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oligonucleotide microarray hybridization  (Genisphere llc)

 
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    Genisphere llc oligonucleotide microarray hybridization
    Oligonucleotide Microarray Hybridization, supplied by Genisphere llc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+microarray+chip+method/pmc01756913-40-6-9?v=Genisphere+llc
    Average 90 stars, based on 1 article reviews
    oligonucleotide microarray hybridization - by Bioz Stars, 2026-08
    90/100 stars

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    The layout of the DNA microarray method module; each detection panel includes 4 detection modules, which can detect two specimens at the same time. Modules 1 and 3 are used to detect mutations in the rpoB gene, and modules 2 and 4 are used to detect mutations in the katG gene and inhA promoter. QC quality control probe; EC external control probe; BC blank control; NC negative control probe; IC internal control probe; WT wild-type.

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: The layout of the DNA microarray method module; each detection panel includes 4 detection modules, which can detect two specimens at the same time. Modules 1 and 3 are used to detect mutations in the rpoB gene, and modules 2 and 4 are used to detect mutations in the katG gene and inhA promoter. QC quality control probe; EC external control probe; BC blank control; NC negative control probe; IC internal control probe; WT wild-type.

    Article Snippet: The CapitalBio DNA microarray chip method is used to qualitatively detect nucleic acids in samples of Mtb isolates from clinical TB patients.

    Techniques: Microarray, Negative Control

    The drug susceptibility test was used as a standard method to evaluate the efficacy of the DNA microarray for detecting RIF and INH resistance and MDR-TB.

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: The drug susceptibility test was used as a standard method to evaluate the efficacy of the DNA microarray for detecting RIF and INH resistance and MDR-TB.

    Article Snippet: The CapitalBio DNA microarray chip method is used to qualitatively detect nucleic acids in samples of Mtb isolates from clinical TB patients.

    Techniques: Microarray

    Comparison of the diagnostic efficacy of the DNA microarray method when sputum smear grades were ≤ 1 + and ≥ 2 +

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: Comparison of the diagnostic efficacy of the DNA microarray method when sputum smear grades were ≤ 1 + and ≥ 2 +

    Article Snippet: The CapitalBio DNA microarray chip method is used to qualitatively detect nucleic acids in samples of Mtb isolates from clinical TB patients.

    Techniques: Diagnostic Assay, Microarray

    Specimen processing procedure: A total of 5,911 sputum smear-positive specimens were collected. After experimental processing, 4148 specimens that were positive with the DNA microarray method and DST were finally included in the study. NTM, nontuberculous mycobacteria ; DST, drug sensitivity test.

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: Specimen processing procedure: A total of 5,911 sputum smear-positive specimens were collected. After experimental processing, 4148 specimens that were positive with the DNA microarray method and DST were finally included in the study. NTM, nontuberculous mycobacteria ; DST, drug sensitivity test.

    Article Snippet: The CapitalBio DNA microarray chip method is used to qualitatively detect nucleic acids in samples of Mtb isolates from clinical TB patients.

    Techniques: Microarray

    Pattern diagrams of several common drug-resistant gene mutations detected by the DNA microarray method. The white box is the detection site of the wild-type codon, and the red box is the site of the detected mutant codon. ( a ) rpoB gene Leu511Pro (CTG → CCG); ( b ) rpoB gene Asp516Tyr (GAC → TAC); ( c ) rpoB gene His526Tyr (CAC → TAC); ( d ) rpoB gene Ser531Trp (TCG → TGG); ( e ) rpoB gene Ser531Leu (TCG → TTG); ( f ) katG gene Ser315Thr (AGC → ACC); ( g ) katG gene Ser315Asn (AGC → AAC); ( h ) inhA gene promoter-15 (C → T).

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: Pattern diagrams of several common drug-resistant gene mutations detected by the DNA microarray method. The white box is the detection site of the wild-type codon, and the red box is the site of the detected mutant codon. ( a ) rpoB gene Leu511Pro (CTG → CCG); ( b ) rpoB gene Asp516Tyr (GAC → TAC); ( c ) rpoB gene His526Tyr (CAC → TAC); ( d ) rpoB gene Ser531Trp (TCG → TGG); ( e ) rpoB gene Ser531Leu (TCG → TTG); ( f ) katG gene Ser315Thr (AGC → ACC); ( g ) katG gene Ser315Asn (AGC → AAC); ( h ) inhA gene promoter-15 (C → T).

    Article Snippet: The CapitalBio DNA microarray chip method is used to qualitatively detect nucleic acids in samples of Mtb isolates from clinical TB patients.

    Techniques: Microarray, Mutagenesis

    Non-tuberculous species identified from 2013 to 2018 in a tertiary hospital in Beijing, China

    Journal: BMC Microbiology

    Article Title: Prevalence of nontuberculous mycobacteria in a tertiary hospital in Beijing, China, January 2013 to December 2018

    doi: 10.1186/s12866-020-01840-5

    Figure Lengend Snippet: Non-tuberculous species identified from 2013 to 2018 in a tertiary hospital in Beijing, China

    Article Snippet: DNA microarray chip method (Mycobacterial Species Identification Array Kit, CapitalBio Technology Inc., Beijing, China) can accurately distinguish between M. avium and M. intracellulae , which have quite similar phenotypes.

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    Specimen types among which NTM were identified in this study

    Journal: BMC Microbiology

    Article Title: Prevalence of nontuberculous mycobacteria in a tertiary hospital in Beijing, China, January 2013 to December 2018

    doi: 10.1186/s12866-020-01840-5

    Figure Lengend Snippet: Specimen types among which NTM were identified in this study

    Article Snippet: DNA microarray chip method (Mycobacterial Species Identification Array Kit, CapitalBio Technology Inc., Beijing, China) can accurately distinguish between M. avium and M. intracellulae , which have quite similar phenotypes.

    Techniques: