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Fasmac Co Ltd dna sequencing, alignment, and analysis
Dna Sequencing, Alignment, And Analysis, supplied by Fasmac Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+sequence+alignment+analysis/dna+sequencing+service/pmc03781968-140-0-14
Average 90 stars, based on 1 article reviews
dna sequencing, alignment, and analysis - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Amplification:

Article Title: A rapid and simple clonality assay for bovine leukemia virus-infected cells by amplified fragment length polymorphism (AFLP) analysis
Article Snippet: .. The amplified fragments were then purified using the QIAquick PCR purification kit (QIAGEN) and Sanger sequenced by a commercial DNA sequencing service (FASMAC Co., Ltd.). .. The Sanger sequence data of the BLV integration sites were analyzed using CLOVA ( https://fasmac.co.jp/en/rais ) providing 20 nucleotide sequences of the 3’ end of BLV LTR as the transgene sequence ( ).

Article Title: PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness, photostability, and multiplexing capabilities
Article Snippet: PCR products and products of restriction digests were purified using agarose gel electrophoresis and the GeneJET gel extraction kit (Thermo Fisher Scientific). .. DNA sequences were analyzed by DNA sequence service of Fasmac Co., Ltd. To identify the initial prototypes, a portion of a tandem copy of the mScarlet gene, fused by a linker encoding GGTGGS, was amplified by PCR using three forward and three reverse primers. ..

Article Title: A rapid and simple clonality assay for bovine leukemia virus-infected cells by amplified fragment length polymorphism (AFLP) analysis.
Article Snippet: .. The amplified fragments were then purified using the QIAquick PCR purification kit (QIAGEN) and Sanger sequenced by a commercial DNA sequencing service (FASMAC Co., Ltd.). .. The Sanger sequence data of the BLV integration sites were analyzed using CLOVA (https://fasmac.co.jp/en/rais) providing 20 nucleotide sequences of the 3’ end of BLV LTR as the transgene sequence Month XXXX Volume 0 Issue 0 10.1128/spectrum.01714-2411 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/s pe ct ru m o n 24 N ov em be r 20 24 b y 24 05 :a cc 0: 12 07 :d aa :3 1e 8: a2 c3 :5 27 b: 44 e3 . (12).

Purification:

Article Title: A rapid and simple clonality assay for bovine leukemia virus-infected cells by amplified fragment length polymorphism (AFLP) analysis
Article Snippet: .. The amplified fragments were then purified using the QIAquick PCR purification kit (QIAGEN) and Sanger sequenced by a commercial DNA sequencing service (FASMAC Co., Ltd.). .. The Sanger sequence data of the BLV integration sites were analyzed using CLOVA ( https://fasmac.co.jp/en/rais ) providing 20 nucleotide sequences of the 3’ end of BLV LTR as the transgene sequence ( ).

Article Title: Evaluation of ALKBH2 and ALKBH3 gene regulation in patients with adult T-cell leukemia/lymphoma.
Article Snippet: Subsequently, TA cloning was performed using the Mighty TA-cloning kit and E. coli HST08 Premium Competent Cells (Takara Bio Inc.). .. The cloned plasmids were purified using NucleoSpin Plasmid EasyPure (Takara Bio Inc.), and sequence analysis was performed using a commercial DNA sequencing service (Fasmac Co., Ltd., Kanagawa, Japan). .. The obtained results were analyzed using GENETYX ATGC software (Genetyx Corporation, Tokyo, Japan) and QUMA, a quantification tool for methylation analysis.

Article Title: A rapid and simple clonality assay for bovine leukemia virus-infected cells by amplified fragment length polymorphism (AFLP) analysis.
Article Snippet: .. The amplified fragments were then purified using the QIAquick PCR purification kit (QIAGEN) and Sanger sequenced by a commercial DNA sequencing service (FASMAC Co., Ltd.). .. The Sanger sequence data of the BLV integration sites were analyzed using CLOVA (https://fasmac.co.jp/en/rais) providing 20 nucleotide sequences of the 3’ end of BLV LTR as the transgene sequence Month XXXX Volume 0 Issue 0 10.1128/spectrum.01714-2411 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/s pe ct ru m o n 24 N ov em be r 20 24 b y 24 05 :a cc 0: 12 07 :d aa :3 1e 8: a2 c3 :5 27 b: 44 e3 . (12).

Polymerase Chain Reaction:

Article Title: A rapid and simple clonality assay for bovine leukemia virus-infected cells by amplified fragment length polymorphism (AFLP) analysis
Article Snippet: .. The amplified fragments were then purified using the QIAquick PCR purification kit (QIAGEN) and Sanger sequenced by a commercial DNA sequencing service (FASMAC Co., Ltd.). .. The Sanger sequence data of the BLV integration sites were analyzed using CLOVA ( https://fasmac.co.jp/en/rais ) providing 20 nucleotide sequences of the 3’ end of BLV LTR as the transgene sequence ( ).

Article Title: Systematic position of edible tropical oysters and first record of genus Talonostrea (Ostreidae) in the Myeik coastal area, Myanmar
Article Snippet: .. The PCR cycling parameters were 94°C for 4 min followed by 32 cycles of 94°C for 20 s, 56°C for 40 s and 72°C for 1 min, and a final extension at 72°C for 1 min. PCR products were sequenced by Fasmac DNA sequencing service (FASMAC, Atsugi, Japan) using the same primer sets as those used for PCR. .. Each sequence result was checked for the ABI quality value (QV) using the Applied Biosystems DNA Sequencing Analysis software manual , and the base was replaced with X where QV was less than 15.

Article Title: Seasonal change and phylogenetic position of Kamegainema cingula (Nematoda: Dracunculidae) parasitic in Japanese giant salamanders
Article Snippet: .. PCR products were separated on electrophoresis gels with 1 μl Midorigreen Direct (NIPPON Genetics Co., Ltd, Tokyo, Japan), refined using the Wizard® SV Gel and PCR Clean-up System (Promega, Wasinton, U.S.A.), and sequenced directly (DNA sequencing service of FASMAC Co., Ltd., Kanagawa, Japan). ..

Article Title: PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness, photostability, and multiplexing capabilities
Article Snippet: PCR products and products of restriction digests were purified using agarose gel electrophoresis and the GeneJET gel extraction kit (Thermo Fisher Scientific). .. DNA sequences were analyzed by DNA sequence service of Fasmac Co., Ltd. To identify the initial prototypes, a portion of a tandem copy of the mScarlet gene, fused by a linker encoding GGTGGS, was amplified by PCR using three forward and three reverse primers. ..

Article Title: DNA-based floristic survey of red algae (Rhodophyta) growing in the mesophotic coral ecosystems (MCEs) offshore of Tanegashima Island, northern Ryukyu Archipelago, Japan.
Article Snippet: The amplified DNA fragments were purified using an IlluminaTM ExoProStar (Cytiva, Tokyo, Japan). .. The PCR products were sequenced by a DNA sequencing service (FASMAC, Atsugi, Japan). ..

Article Title: A rapid and simple clonality assay for bovine leukemia virus-infected cells by amplified fragment length polymorphism (AFLP) analysis.
Article Snippet: .. The amplified fragments were then purified using the QIAquick PCR purification kit (QIAGEN) and Sanger sequenced by a commercial DNA sequencing service (FASMAC Co., Ltd.). .. The Sanger sequence data of the BLV integration sites were analyzed using CLOVA (https://fasmac.co.jp/en/rais) providing 20 nucleotide sequences of the 3’ end of BLV LTR as the transgene sequence Month XXXX Volume 0 Issue 0 10.1128/spectrum.01714-2411 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/s pe ct ru m o n 24 N ov em be r 20 24 b y 24 05 :a cc 0: 12 07 :d aa :3 1e 8: a2 c3 :5 27 b: 44 e3 . (12).

DNA Sequencing:

Article Title: A rapid and simple clonality assay for bovine leukemia virus-infected cells by amplified fragment length polymorphism (AFLP) analysis
Article Snippet: .. The amplified fragments were then purified using the QIAquick PCR purification kit (QIAGEN) and Sanger sequenced by a commercial DNA sequencing service (FASMAC Co., Ltd.). .. The Sanger sequence data of the BLV integration sites were analyzed using CLOVA ( https://fasmac.co.jp/en/rais ) providing 20 nucleotide sequences of the 3’ end of BLV LTR as the transgene sequence ( ).

Article Title: Systematic position of edible tropical oysters and first record of genus Talonostrea (Ostreidae) in the Myeik coastal area, Myanmar
Article Snippet: .. The PCR cycling parameters were 94°C for 4 min followed by 32 cycles of 94°C for 20 s, 56°C for 40 s and 72°C for 1 min, and a final extension at 72°C for 1 min. PCR products were sequenced by Fasmac DNA sequencing service (FASMAC, Atsugi, Japan) using the same primer sets as those used for PCR. .. Each sequence result was checked for the ABI quality value (QV) using the Applied Biosystems DNA Sequencing Analysis software manual , and the base was replaced with X where QV was less than 15.

Article Title: Evaluation of ALKBH2 and ALKBH3 gene regulation in patients with adult T-cell leukemia/lymphoma.
Article Snippet: Subsequently, TA cloning was performed using the Mighty TA-cloning kit and E. coli HST08 Premium Competent Cells (Takara Bio Inc.). .. The cloned plasmids were purified using NucleoSpin Plasmid EasyPure (Takara Bio Inc.), and sequence analysis was performed using a commercial DNA sequencing service (Fasmac Co., Ltd., Kanagawa, Japan). .. The obtained results were analyzed using GENETYX ATGC software (Genetyx Corporation, Tokyo, Japan) and QUMA, a quantification tool for methylation analysis.

Article Title: Seasonal change and phylogenetic position of Kamegainema cingula (Nematoda: Dracunculidae) parasitic in Japanese giant salamanders
Article Snippet: .. PCR products were separated on electrophoresis gels with 1 μl Midorigreen Direct (NIPPON Genetics Co., Ltd, Tokyo, Japan), refined using the Wizard® SV Gel and PCR Clean-up System (Promega, Wasinton, U.S.A.), and sequenced directly (DNA sequencing service of FASMAC Co., Ltd., Kanagawa, Japan). ..

Article Title: DNA-based floristic survey of red algae (Rhodophyta) growing in the mesophotic coral ecosystems (MCEs) offshore of Tanegashima Island, northern Ryukyu Archipelago, Japan.
Article Snippet: The amplified DNA fragments were purified using an IlluminaTM ExoProStar (Cytiva, Tokyo, Japan). .. The PCR products were sequenced by a DNA sequencing service (FASMAC, Atsugi, Japan). ..

Article Title: Effect of Culture Supernatant of Clostridium butyricum TO-A on Human DNA-Repair-Factor-Encoding Gene Promoters
Article Snippet: .. The nucleotide sequences were confirmed by a DNA sequencing service (FASMAC, Greiner Japan Inc., Atsugi, Japan) with the primers Rv (TAGCAAAATAGGCTGTCCCC) and GL (CTTTATGTTTTTGGCGTCTTCC). ..

Article Title: A rapid and simple clonality assay for bovine leukemia virus-infected cells by amplified fragment length polymorphism (AFLP) analysis.
Article Snippet: .. The amplified fragments were then purified using the QIAquick PCR purification kit (QIAGEN) and Sanger sequenced by a commercial DNA sequencing service (FASMAC Co., Ltd.). .. The Sanger sequence data of the BLV integration sites were analyzed using CLOVA (https://fasmac.co.jp/en/rais) providing 20 nucleotide sequences of the 3’ end of BLV LTR as the transgene sequence Month XXXX Volume 0 Issue 0 10.1128/spectrum.01714-2411 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/s pe ct ru m o n 24 N ov em be r 20 24 b y 24 05 :a cc 0: 12 07 :d aa :3 1e 8: a2 c3 :5 27 b: 44 e3 . (12).

Clone Assay:

Article Title: Evaluation of ALKBH2 and ALKBH3 gene regulation in patients with adult T-cell leukemia/lymphoma.
Article Snippet: Subsequently, TA cloning was performed using the Mighty TA-cloning kit and E. coli HST08 Premium Competent Cells (Takara Bio Inc.). .. The cloned plasmids were purified using NucleoSpin Plasmid EasyPure (Takara Bio Inc.), and sequence analysis was performed using a commercial DNA sequencing service (Fasmac Co., Ltd., Kanagawa, Japan). .. The obtained results were analyzed using GENETYX ATGC software (Genetyx Corporation, Tokyo, Japan) and QUMA, a quantification tool for methylation analysis.

Plasmid Preparation:

Article Title: Evaluation of ALKBH2 and ALKBH3 gene regulation in patients with adult T-cell leukemia/lymphoma.
Article Snippet: Subsequently, TA cloning was performed using the Mighty TA-cloning kit and E. coli HST08 Premium Competent Cells (Takara Bio Inc.). .. The cloned plasmids were purified using NucleoSpin Plasmid EasyPure (Takara Bio Inc.), and sequence analysis was performed using a commercial DNA sequencing service (Fasmac Co., Ltd., Kanagawa, Japan). .. The obtained results were analyzed using GENETYX ATGC software (Genetyx Corporation, Tokyo, Japan) and QUMA, a quantification tool for methylation analysis.

Sequencing:

Article Title: Evaluation of ALKBH2 and ALKBH3 gene regulation in patients with adult T-cell leukemia/lymphoma.
Article Snippet: Subsequently, TA cloning was performed using the Mighty TA-cloning kit and E. coli HST08 Premium Competent Cells (Takara Bio Inc.). .. The cloned plasmids were purified using NucleoSpin Plasmid EasyPure (Takara Bio Inc.), and sequence analysis was performed using a commercial DNA sequencing service (Fasmac Co., Ltd., Kanagawa, Japan). .. The obtained results were analyzed using GENETYX ATGC software (Genetyx Corporation, Tokyo, Japan) and QUMA, a quantification tool for methylation analysis.

Article Title: PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness, photostability, and multiplexing capabilities
Article Snippet: PCR products and products of restriction digests were purified using agarose gel electrophoresis and the GeneJET gel extraction kit (Thermo Fisher Scientific). .. DNA sequences were analyzed by DNA sequence service of Fasmac Co., Ltd. To identify the initial prototypes, a portion of a tandem copy of the mScarlet gene, fused by a linker encoding GGTGGS, was amplified by PCR using three forward and three reverse primers. ..

Electrophoresis:

Article Title: Seasonal change and phylogenetic position of Kamegainema cingula (Nematoda: Dracunculidae) parasitic in Japanese giant salamanders
Article Snippet: .. PCR products were separated on electrophoresis gels with 1 μl Midorigreen Direct (NIPPON Genetics Co., Ltd, Tokyo, Japan), refined using the Wizard® SV Gel and PCR Clean-up System (Promega, Wasinton, U.S.A.), and sequenced directly (DNA sequencing service of FASMAC Co., Ltd., Kanagawa, Japan). ..



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