Injection:Article Title: Preparation of cleared whole-mount urethra and urinary bladder from transcardially perfused mice for immunolabeling and analysis by CLSM.
Article Snippet: .. Dissecting scissors, medium FST 14184–09 Dissecting scissors, fine 14 cm straight FST 15020–15 Forceps, fine FST 11252–50 Forceps, coarse FST 11992–12 Clamp (artery clamp) FST 13018–14 TAIJI-IE Small Animal Anesthesia Machine RWD TAIJI-IE Infusion bottle (1000 mL, clear, Stopper Infusion 32 mm) or Infusion bottle, for example Braun’s isotonic sodium chloride solution 0.9% Packsys Braun 10000263 PZN:08646871/ EAN4030539036313 Glass-tubing cutter Merck Z150770-1EA Pravaz needle with LL mount, blunt version, size 1 diameter 0.9 mm at least 38 mm length or Laboratory pipetting needle with 90 ◦ blunt ends, gauge 20, L 4 in., 304 stainless steel hub Acufirm Merck 1400 LL CAD7950-12EA Scienceware® trays; L 3 W 3 H 30 cm 3 41 cm 3 7.6 cm Merck Z503797-1EA Stainless steel perforated sheet, square hole 10 mm - thickness 2.00 mm; L 3 W 31 cm 3 42 cm Elbemetal www.elbemetall.de Intrafix® SafeSet with LL mount 180 cm with 3 3 4.1 mm tubing Braun PZN 01900697/EAN 4022495869517 Syring 1 mL Braun PZN 00896456 EAN 4022495252074 Injection needle (pierce the filter) 21G34 4/5; 0,8 3 120 mm Braun PZN 04291448/EAN 4022495052414 Injection needle (injection of Heparin) 26G31; 0,45 3 25 mm Braun PZN 2050858 EAN 4022495001016 Metal multi-hook for glass infusion bottles Braun PZN 04800507/EAN 4022495260161 Infusion accessories Heidelberg extensions 75 cm Braun PZN 02133113/EAN 4022495004758 Braunostat® U Universal infusion stand Braun 8727902 Discofix®-3 three-way tap with connecting pipe, 50 cm, blue Braun PZN 07128507/EAN 4022495005007 Zeiss LSM 710 Confocal Inverted Microscope Zeiss RRID:SCR_018063 Leica STELLARIS 5 Microscope Leica RRID:SCR_024663 Pre-rinse solution Reagent Final concentration Amount Polyvinylpyrrolidone K30 a 2.5% 25 g Sodium chloride 0.9% 9 g Procaine hydrochloride a 0.5% 5 g Heparin natrium 25.000 I.E./5 mL a 10000 I.E./l 2 mL ddH 2 O N/A 1000 mL Total N/A 1000 mL Stored for up to 4 days at 2 ◦ C–8 ◦ C or freeze at − 20 ◦ C. a Harmful or toxic; handle with care. ..
Inverted Microscopy:Article Title: Preparation of cleared whole-mount urethra and urinary bladder from transcardially perfused mice for immunolabeling and analysis by CLSM.
Article Snippet: .. Dissecting scissors, medium FST 14184–09 Dissecting scissors, fine 14 cm straight FST 15020–15 Forceps, fine FST 11252–50 Forceps, coarse FST 11992–12 Clamp (artery clamp) FST 13018–14 TAIJI-IE Small Animal Anesthesia Machine RWD TAIJI-IE Infusion bottle (1000 mL, clear, Stopper Infusion 32 mm) or Infusion bottle, for example Braun’s isotonic sodium chloride solution 0.9% Packsys Braun 10000263 PZN:08646871/ EAN4030539036313 Glass-tubing cutter Merck Z150770-1EA Pravaz needle with LL mount, blunt version, size 1 diameter 0.9 mm at least 38 mm length or Laboratory pipetting needle with 90 ◦ blunt ends, gauge 20, L 4 in., 304 stainless steel hub Acufirm Merck 1400 LL CAD7950-12EA Scienceware® trays; L 3 W 3 H 30 cm 3 41 cm 3 7.6 cm Merck Z503797-1EA Stainless steel perforated sheet, square hole 10 mm - thickness 2.00 mm; L 3 W 31 cm 3 42 cm Elbemetal www.elbemetall.de Intrafix® SafeSet with LL mount 180 cm with 3 3 4.1 mm tubing Braun PZN 01900697/EAN 4022495869517 Syring 1 mL Braun PZN 00896456 EAN 4022495252074 Injection needle (pierce the filter) 21G34 4/5; 0,8 3 120 mm Braun PZN 04291448/EAN 4022495052414 Injection needle (injection of Heparin) 26G31; 0,45 3 25 mm Braun PZN 2050858 EAN 4022495001016 Metal multi-hook for glass infusion bottles Braun PZN 04800507/EAN 4022495260161 Infusion accessories Heidelberg extensions 75 cm Braun PZN 02133113/EAN 4022495004758 Braunostat® U Universal infusion stand Braun 8727902 Discofix®-3 three-way tap with connecting pipe, 50 cm, blue Braun PZN 07128507/EAN 4022495005007 Zeiss LSM 710 Confocal Inverted Microscope Zeiss RRID:SCR_018063 Leica STELLARIS 5 Microscope Leica RRID:SCR_024663 Pre-rinse solution Reagent Final concentration Amount Polyvinylpyrrolidone K30 a 2.5% 25 g Sodium chloride 0.9% 9 g Procaine hydrochloride a 0.5% 5 g Heparin natrium 25.000 I.E./5 mL a 10000 I.E./l 2 mL ddH 2 O N/A 1000 mL Total N/A 1000 mL Stored for up to 4 days at 2 ◦ C–8 ◦ C or freeze at − 20 ◦ C. a Harmful or toxic; handle with care. ..
Microscopy:Article Title: Preparation of cleared whole-mount urethra and urinary bladder from transcardially perfused mice for immunolabeling and analysis by CLSM.
Article Snippet: .. Dissecting scissors, medium FST 14184–09 Dissecting scissors, fine 14 cm straight FST 15020–15 Forceps, fine FST 11252–50 Forceps, coarse FST 11992–12 Clamp (artery clamp) FST 13018–14 TAIJI-IE Small Animal Anesthesia Machine RWD TAIJI-IE Infusion bottle (1000 mL, clear, Stopper Infusion 32 mm) or Infusion bottle, for example Braun’s isotonic sodium chloride solution 0.9% Packsys Braun 10000263 PZN:08646871/ EAN4030539036313 Glass-tubing cutter Merck Z150770-1EA Pravaz needle with LL mount, blunt version, size 1 diameter 0.9 mm at least 38 mm length or Laboratory pipetting needle with 90 ◦ blunt ends, gauge 20, L 4 in., 304 stainless steel hub Acufirm Merck 1400 LL CAD7950-12EA Scienceware® trays; L 3 W 3 H 30 cm 3 41 cm 3 7.6 cm Merck Z503797-1EA Stainless steel perforated sheet, square hole 10 mm - thickness 2.00 mm; L 3 W 31 cm 3 42 cm Elbemetal www.elbemetall.de Intrafix® SafeSet with LL mount 180 cm with 3 3 4.1 mm tubing Braun PZN 01900697/EAN 4022495869517 Syring 1 mL Braun PZN 00896456 EAN 4022495252074 Injection needle (pierce the filter) 21G34 4/5; 0,8 3 120 mm Braun PZN 04291448/EAN 4022495052414 Injection needle (injection of Heparin) 26G31; 0,45 3 25 mm Braun PZN 2050858 EAN 4022495001016 Metal multi-hook for glass infusion bottles Braun PZN 04800507/EAN 4022495260161 Infusion accessories Heidelberg extensions 75 cm Braun PZN 02133113/EAN 4022495004758 Braunostat® U Universal infusion stand Braun 8727902 Discofix®-3 three-way tap with connecting pipe, 50 cm, blue Braun PZN 07128507/EAN 4022495005007 Zeiss LSM 710 Confocal Inverted Microscope Zeiss RRID:SCR_018063 Leica STELLARIS 5 Microscope Leica RRID:SCR_024663 Pre-rinse solution Reagent Final concentration Amount Polyvinylpyrrolidone K30 a 2.5% 25 g Sodium chloride 0.9% 9 g Procaine hydrochloride a 0.5% 5 g Heparin natrium 25.000 I.E./5 mL a 10000 I.E./l 2 mL ddH 2 O N/A 1000 mL Total N/A 1000 mL Stored for up to 4 days at 2 ◦ C–8 ◦ C or freeze at − 20 ◦ C. a Harmful or toxic; handle with care. ..
Concentration Assay:Article Title: Preparation of cleared whole-mount urethra and urinary bladder from transcardially perfused mice for immunolabeling and analysis by CLSM.
Article Snippet: .. Dissecting scissors, medium FST 14184–09 Dissecting scissors, fine 14 cm straight FST 15020–15 Forceps, fine FST 11252–50 Forceps, coarse FST 11992–12 Clamp (artery clamp) FST 13018–14 TAIJI-IE Small Animal Anesthesia Machine RWD TAIJI-IE Infusion bottle (1000 mL, clear, Stopper Infusion 32 mm) or Infusion bottle, for example Braun’s isotonic sodium chloride solution 0.9% Packsys Braun 10000263 PZN:08646871/ EAN4030539036313 Glass-tubing cutter Merck Z150770-1EA Pravaz needle with LL mount, blunt version, size 1 diameter 0.9 mm at least 38 mm length or Laboratory pipetting needle with 90 ◦ blunt ends, gauge 20, L 4 in., 304 stainless steel hub Acufirm Merck 1400 LL CAD7950-12EA Scienceware® trays; L 3 W 3 H 30 cm 3 41 cm 3 7.6 cm Merck Z503797-1EA Stainless steel perforated sheet, square hole 10 mm - thickness 2.00 mm; L 3 W 31 cm 3 42 cm Elbemetal www.elbemetall.de Intrafix® SafeSet with LL mount 180 cm with 3 3 4.1 mm tubing Braun PZN 01900697/EAN 4022495869517 Syring 1 mL Braun PZN 00896456 EAN 4022495252074 Injection needle (pierce the filter) 21G34 4/5; 0,8 3 120 mm Braun PZN 04291448/EAN 4022495052414 Injection needle (injection of Heparin) 26G31; 0,45 3 25 mm Braun PZN 2050858 EAN 4022495001016 Metal multi-hook for glass infusion bottles Braun PZN 04800507/EAN 4022495260161 Infusion accessories Heidelberg extensions 75 cm Braun PZN 02133113/EAN 4022495004758 Braunostat® U Universal infusion stand Braun 8727902 Discofix®-3 three-way tap with connecting pipe, 50 cm, blue Braun PZN 07128507/EAN 4022495005007 Zeiss LSM 710 Confocal Inverted Microscope Zeiss RRID:SCR_018063 Leica STELLARIS 5 Microscope Leica RRID:SCR_024663 Pre-rinse solution Reagent Final concentration Amount Polyvinylpyrrolidone K30 a 2.5% 25 g Sodium chloride 0.9% 9 g Procaine hydrochloride a 0.5% 5 g Heparin natrium 25.000 I.E./5 mL a 10000 I.E./l 2 mL ddH 2 O N/A 1000 mL Total N/A 1000 mL Stored for up to 4 days at 2 ◦ C–8 ◦ C or freeze at − 20 ◦ C. a Harmful or toxic; handle with care. ..
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