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human cd19  (ACROBiosystems)


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    Structured Review

    ACROBiosystems human cd19
    a , b Percentages of CD62L + CD45RA + cells within CD4 + ( a ) and CD8 + ( b ) populations of 28z or 28z-hNELFB CAR-T cells after 10 days of in vitro expansion, n = 4/group. c Survival curves of Raji tumor-bearing mice receiving PBS or mock T cells, <t>CD19</t> CAR-28z T cells, or CD19 CAR-28z-hNELFB T cells. d Model of enhancer–promoter looping mediated by NELFB, Pol II, and TCF1. Mean differences were compared using Student’s t -test. Log-rank (Mantel–Cox) tests were used for survival analyses. Source data are provided as a Source Data file.
    Human Cd19, supplied by ACROBiosystems, used in various techniques. Bioz Stars score: 95/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dmf/FITC-Labeled+Human+CD19+(20-291)+Protein%2C+His+Tag+DMF+Filed/pmc09021285-331-8-10
    Average 95 stars, based on 11 article reviews
    human cd19 - by Bioz Stars, 2026-11
    95/100 stars

    Images

    1) Product Images from "RNA polymerase II pausing factor NELF in CD8 + T cells promotes antitumor immunity"

    Article Title: RNA polymerase II pausing factor NELF in CD8 + T cells promotes antitumor immunity

    Journal: Nature Communications

    doi: 10.1038/s41467-022-29869-2

    a , b Percentages of CD62L + CD45RA + cells within CD4 + ( a ) and CD8 + ( b ) populations of 28z or 28z-hNELFB CAR-T cells after 10 days of in vitro expansion, n = 4/group. c Survival curves of Raji tumor-bearing mice receiving PBS or mock T cells, CD19 CAR-28z T cells, or CD19 CAR-28z-hNELFB T cells. d Model of enhancer–promoter looping mediated by NELFB, Pol II, and TCF1. Mean differences were compared using Student’s t -test. Log-rank (Mantel–Cox) tests were used for survival analyses. Source data are provided as a Source Data file.
    Figure Legend Snippet: a , b Percentages of CD62L + CD45RA + cells within CD4 + ( a ) and CD8 + ( b ) populations of 28z or 28z-hNELFB CAR-T cells after 10 days of in vitro expansion, n = 4/group. c Survival curves of Raji tumor-bearing mice receiving PBS or mock T cells, CD19 CAR-28z T cells, or CD19 CAR-28z-hNELFB T cells. d Model of enhancer–promoter looping mediated by NELFB, Pol II, and TCF1. Mean differences were compared using Student’s t -test. Log-rank (Mantel–Cox) tests were used for survival analyses. Source data are provided as a Source Data file.

    Techniques Used: In Vitro

    Related Articles

    Expressing:

    Article Title: Rapid generation of CD19 CAR-T Cells by minicircle DNA enables anti-tumor activity and prevents fatal CAR-B leukemia
    Article Snippet: The CD8+ T cell phenotypic and its rate change before and after the sorting process were analyzed by flow cytometry with CD8-FITC (eBioscience). .. The CD19 CAR expression on the surface of CAR-T cells was measured FITC-Labeled Human CD19 (20-291) Protein (ACRO biosystem, China). ..

    Article Title: Rapid generation of CD19 CAR-T cells by minicircle DNA enables anti-tumor activity and prevents fatal CAR-B leukemia.
    Article Snippet: .. After lentivirus transduction for at least 72 h or electroporation for 48 h, CAR expression efficiencies and the intensity of CAR expression in transfected T cells were detected by flow cytometry using FITC-labeled human CD19 (20–291) protein(ACRO Biosystem, China). .. To generate MC-based CD19-CAR-Ball-1 and CD19-CAR-Raji cells, Ball-1 or Raji cells were transduced with mcDNA-CD19-CAR using the preset program DS104 of the 4D-NucleofectorTM system (Lonza).

    Article Title: Rapid generation of CD19 CAR-T Cells by minicircle DNA enables anti-tumor activity and prevents fatal CAR-B leukemia
    Article Snippet: .. After lentivirus transduction for at least 72 h or electroporation for 48 h, the CAR expression efficiencies, and the intensity of CAR expression of transfected T cells were detected by flow cytometry using FITC-Labeled Human CD19 (20-291) Protein(ACRO biosystem, China). .. For generation of the MC-based CD19-CAR-Raji, the Raji cells were transduced with mcDNA-CD19-CAR by using preset program DS104 of 4D-NucleofectorTM system (Lonza).

    Article Title: Rapid generation of CD19 CAR-T cells by minicircle DNA enables anti-tumor activity and prevents fatal CAR-B leukemia.
    Article Snippet: The CD8+ T cell phenotype and its rate of change before and after sorting were analyzed by flow cytometry using CD8-FITC (eBioscience). .. The CD19 CAR expression rate and mean fluorescence intensity on the surface of CAR-T cells were measured using FITC-labeled human CD19 (20–291) protein (ACRO Biosystem, China). ..

    BIA-KA:

    Article Title: Identification and analysis of microplastic aggregation in CAR-T cells.
    Article Snippet: Microplastics (MPs) are increasingly recognized as contaminants present in various environments and are widely acknowledged as potential hazards to the mammalian immune system.. In our study of chimeric antigen receptor T cell (CAR-T) therapy, we observed the presence of MP in CAR-T cell products for the first time.. It is worth exploring whether MP could enter CAR-T cells and how they might affect CAR-T cells’ functionality.

    Transduction:

    Article Title: Rapid generation of CD19 CAR-T cells by minicircle DNA enables anti-tumor activity and prevents fatal CAR-B leukemia.
    Article Snippet: .. After lentivirus transduction for at least 72 h or electroporation for 48 h, CAR expression efficiencies and the intensity of CAR expression in transfected T cells were detected by flow cytometry using FITC-labeled human CD19 (20–291) protein(ACRO Biosystem, China). .. To generate MC-based CD19-CAR-Ball-1 and CD19-CAR-Raji cells, Ball-1 or Raji cells were transduced with mcDNA-CD19-CAR using the preset program DS104 of the 4D-NucleofectorTM system (Lonza).

    Article Title: Rapid generation of CD19 CAR-T Cells by minicircle DNA enables anti-tumor activity and prevents fatal CAR-B leukemia
    Article Snippet: .. After lentivirus transduction for at least 72 h or electroporation for 48 h, the CAR expression efficiencies, and the intensity of CAR expression of transfected T cells were detected by flow cytometry using FITC-Labeled Human CD19 (20-291) Protein(ACRO biosystem, China). .. For generation of the MC-based CD19-CAR-Raji, the Raji cells were transduced with mcDNA-CD19-CAR by using preset program DS104 of 4D-NucleofectorTM system (Lonza).

    Electroporation:

    Article Title: Rapid generation of CD19 CAR-T cells by minicircle DNA enables anti-tumor activity and prevents fatal CAR-B leukemia.
    Article Snippet: .. After lentivirus transduction for at least 72 h or electroporation for 48 h, CAR expression efficiencies and the intensity of CAR expression in transfected T cells were detected by flow cytometry using FITC-labeled human CD19 (20–291) protein(ACRO Biosystem, China). .. To generate MC-based CD19-CAR-Ball-1 and CD19-CAR-Raji cells, Ball-1 or Raji cells were transduced with mcDNA-CD19-CAR using the preset program DS104 of the 4D-NucleofectorTM system (Lonza).

    Article Title: Rapid generation of CD19 CAR-T Cells by minicircle DNA enables anti-tumor activity and prevents fatal CAR-B leukemia
    Article Snippet: .. After lentivirus transduction for at least 72 h or electroporation for 48 h, the CAR expression efficiencies, and the intensity of CAR expression of transfected T cells were detected by flow cytometry using FITC-Labeled Human CD19 (20-291) Protein(ACRO biosystem, China). .. For generation of the MC-based CD19-CAR-Raji, the Raji cells were transduced with mcDNA-CD19-CAR by using preset program DS104 of 4D-NucleofectorTM system (Lonza).

    Transfection:

    Article Title: Rapid generation of CD19 CAR-T cells by minicircle DNA enables anti-tumor activity and prevents fatal CAR-B leukemia.
    Article Snippet: .. After lentivirus transduction for at least 72 h or electroporation for 48 h, CAR expression efficiencies and the intensity of CAR expression in transfected T cells were detected by flow cytometry using FITC-labeled human CD19 (20–291) protein(ACRO Biosystem, China). .. To generate MC-based CD19-CAR-Ball-1 and CD19-CAR-Raji cells, Ball-1 or Raji cells were transduced with mcDNA-CD19-CAR using the preset program DS104 of the 4D-NucleofectorTM system (Lonza).

    Article Title: Rapid generation of CD19 CAR-T Cells by minicircle DNA enables anti-tumor activity and prevents fatal CAR-B leukemia
    Article Snippet: .. After lentivirus transduction for at least 72 h or electroporation for 48 h, the CAR expression efficiencies, and the intensity of CAR expression of transfected T cells were detected by flow cytometry using FITC-Labeled Human CD19 (20-291) Protein(ACRO biosystem, China). .. For generation of the MC-based CD19-CAR-Raji, the Raji cells were transduced with mcDNA-CD19-CAR by using preset program DS104 of 4D-NucleofectorTM system (Lonza).

    Flow Cytometry:

    Article Title: Rapid generation of CD19 CAR-T cells by minicircle DNA enables anti-tumor activity and prevents fatal CAR-B leukemia.
    Article Snippet: .. After lentivirus transduction for at least 72 h or electroporation for 48 h, CAR expression efficiencies and the intensity of CAR expression in transfected T cells were detected by flow cytometry using FITC-labeled human CD19 (20–291) protein(ACRO Biosystem, China). .. To generate MC-based CD19-CAR-Ball-1 and CD19-CAR-Raji cells, Ball-1 or Raji cells were transduced with mcDNA-CD19-CAR using the preset program DS104 of the 4D-NucleofectorTM system (Lonza).

    Article Title: Rapid generation of CD19 CAR-T Cells by minicircle DNA enables anti-tumor activity and prevents fatal CAR-B leukemia
    Article Snippet: .. After lentivirus transduction for at least 72 h or electroporation for 48 h, the CAR expression efficiencies, and the intensity of CAR expression of transfected T cells were detected by flow cytometry using FITC-Labeled Human CD19 (20-291) Protein(ACRO biosystem, China). .. For generation of the MC-based CD19-CAR-Raji, the Raji cells were transduced with mcDNA-CD19-CAR by using preset program DS104 of 4D-NucleofectorTM system (Lonza).

    Article Title: Donor-derived CARCIK-CD19 cells engineered with Sleeping Beauty transposon in acute lymphoblastic leukemia relapsed after allogeneic transplantation
    Article Snippet: .. Flow cytometry was used to perform pharmacokinetic analysis using the following antibody: human CD19 (20-291) protein, his-tag (Acro Biosystems; CD9-H52H2 4 ), Anti his APC (clone GG11-8F3.5.1 Miltenyi Biotec; 130-119-782), Anti CD3 FITC (clone SK7, BD Bioscience; 345763), Anti CD45 V500-C (clone 2D1, BD Bioscience; 655873). ..

    Fluorescence:

    Article Title: Rapid generation of CD19 CAR-T cells by minicircle DNA enables anti-tumor activity and prevents fatal CAR-B leukemia.
    Article Snippet: The CD8+ T cell phenotype and its rate of change before and after sorting were analyzed by flow cytometry using CD8-FITC (eBioscience). .. The CD19 CAR expression rate and mean fluorescence intensity on the surface of CAR-T cells were measured using FITC-labeled human CD19 (20–291) protein (ACRO Biosystem, China). ..

    Staining:

    Article Title: Impact of the kinetics of circulating anti-CD19 CAR-T cells and their populations on the outcome of DLBCL patients
    Article Snippet: .. For this purpose, 10 mL of fresh (<12h) blood was collected in K3-EDTA Vacutainer tubes -Becton/Dickinson Biosciences (BD), New Jersey, NJ- per time point per patient, aliquoted, and stained with the EuroFlow Lymphocyte Screening Tube (LST) (5), and both the immune monitoring (IMM) T CD4+ (TCD4) and T/NK-cell cytotoxic (Cytox) tubes, to which the CD19 protein (20-291)-FITC reagent from ACROBiosystems (Bethesda, MD) was added. ..

    Article Title: The Clinical Significance of Circulating Lymphocytes Morphology in Diffuse Large B-Cell Lymphoma As Determined by a Novel, Highly Sensitive Microscopy
    Article Snippet: .. The cells were stained with the combination of 10 μg/mL of FITC-labeled human CD19 (20-291) protein Fc-Tag (Acrobiosystems Inc., Newark, DE, USA) and CD3-APCH7 (BD Biosciences, San Diego, CA, USA). .. Samples were acquired by FACS Canto II and analyzed by DIVA software (BD Biosciences, San Diego, CA, USA).



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