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1492r primers  (DNASTAR)


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    Structured Review

    DNASTAR 1492r primers
    1492r Primers, supplied by DNASTAR, used in various techniques. Bioz Stars score: 97/100, based on 2875 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dnastar+primer+select+program/SeqMan+Pro/pmc10715203-159-7-15
    Average 97 stars, based on 2875 article reviews
    1492r primers - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Software:

    Article Title: Molecular and Histopathological Findings of Giardia duodenalis in Rodents and Bats from the Atlantic Forest and Cerrado of Southeastern Brazil
    Article Snippet: PCR products were purified using the Wizard SV gel and PCR Clean-Up System kit (Promega, Madison, WI, USA), sequenced in both directions using the BigDye Terminator v.3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA), and run on the ABI 3730 Sequencing Platform. .. The sequences were analyzed and edited using the SeqMan software 6 (DNASTAR software package, DNASTAR Inc., Madison, WI, USA) and compared with previously published sequences using the BLASTN software from the NCBI server (http://www.ncbi.nlm.nih.gov/BLAST, accessed on 15 May 2026). .. The consensus sequences were compared with reference sequences available in the National Center for Biotechnology Information (NCBI) database using the Basic Local Alignment Search Tool (BLAST) to determine sequence similarity.

    Article Title: First description of the female of Achalinus vanhoensis Ha et al., 2022 (Squamata, Xenodermidae), with a new distribution record from northern Vietnam
    Article Snippet: Successful PCR products were purified and sequenced by Evrogen (Moscow, Russia) using an ABI 3730xl Automated DNA Analyzer (Applied Biosystems). .. Raw sequence reads were assembled and edited using SeqMan in the DNASTAR software package (Burland 2000). ..

    Article Title: Functional characterization of compound heterozygous HLCS variants reveals exon skipping and impaired biotin responsiveness in holocarboxylase synthetase deficiency.
    Article Snippet: Jo ur na l P re -p ro of Journal Pre-proof Holocarboxylase synthetase deficiency (HLCSD) is a rare inherited metabolic disorder caused by variants in the HLCS gene.. Functional characterization can aid the interpretation of rare HLCS variants.. Clinical, biochemical, genetic, transcriptional, functional, and structural analyses were performed, including urine organic acid analysis, targeted next-generation sequencing, Sanger validation, RT-PCR, cDNA sequencing, fibroblast propionyl-CoA carboxylase (PCC) activity before and after biotin treatment, and AlphaFold3-based structural modeling.

    Generated:

    Article Title: Genome sequence determination and analysis of a betabaculovirus from the moth Glena bisulca, a defoliating pest of pines.
    Article Snippet: 1 Invasive Insect Biocontrol and Behavior Laboratory, Beltsville Agricultural Research Center, USDA Agricultural Research Service, Beltsville, MD 20705, USA 2 Present address: Society for Science, 1776 Massachusetts Avenue NW, Washington, D.C. 20036, USA Abstract A baculovirus isolated from the moth Glena bisulca was characterized by electron microscopy of its occlusion bodies (OBs) and sequencing of its genome.. The OBs of this virus, Glena bisulca granulovirus isolate 1002 (GlbiGV-1002), were ovocylindrical with a single virion, as described for other betabaculoviruses.. The 104,702 bp circular genome contained six direct repeat (dr) regions and 121 open reading frames.

    DNA Sequencing:

    Article Title: Diversity of insect-specific viruses in Culex mosquitoes from Papua New Guinea.
    Article Snippet: .. Sanger sequencing data for the CO1 gene were quality-checked, assembled, and analyzed using DNASTAR Lasergene SeqMan Pro v18 and Geneious Prime v.2020.1.1 for the vertebrate host identification, when not resolved by unbiased sequencing data. ..

    Sequencing:

    Article Title: Diversity of insect-specific viruses in Culex mosquitoes from Papua New Guinea.
    Article Snippet: .. Sanger sequencing data for the CO1 gene were quality-checked, assembled, and analyzed using DNASTAR Lasergene SeqMan Pro v18 and Geneious Prime v.2020.1.1 for the vertebrate host identification, when not resolved by unbiased sequencing data. ..

    Article Title: First description of the female of Achalinus vanhoensis Ha et al., 2022 (Squamata, Xenodermidae), with a new distribution record from northern Vietnam
    Article Snippet: Successful PCR products were purified and sequenced by Evrogen (Moscow, Russia) using an ABI 3730xl Automated DNA Analyzer (Applied Biosystems). .. Raw sequence reads were assembled and edited using SeqMan in the DNASTAR software package (Burland 2000). ..

    Article Title: Functional characterization of compound heterozygous HLCS variants reveals exon skipping and impaired biotin responsiveness in holocarboxylase synthetase deficiency.
    Article Snippet: Jo ur na l P re -p ro of Journal Pre-proof Holocarboxylase synthetase deficiency (HLCSD) is a rare inherited metabolic disorder caused by variants in the HLCS gene.. Functional characterization can aid the interpretation of rare HLCS variants.. Clinical, biochemical, genetic, transcriptional, functional, and structural analyses were performed, including urine organic acid analysis, targeted next-generation sequencing, Sanger validation, RT-PCR, cDNA sequencing, fibroblast propionyl-CoA carboxylase (PCC) activity before and after biotin treatment, and AlphaFold3-based structural modeling.



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    Image Search Results


    Alignment of FLC chromosomes 8 (Ch08), 13 (Ch13), and 20 (Ch20) sequence obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Coding sequence (CDS) was obtained from Trinity‐assembled RNAseq reads (CO46 and Joelle) or from NCBI (DH55), and consensus sequence (Cons) was obtained from sequencing of PCR‐amplified cDNA. Sequence alignments were developed using the Megalign Pro application in DNASTAR Lasergene 12 software

    Journal: Plant Direct

    Article Title: Expression of FLOWERING LOCUS C and a frameshift mutation of this gene on chromosome 20 differentiate a summer and winter annual biotype of Camelina sativa

    doi: 10.1002/pld3.60

    Figure Lengend Snippet: Alignment of FLC chromosomes 8 (Ch08), 13 (Ch13), and 20 (Ch20) sequence obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Coding sequence (CDS) was obtained from Trinity‐assembled RNAseq reads (CO46 and Joelle) or from NCBI (DH55), and consensus sequence (Cons) was obtained from sequencing of PCR‐amplified cDNA. Sequence alignments were developed using the Megalign Pro application in DNASTAR Lasergene 12 software

    Article Snippet: Primers (Supporting Information Table ) were designed using the Primer Select program of DNASTAR Lasergene 12 software (DNASTAR, Inc., Madison, WI, USA) to specifically amplify cDNA based on the assembled camelina RNAseq and WGS of CO46 and Joelle.

    Techniques: Sequencing, Amplification, Software

    Pictograph of assembled whole genome sequence (WGS) and Trinity‐assembled RNAseq data (CDS) for FLC located on chromosomes 8 (Csa08), 13 (Csa13), and 20 (Csa20) obtained from the summer (CO46) or winter (Joelle) annual genotypes of Camelina sativa . Sequence alignments were developed using the Megalign Pro application in DNASTAR Lasergene 12 software. The full sequences corresponding to the pictograph can be viewed in Supporting Information Figure . The seven exons of FLC are outlined at the top of figure and lines connect the corresponding location of the MADS‐box (M), Intervening (I), keratin‐like (K), and C‐terminal (C) domains. Primer pairs used to amplify FLC for resequencing (UF2, UR2, DN47065R1, and DN24968F1) are included below the pictograph

    Journal: Plant Direct

    Article Title: Expression of FLOWERING LOCUS C and a frameshift mutation of this gene on chromosome 20 differentiate a summer and winter annual biotype of Camelina sativa

    doi: 10.1002/pld3.60

    Figure Lengend Snippet: Pictograph of assembled whole genome sequence (WGS) and Trinity‐assembled RNAseq data (CDS) for FLC located on chromosomes 8 (Csa08), 13 (Csa13), and 20 (Csa20) obtained from the summer (CO46) or winter (Joelle) annual genotypes of Camelina sativa . Sequence alignments were developed using the Megalign Pro application in DNASTAR Lasergene 12 software. The full sequences corresponding to the pictograph can be viewed in Supporting Information Figure . The seven exons of FLC are outlined at the top of figure and lines connect the corresponding location of the MADS‐box (M), Intervening (I), keratin‐like (K), and C‐terminal (C) domains. Primer pairs used to amplify FLC for resequencing (UF2, UR2, DN47065R1, and DN24968F1) are included below the pictograph

    Article Snippet: Primers (Supporting Information Table ) were designed using the Primer Select program of DNASTAR Lasergene 12 software (DNASTAR, Inc., Madison, WI, USA) to specifically amplify cDNA based on the assembled camelina RNAseq and WGS of CO46 and Joelle.

    Techniques: Sequencing, Software

    Alignment of amino acid sequence for FLC obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Sequences specific to chromosomes 8 (Ch8), 13 (Ch13), and 20 (Ch20) from the summer annual DH55 were obtained from NCBI, whereas sequence for the summer and winter annual genotypes was generated from sequence of PCR‐amplified cDNA clones. Alignments were made using the Megalign application in DNASTAR Lasergene 12 software

    Journal: Plant Direct

    Article Title: Expression of FLOWERING LOCUS C and a frameshift mutation of this gene on chromosome 20 differentiate a summer and winter annual biotype of Camelina sativa

    doi: 10.1002/pld3.60

    Figure Lengend Snippet: Alignment of amino acid sequence for FLC obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Sequences specific to chromosomes 8 (Ch8), 13 (Ch13), and 20 (Ch20) from the summer annual DH55 were obtained from NCBI, whereas sequence for the summer and winter annual genotypes was generated from sequence of PCR‐amplified cDNA clones. Alignments were made using the Megalign application in DNASTAR Lasergene 12 software

    Article Snippet: Primers (Supporting Information Table ) were designed using the Primer Select program of DNASTAR Lasergene 12 software (DNASTAR, Inc., Madison, WI, USA) to specifically amplify cDNA based on the assembled camelina RNAseq and WGS of CO46 and Joelle.

    Techniques: Sequencing, Generated, Amplification, Clone Assay, Software