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amplification procedure  (TaKaRa)


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    Structured Review

    TaKaRa amplification procedure
    Amplification Procedure, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 16130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+amplification+methods/TaKaRa+Ex+Taq+DNA+Polymerase/pm39577832-65-1-15
    Average 98 stars, based on 16130 article reviews
    amplification procedure - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Mitochondrial genetic diversity and population structure of Glyphoglossus guttulatus Blyth, 1856 (Anura: Microhylidae) across the Thai Lower Mekong Basin and adjacent northern drainage systems
    Article Snippet: The Burmese squat frog, Glyphoglossus guttulatus Blyth, 1856, is a widespread fossorial microhylid distributed throughout mainland Southeast Asia, yet its population genetic structure in Thailand remains poorly understood.. We investigated mitochondrial genetic diversity and population structure using partial cytochrome c oxidase subunit I (COI) sequences from 116 individuals representing 11 populations across northern and northeastern Thailand, with emphasis on the Thai Lower Mekong Basin.. High haplotype diversity (Hd = 0.942 ± 0.009) and moderate nucleotide diversity (π = 0.02904 ± 0.00153) were detected.

    Article Title: Morphological and immunohistochemical analysis of unilateral testicular hypoplasia in an XY calf: correlation with the timing of bovine hypospadias.
    Article Snippet: .. The primer sequences were as follows: Sry: Forward 5'-AAGCGACCCATGAATGCAT TGATGGTGTGGT-3' Reverse 5'-GAGGTCGATACTTATAGTTCGGGTATTTCTCTCTGTG-3' Amelogenin: Forward 5'-CAGCCAAACCTCCCTCTGC-3' Reverse 5'-CCCGCTTGGTCTTGTCTGTTGC-3' Cytochrome b: Forward 5'- AAAAAGCTTCCATCCAACATCTCAGCATGATGAAA-3' Reverse 5'- AAACTGCAGCCCCTCAGAATGATATTTGTCCTCA-3' All PCR amplifications were performed using Ex Taq DNA Polymerase (Takara Bio, Kusatsu, Japan) in a total volume of 20 μL. ..

    Article Title: Nu class glutathione S-transferase (GST) from the freshwater diatom Fragilaria saxoplanctonica: characterization and transcriptional responses to pollutant-induced oxidative stress
    Article Snippet: Background Diatoms are important primary producers in aquatic ecosystems and are continuously affected by pollutants that disturb cellular redox homeostasis.. Glutathione S-transferases (GSTs) are involved in glutathione-dependent detoxification and redox regulation; however, information regarding GSTs class specific characteristics and pollutant-responsive expression patterns in diatoms remains limited.. Objectives We identified a novel Nu class GST, designated as FsGSTNu, from the freshwater diatom Fragilaria saxoplanctonica and examined its transcriptional responses under exposures of six metals (NaAsO2, CdSO4, K2Cr2O7, CuSO4, NiSO4 and ZnSO4) and three pesticides (atrazine, chlorpyrifos, and S-metolachlor).

    Article Title: Vascular-specific FAM43B inhibits the Wnt pathway by regulating β-catenin ubiquitination and alleviates the progression of atherosclerosis.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Transcription start site aware catalogue of microRNA encoded peptides in tomato (Solanum lycopersicum)
    Article Snippet: .. PCR amplification was performed using Ex-Taq polymerase (TAKARA) in 20 μL reactions containing 1× buffer, 0.2 mM dNTPs, 0.2 μM of each primer, and 1 μL cDNA template. ..

    Amplification:

    Article Title: Diversity of Ascetosporeans Associated With Feather Hydroids in New Caledonia
    Article Snippet: DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen) according to the manufacturer's protocol. .. For species identification of feather hydroids, the 18S rRNA gene was amplified from each sample using the primer set 18SF and 18SR (Yabuki et al. ) and Ex Taq DNA polymerase (Takara) under the following conditions: an initial denaturation at 96°C for 1 min, followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and extension at 72°C for 2 min, with a final hold at 4°C. ..

    Article Title: Nu class glutathione S-transferase (GST) from the freshwater diatom Fragilaria saxoplanctonica: characterization and transcriptional responses to pollutant-induced oxidative stress
    Article Snippet: Background Diatoms are important primary producers in aquatic ecosystems and are continuously affected by pollutants that disturb cellular redox homeostasis.. Glutathione S-transferases (GSTs) are involved in glutathione-dependent detoxification and redox regulation; however, information regarding GSTs class specific characteristics and pollutant-responsive expression patterns in diatoms remains limited.. Objectives We identified a novel Nu class GST, designated as FsGSTNu, from the freshwater diatom Fragilaria saxoplanctonica and examined its transcriptional responses under exposures of six metals (NaAsO2, CdSO4, K2Cr2O7, CuSO4, NiSO4 and ZnSO4) and three pesticides (atrazine, chlorpyrifos, and S-metolachlor).

    Article Title: Vascular-specific FAM43B inhibits the Wnt pathway by regulating β-catenin ubiquitination and alleviates the progression of atherosclerosis.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Transcription start site aware catalogue of microRNA encoded peptides in tomato (Solanum lycopersicum)
    Article Snippet: .. PCR amplification was performed using Ex-Taq polymerase (TAKARA) in 20 μL reactions containing 1× buffer, 0.2 mM dNTPs, 0.2 μM of each primer, and 1 μL cDNA template. ..

    Sequencing:

    Article Title: Nu class glutathione S-transferase (GST) from the freshwater diatom Fragilaria saxoplanctonica: characterization and transcriptional responses to pollutant-induced oxidative stress
    Article Snippet: Background Diatoms are important primary producers in aquatic ecosystems and are continuously affected by pollutants that disturb cellular redox homeostasis.. Glutathione S-transferases (GSTs) are involved in glutathione-dependent detoxification and redox regulation; however, information regarding GSTs class specific characteristics and pollutant-responsive expression patterns in diatoms remains limited.. Objectives We identified a novel Nu class GST, designated as FsGSTNu, from the freshwater diatom Fragilaria saxoplanctonica and examined its transcriptional responses under exposures of six metals (NaAsO2, CdSO4, K2Cr2O7, CuSO4, NiSO4 and ZnSO4) and three pesticides (atrazine, chlorpyrifos, and S-metolachlor).

    Genotyping:

    Article Title: Vascular-specific FAM43B inhibits the Wnt pathway by regulating β-catenin ubiquitination and alleviates the progression of atherosclerosis.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.



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    Image Search Results


    Fluorescence emission from the microfluidic device following 40 minutes of S. equi DNA LAMP amplification. There is equal fluorescence at 40 minutes whether LAMP detects 1 CFU or 100 CFU. CFU, colony forming unit; LAMP, loop‐mediated isothermal nucleic acid amplification

    Journal: Journal of Veterinary Internal Medicine

    Article Title: Detection of Streptococcus equi subsp. equi in guttural pouch lavage samples using a loop‐mediated isothermal nucleic acid amplification microfluidic device

    doi: 10.1111/jvim.16105

    Figure Lengend Snippet: Fluorescence emission from the microfluidic device following 40 minutes of S. equi DNA LAMP amplification. There is equal fluorescence at 40 minutes whether LAMP detects 1 CFU or 100 CFU. CFU, colony forming unit; LAMP, loop‐mediated isothermal nucleic acid amplification

    Article Snippet: Logistic regression power analysis determined a sample size of 60 GPL specimens were enough for the study to demonstrate a difference between the 3 DNA amplification methods (power of 80% and α ≤ .05) (STATA 14.0).

    Techniques: Fluorescence, Amplification

    (A) Fluorescent by‐product of LAMP distinguished a positive vs negative result of S. equi DNA detection on dry storage microfluidic device (B) as seen via a smartphone screen. LAMP, loop‐mediated isothermal nucleic acid amplification

    Journal: Journal of Veterinary Internal Medicine

    Article Title: Detection of Streptococcus equi subsp. equi in guttural pouch lavage samples using a loop‐mediated isothermal nucleic acid amplification microfluidic device

    doi: 10.1111/jvim.16105

    Figure Lengend Snippet: (A) Fluorescent by‐product of LAMP distinguished a positive vs negative result of S. equi DNA detection on dry storage microfluidic device (B) as seen via a smartphone screen. LAMP, loop‐mediated isothermal nucleic acid amplification

    Article Snippet: Logistic regression power analysis determined a sample size of 60 GPL specimens were enough for the study to demonstrate a difference between the 3 DNA amplification methods (power of 80% and α ≤ .05) (STATA 14.0).

    Techniques: Amplification