dna dapi (Santa Cruz Biotechnology)
Structured Review

Dna Dapi, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1449 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+probe+dapi/DAPI/pmc04070927-231-8-14
Average 96 stars, based on 1449 article reviews
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1) Product Images from "Cohesin without Cohesion: A Novel Role for Pds5 in Saccharomyces cerevisiae"
Article Title: Cohesin without Cohesion: A Novel Role for Pds5 in Saccharomyces cerevisiae
Journal: PLoS ONE
doi: 10.1371/journal.pone.0100470
Figure Legend Snippet: (A) Flow cytometry analyses revealing DNA content of wildtype and pds5-1 mutant cells prior to and following 3 hour incubation in nocodazole (cultures were shifted to the restrictive temperature during the final hour of incubation in medium supplemented with nocodazole). (B) Percent viability of wildtype and pds5-1 mutant cells in the presence or absence of the final shift to the restrictive temperature during mitotic arrest. (C) Percent cohesion defects of wildtype and pds5-1 mutant cells after incubation at non-permissive temperature as described in (A) above (D) Micrographs of wildtype and pds5-1 mutant cells showing separated sisters (GFP-TetR), DNA (DAPI) and retention of Pds1 indicative of a pre-anaphase state.
Techniques Used: Flow Cytometry, Mutagenesis, Incubation
Figure Legend Snippet: (A) DNA content of wildtype cells and rad61 and pds5-1 single mutant cells and pds5-1 rad61 double mutant cells as described in . (B) Percent viability of wildtype cells and rad61 and pds5-1 single mutant cells and pds5-1 rad61 double mutant cells following the regimen described in . (C) Percent of wildtype and pds5-1 mutant cells showing condensed (Lines) and uncondensed rDNA (Puffs) rDNA structures following regimen described in . (D) Micrographs of wildtype and pds5-1 mutant cells highlight rDNA structure through Net1-GFP detection (GFP) and DNA (DAPI). Pds5 inactivation specifically during S-phase impacts chromosome condensation. Wildtype and pds5-1 mutant cells were synchronized in G1 (alpha factor arrest) at permissive temperature then released to a restrictive temperature and synchronized in pre-anaphase (nocodazole arrest) prior to microscopic analysis. (E) Percent of wildtype and pds5-1 mutant cells that exhibit either condensed (Lines) or decondensed (Puffs) rDNA structures. (F) Micrographs of wildtype and pds5-1 mutant cells reveal changes in rDNA architecture.
Techniques Used: Mutagenesis
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