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mouse igg1 y45  (ACROBiosystems)


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    Structured Review

    ACROBiosystems mouse igg1 y45

    Mouse Igg1 Y45, supplied by ACROBiosystems, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dmf/PE-Labeled+Monoclonal+Anti-FMC63+scFv+Antibody%2C+Mouse+IgG1+(Y45)+(Site-specific+conjugation)+DMF+Filed/pmc09826863-2-5-9
    Average 95 stars, based on 6 article reviews
    mouse igg1 y45 - by Bioz Stars, 2026-11
    95/100 stars

    Images

    1) Product Images from "Inducing T cell dysfunction by chronic stimulation of CAR-engineered T cells targeting cancer cells in suspension cultures"

    Article Title: Inducing T cell dysfunction by chronic stimulation of CAR-engineered T cells targeting cancer cells in suspension cultures

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2022.101954


    Figure Legend Snippet:

    Techniques Used: Recombinant, Reverse Transcription, Software, Pore Size, Flow Cytometry, Transfection

    Related Articles

    other:

    Article Title: Inducing T cell dysfunction by chronic stimulation of CAR-engineered T cells targeting cancer cells in suspension cultures
    Article Snippet: PE-labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) , Acro Biosystems , Cat# FM3-HPY53; RRID: AB_2921284.

    FACS:

    Article Title: CD19/CD20 dual-targeted chimeric antigen receptor-engineered natural killer cells exhibit improved cytotoxicity against acute lymphoblastic leukemia
    Article Snippet: To assess the cell surface expression of CAR, electroporated cells were stained with Biotinylated Human CD20 Full-Length protein (stock 50 μg/mL, 1:25 dilution, ACRO Biosystems, Beijing, CHN) for 60 min at 4 °C. .. This was followed by two washes in FACS buffer (PBA solution with 2% bovine serum albumin) and subsequent staining with allophycocyanin (APC)-conjugated Streptavidin Protein (stock: 200 μg/mL, 1:200 dilution, ACRO Biosystems) and FITC-Labeled Monoclonal Anti-FMC63 Antibody (stock: 50 μL, 1:50 dilution, ACRO Biosystems, clone: Y45) (or PE-Labeled Monoclonal Anti-FMC63 Antibody (stock: 50 μL, 1:50 dilution, ACRO Biosystems, clone: Y45)) for 60 min at 4 °C. ..

    Article Title: CD19/CD20 dual-targeted chimeric antigen receptor-engineered natural killer cells exhibit improved cytotoxicity against acute lymphoblastic leukemia.
    Article Snippet: To assess the cell surface expression of CAR, electroporated cells were stained with Biotinylated Human CD20 Full-Length protein (stock 50 μg/mL, 1:25 dilution, ACRO Biosystems, Beijing, CHN) for 60 min at 4 °C. .. This was followed by two washes in FACS buffer (PBA solution with 2% bovine serum albumin) and subsequent staining with allophycocyanin (APC)-conjugated Streptavidin Protein (stock: 200 μg/mL, 1:200 dilution, ACRO Biosystems) and FITC-Labeled Monoclonal Anti-FMC63 Antibody (stock: 50 μL, 1:50 dilution, ACRO Biosystems, clone: Y45) (or PE-Labeled Monoclonal Anti-FMC63 Antibody (stock: 50 μL, 1:50 dilution, ACRO Biosystems, clone: Y45)) for 60 min at 4 °C. ..

    Staining:

    Article Title: CD19/CD20 dual-targeted chimeric antigen receptor-engineered natural killer cells exhibit improved cytotoxicity against acute lymphoblastic leukemia
    Article Snippet: To assess the cell surface expression of CAR, electroporated cells were stained with Biotinylated Human CD20 Full-Length protein (stock 50 μg/mL, 1:25 dilution, ACRO Biosystems, Beijing, CHN) for 60 min at 4 °C. .. This was followed by two washes in FACS buffer (PBA solution with 2% bovine serum albumin) and subsequent staining with allophycocyanin (APC)-conjugated Streptavidin Protein (stock: 200 μg/mL, 1:200 dilution, ACRO Biosystems) and FITC-Labeled Monoclonal Anti-FMC63 Antibody (stock: 50 μL, 1:50 dilution, ACRO Biosystems, clone: Y45) (or PE-Labeled Monoclonal Anti-FMC63 Antibody (stock: 50 μL, 1:50 dilution, ACRO Biosystems, clone: Y45)) for 60 min at 4 °C. ..

    Article Title: Targeting HLA-DR loss in hematologic malignancies with an inhibitory chimeric antigen receptor
    Article Snippet: Data were analyzed with FlowJo software (TreeStar). .. Different CAR-NK cells were stained with PE-conjugated anti-FMC63 scFv antibody (ACRO Biosystems, DE, USA) and anti-FLAG-tag antibody (BioLegend) at saturating concentrations, respectively. .. Cells were analyzed by flow cytometry, and data were analyzed using FlowJo software.

    Article Title: CD19/CD20 dual-targeted chimeric antigen receptor-engineered natural killer cells exhibit improved cytotoxicity against acute lymphoblastic leukemia.
    Article Snippet: To assess the cell surface expression of CAR, electroporated cells were stained with Biotinylated Human CD20 Full-Length protein (stock 50 μg/mL, 1:25 dilution, ACRO Biosystems, Beijing, CHN) for 60 min at 4 °C. .. This was followed by two washes in FACS buffer (PBA solution with 2% bovine serum albumin) and subsequent staining with allophycocyanin (APC)-conjugated Streptavidin Protein (stock: 200 μg/mL, 1:200 dilution, ACRO Biosystems) and FITC-Labeled Monoclonal Anti-FMC63 Antibody (stock: 50 μL, 1:50 dilution, ACRO Biosystems, clone: Y45) (or PE-Labeled Monoclonal Anti-FMC63 Antibody (stock: 50 μL, 1:50 dilution, ACRO Biosystems, clone: Y45)) for 60 min at 4 °C. ..

    Flow Cytometry:

    Article Title: Cytokine co-presentation on targeted lipid nanoparticles enhances in vivo T cell engineering
    Article Snippet: .. The following flow cytometry reagents were used: Live/Dead Fixable Near IR (Invitrogen), Live/Dead Fixable Aqua (Invitrogen), Alexa Fluor 488 anti-mouse CD3 (clone 17A2, BioLegend), FITC anti-mouse CD44 (clone NIM-R8, BioLegend), APC anti-mouse CD3 (clone 17A2, BioLegend), APC anti-mouse CD19 (clone 6D5, BioLegend), APC anti-mouse CD20 (clone SA275A11, BioLegend), APC-Cy7 anti-mouse CD8a (clone 53–6.7, BioLegend), Brilliant Violet 421 anti-mouse CD4 (clone GK1.5, BioLegend), Brilliant Violet 421 anti-human CD69 (clone FN50, BioLegend), Brilliant Violet 605 anti-mouse CD8a (clone 53–6.7, BioLegend), Brilliant Violet 605 anti-mouse CD62L (clone MEL-14, BioLegend), Brilliant Violet 785 anti-mouse CD45 (clone 30-F11, BioLegend), PE anti-FMC63 (clone Y45, ACROBiosystems). ..

    Expressing:

    Article Title: Generation of anti-tumor chimeric antigen receptors incorporating T cell signaling motifs
    Article Snippet: Cells were washed in FACS buffer (PBS supplemented with 2% BSA), Fc blocking was performed with a combination of antibodies diluted in FACS buffer with Brilliant Violet Stain Buffer (BD Biosciences) for 30 minutes at 4°C in the dark. .. Surface expression of CD19-CAR was detected with PE-anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) (Acro Biosystems FM3-HPY53). .. The following antibodies were used on cells isolated from bleeds and spleen/bone marrow of NSG treated mice: CD3 APC R700 (BD 565119), CD4 BB700 (BD 566392), CD8 APC Cy7 (BioLegend 344714), PD1 PE-Cy7 (BioLegend 329918), Tim3 AF647 (BD 565558), LAG3 BV605 (BioLegend 369324), CD62L BV650 (BD 583808), CD45RA BV786 (BD 565419).



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