1,018.0× Finishing strategy Complete General features of strain Classification Domain: Bacteria Phylum: Synergistota Class: Synergistia Order: Synergistales Family: Synergistaceae Genus: Aminobacterium Gram stain Positive Cell shape Rod-shaped Relationship to oxygen Anaerobic Optimal growth temperature 40°C Optimal growth pH range 7 Optimal growth NaCl concentration 1.0% Genomic features Size (bp) 2,427,830 bp GC content (%) 41.58 CDSsb 2,272 Number of tRNAs 48 Number of rRNAs 3, 3, 3 (5S, 16S, 23S) antiSMASH result One RiPP-like gene clustera aRiPP, ribosomally synthesized and post-translationally modified peptide. bCDSs, Coding DNA Sequences.
Staining:Article Title: Complete genome sequence of Anaerotignum sp. strain MB30-C6, isolated from sewage sludge of the wastewater treatment plant at a steel factory.
Article Snippet: MIGS data NCBI BioProject PRJNA1006636 NCBI BioSample SAMN37044124 GenBank accession number CP133078 Geographic location Sewage sludge of Wastewater Treatment Plant of Sanming Steel Co. Ltd., Sanming City, Fujian, China Latitude and longitude 26.2649 N, 117.6245 E Collection date 2021.06.25 Sequencing platforms DNBSEQ-T7 and Oxford Nanopore MinION Assembly method Canu v. 2.2 Coverage 858.0x Finishing strategy Complete General features of strain Classification Domain: Bacteria Phylum: Bacillota Class: Clostridia Order: Eubacteriales Family: Lachnospiraceae Genus: Anaerotignum Gram stain positive Cell shape rod-shaped Relationship to oxygen Anaerobic Optimal growth temperature 35°C Optimal growth pH 7.0 Optimal growth NaCl concentration 1.0% Genomic features Size (bp) 3,104,838 bp GC content (%) 39.49 CDSs (with protein) 2,942 Number of tRNAs 60 Number of rRNAs 7, 6, 6 (5S, 16S, 23S) April 2024 Volume 13 Issue 4 10.1128/mra.00078-24 2 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/m ra o n 11 A pr il 20 24 b y 12 4.
Article Title: Whole-genome sequencing of Aminobacterium sp. strain MB27-C1, isolated from the wastewater treatment plant of a steel mill.
Article Snippet: MIGS data NCBI BioProject PRJNA1006156 NCBI BioSample SAMN37010248 GenBank accession number CP133089 Geographic location Sewage sludge of wastewater treatment plant of Sanming Steel Co. Ltd., Sanming, Fujian, China Latitude and longitude 26.2649 N, 117.6245 E Collection date 25 June 2021 Sequencing platforms DNBSEQ-T7 and Oxford Nanopore MinION Assembly method Canu v. 2.2 Coverage 1,018.0× Finishing strategy Complete General features of strain Classification Domain: Bacteria Phylum: Synergistota Class: Synergistia Order: Synergistales Family: Synergistaceae Genus: Aminobacterium Gram stain Positive Cell shape Rod-shaped Relationship to oxygen Anaerobic Optimal growth temperature 40°C Optimal growth pH range 7 Optimal growth NaCl concentration 1.0% Genomic features Size (bp) 2,427,830 bp GC content (%) 41.58 CDSsb 2,272 Number of tRNAs 48 Number of rRNAs 3, 3, 3 (5S, 16S, 23S) antiSMASH result One RiPP-like gene clustera aRiPP, ribosomally synthesized and post-translationally modified peptide. bCDSs, Coding DNA Sequences.
Concentration Assay:Article Title: Complete genome sequence of Anaerotignum sp. strain MB30-C6, isolated from sewage sludge of the wastewater treatment plant at a steel factory.
Article Snippet: MIGS data NCBI BioProject PRJNA1006636 NCBI BioSample SAMN37044124 GenBank accession number CP133078 Geographic location Sewage sludge of Wastewater Treatment Plant of Sanming Steel Co. Ltd., Sanming City, Fujian, China Latitude and longitude 26.2649 N, 117.6245 E Collection date 2021.06.25 Sequencing platforms DNBSEQ-T7 and Oxford Nanopore MinION Assembly method Canu v. 2.2 Coverage 858.0x Finishing strategy Complete General features of strain Classification Domain: Bacteria Phylum: Bacillota Class: Clostridia Order: Eubacteriales Family: Lachnospiraceae Genus: Anaerotignum Gram stain positive Cell shape rod-shaped Relationship to oxygen Anaerobic Optimal growth temperature 35°C Optimal growth pH 7.0 Optimal growth NaCl concentration 1.0% Genomic features Size (bp) 3,104,838 bp GC content (%) 39.49 CDSs (with protein) 2,942 Number of tRNAs 60 Number of rRNAs 7, 6, 6 (5S, 16S, 23S) April 2024 Volume 13 Issue 4 10.1128/mra.00078-24 2 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/m ra o n 11 A pr il 20 24 b y 12 4.
Article Title: Whole-genome sequencing of Aminobacterium sp. strain MB27-C1, isolated from the wastewater treatment plant of a steel mill.
Article Snippet: MIGS data NCBI BioProject PRJNA1006156 NCBI BioSample SAMN37010248 GenBank accession number CP133089 Geographic location Sewage sludge of wastewater treatment plant of Sanming Steel Co. Ltd., Sanming, Fujian, China Latitude and longitude 26.2649 N, 117.6245 E Collection date 25 June 2021 Sequencing platforms DNBSEQ-T7 and Oxford Nanopore MinION Assembly method Canu v. 2.2 Coverage 1,018.0× Finishing strategy Complete General features of strain Classification Domain: Bacteria Phylum: Synergistota Class: Synergistia Order: Synergistales Family: Synergistaceae Genus: Aminobacterium Gram stain Positive Cell shape Rod-shaped Relationship to oxygen Anaerobic Optimal growth temperature 40°C Optimal growth pH range 7 Optimal growth NaCl concentration 1.0% Genomic features Size (bp) 2,427,830 bp GC content (%) 41.58 CDSsb 2,272 Number of tRNAs 48 Number of rRNAs 3, 3, 3 (5S, 16S, 23S) antiSMASH result One RiPP-like gene clustera aRiPP, ribosomally synthesized and post-translationally modified peptide. bCDSs, Coding DNA Sequences.
Synthesized:Article Title: Complete genome sequence of Anaerotignum sp. strain MB30-C6, isolated from sewage sludge of the wastewater treatment plant at a steel factory.
Article Snippet: MIGS data NCBI BioProject PRJNA1006636 NCBI BioSample SAMN37044124 GenBank accession number CP133078 Geographic location Sewage sludge of Wastewater Treatment Plant of Sanming Steel Co. Ltd., Sanming City, Fujian, China Latitude and longitude 26.2649 N, 117.6245 E Collection date 2021.06.25 Sequencing platforms DNBSEQ-T7 and Oxford Nanopore MinION Assembly method Canu v. 2.2 Coverage 858.0x Finishing strategy Complete General features of strain Classification Domain: Bacteria Phylum: Bacillota Class: Clostridia Order: Eubacteriales Family: Lachnospiraceae Genus: Anaerotignum Gram stain positive Cell shape rod-shaped Relationship to oxygen Anaerobic Optimal growth temperature 35°C Optimal growth pH 7.0 Optimal growth NaCl concentration 1.0% Genomic features Size (bp) 3,104,838 bp GC content (%) 39.49 CDSs (with protein) 2,942 Number of tRNAs 60 Number of rRNAs 7, 6, 6 (5S, 16S, 23S) April 2024 Volume 13 Issue 4 10.1128/mra.00078-24 2 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/m ra o n 11 A pr il 20 24 b y 12 4.
Article Title: Whole-genome sequencing of Aminobacterium sp. strain MB27-C1, isolated from the wastewater treatment plant of a steel mill.
Article Snippet: MIGS data NCBI BioProject PRJNA1006156 NCBI BioSample SAMN37010248 GenBank accession number CP133089 Geographic location Sewage sludge of wastewater treatment plant of Sanming Steel Co. Ltd., Sanming, Fujian, China Latitude and longitude 26.2649 N, 117.6245 E Collection date 25 June 2021 Sequencing platforms DNBSEQ-T7 and Oxford Nanopore MinION Assembly method Canu v. 2.2 Coverage 1,018.0× Finishing strategy Complete General features of strain Classification Domain: Bacteria Phylum: Synergistota Class: Synergistia Order: Synergistales Family: Synergistaceae Genus: Aminobacterium Gram stain Positive Cell shape Rod-shaped Relationship to oxygen Anaerobic Optimal growth temperature 40°C Optimal growth pH range 7 Optimal growth NaCl concentration 1.0% Genomic features Size (bp) 2,427,830 bp GC content (%) 41.58 CDSsb 2,272 Number of tRNAs 48 Number of rRNAs 3, 3, 3 (5S, 16S, 23S) antiSMASH result One RiPP-like gene clustera aRiPP, ribosomally synthesized and post-translationally modified peptide. bCDSs, Coding DNA Sequences.
Modification:Article Title: Complete genome sequence of Anaerotignum sp. strain MB30-C6, isolated from sewage sludge of the wastewater treatment plant at a steel factory.
Article Snippet: MIGS data NCBI BioProject PRJNA1006636 NCBI BioSample SAMN37044124 GenBank accession number CP133078 Geographic location Sewage sludge of Wastewater Treatment Plant of Sanming Steel Co. Ltd., Sanming City, Fujian, China Latitude and longitude 26.2649 N, 117.6245 E Collection date 2021.06.25 Sequencing platforms DNBSEQ-T7 and Oxford Nanopore MinION Assembly method Canu v. 2.2 Coverage 858.0x Finishing strategy Complete General features of strain Classification Domain: Bacteria Phylum: Bacillota Class: Clostridia Order: Eubacteriales Family: Lachnospiraceae Genus: Anaerotignum Gram stain positive Cell shape rod-shaped Relationship to oxygen Anaerobic Optimal growth temperature 35°C Optimal growth pH 7.0 Optimal growth NaCl concentration 1.0% Genomic features Size (bp) 3,104,838 bp GC content (%) 39.49 CDSs (with protein) 2,942 Number of tRNAs 60 Number of rRNAs 7, 6, 6 (5S, 16S, 23S) April 2024 Volume 13 Issue 4 10.1128/mra.00078-24 2 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/m ra o n 11 A pr il 20 24 b y 12 4.
Article Title: Whole-genome sequencing of Aminobacterium sp. strain MB27-C1, isolated from the wastewater treatment plant of a steel mill.
Article Snippet: MIGS data NCBI BioProject PRJNA1006156 NCBI BioSample SAMN37010248 GenBank accession number CP133089 Geographic location Sewage sludge of wastewater treatment plant of Sanming Steel Co. Ltd., Sanming, Fujian, China Latitude and longitude 26.2649 N, 117.6245 E Collection date 25 June 2021 Sequencing platforms DNBSEQ-T7 and Oxford Nanopore MinION Assembly method Canu v. 2.2 Coverage 1,018.0× Finishing strategy Complete General features of strain Classification Domain: Bacteria Phylum: Synergistota Class: Synergistia Order: Synergistales Family: Synergistaceae Genus: Aminobacterium Gram stain Positive Cell shape Rod-shaped Relationship to oxygen Anaerobic Optimal growth temperature 40°C Optimal growth pH range 7 Optimal growth NaCl concentration 1.0% Genomic features Size (bp) 2,427,830 bp GC content (%) 41.58 CDSsb 2,272 Number of tRNAs 48 Number of rRNAs 3, 3, 3 (5S, 16S, 23S) antiSMASH result One RiPP-like gene clustera aRiPP, ribosomally synthesized and post-translationally modified peptide. bCDSs, Coding DNA Sequences.
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