qpcr primers (Microsynth ag)
90
Structured Review
Microsynth ag
qpcr primers
Qpcr Primers, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+primer+select+program/qpcr+primers/pm31323230-101-2-22
Average 90 stars, based on 1 article reviews
Qpcr Primers, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnastar+primer+select+program/qpcr+primers/pm31323230-101-2-22
Average 90 stars, based on 1 article reviews
qpcr primers - by Bioz Stars,
2026-10
90/100 stars
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Real-time Polymerase Chain Reaction:Article Title: Lapatinib Activates the Kelch-Like ECH-Associated Protein 1-Nuclear Factor Erythroid 2-Related Factor 2 Pathway in HepG2 Cells. Article Snippet: For western blotting, we used antibodies from Abcam (Cambridge, United Kingdom) and Santa Cruz Biotechnology (Heidelberg, Germany). .. The Article Title: Inhibition of Mevalonate Pathway Prevents Adipocyte Browning in Mice and Men by Affecting Protein Prenylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Fluvastatin Sigma-Aldrich Cat# SML0038 Atorvastatin Sigma-Aldrich Cat# PZ0001 Rosuvastatin Sigma-Aldrich Cat# SML1264 Pravastatin Sigma-Aldrich Cat# P4498 Cerivastatin Sigma-Aldrich Cat# SML0005 GGTI-298 Sigma-Aldrich Cat# G5169 FTI-277 Sigma-Aldrich Cat# F9803 Farnesyl pyrophosphate Sigma-Aldrich Cat# F6892 Farnesol Abcam Cat# ab142428 Geranylgeranyl pyrophosphate Sigma-Aldrich Cat# G6025 Geranylgeraniol Abcam Cat# ab142436 Gallein Santa Cruz Cat# sc-202631 Rho inhibitor, Rhosin Calbiochem Cat# 555460 Squalene Sigma-Aldrich Cat# S3626 Coenzyme Q9 Sigma-Aldrich Cat# 27597 Coenzyme Q10 Sigma-Aldrich Cat# C9538 Tamoxifen Sigma-Aldrich Cat# T5648 4-Hydroxytamoxifen Sigma-Aldrich Cat# H7904 D-glucose Sigma-Aldrich Cat# G7021 Oligomycin Adipogen Cat# 11342 Isoproterenol Sigma-Aldrich Cat# I5627 Dibutyryl-cAMP Sigma-Aldrich Cat# D0627 FCCP Sigma-Aldrich Cat# C2920 Rotenone Sigma-Aldrich Cat# R8875 Antimycin A Sigma-Aldrich Cat# A8674 Sodium pyruvate Invitrogen Cat# 11360070 Seahorse XF Base Medium Agilent Cat# 102353 Complete Protease Inhibitors Roche Cat# 05056489001 Halt Phosphatase Inhibitors ThermoFischer Cat# 78426 Click-IT Geranylgeranyl Alcohol, Azide Invitrogen Cat# C10249 Biotin Alkyne Invitrogen Cat# B10185 Phalloidin-iFluor 488 Reagent Abcam Cat# ab176753 Critical Commercial Assays High Capacity cDNA RT kit Applied Biosystems Cat# 4368814 TruSeq RNA library prep kit Illumina Cat# RS-122-2301 DC Protein Assay Bio-Rad Cat# 5000111 Click-IT Protein Reaction Kit Invitrogen Cat# C10276 XFE96 FluxPak Agilent Cat# 102416-100 Glycerol reagent Sigma-Aldrich Cat# F6428 QBT fatty acid uptake assay kit Molecular Devices Cat# R6132 Luciferase reporter system Promega Cat# E1501 Autokit Total Ketone Bodies R1 Set Wako Chemicals Cat# 415-73301 Autokit Total Ketone Bodies R2 Set Wako Chemicals Cat# 413-73601 Deposited Data RNA sequencing data for clinical transcriptome study European Nucleotide Archive PRJEB23275 Experimental Models: Cell Lines immortalized brown adipocytes Christian Wolfrum Klein et al., 2002 hMADS cells Ez-Zoubir Amri Elabd et al., 2009 (Continued on next page) e2 Cell Metabolism 29, 1–16.e1–e8, April 2, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Organisms/Strains C57BL/6N mice Charles River C57BL/6NCrl Strain code 027 Ucp1-CreERT2 x LoxP-Red mice Christian Wolfrum Rosenwald et al., 2013 Pggt1bfl/fl mice Martin Bergo Sjogren et al., 2007 Adip-CreERT2 mice Christian Wolfrum Rosenwald et al., Article Title: Allosteric Modulation of GCase Enhances Lysosomal Activity and Reduces ER Stress in GCase-Related Disorders. Article Snippet: Data analysis was performed using GraphPad Prism 5.0 Software, with values shown as mean ± SD (standard deviation) from three wells per condition. .. For quantitative polymerase chain reactions (qPCRs), the master mix was combined with Article Title: Allosteric Modulation of GCase Enhances Lysosomal Activity and Reduces ER Stress in GCase-Related Disorders Article Snippet: The isolated mRNA was reverse transcribed into cDNA using BioToolTM 2× SYBR Green qPCR Master Mix (BioTool AG, Kirchberg, Bern, Switzerland) according to the manufacturer’s instructions. .. For quantitative polymerase chain reactions (qPCRs), the master mix was combined with Article Title: Single-nucleus transcriptomics identifies separate classes of UCP1 and futile cycle adipocytes. Article Snippet: Experimental models: Cell lines hMADSC cells Ez-Zoubir Amri Elabd et al.61 293-AAV Cell Biolabs Cat# AAV-100 293-LTV Cell Biolabs Cat# LTV-100 Experimental models: Organisms/strains C57BL/6N mice Charles River C57BL/6NCrl Strain code 027 UCP1 KO mice Christian Wolfrum Enerb€ack et al.62 AdipoCre /+NucRed+/ mice Christian Wolfrum Sun et al.3 Ucp1 Dtr/egfp mice Christian Wolfrum Rosenwald et al.9 AdipoCre mice Christian Wolfrum Sun et al.3 Atp5k fl/fl mice GemPharmatech N/A Oligonucleotides qPCR primers Microsynth Listed in STAR Methods siRNAs Microsynth Listed in STAR Methods Recombinant DNA pLenti-MP2 plasmid Addgene Cat# 36097; RRID: Addgene_36097 Atp5k promoter sequences GenScript N/A pAAV-CA Addgene Cat# 69616; RRID: Addgene_69616 Software and algorithms Cellranger 10X Genomics V4.0.0 CellBender Fleming et al.63 V0.2.0 scDblFinder Pierre-Luc Germain64 V1.5.11 Seurat Hao et al.65 V4.0.4 SingleR Aran et al.66 V1.6.1 clusterProfiler Wu et al.67 V4.8.1 SUSHI Hatakeyama et al.68; Qi et al.69 N/A STAR Dobin et al.70 v2.7.4a (Continued on next page) e2 Cell Metabolism 36, 1–16.e1–e7, September 3, 2024 .. Experimental models: Cell lines hMADSC cells Ez-Zoubir Amri Elabd et al.61 293-AAV Cell Biolabs Cat# AAV-100 293-LTV Cell Biolabs Cat# LTV-100 Experimental models: Organisms/strains C57BL/6N mice Charles River C57BL/6NCrl Strain code 027 UCP1 KO mice Christian Wolfrum Enerb€ack et al.62 AdipoCre /+NucRed+/ mice Christian Wolfrum Sun et al.3 Ucp1 Dtr/egfp mice Christian Wolfrum Rosenwald et al.9 AdipoCre mice Christian Wolfrum Sun et al.3 Atp5k fl/fl Article Title: Lapatinib Activates the Kelch-Like ECH-Associated Protein 1-Nuclear Factor Erythroid 2-Related Factor 2 Pathway in HepG2 Cells Article Snippet: For western blotting, we used antibodies from Abcam (Cambridge, United Kingdom) and Santa Cruz Biotechnology (Heidelberg, Germany). .. The Produced:Article Title: Lapatinib Activates the Kelch-Like ECH-Associated Protein 1-Nuclear Factor Erythroid 2-Related Factor 2 Pathway in HepG2 Cells. Article Snippet: For western blotting, we used antibodies from Abcam (Cambridge, United Kingdom) and Santa Cruz Biotechnology (Heidelberg, Germany). .. The Article Title: Lapatinib Activates the Kelch-Like ECH-Associated Protein 1-Nuclear Factor Erythroid 2-Related Factor 2 Pathway in HepG2 Cells Article Snippet: For western blotting, we used antibodies from Abcam (Cambridge, United Kingdom) and Santa Cruz Biotechnology (Heidelberg, Germany). .. The Software:Article Title: Inhibition of Mevalonate Pathway Prevents Adipocyte Browning in Mice and Men by Affecting Protein Prenylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Fluvastatin Sigma-Aldrich Cat# SML0038 Atorvastatin Sigma-Aldrich Cat# PZ0001 Rosuvastatin Sigma-Aldrich Cat# SML1264 Pravastatin Sigma-Aldrich Cat# P4498 Cerivastatin Sigma-Aldrich Cat# SML0005 GGTI-298 Sigma-Aldrich Cat# G5169 FTI-277 Sigma-Aldrich Cat# F9803 Farnesyl pyrophosphate Sigma-Aldrich Cat# F6892 Farnesol Abcam Cat# ab142428 Geranylgeranyl pyrophosphate Sigma-Aldrich Cat# G6025 Geranylgeraniol Abcam Cat# ab142436 Gallein Santa Cruz Cat# sc-202631 Rho inhibitor, Rhosin Calbiochem Cat# 555460 Squalene Sigma-Aldrich Cat# S3626 Coenzyme Q9 Sigma-Aldrich Cat# 27597 Coenzyme Q10 Sigma-Aldrich Cat# C9538 Tamoxifen Sigma-Aldrich Cat# T5648 4-Hydroxytamoxifen Sigma-Aldrich Cat# H7904 D-glucose Sigma-Aldrich Cat# G7021 Oligomycin Adipogen Cat# 11342 Isoproterenol Sigma-Aldrich Cat# I5627 Dibutyryl-cAMP Sigma-Aldrich Cat# D0627 FCCP Sigma-Aldrich Cat# C2920 Rotenone Sigma-Aldrich Cat# R8875 Antimycin A Sigma-Aldrich Cat# A8674 Sodium pyruvate Invitrogen Cat# 11360070 Seahorse XF Base Medium Agilent Cat# 102353 Complete Protease Inhibitors Roche Cat# 05056489001 Halt Phosphatase Inhibitors ThermoFischer Cat# 78426 Click-IT Geranylgeranyl Alcohol, Azide Invitrogen Cat# C10249 Biotin Alkyne Invitrogen Cat# B10185 Phalloidin-iFluor 488 Reagent Abcam Cat# ab176753 Critical Commercial Assays High Capacity cDNA RT kit Applied Biosystems Cat# 4368814 TruSeq RNA library prep kit Illumina Cat# RS-122-2301 DC Protein Assay Bio-Rad Cat# 5000111 Click-IT Protein Reaction Kit Invitrogen Cat# C10276 XFE96 FluxPak Agilent Cat# 102416-100 Glycerol reagent Sigma-Aldrich Cat# F6428 QBT fatty acid uptake assay kit Molecular Devices Cat# R6132 Luciferase reporter system Promega Cat# E1501 Autokit Total Ketone Bodies R1 Set Wako Chemicals Cat# 415-73301 Autokit Total Ketone Bodies R2 Set Wako Chemicals Cat# 413-73601 Deposited Data RNA sequencing data for clinical transcriptome study European Nucleotide Archive PRJEB23275 Experimental Models: Cell Lines immortalized brown adipocytes Christian Wolfrum Klein et al., 2002 hMADS cells Ez-Zoubir Amri Elabd et al., 2009 (Continued on next page) e2 Cell Metabolism 29, 1–16.e1–e8, April 2, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Organisms/Strains C57BL/6N mice Charles River C57BL/6NCrl Strain code 027 Ucp1-CreERT2 x LoxP-Red mice Christian Wolfrum Rosenwald et al., 2013 Pggt1bfl/fl mice Martin Bergo Sjogren et al., 2007 Adip-CreERT2 mice Christian Wolfrum Rosenwald et al., Concentration Assay:Article Title: Allosteric Modulation of GCase Enhances Lysosomal Activity and Reduces ER Stress in GCase-Related Disorders. Article Snippet: Data analysis was performed using GraphPad Prism 5.0 Software, with values shown as mean ± SD (standard deviation) from three wells per condition. .. For quantitative polymerase chain reactions (qPCRs), the master mix was combined with Article Title: Allosteric Modulation of GCase Enhances Lysosomal Activity and Reduces ER Stress in GCase-Related Disorders Article Snippet: The isolated mRNA was reverse transcribed into cDNA using BioToolTM 2× SYBR Green qPCR Master Mix (BioTool AG, Kirchberg, Bern, Switzerland) according to the manufacturer’s instructions. .. For quantitative polymerase chain reactions (qPCRs), the master mix was combined with other:Article Title: GPR4 in the pH-dependent migration of melanoma cells in the tumor microenvironment. Article Snippet: Malignant melanoma is one of the most aggressive and deadliest forms of skin cancer, which is mainly attributed to a high metastatic potential of melanoma cells.1– 3 A central hallmark of solid tumors, such as malignant melanoma, is the acidification of the microenvironment represented by the extracellular pH (pHe).. 1,4,5 The pHe of solid tumor cells is usually more acidic (pHe 5.9– 7.0) than the extracellular environment of healthy tissue (pHe 7.3– 7.4).. 4 This is due to hypoxia, metabolic changes (Waarburg effect) and a plethora of membranebound channels/transporters (NHE1, carboanhydrases, monocarboxylate transporters, bicarbonate symporters and VATPases), which regulate pHe. Article Title: ER ‐mitochondria contacts and cholesterol metabolism are disrupted by disease‐associated tau protein Article Snippet: Bioprocessing:Article Title: Single-nucleus transcriptomics identifies separate classes of UCP1 and futile cycle adipocytes. Article Snippet: Experimental models: Cell lines hMADSC cells Ez-Zoubir Amri Elabd et al.61 293-AAV Cell Biolabs Cat# AAV-100 293-LTV Cell Biolabs Cat# LTV-100 Experimental models: Organisms/strains C57BL/6N mice Charles River C57BL/6NCrl Strain code 027 UCP1 KO mice Christian Wolfrum Enerb€ack et al.62 AdipoCre /+NucRed+/ mice Christian Wolfrum Sun et al.3 Ucp1 Dtr/egfp mice Christian Wolfrum Rosenwald et al.9 AdipoCre mice Christian Wolfrum Sun et al.3 Atp5k fl/fl mice GemPharmatech N/A Oligonucleotides qPCR primers Microsynth Listed in STAR Methods siRNAs Microsynth Listed in STAR Methods Recombinant DNA pLenti-MP2 plasmid Addgene Cat# 36097; RRID: Addgene_36097 Atp5k promoter sequences GenScript N/A pAAV-CA Addgene Cat# 69616; RRID: Addgene_69616 Software and algorithms Cellranger 10X Genomics V4.0.0 CellBender Fleming et al.63 V0.2.0 scDblFinder Pierre-Luc Germain64 V1.5.11 Seurat Hao et al.65 V4.0.4 SingleR Aran et al.66 V1.6.1 clusterProfiler Wu et al.67 V4.8.1 SUSHI Hatakeyama et al.68; Qi et al.69 N/A STAR Dobin et al.70 v2.7.4a (Continued on next page) e2 Cell Metabolism 36, 1–16.e1–e7, September 3, 2024 .. Experimental models: Cell lines hMADSC cells Ez-Zoubir Amri Elabd et al.61 293-AAV Cell Biolabs Cat# AAV-100 293-LTV Cell Biolabs Cat# LTV-100 Experimental models: Organisms/strains C57BL/6N mice Charles River C57BL/6NCrl Strain code 027 UCP1 KO mice Christian Wolfrum Enerb€ack et al.62 AdipoCre /+NucRed+/ mice Christian Wolfrum Sun et al.3 Ucp1 Dtr/egfp mice Christian Wolfrum Rosenwald et al.9 AdipoCre mice Christian Wolfrum Sun et al.3 Atp5k fl/fl Recombinant:Article Title: Single-nucleus transcriptomics identifies separate classes of UCP1 and futile cycle adipocytes. Article Snippet: Experimental models: Cell lines hMADSC cells Ez-Zoubir Amri Elabd et al.61 293-AAV Cell Biolabs Cat# AAV-100 293-LTV Cell Biolabs Cat# LTV-100 Experimental models: Organisms/strains C57BL/6N mice Charles River C57BL/6NCrl Strain code 027 UCP1 KO mice Christian Wolfrum Enerb€ack et al.62 AdipoCre /+NucRed+/ mice Christian Wolfrum Sun et al.3 Ucp1 Dtr/egfp mice Christian Wolfrum Rosenwald et al.9 AdipoCre mice Christian Wolfrum Sun et al.3 Atp5k fl/fl mice GemPharmatech N/A Oligonucleotides qPCR primers Microsynth Listed in STAR Methods siRNAs Microsynth Listed in STAR Methods Recombinant DNA pLenti-MP2 plasmid Addgene Cat# 36097; RRID: Addgene_36097 Atp5k promoter sequences GenScript N/A pAAV-CA Addgene Cat# 69616; RRID: Addgene_69616 Software and algorithms Cellranger 10X Genomics V4.0.0 CellBender Fleming et al.63 V0.2.0 scDblFinder Pierre-Luc Germain64 V1.5.11 Seurat Hao et al.65 V4.0.4 SingleR Aran et al.66 V1.6.1 clusterProfiler Wu et al.67 V4.8.1 SUSHI Hatakeyama et al.68; Qi et al.69 N/A STAR Dobin et al.70 v2.7.4a (Continued on next page) e2 Cell Metabolism 36, 1–16.e1–e7, September 3, 2024 .. Experimental models: Cell lines hMADSC cells Ez-Zoubir Amri Elabd et al.61 293-AAV Cell Biolabs Cat# AAV-100 293-LTV Cell Biolabs Cat# LTV-100 Experimental models: Organisms/strains C57BL/6N mice Charles River C57BL/6NCrl Strain code 027 UCP1 KO mice Christian Wolfrum Enerb€ack et al.62 AdipoCre /+NucRed+/ mice Christian Wolfrum Sun et al.3 Ucp1 Dtr/egfp mice Christian Wolfrum Rosenwald et al.9 AdipoCre mice Christian Wolfrum Sun et al.3 Atp5k fl/fl Plasmid Preparation:Article Title: Single-nucleus transcriptomics identifies separate classes of UCP1 and futile cycle adipocytes. Article Snippet: Experimental models: Cell lines hMADSC cells Ez-Zoubir Amri Elabd et al.61 293-AAV Cell Biolabs Cat# AAV-100 293-LTV Cell Biolabs Cat# LTV-100 Experimental models: Organisms/strains C57BL/6N mice Charles River C57BL/6NCrl Strain code 027 UCP1 KO mice Christian Wolfrum Enerb€ack et al.62 AdipoCre /+NucRed+/ mice Christian Wolfrum Sun et al.3 Ucp1 Dtr/egfp mice Christian Wolfrum Rosenwald et al.9 AdipoCre mice Christian Wolfrum Sun et al.3 Atp5k fl/fl mice GemPharmatech N/A Oligonucleotides qPCR primers Microsynth Listed in STAR Methods siRNAs Microsynth Listed in STAR Methods Recombinant DNA pLenti-MP2 plasmid Addgene Cat# 36097; RRID: Addgene_36097 Atp5k promoter sequences GenScript N/A pAAV-CA Addgene Cat# 69616; RRID: Addgene_69616 Software and algorithms Cellranger 10X Genomics V4.0.0 CellBender Fleming et al.63 V0.2.0 scDblFinder Pierre-Luc Germain64 V1.5.11 Seurat Hao et al.65 V4.0.4 SingleR Aran et al.66 V1.6.1 clusterProfiler Wu et al.67 V4.8.1 SUSHI Hatakeyama et al.68; Qi et al.69 N/A STAR Dobin et al.70 v2.7.4a (Continued on next page) e2 Cell Metabolism 36, 1–16.e1–e7, September 3, 2024 .. Experimental models: Cell lines hMADSC cells Ez-Zoubir Amri Elabd et al.61 293-AAV Cell Biolabs Cat# AAV-100 293-LTV Cell Biolabs Cat# LTV-100 Experimental models: Organisms/strains C57BL/6N mice Charles River C57BL/6NCrl Strain code 027 UCP1 KO mice Christian Wolfrum Enerb€ack et al.62 AdipoCre /+NucRed+/ mice Christian Wolfrum Sun et al.3 Ucp1 Dtr/egfp mice Christian Wolfrum Rosenwald et al.9 AdipoCre mice Christian Wolfrum Sun et al.3 Atp5k fl/fl |
