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dna dapi  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology dna dapi
    (A) Flow cytometry analyses revealing <t>DNA</t> content of wildtype and pds5-1 mutant cells prior to and following 3 hour incubation in nocodazole (cultures were shifted to the restrictive temperature during the final hour of incubation in medium supplemented with nocodazole). (B) Percent viability of wildtype and pds5-1 mutant cells in the presence or absence of the final shift to the restrictive temperature during mitotic arrest. (C) Percent cohesion defects of wildtype and pds5-1 mutant cells after incubation at non-permissive temperature as described in (A) above (D) Micrographs of wildtype and pds5-1 mutant cells showing separated sisters (GFP-TetR), DNA <t>(DAPI)</t> and retention of Pds1 indicative of a pre-anaphase state.
    Dna Dapi, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1449 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+probe+dapi/DAPI/pmc04070927-231-8-14
    Average 96 stars, based on 1449 article reviews
    dna dapi - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Cohesin without Cohesion: A Novel Role for Pds5 in Saccharomyces cerevisiae"

    Article Title: Cohesin without Cohesion: A Novel Role for Pds5 in Saccharomyces cerevisiae

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0100470

    (A) Flow cytometry analyses revealing DNA content of wildtype and pds5-1 mutant cells prior to and following 3 hour incubation in nocodazole (cultures were shifted to the restrictive temperature during the final hour of incubation in medium supplemented with nocodazole). (B) Percent viability of wildtype and pds5-1 mutant cells in the presence or absence of the final shift to the restrictive temperature during mitotic arrest. (C) Percent cohesion defects of wildtype and pds5-1 mutant cells after incubation at non-permissive temperature as described in (A) above (D) Micrographs of wildtype and pds5-1 mutant cells showing separated sisters (GFP-TetR), DNA (DAPI) and retention of Pds1 indicative of a pre-anaphase state.
    Figure Legend Snippet: (A) Flow cytometry analyses revealing DNA content of wildtype and pds5-1 mutant cells prior to and following 3 hour incubation in nocodazole (cultures were shifted to the restrictive temperature during the final hour of incubation in medium supplemented with nocodazole). (B) Percent viability of wildtype and pds5-1 mutant cells in the presence or absence of the final shift to the restrictive temperature during mitotic arrest. (C) Percent cohesion defects of wildtype and pds5-1 mutant cells after incubation at non-permissive temperature as described in (A) above (D) Micrographs of wildtype and pds5-1 mutant cells showing separated sisters (GFP-TetR), DNA (DAPI) and retention of Pds1 indicative of a pre-anaphase state.

    Techniques Used: Flow Cytometry, Mutagenesis, Incubation

    (A) DNA content of wildtype cells and rad61 and pds5-1 single mutant cells and pds5-1 rad61 double mutant cells as described in . (B) Percent viability of wildtype cells and rad61 and pds5-1 single mutant cells and pds5-1 rad61 double mutant cells following the regimen described in . (C) Percent of wildtype and pds5-1 mutant cells showing condensed (Lines) and uncondensed rDNA (Puffs) rDNA structures following regimen described in . (D) Micrographs of wildtype and pds5-1 mutant cells highlight rDNA structure through Net1-GFP detection (GFP) and DNA (DAPI). Pds5 inactivation specifically during S-phase impacts chromosome condensation. Wildtype and pds5-1 mutant cells were synchronized in G1 (alpha factor arrest) at permissive temperature then released to a restrictive temperature and synchronized in pre-anaphase (nocodazole arrest) prior to microscopic analysis. (E) Percent of wildtype and pds5-1 mutant cells that exhibit either condensed (Lines) or decondensed (Puffs) rDNA structures. (F) Micrographs of wildtype and pds5-1 mutant cells reveal changes in rDNA architecture.
    Figure Legend Snippet: (A) DNA content of wildtype cells and rad61 and pds5-1 single mutant cells and pds5-1 rad61 double mutant cells as described in . (B) Percent viability of wildtype cells and rad61 and pds5-1 single mutant cells and pds5-1 rad61 double mutant cells following the regimen described in . (C) Percent of wildtype and pds5-1 mutant cells showing condensed (Lines) and uncondensed rDNA (Puffs) rDNA structures following regimen described in . (D) Micrographs of wildtype and pds5-1 mutant cells highlight rDNA structure through Net1-GFP detection (GFP) and DNA (DAPI). Pds5 inactivation specifically during S-phase impacts chromosome condensation. Wildtype and pds5-1 mutant cells were synchronized in G1 (alpha factor arrest) at permissive temperature then released to a restrictive temperature and synchronized in pre-anaphase (nocodazole arrest) prior to microscopic analysis. (E) Percent of wildtype and pds5-1 mutant cells that exhibit either condensed (Lines) or decondensed (Puffs) rDNA structures. (F) Micrographs of wildtype and pds5-1 mutant cells reveal changes in rDNA architecture.

    Techniques Used: Mutagenesis

    Related Articles

    Staining:

    Article Title: Exposure of Human Corneal Epithelial Cells to Microplastic Particles Induces a Phase-Specific Cytokine Response.
    Article Snippet: 37 Microplastics have been identified in ophthalmic therapeutics and linked to dry eye 38 disease and meibomian gland dysfunction.. Inflammation is hypothesised to drive 39 ocular surface damage.. This in-vitro study investigates how varying concentrations of 40 microplastics affect corneal cell viability and cytokine expression.

    Article Title: Macrophage-derived IL-6 reprograms lipid metabolism to promote colorectal cancer development through USP14-mediated FASN deubiquitination
    Article Snippet: For immunohistochemistry, the slices were incubated with antibodies against IL-6 (Cat#AF-206-NA, R&D Systems, Minneapolis, Minnesota, USA), p-STAT3 (Cell Signaling Technology, Danvers, Massachusetts, USA), USP14 (Proteintech, Wuhan, Hubei, China) and FASN (Cat#10624-2-AP, Proteintech) and then treated with an HRP-labeled secondary antibody (Proteintech). .. After the final round of staining, DAPI (Cat#sc-3598, Santa Cruz Biotechnology, Texas, USA) solution was added. .. Photomicrographs were obtained via a light microscope (Leica, Germany).

    Article Title: Bispecific T cell engagers for treatment-refractory autoimmune connective tissue diseases.
    Article Snippet: Immunofluorescence stainings of muscle cryosections were performed with anti-CD19 (302202, 1:50; BioLegend) antibodies. .. Nuclei were stained with DAPI (sc-3598, 1:800; Santa Cruz Biotechnology). .. Stainings of skin paraffin sections were performed with the anti-BCMA (ab5972, 1:400; Abcam) antibody.

    Article Title: Glycine receptors in circulating white blood cells regulated by neuroinflammation
    Article Snippet: After incubation, the slides were washed three times with PBS for 5 minutes each, then incubated with appropriate secondary antibodies, i.e., Alexa Fluor 488 (1:200) and/or 594 (1:200) (Thermo-Fisher Scientific) for 1 hour at room temperature. .. A DAPI mounting agent (# AB104139 , Santa Cruz Biotechnology) was used for nuclear staining and mounting. ..

    Fluorescence:

    Article Title: Exposure of Human Corneal Epithelial Cells to Microplastic Particles Induces a Phase-Specific Cytokine Response.
    Article Snippet: 37 Microplastics have been identified in ophthalmic therapeutics and linked to dry eye 38 disease and meibomian gland dysfunction.. Inflammation is hypothesised to drive 39 ocular surface damage.. This in-vitro study investigates how varying concentrations of 40 microplastics affect corneal cell viability and cytokine expression.

    Microscopy:

    Article Title: Exposure of Human Corneal Epithelial Cells to Microplastic Particles Induces a Phase-Specific Cytokine Response.
    Article Snippet: 37 Microplastics have been identified in ophthalmic therapeutics and linked to dry eye 38 disease and meibomian gland dysfunction.. Inflammation is hypothesised to drive 39 ocular surface damage.. This in-vitro study investigates how varying concentrations of 40 microplastics affect corneal cell viability and cytokine expression.

    Incubation:

    Article Title: Submedius thalamic inputs shape the presynaptic architecture of layer 5 orbitofrontal cortex in mice
    Article Snippet: For quantitative analysis of trans-synaptic tracing, 40-μm coronal sections of the whole brain were collected using cryostat (model #CM1860; Leica). .. Free-floating slices were incubated at room temperature with gentle agitation as follows: 2 h in blocking solution (5% heat-inactivated goat serum, 0.4% Triton X-100 in PBS); overnight in primary antibody diluted 1:1000 in blocking solution (mouse anti-GFP, GFP-1010, Aves Labs; or anti-RFP, 5f8, Chromotek); 2–3 h in secondary antibody diluted 1:500 (donkey anti-chicken-IgY Alexa488 or goat anti-rat IgG Cy3, Jackson ImmunoResearch); and 15 min in 2.5 μg/mL of DAPI (Santa Cruz, Cat #sc-3598) in PBS. .. Sections were mounted on slides and cover-slipped with mounting medium (Fluoromount; Diagnostic BioSystems).

    Gentle:

    Article Title: Submedius thalamic inputs shape the presynaptic architecture of layer 5 orbitofrontal cortex in mice
    Article Snippet: For quantitative analysis of trans-synaptic tracing, 40-μm coronal sections of the whole brain were collected using cryostat (model #CM1860; Leica). .. Free-floating slices were incubated at room temperature with gentle agitation as follows: 2 h in blocking solution (5% heat-inactivated goat serum, 0.4% Triton X-100 in PBS); overnight in primary antibody diluted 1:1000 in blocking solution (mouse anti-GFP, GFP-1010, Aves Labs; or anti-RFP, 5f8, Chromotek); 2–3 h in secondary antibody diluted 1:500 (donkey anti-chicken-IgY Alexa488 or goat anti-rat IgG Cy3, Jackson ImmunoResearch); and 15 min in 2.5 μg/mL of DAPI (Santa Cruz, Cat #sc-3598) in PBS. .. Sections were mounted on slides and cover-slipped with mounting medium (Fluoromount; Diagnostic BioSystems).

    Blocking Assay:

    Article Title: Submedius thalamic inputs shape the presynaptic architecture of layer 5 orbitofrontal cortex in mice
    Article Snippet: For quantitative analysis of trans-synaptic tracing, 40-μm coronal sections of the whole brain were collected using cryostat (model #CM1860; Leica). .. Free-floating slices were incubated at room temperature with gentle agitation as follows: 2 h in blocking solution (5% heat-inactivated goat serum, 0.4% Triton X-100 in PBS); overnight in primary antibody diluted 1:1000 in blocking solution (mouse anti-GFP, GFP-1010, Aves Labs; or anti-RFP, 5f8, Chromotek); 2–3 h in secondary antibody diluted 1:500 (donkey anti-chicken-IgY Alexa488 or goat anti-rat IgG Cy3, Jackson ImmunoResearch); and 15 min in 2.5 μg/mL of DAPI (Santa Cruz, Cat #sc-3598) in PBS. .. Sections were mounted on slides and cover-slipped with mounting medium (Fluoromount; Diagnostic BioSystems).

    FACS:

    Article Title: Combination therapy with targeted 4-1BB (CD137) agonists/anti-FAP binding domain and anti-CEA/anti-CD3 bispecific antibody
    Article Snippet: Cells were surface-stained with fluorescent dye-conjugated antibodies anti-human CD4 (clone RPA-T4, BioLegend, Cat.-No. 300532), CD8 (clone RPa-T8, BioLegend, Cat.-No. 3010441), CD62L (clone DREG-56, BioLegend, Cat.-No. 304834), CD127 (clone 019D5, BioLegend, Cat.-No. A019D5), CD134 (clone Ber-ACT35, BioLegend, Cat.-No. 350008), CD137 (clone 4B4-1, BioLegend, Cat.-No. 309814), GITR (clone 621, BioLegend, Cat.-No. 3311608) and CD25 (clone M-A251, BioLegend, Cat.-No. 356112) for 20 min at 4° C. in FACS buffer. .. Then, they were washed once with FACS buffer before being resuspended in 85 μL/well FACS buffer containing containing 0.2 μg/mL DAPI (Santa Cruz Biotec, Cat. No. Sc-3598) before they were acquired the same day using 5-laser LSR-Fortessa (BD Bioscience with DIVA software). ..

    Software:

    Article Title: Combination therapy with targeted 4-1BB (CD137) agonists/anti-FAP binding domain and anti-CEA/anti-CD3 bispecific antibody
    Article Snippet: Cells were surface-stained with fluorescent dye-conjugated antibodies anti-human CD4 (clone RPA-T4, BioLegend, Cat.-No. 300532), CD8 (clone RPa-T8, BioLegend, Cat.-No. 3010441), CD62L (clone DREG-56, BioLegend, Cat.-No. 304834), CD127 (clone 019D5, BioLegend, Cat.-No. A019D5), CD134 (clone Ber-ACT35, BioLegend, Cat.-No. 350008), CD137 (clone 4B4-1, BioLegend, Cat.-No. 309814), GITR (clone 621, BioLegend, Cat.-No. 3311608) and CD25 (clone M-A251, BioLegend, Cat.-No. 356112) for 20 min at 4° C. in FACS buffer. .. Then, they were washed once with FACS buffer before being resuspended in 85 μL/well FACS buffer containing containing 0.2 μg/mL DAPI (Santa Cruz Biotec, Cat. No. Sc-3598) before they were acquired the same day using 5-laser LSR-Fortessa (BD Bioscience with DIVA software). ..



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    Image Search Results


    Expression and secretion of immunoregulatory mediators in cultured human MuStem cells and bone marrow-derived mesenchymal stem cells. a Flow cytometry comparison of heme oxygenase-1 (HO-1) expression in hMuStem cells and BM-MSCs. b Fluorescent immunolabeling of inducible nitric oxide synthase (iNOS) in hMuStem cells and BM-MSCs. Lipopolysaccharide (LPS)-human monocyte-derived activated macrophages for 24 h and RAW 264.7 cell line were used as positive (C+) and negative (C−) controls, respectively. Nuclei were counterstained with DAPI (blue). Scale bars, 100 µm. c Representative RT-PCR profile of indoleamine 2,3-dioxygenase-1 (IDO-1) gene obtained for hMuStem cells and BM-MSCs. For each sample, the level of IDO-1 expression was quantified using the average mRNA level of IDO-1 obtained in unstimulated BM-MSCs as a reference. LPS-human monocyte-derived activated macrophages for 24 h and water were used as positive (C+) and negative (C−) controls, respectively. d Interleukin, growth factor, enzyme and carbohydrate-binding protein secretion profile of hMuStem cells and BM-MSCs. ELISA assays were performed using culture supernatant collected 24 h after medium change. Results are expressed as individual values and normalized as concentration relative to 1 million cultured cells (ng or pg/10 6 cells). Each experiment was performed on at least 5 and 4 independent batches of hMuStem cells and BM-MSCs, respectively. Stimulation corresponds to a 24-h treatment with 50 ng/mL of TNF-α and IFN-γ. * p < 0.05, ** p < 0.01, ** p < 0.001, **** p < 0.0001; Wilcoxon matched-pairs signed rank test (unstimulated vs. stimulated) or Mann–Whitney U test (hMuStem cells vs. BM-MSCs). US, unstimulated; S, TNF-α/IFN-γ-stimulated

    Journal: Stem Cell Research & Therapy

    Article Title: Human MuStem cells repress T-cell proliferation and cytotoxicity through both paracrine and contact-dependent pathways

    doi: 10.1186/s13287-021-02681-3

    Figure Lengend Snippet: Expression and secretion of immunoregulatory mediators in cultured human MuStem cells and bone marrow-derived mesenchymal stem cells. a Flow cytometry comparison of heme oxygenase-1 (HO-1) expression in hMuStem cells and BM-MSCs. b Fluorescent immunolabeling of inducible nitric oxide synthase (iNOS) in hMuStem cells and BM-MSCs. Lipopolysaccharide (LPS)-human monocyte-derived activated macrophages for 24 h and RAW 264.7 cell line were used as positive (C+) and negative (C−) controls, respectively. Nuclei were counterstained with DAPI (blue). Scale bars, 100 µm. c Representative RT-PCR profile of indoleamine 2,3-dioxygenase-1 (IDO-1) gene obtained for hMuStem cells and BM-MSCs. For each sample, the level of IDO-1 expression was quantified using the average mRNA level of IDO-1 obtained in unstimulated BM-MSCs as a reference. LPS-human monocyte-derived activated macrophages for 24 h and water were used as positive (C+) and negative (C−) controls, respectively. d Interleukin, growth factor, enzyme and carbohydrate-binding protein secretion profile of hMuStem cells and BM-MSCs. ELISA assays were performed using culture supernatant collected 24 h after medium change. Results are expressed as individual values and normalized as concentration relative to 1 million cultured cells (ng or pg/10 6 cells). Each experiment was performed on at least 5 and 4 independent batches of hMuStem cells and BM-MSCs, respectively. Stimulation corresponds to a 24-h treatment with 50 ng/mL of TNF-α and IFN-γ. * p < 0.05, ** p < 0.01, ** p < 0.001, **** p < 0.0001; Wilcoxon matched-pairs signed rank test (unstimulated vs. stimulated) or Mann–Whitney U test (hMuStem cells vs. BM-MSCs). US, unstimulated; S, TNF-α/IFN-γ-stimulated

    Article Snippet: After incubation (1 h, RT) in blocking buffer (5% goat serum in PBS), cells were incubated overnight with iNOS Ab (1:100, sc-651 clone, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and counterstained (15 min, 37 °C) with DAPI fluorescent cell-permeable DNA probe (Life Technologies Ltd, Paisley, UK).

    Techniques: Expressing, Cell Culture, Derivative Assay, Flow Cytometry, Comparison, Immunolabeling, Reverse Transcription Polymerase Chain Reaction, Binding Assay, Enzyme-linked Immunosorbent Assay, Concentration Assay, MANN-WHITNEY

    Confocal images of O. profundus KBZ 3-2 cultured in 20 mg/L Pb for 24 h; (a) bright-field image, (b) bacterial cells stained by DAPI (blue), (c) EPS stained by Alexa Fluor 633-conjugated agglutinin (red), (d) Pb (II) stained by Leadmium Green AM Dye (green), (e) overlay image of (b–d ).

    Journal: Scientific Reports

    Article Title: Biosorption of Pb (II) and Zn (II) from aqueous solution by Oceanobacillus profundus isolated from an abandoned mine

    doi: 10.1038/s41598-020-78187-4

    Figure Lengend Snippet: Confocal images of O. profundus KBZ 3-2 cultured in 20 mg/L Pb for 24 h; (a) bright-field image, (b) bacterial cells stained by DAPI (blue), (c) EPS stained by Alexa Fluor 633-conjugated agglutinin (red), (d) Pb (II) stained by Leadmium Green AM Dye (green), (e) overlay image of (b–d ).

    Article Snippet: Three different staining dyes were sequentially added to the cell suspension to stain the DNA (DAPI Nucleic Acid Stain, Molecular probes, Invitrogen), EPS (Wheat Germ Agglutinin, Alexa Fluor 633 Conjugate, Molecular Probes, Invitrogen), and Pb (II) (Leadmium Green AM Dye, Molecular Probes, Invitrogen).

    Techniques: Cell Culture, Staining