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Sequetech corp dna sequence analysis
Dna Sequence Analysis, supplied by Sequetech corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+sequence+analysis/dna+sequence+analysis/pmc02687388-99-6-9
Average 90 stars, based on 1 article reviews
dna sequence analysis - by Bioz Stars, 2026-11
90/100 stars

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Clone Assay:

Article Title: Alpha-amylase variants
Article Snippet: .. Clones were sent to Sequetech Corporation (Mountain View, Calif.) for sequencing analysis using the following primers: 707 seq F1: (SEQ ID NO: 25) 5′ CGA TTG TGA GGA GTG GCT TGT G 3′ 707 seq R1: (SEQ ID NO: 26) 5′ CTT ATC GAT ACC GTC GAC CCT C 3′ Clones (N28R-1, S36D-4, R172Q-4, H183D-1, S255N-7, or A256T-2) were streaked on LB plates supplemented with 5 ug/mL chloramphenicol and 1% insoluble starch and grown at 37° C. overnight. ..

Article Title: Variants of an alpha-amylase with improved production levels in fermentation processes
Article Snippet: .. Clones were sent to Sequetech Corporation (Mountain View, Calif.) for sequencing analysis using the following primers: (SEQ ID NO: 25) 707 seq F1: 5′ CGA TTG TGA GGA GTG GCT TGT G 3′ (SEQ ID NO: 26) 707 seq R1: 5′ CTT ATC GAT ACC GTC GAC CCT C 3′ Clones (N28R-1, S36D-4, R172Q-4, H183D-1, S255N-7, or A256T-2) were streaked on LB plates supplemented with 5 ug/mL chloramphenicol and 1% insoluble starch and grown at 37° C. overnight. ..

Article Title: Improved Bone Morphogenetic Protein-2 Retention in an Injectable Collagen Matrix Using Bifunctional Peptides
Article Snippet: .. DNA from isolated positive phage clones was amplified using the TempliPhi DNA amplification kit and DNA analysis was performed by Sequetech (Mountain View, CA). .. DNA from isolated positive phage clones was amplified using the TempliPhi DNA amplification kit and DNA analysis was performed by Sequetech (Mountain View, CA).

Sequencing:

Article Title: Alpha-amylase variants
Article Snippet: .. Clones were sent to Sequetech Corporation (Mountain View, Calif.) for sequencing analysis using the following primers: 707 seq F1: (SEQ ID NO: 25) 5′ CGA TTG TGA GGA GTG GCT TGT G 3′ 707 seq R1: (SEQ ID NO: 26) 5′ CTT ATC GAT ACC GTC GAC CCT C 3′ Clones (N28R-1, S36D-4, R172Q-4, H183D-1, S255N-7, or A256T-2) were streaked on LB plates supplemented with 5 ug/mL chloramphenicol and 1% insoluble starch and grown at 37° C. overnight. ..

Article Title: Variants of an alpha-amylase with improved production levels in fermentation processes
Article Snippet: .. Clones were sent to Sequetech Corporation (Mountain View, Calif.) for sequencing analysis using the following primers: (SEQ ID NO: 25) 707 seq F1: 5′ CGA TTG TGA GGA GTG GCT TGT G 3′ (SEQ ID NO: 26) 707 seq R1: 5′ CTT ATC GAT ACC GTC GAC CCT C 3′ Clones (N28R-1, S36D-4, R172Q-4, H183D-1, S255N-7, or A256T-2) were streaked on LB plates supplemented with 5 ug/mL chloramphenicol and 1% insoluble starch and grown at 37° C. overnight. ..

Article Title: Probing the role of a conserved salt bridge in the intramolecular electron transfer kinetics of human sulfite oxidase.
Article Snippet: Sulfite oxidase (SO) is a vital metabolic enzyme that catalyzes the oxidation of toxic sulfite to sulfate.. The proposed mechanism of this molybdenum cofactor dependent enzyme involves two one-electron intramolecular electron transfer (IET) steps from the molybdenum center to the iron of the b5-type heme and two one-electron intermolecular electron transfer steps from the heme to cytochrome c. This work focuses on how the electrostatic interaction between two conserved amino acid residues, R472 and D342, in human SO (hSO) affects catalysis.. The hSO variants R472M, R472Q, R472K, R472D, and D342K were created to probe the effect of the position of the salt bridge charges, along with the interaction between these two residues.

Article Title: Recognition of specific sialoglycan structures by oral streptococci impacts the severity of endocardial infection
Article Snippet: To confirm the expected replacement, chromosomal DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega). .. A 4 kb region spanning 5' pdxU to gspB codon 1060 was amplified by PCR, and then subjected to DNA sequence analysis (Sequetech). ..

Article Title: Novel aspects of sialoglycan recognition by the Siglec-like domains of streptococcal SRR glycoproteins
Article Snippet: Additional replacements in Hsa BR were generated by synthesis of the entire variant BR coding region (Life Technologies). .. After cloning in the pGEX-3X expression vector, the wild-type and mutant BR coding sequences were confirmed by DNA sequence analysis (Sequetech). ..

Article Title: Kinetic Results for Mutations of Conserved Residues H304 and R309 of Human Sulfite Oxidase Point to Mechanistic Complexities
Article Snippet: Site-directed mutagenesis The mutations were introduced into the pTG918 plasmid containing the wt hSO sequence using the Quick Change Site-Directed Mutagenesis protocol (Stratagene) ( 27 ). .. The Sequetech Corporation DNA analysis facility in Mountain View, California confirmed each of the single amino acid mutations by DNA sequence analysis (see supporting information, Table S1 ). ..

Article Title: Synergistic effects on mutagenicity of tandem lesions containing 8-oxo-7,8-dihydro-2'-deoxyguanosine or Fapy•dG flanked by a 3' 5-formyl-2'-deoxyuridine in human cells.
Article Snippet: Modified nucleotides often hinder and/or decrease the fidelity of DNA polymerases.. Tandem lesions, which are comprised of DNA modifications at two contiguous nucleotide positions, can be even more detrimental to genome stability.. Recently, tandem lesions containing 5-formyl-2’-deoxyuridine (5fdU) flanked at the 5’-position by 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-OxodGuo) or N-(2-deoxy-α,β-D-erythropentofuranosyl)-N-(2,6-diamino-4-hydroxy-5-formamidopyrimidine (Fapy•dG) were discovered.

Starch:

Article Title: Alpha-amylase variants
Article Snippet: .. Clones were sent to Sequetech Corporation (Mountain View, Calif.) for sequencing analysis using the following primers: 707 seq F1: (SEQ ID NO: 25) 5′ CGA TTG TGA GGA GTG GCT TGT G 3′ 707 seq R1: (SEQ ID NO: 26) 5′ CTT ATC GAT ACC GTC GAC CCT C 3′ Clones (N28R-1, S36D-4, R172Q-4, H183D-1, S255N-7, or A256T-2) were streaked on LB plates supplemented with 5 ug/mL chloramphenicol and 1% insoluble starch and grown at 37° C. overnight. ..

Article Title: Variants of an alpha-amylase with improved production levels in fermentation processes
Article Snippet: .. Clones were sent to Sequetech Corporation (Mountain View, Calif.) for sequencing analysis using the following primers: (SEQ ID NO: 25) 707 seq F1: 5′ CGA TTG TGA GGA GTG GCT TGT G 3′ (SEQ ID NO: 26) 707 seq R1: 5′ CTT ATC GAT ACC GTC GAC CCT C 3′ Clones (N28R-1, S36D-4, R172Q-4, H183D-1, S255N-7, or A256T-2) were streaked on LB plates supplemented with 5 ug/mL chloramphenicol and 1% insoluble starch and grown at 37° C. overnight. ..

Amplification:

Article Title: Recognition of specific sialoglycan structures by oral streptococci impacts the severity of endocardial infection
Article Snippet: To confirm the expected replacement, chromosomal DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega). .. A 4 kb region spanning 5' pdxU to gspB codon 1060 was amplified by PCR, and then subjected to DNA sequence analysis (Sequetech). ..

Article Title: Improved Bone Morphogenetic Protein-2 Retention in an Injectable Collagen Matrix Using Bifunctional Peptides
Article Snippet: .. DNA from isolated positive phage clones was amplified using the TempliPhi DNA amplification kit and DNA analysis was performed by Sequetech (Mountain View, CA). .. DNA from isolated positive phage clones was amplified using the TempliPhi DNA amplification kit and DNA analysis was performed by Sequetech (Mountain View, CA).

Polymerase Chain Reaction:

Article Title: Recognition of specific sialoglycan structures by oral streptococci impacts the severity of endocardial infection
Article Snippet: To confirm the expected replacement, chromosomal DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega). .. A 4 kb region spanning 5' pdxU to gspB codon 1060 was amplified by PCR, and then subjected to DNA sequence analysis (Sequetech). ..

Cloning:

Article Title: Novel aspects of sialoglycan recognition by the Siglec-like domains of streptococcal SRR glycoproteins
Article Snippet: Additional replacements in Hsa BR were generated by synthesis of the entire variant BR coding region (Life Technologies). .. After cloning in the pGEX-3X expression vector, the wild-type and mutant BR coding sequences were confirmed by DNA sequence analysis (Sequetech). ..

Expressing:

Article Title: Novel aspects of sialoglycan recognition by the Siglec-like domains of streptococcal SRR glycoproteins
Article Snippet: Additional replacements in Hsa BR were generated by synthesis of the entire variant BR coding region (Life Technologies). .. After cloning in the pGEX-3X expression vector, the wild-type and mutant BR coding sequences were confirmed by DNA sequence analysis (Sequetech). ..

Mutagenesis:

Article Title: Novel aspects of sialoglycan recognition by the Siglec-like domains of streptococcal SRR glycoproteins
Article Snippet: Additional replacements in Hsa BR were generated by synthesis of the entire variant BR coding region (Life Technologies). .. After cloning in the pGEX-3X expression vector, the wild-type and mutant BR coding sequences were confirmed by DNA sequence analysis (Sequetech). ..

Hybridization:

Article Title: Synergistic effects on mutagenicity of tandem lesions containing 8-oxo-7,8-dihydro-2'-deoxyguanosine or Fapy•dG flanked by a 3' 5-formyl-2'-deoxyuridine in human cells.
Article Snippet: Modified nucleotides often hinder and/or decrease the fidelity of DNA polymerases.. Tandem lesions, which are comprised of DNA modifications at two contiguous nucleotide positions, can be even more detrimental to genome stability.. Recently, tandem lesions containing 5-formyl-2’-deoxyuridine (5fdU) flanked at the 5’-position by 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-OxodGuo) or N-(2-deoxy-α,β-D-erythropentofuranosyl)-N-(2,6-diamino-4-hydroxy-5-formamidopyrimidine (Fapy•dG) were discovered.

Isolation:

Article Title: Improved Bone Morphogenetic Protein-2 Retention in an Injectable Collagen Matrix Using Bifunctional Peptides
Article Snippet: .. DNA from isolated positive phage clones was amplified using the TempliPhi DNA amplification kit and DNA analysis was performed by Sequetech (Mountain View, CA). .. DNA from isolated positive phage clones was amplified using the TempliPhi DNA amplification kit and DNA analysis was performed by Sequetech (Mountain View, CA).

DNA Amplification:

Article Title: Improved Bone Morphogenetic Protein-2 Retention in an Injectable Collagen Matrix Using Bifunctional Peptides
Article Snippet: .. DNA from isolated positive phage clones was amplified using the TempliPhi DNA amplification kit and DNA analysis was performed by Sequetech (Mountain View, CA). .. DNA from isolated positive phage clones was amplified using the TempliPhi DNA amplification kit and DNA analysis was performed by Sequetech (Mountain View, CA).



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