dna barcoding  (Bio-Rad)

Journal: Current Issues in Molecular Biology

Article Title: From “Omics” to Field: Deciphering the Stress Adaptation Networks and Breeding Potential of Medicago ruthenica L.

doi: 10.3390/cimb48040365

Figure Lengend Snippet: Workflow of Medicago ruthenica genome sequencing, created in BioRender. Tao, T. (2026) https://BioRender.com/pftnl2s .

Article Snippet: In 2023, comparison of multiple M. ruthenica nuclear genomic DNA barcodes revealed that the single-copy gene GA3ox1 achieved 100% identification accuracy when distinguishing M. ruthenica from its cryptic relative Medicago archiducis - nicolai [ ].

Techniques: Sequencing

Journal: Current Issues in Molecular Biology

Article Title: From “Omics” to Field: Deciphering the Stress Adaptation Networks and Breeding Potential of Medicago ruthenica L.

doi: 10.3390/cimb48040365

Figure Lengend Snippet: Schematic representation of the multi-dimensional stress response mechanisms in Medicago ruthenica . The diagram illustrates the physiological, biochemical, and molecular adaptations of M. ruthenica under four major abiotic stresses: () Drought Stress (top left), characterized by the regulation of stomatal movement, osmotic adjustment via proline and soluble sugars, and the activation of DREB/ERF transcription factors; () Temperature Stress (top right), involving membrane lipid remodeling, antioxidant enzyme (SOD, POD) activation, and the expression of cold-responsive genes (e.g., MrCOR , MrCBF ); () Salt Stress (bottom left), highlighting ion homeostasis maintenance (Na + /K + balance) and the synthesis of osmoprotectants; and () Heavy Metal Stress (bottom right), depicting chelation, sequestration, and detoxification processes. The central panel emphasizes the role of beneficial root-associated microorganisms (e.g., Rhizobium , arbuscular mycorrhizal fungi (AMF)) in enhancing systemic stress tolerance through nitrogen fixation, nutrient solubilization, and the modulation of phytohormone signaling. Created in BioRender. Tao, T. (2026) https://BioRender.com/6ekbej8 .

Article Snippet: In 2023, comparison of multiple M. ruthenica nuclear genomic DNA barcodes revealed that the single-copy gene GA3ox1 achieved 100% identification accuracy when distinguishing M. ruthenica from its cryptic relative Medicago archiducis - nicolai [ ].

Techniques: Activation Assay, Membrane, Expressing