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dna microarray hybridization oven  (Agilent technologies)


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    Agilent technologies dna microarray hybridization oven
    Dna Microarray Hybridization Oven, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+microarray+hybridization/pmc03878494-229-8-7
    Average 90 stars, based on 1 article reviews
    dna microarray hybridization oven - by Bioz Stars, 2026-11
    90/100 stars

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    other:

    Article Title: Characterization of gene expression associated with the adaptation of the nematode C. elegans to hypoxia and reoxygenation stress reveals an unexpected function of the neuroglobin GLB-5 in innate immunity.
    Article Snippet: Oxygen (O2) is a double-edged sword to cells, for while it is vital for energy production in all aerobic animals and insufficient O2 (hypoxia) can lead to cell death, the reoxygenation of hypoxic tissues may trigger the generation of reactive oxygen species (ROS) that can destroy any biological molecule.. Indeed, both hypoxia and hypoxiareoxygenation (H/R) stress are harmful, and may play a critical role in the pathophysiology of many human diseases, such as myocardial ischemia and stroke.. Therefore, understanding how animals adapt to hypoxia and H/R stress is critical for developing better treatments for these diseases.

    Article Title: A comparative analysis of the response of the hepatic transcriptome to dietary docosahexaenoic acid in Atlantic salmon ( Salmo salar ) post-smolts
    Article Snippet: Cy dye concentration and aRNA quantification was measured by Nanodrop mediated spectrophometry.

    Article Title: The peripheral blood transcriptome reflects variations in immunity traits in swine: towards the identification of biomarkers
    Article Snippet: The labelled cDNA mix was denatured at 95°C for 2 min, and 200 μl of 2X hybridization buffer (Agilent Technologies, USA) were added.

    Article Title: Distinctive gene expression profile in women with history of postpartum depression.
    Article Snippet: The Cy3 or Cy5 labeled cRNA was hybridized to the Agilent Whole Human Genome Oligo Microarray V2 (design 026652, Agilent Technologies, USA) for 17 hours at 65 °C in an Agilent DNA-Microarray Hybridization Oven.

    Microarray:

    Article Title: The Transcriptional Response to Oxidative Stress during Vertebrate Development: Effects of tert -Butylhydroquinone and 2,3,7,8-Tetrachlorodibenzo- p -Dioxin
    Article Snippet: This was followed by mixing with 2× hybridization buffer, after which 100 μl of the product was spread evenly across the surface of an Agilent 22K zebrafish microarray. .. The loaded microarray was incubated at 60°C for 17 hours with rotation in an Agilent DNA Microarray Hybridization Oven. .. Post-hybridization, microarray slides were washed, air-dried, and stored in darkness with desiccation prior to laser-excited fluorescence scanning in an Agilent DNA Microarray Scanner.

    Article Title: Neuronal Trans-differentiation in Prostate Cancer Cells
    Article Snippet: After labeling, each sample cDNA was mixed with an equal amount of labeled reference cDNA and purified with SuperScriptTM Plus Direct cDNA Labeling System (Invitrogen, Grand Island, NY) purification module according to the manufacturer's instructions. .. Eluted sample was mixed with 10X blocking solution and 2X HiRPM buffer (Agilent Technologies, Palo Alto, CA) diluted up to 100 μl of volume, then incubated for 2 min at 95-98 o C. Aliquots (95 μl) then were loaded onto the slide, and hybridized on Agilent Whole Human Genome Oligo Microarrays 4x44K (Agilent Technologies, Santa Clara, CA) using SureHyb DNA Microarray Hybridization Chambers in DNA Microarray hybridization oven (Agilent) agitated at 10 rpm, 65 o C for 20 h. After hybridization, slides were washed in Gene Expression Wash Buffer that was pre-warmed at 37 °C for 1 min and then dried by centrifugation at 2000 rpm for 2 min. Microarrays were scanned with a dynamic autofocus microarray scanner (Agilent) using Agilent-provided parameters (Red and Green PMT were each set at 100%, and scan resolution was set to 5 μm). .. The Feature Extraction Software v9.1.3.1 (Agilent) was used to extract and analyze the signals.

    Article Title: Establishment of a Novel PDX Mouse Model and Evaluation of the Tumor Suppression Efficacy of Bortezomib Against Liposarcoma
    Article Snippet: Following the labeling reaction, individually labeled test and reference samples were combined and concentrated using Amicon Ultra-0.5 centrifugal filters (Millipore, Billerica, MA). .. After denaturing the probe and preannealing it to human Cot-1 DNA, samples were hybridized at 65°C and 20 rpm rotation for 24 hours in a DNA Microarray Hybridization Oven (Agilent Technologies). .. Samples were washed in wash buffer 1 at room temperature for 5 minutes and wash buffer 2 at 37°C for 1 minute using Agilent Oligo CGH washes.

    Article Title: Nutrigenomic profiling of transcriptional processes affected in liver and distal intestine in response to a soybean meal-induced nutritional stress in Atlantic salmon (Salmo salar).
    Article Snippet: .. For each reaction, 825 ng Cy5 labelled reference pool and 825 ng of the individual Cy3 labelled samples were processed with the Gene Expression Hybridization Kit (Agilent Technologies) following the manufacturer’s instructions, applied to the arrays and immediately incubated using SureHyb hybridization chambers in a DNA Microarray Hybridization Oven (Agilent Technologies) at 65 °C for 17 h. Throughout the experiment samples were randomized, preventing samples from the same treatment being overrepresented in a particular batch in order to avoid unintentional biases. .. Scanning was performed using a GenePix 4200 AL Scanner (Molecular Devices (UK) Ltd., Wokingham, UK) and the resulting images analyzed with Agilent Feature Extraction Software v.9.5 (Agilent Technologies) to extract the intensity values and identify the features.

    Incubation:

    Article Title: The Transcriptional Response to Oxidative Stress during Vertebrate Development: Effects of tert -Butylhydroquinone and 2,3,7,8-Tetrachlorodibenzo- p -Dioxin
    Article Snippet: This was followed by mixing with 2× hybridization buffer, after which 100 μl of the product was spread evenly across the surface of an Agilent 22K zebrafish microarray. .. The loaded microarray was incubated at 60°C for 17 hours with rotation in an Agilent DNA Microarray Hybridization Oven. .. Post-hybridization, microarray slides were washed, air-dried, and stored in darkness with desiccation prior to laser-excited fluorescence scanning in an Agilent DNA Microarray Scanner.

    Article Title: Neuronal Trans-differentiation in Prostate Cancer Cells
    Article Snippet: After labeling, each sample cDNA was mixed with an equal amount of labeled reference cDNA and purified with SuperScriptTM Plus Direct cDNA Labeling System (Invitrogen, Grand Island, NY) purification module according to the manufacturer's instructions. .. Eluted sample was mixed with 10X blocking solution and 2X HiRPM buffer (Agilent Technologies, Palo Alto, CA) diluted up to 100 μl of volume, then incubated for 2 min at 95-98 o C. Aliquots (95 μl) then were loaded onto the slide, and hybridized on Agilent Whole Human Genome Oligo Microarrays 4x44K (Agilent Technologies, Santa Clara, CA) using SureHyb DNA Microarray Hybridization Chambers in DNA Microarray hybridization oven (Agilent) agitated at 10 rpm, 65 o C for 20 h. After hybridization, slides were washed in Gene Expression Wash Buffer that was pre-warmed at 37 °C for 1 min and then dried by centrifugation at 2000 rpm for 2 min. Microarrays were scanned with a dynamic autofocus microarray scanner (Agilent) using Agilent-provided parameters (Red and Green PMT were each set at 100%, and scan resolution was set to 5 μm). .. The Feature Extraction Software v9.1.3.1 (Agilent) was used to extract and analyze the signals.

    Article Title: Nutrigenomic profiling of transcriptional processes affected in liver and distal intestine in response to a soybean meal-induced nutritional stress in Atlantic salmon (Salmo salar).
    Article Snippet: .. For each reaction, 825 ng Cy5 labelled reference pool and 825 ng of the individual Cy3 labelled samples were processed with the Gene Expression Hybridization Kit (Agilent Technologies) following the manufacturer’s instructions, applied to the arrays and immediately incubated using SureHyb hybridization chambers in a DNA Microarray Hybridization Oven (Agilent Technologies) at 65 °C for 17 h. Throughout the experiment samples were randomized, preventing samples from the same treatment being overrepresented in a particular batch in order to avoid unintentional biases. .. Scanning was performed using a GenePix 4200 AL Scanner (Molecular Devices (UK) Ltd., Wokingham, UK) and the resulting images analyzed with Agilent Feature Extraction Software v.9.5 (Agilent Technologies) to extract the intensity values and identify the features.

    Hybridization:

    Article Title: The Transcriptional Response to Oxidative Stress during Vertebrate Development: Effects of tert -Butylhydroquinone and 2,3,7,8-Tetrachlorodibenzo- p -Dioxin
    Article Snippet: This was followed by mixing with 2× hybridization buffer, after which 100 μl of the product was spread evenly across the surface of an Agilent 22K zebrafish microarray. .. The loaded microarray was incubated at 60°C for 17 hours with rotation in an Agilent DNA Microarray Hybridization Oven. .. Post-hybridization, microarray slides were washed, air-dried, and stored in darkness with desiccation prior to laser-excited fluorescence scanning in an Agilent DNA Microarray Scanner.

    Article Title: Neuronal Trans-differentiation in Prostate Cancer Cells
    Article Snippet: After labeling, each sample cDNA was mixed with an equal amount of labeled reference cDNA and purified with SuperScriptTM Plus Direct cDNA Labeling System (Invitrogen, Grand Island, NY) purification module according to the manufacturer's instructions. .. Eluted sample was mixed with 10X blocking solution and 2X HiRPM buffer (Agilent Technologies, Palo Alto, CA) diluted up to 100 μl of volume, then incubated for 2 min at 95-98 o C. Aliquots (95 μl) then were loaded onto the slide, and hybridized on Agilent Whole Human Genome Oligo Microarrays 4x44K (Agilent Technologies, Santa Clara, CA) using SureHyb DNA Microarray Hybridization Chambers in DNA Microarray hybridization oven (Agilent) agitated at 10 rpm, 65 o C for 20 h. After hybridization, slides were washed in Gene Expression Wash Buffer that was pre-warmed at 37 °C for 1 min and then dried by centrifugation at 2000 rpm for 2 min. Microarrays were scanned with a dynamic autofocus microarray scanner (Agilent) using Agilent-provided parameters (Red and Green PMT were each set at 100%, and scan resolution was set to 5 μm). .. The Feature Extraction Software v9.1.3.1 (Agilent) was used to extract and analyze the signals.

    Article Title: Establishment of a Novel PDX Mouse Model and Evaluation of the Tumor Suppression Efficacy of Bortezomib Against Liposarcoma
    Article Snippet: Following the labeling reaction, individually labeled test and reference samples were combined and concentrated using Amicon Ultra-0.5 centrifugal filters (Millipore, Billerica, MA). .. After denaturing the probe and preannealing it to human Cot-1 DNA, samples were hybridized at 65°C and 20 rpm rotation for 24 hours in a DNA Microarray Hybridization Oven (Agilent Technologies). .. Samples were washed in wash buffer 1 at room temperature for 5 minutes and wash buffer 2 at 37°C for 1 minute using Agilent Oligo CGH washes.

    Article Title: Nutrigenomic profiling of transcriptional processes affected in liver and distal intestine in response to a soybean meal-induced nutritional stress in Atlantic salmon (Salmo salar).
    Article Snippet: .. For each reaction, 825 ng Cy5 labelled reference pool and 825 ng of the individual Cy3 labelled samples were processed with the Gene Expression Hybridization Kit (Agilent Technologies) following the manufacturer’s instructions, applied to the arrays and immediately incubated using SureHyb hybridization chambers in a DNA Microarray Hybridization Oven (Agilent Technologies) at 65 °C for 17 h. Throughout the experiment samples were randomized, preventing samples from the same treatment being overrepresented in a particular batch in order to avoid unintentional biases. .. Scanning was performed using a GenePix 4200 AL Scanner (Molecular Devices (UK) Ltd., Wokingham, UK) and the resulting images analyzed with Agilent Feature Extraction Software v.9.5 (Agilent Technologies) to extract the intensity values and identify the features.

    Blocking Assay:

    Article Title: Neuronal Trans-differentiation in Prostate Cancer Cells
    Article Snippet: After labeling, each sample cDNA was mixed with an equal amount of labeled reference cDNA and purified with SuperScriptTM Plus Direct cDNA Labeling System (Invitrogen, Grand Island, NY) purification module according to the manufacturer's instructions. .. Eluted sample was mixed with 10X blocking solution and 2X HiRPM buffer (Agilent Technologies, Palo Alto, CA) diluted up to 100 μl of volume, then incubated for 2 min at 95-98 o C. Aliquots (95 μl) then were loaded onto the slide, and hybridized on Agilent Whole Human Genome Oligo Microarrays 4x44K (Agilent Technologies, Santa Clara, CA) using SureHyb DNA Microarray Hybridization Chambers in DNA Microarray hybridization oven (Agilent) agitated at 10 rpm, 65 o C for 20 h. After hybridization, slides were washed in Gene Expression Wash Buffer that was pre-warmed at 37 °C for 1 min and then dried by centrifugation at 2000 rpm for 2 min. Microarrays were scanned with a dynamic autofocus microarray scanner (Agilent) using Agilent-provided parameters (Red and Green PMT were each set at 100%, and scan resolution was set to 5 μm). .. The Feature Extraction Software v9.1.3.1 (Agilent) was used to extract and analyze the signals.

    Gene Expression:

    Article Title: Neuronal Trans-differentiation in Prostate Cancer Cells
    Article Snippet: After labeling, each sample cDNA was mixed with an equal amount of labeled reference cDNA and purified with SuperScriptTM Plus Direct cDNA Labeling System (Invitrogen, Grand Island, NY) purification module according to the manufacturer's instructions. .. Eluted sample was mixed with 10X blocking solution and 2X HiRPM buffer (Agilent Technologies, Palo Alto, CA) diluted up to 100 μl of volume, then incubated for 2 min at 95-98 o C. Aliquots (95 μl) then were loaded onto the slide, and hybridized on Agilent Whole Human Genome Oligo Microarrays 4x44K (Agilent Technologies, Santa Clara, CA) using SureHyb DNA Microarray Hybridization Chambers in DNA Microarray hybridization oven (Agilent) agitated at 10 rpm, 65 o C for 20 h. After hybridization, slides were washed in Gene Expression Wash Buffer that was pre-warmed at 37 °C for 1 min and then dried by centrifugation at 2000 rpm for 2 min. Microarrays were scanned with a dynamic autofocus microarray scanner (Agilent) using Agilent-provided parameters (Red and Green PMT were each set at 100%, and scan resolution was set to 5 μm). .. The Feature Extraction Software v9.1.3.1 (Agilent) was used to extract and analyze the signals.

    Article Title: Nutrigenomic profiling of transcriptional processes affected in liver and distal intestine in response to a soybean meal-induced nutritional stress in Atlantic salmon (Salmo salar).
    Article Snippet: .. For each reaction, 825 ng Cy5 labelled reference pool and 825 ng of the individual Cy3 labelled samples were processed with the Gene Expression Hybridization Kit (Agilent Technologies) following the manufacturer’s instructions, applied to the arrays and immediately incubated using SureHyb hybridization chambers in a DNA Microarray Hybridization Oven (Agilent Technologies) at 65 °C for 17 h. Throughout the experiment samples were randomized, preventing samples from the same treatment being overrepresented in a particular batch in order to avoid unintentional biases. .. Scanning was performed using a GenePix 4200 AL Scanner (Molecular Devices (UK) Ltd., Wokingham, UK) and the resulting images analyzed with Agilent Feature Extraction Software v.9.5 (Agilent Technologies) to extract the intensity values and identify the features.

    Centrifugation:

    Article Title: Neuronal Trans-differentiation in Prostate Cancer Cells
    Article Snippet: After labeling, each sample cDNA was mixed with an equal amount of labeled reference cDNA and purified with SuperScriptTM Plus Direct cDNA Labeling System (Invitrogen, Grand Island, NY) purification module according to the manufacturer's instructions. .. Eluted sample was mixed with 10X blocking solution and 2X HiRPM buffer (Agilent Technologies, Palo Alto, CA) diluted up to 100 μl of volume, then incubated for 2 min at 95-98 o C. Aliquots (95 μl) then were loaded onto the slide, and hybridized on Agilent Whole Human Genome Oligo Microarrays 4x44K (Agilent Technologies, Santa Clara, CA) using SureHyb DNA Microarray Hybridization Chambers in DNA Microarray hybridization oven (Agilent) agitated at 10 rpm, 65 o C for 20 h. After hybridization, slides were washed in Gene Expression Wash Buffer that was pre-warmed at 37 °C for 1 min and then dried by centrifugation at 2000 rpm for 2 min. Microarrays were scanned with a dynamic autofocus microarray scanner (Agilent) using Agilent-provided parameters (Red and Green PMT were each set at 100%, and scan resolution was set to 5 μm). .. The Feature Extraction Software v9.1.3.1 (Agilent) was used to extract and analyze the signals.



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