dnaman program (Gallus BioPharmaceuticals)
90
Structured Review
Gallus BioPharmaceuticals
dnaman program
miiuy (no. OP168916) and C. milii (no. XP_007887772.2). (B) FTO significantly decreased the mRNA m 6 A content. MKC cells were transfected with si-Ctrl or si-FTO and vector or FTO or FTO-mut plasmid for 48 h, then the m 6 A level was measured by colorimetry. (C) mRNA expression of FTO, IFN-1, and TNF-α in MKC cells transfected with FTO or FTO-m. (D) FTO and FTO-mut overexpressed MKC cells seeded in 48-well plates overnight were treated with SCRV at the dose indicated for 48 h. Then, cell monolayers were fixed with 4% paraformaldehyde and stained with 1% crystal violet. (E) MKC cells were transfected with pcDNA3.1 vector and FTO or FTO-mut expression plasmid for 24 h, then infected with SCRV for 24 h. The qPCR analysis was conducted for intracellular and supernatant SCRV RNA levels. (F) MKCs were transfected with pcDNA3.1 vector and FTO or FTO-mut plasmid, then infected with FITC-labeled V. anguillarum , and then examined by using a fluorescence microscope. Scale bars, 20 mm; original magnification ×400. All data presented as the means ± SE from at least three independent triplicated experiments. ∗∗, p < 0.01; ∗, p < 0.05 versus the controls. " width="250" height="auto" />
Dnaman Program, supplied by Gallus BioPharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnaman+program/dnaman+program/pmc09722488-344-17-35
Average 90 stars, based on 1 article reviews
miiuy (no. OP168916) and C. milii (no. XP_007887772.2). (B) FTO significantly decreased the mRNA m 6 A content. MKC cells were transfected with si-Ctrl or si-FTO and vector or FTO or FTO-mut plasmid for 48 h, then the m 6 A level was measured by colorimetry. (C) mRNA expression of FTO, IFN-1, and TNF-α in MKC cells transfected with FTO or FTO-m. (D) FTO and FTO-mut overexpressed MKC cells seeded in 48-well plates overnight were treated with SCRV at the dose indicated for 48 h. Then, cell monolayers were fixed with 4% paraformaldehyde and stained with 1% crystal violet. (E) MKC cells were transfected with pcDNA3.1 vector and FTO or FTO-mut expression plasmid for 24 h, then infected with SCRV for 24 h. The qPCR analysis was conducted for intracellular and supernatant SCRV RNA levels. (F) MKCs were transfected with pcDNA3.1 vector and FTO or FTO-mut plasmid, then infected with FITC-labeled V. anguillarum , and then examined by using a fluorescence microscope. Scale bars, 20 mm; original magnification ×400. All data presented as the means ± SE from at least three independent triplicated experiments. ∗∗, p < 0.01; ∗, p < 0.05 versus the controls. " width="250" height="auto" />Dnaman Program, supplied by Gallus BioPharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnaman+program/dnaman+program/pmc09722488-344-17-35
Average 90 stars, based on 1 article reviews
dnaman program - by Bioz Stars,
2026-11
90/100 stars
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1) Product Images from "FTO promotes innate immunity by controlling NOD1 expression via m 6 A-YTHDF2 manner in teleost"
Article Title: FTO promotes innate immunity by controlling NOD1 expression via m 6 A-YTHDF2 manner in teleost
Journal: iScience
doi: 10.1016/j.isci.2022.105646
Figure Legend Snippet: The RNA demethylase activity of FTO is required for promoting innate immunity (A) Sequence alignment on both sides of the mutation site "R" in FTO in H. sapiens (no. NP_001073901.1) , M. musculus (no. NP_036066.2) , G. gallus (no. NP_001172076.1) , X. laevis (no. NP_001087481.1) , D. rerio (no. XP_001345910.4) , M. miiuy (no. OP168916) and C. milii (no. XP_007887772.2). (B) FTO significantly decreased the mRNA m 6 A content. MKC cells were transfected with si-Ctrl or si-FTO and vector or FTO or FTO-mut plasmid for 48 h, then the m 6 A level was measured by colorimetry. (C) mRNA expression of FTO, IFN-1, and TNF-α in MKC cells transfected with FTO or FTO-m. (D) FTO and FTO-mut overexpressed MKC cells seeded in 48-well plates overnight were treated with SCRV at the dose indicated for 48 h. Then, cell monolayers were fixed with 4% paraformaldehyde and stained with 1% crystal violet. (E) MKC cells were transfected with pcDNA3.1 vector and FTO or FTO-mut expression plasmid for 24 h, then infected with SCRV for 24 h. The qPCR analysis was conducted for intracellular and supernatant SCRV RNA levels. (F) MKCs were transfected with pcDNA3.1 vector and FTO or FTO-mut plasmid, then infected with FITC-labeled V. anguillarum , and then examined by using a fluorescence microscope. Scale bars, 20 mm; original magnification ×400. All data presented as the means ± SE from at least three independent triplicated experiments. ∗∗, p < 0.01; ∗, p < 0.05 versus the controls.
Techniques Used: Activity Assay, Sequencing, Mutagenesis, Transfection, Plasmid Preparation, Colorimetric Assay, Expressing, Staining, Infection, Labeling, Fluorescence, Microscopy
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Activity Assay:Article Title: FTO promotes innate immunity by controlling NOD1 expression via m 6 A-YTHDF2 manner in teleost Article Snippet: Cell viability was measured at 48 h after transfection in MKC cells with CellTiter-Glo Luminescent Cell Viability assays Kit (Promega; G7570) according to the manufacturer’s instructions. .. In order to find out the site that can affect the activity of FTO demethylase in the miiuy croaker, Sequencing:Article Title: FTO promotes innate immunity by controlling NOD1 expression via m 6 A-YTHDF2 manner in teleost Article Snippet: Cell viability was measured at 48 h after transfection in MKC cells with CellTiter-Glo Luminescent Cell Viability assays Kit (Promega; G7570) according to the manufacturer’s instructions. .. In order to find out the site that can affect the activity of FTO demethylase in the miiuy croaker, |