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Clinical and Laboratory Standards Institute disk-diffusion susceptibility testing
Disk Diffusion Susceptibility Testing, supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/disk+diffusion+susceptibility+testing/disk+diffusion+test/pm40024542-60-6-17
Average 90 stars, based on 1 article reviews
disk-diffusion susceptibility testing - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Screening, Characterization, and Identification of Microbial Probiotic Extracts From Wastewater Treatments Plants Using 16S rDNA Sequencing
Article Snippet: .. The PCR confirmed isolates were also tested for antibiotic resistance using disk diffusion test as described by Clinical and Laboratory Standards Institute (CLSI) [12]. .. Nine commercial antibiotic discs (Table 2) that were sourced from Condalab in SA were used for the susceptibility test.

Diffusion-based Assay:

Article Title: Screening, Characterization, and Identification of Microbial Probiotic Extracts From Wastewater Treatments Plants Using 16S rDNA Sequencing
Article Snippet: .. The PCR confirmed isolates were also tested for antibiotic resistance using disk diffusion test as described by Clinical and Laboratory Standards Institute (CLSI) [12]. .. Nine commercial antibiotic discs (Table 2) that were sourced from Condalab in SA were used for the susceptibility test.

Article Title: Parabens alter the surface characteristics and antibiotic susceptibility of drinking water bacteria.
Article Snippet: .. The susceptibility to antibiotics was evaluated in the 5th and 10th weeks by the disk diffusion susceptibility methods according to the Clinical and Laboratory Standards Institute (CLSI, 2015). .. CEF at 30 μg; LEV at 5 μg; MINO at 30 μg and TMP-SMX at 1.25/23.75 μg were the selected antibiotics, according to the CLSI guidelines (CLSI, 2015) For that, bacteria were adjusted to 0.135 at 600 nm to match 0.5 MacFarland and spread on Mueller-Hinton agar – MHA (Merck, Germany) for all conditions.

Article Title: Rates of Multidrug-Resistant Gram-Negative Bacterial Infections in Hospitalized Non-Immunocompromised Pediatric Patients: A 9-Year Retrospective Study at a Lebanese Tertiary Medical Center
Article Snippet: Isolates of GNB recovered from clinical samples submitted to the Clinical Microbiology Laboratory, Department of Pathology and Laboratory Medicine, AUBMC, were identified using the standard biomedical methods or the matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) system (Bruker Daltonik, GmbH, Bremen, Germany) starting 2015. .. The antimicrobial susceptibility testing was performed using the disk diffusion test and interpreted according to the Clinical and Laboratory Standards Institute (CLSI) guidelines published each year. .. When requested by the medical team, the minimal inhibitory concentrations (MICs) of carbapenems, colistin and tigecycline were determined, using the E-test (PDM-Epsilometer, AB Biodisk, Solna, Sweden) in accordance with the manufacturer’s guidelines.

Article Title: Increased rate of multidrug-resistant gram-negative bacterial infections in hospitalized immunocompromised pediatric patients
Article Snippet: Isolates of GNB recovered from clinical samples submitted to the Clinical Microbiology Laboratory, Department of Pathology and Laboratory Medicine, AUBMC, were identified based on standard biochemical methods or using the matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) system (Bruker Daltonik, GmbH, Bremen, Germany) starting 2015. .. The antimicrobial susceptibility testing was performed using the disk diffusion test and interpreted according to the Clinical and Laboratory Standards Institute (CLSI) guidelines published for each year. .. The minimal inhibitory concentrations (MICs) of carbapenems, colistin and tigecycline were determined when requested by the medical team, using the E-test (PDM-Epsilometer, AB Biodisk, Solna, Sweden) according to the manufacturer’s guidelines.

Article Title: Gelatin film incorporated with pink pepper essential oil: physical properties and antimicrobial activity through direct contact and micro atmosphere
Article Snippet: Several studies have investigated using biodegradable materials as alternatives to plastic packaging.. Adding bioactive compounds, such as essential oils, to films enables the formation of active packaging.. This study aimed to develop active biodegradable gelatin films incorporating varying concentrations (0, 2, 4, and 6 %, v/w) of pink pepper essential oil (PPEO).

other:

Article Title: Multicenter evaluation of the eQUANT system for use with disk diffusion AST of gram-negative bacteria directly from positive blood cultures
Article Snippet: In addition, modifications of the disk diffusion (DD) testing method have been developed for rapid AST directly from positive blood cultures; such methods have subsequently been approved by the Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) ( , ).

Article Title: Multicenter evaluation of the eQUANT system for use with disk diffusion AST of gram-negative bacteria directly from positive blood cultures.
Article Snippet: In addition, modifications of the disk diffusion (DD) testing method have been developed for rapid AST directly from positive blood cultures; such methods have subsequently been approved by the Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) (12, 13).

Article Title: The effect of quaternary ammonium compounds (QACs) on quorum sensing and resistance of P. aeruginosa in clinical settings.
Article Snippet: Pseudomonas aeruginosa, a formidable opportunistic pathogen, is notorious for its ability to form biofilms and produce virulence factors that favor chronic infections, especially in cystic fibrosis patients.. The misuse of disinfectants, combined with environmental leakage and biodegradation, has led to widespread exposure of microorganisms to sub-lethal concentrations of disinfectants, particularly quaternary ammonium compounds (QACs).. This study investigates the interaction between QACs, specifically ethylbenzalkyl dimethyl ammonium chloride (EBAC), and the quorum sensing (QS) mechanisms governing P. aeruginosa behavior.



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<t> Antimicrobial susceptibility </t> profile for Staphylococcus aureus .
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Antimicrobial <t> susceptibility </t> testing of EAV_NVB490, EAV_NNN508, EAV_VTKC53 and EAV_NVH72 using the VITEK2 Compact system (MIC, in mg/L; left) and <t> DDT </t> (inhibition zone diameter, in mm; right).
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 Antimicrobial susceptibility  profile for Staphylococcus aureus .

Journal: PLOS Global Public Health

Article Title: Prevalence, resistance profiles and factors associated with skin and soft-tissue infections at Jinja regional referral hospital: A retrospective study

doi: 10.1371/journal.pgph.0003582

Figure Lengend Snippet: Antimicrobial susceptibility profile for Staphylococcus aureus .

Article Snippet: Antimicrobial susceptibility testing(AST) was done using the Kirby-Bauer disk-diffusion method and interpreted according to the Clinical and Laboratory Standards Institute (CLSI) guidelines [ – ].

Techniques: Concentration Assay

 Antimicrobial susceptibility  profile for Enterobacterales .

Journal: PLOS Global Public Health

Article Title: Prevalence, resistance profiles and factors associated with skin and soft-tissue infections at Jinja regional referral hospital: A retrospective study

doi: 10.1371/journal.pgph.0003582

Figure Lengend Snippet: Antimicrobial susceptibility profile for Enterobacterales .

Article Snippet: Antimicrobial susceptibility testing(AST) was done using the Kirby-Bauer disk-diffusion method and interpreted according to the Clinical and Laboratory Standards Institute (CLSI) guidelines [ – ].

Techniques: Concentration Assay

Antimicrobial  susceptibility  testing of EAV_NVB490, EAV_NNN508, EAV_VTKC53 and EAV_NVH72 using the VITEK2 Compact system (MIC, in mg/L; left) and  DDT  (inhibition zone diameter, in mm; right).

Journal: Scientific Reports

Article Title: Whole-genome sequence and resistance determinants of four Elizabethkingia anophelis clinical isolates collected in Hanoi, Vietnam

doi: 10.1038/s41598-024-57564-3

Figure Lengend Snippet: Antimicrobial susceptibility testing of EAV_NVB490, EAV_NNN508, EAV_VTKC53 and EAV_NVH72 using the VITEK2 Compact system (MIC, in mg/L; left) and DDT (inhibition zone diameter, in mm; right).

Article Snippet: Upon arrival at USTH, the Kirby-Bauer Disk Diffusion Susceptibility Test (DDT) was used with 43 antibiotics (Thermo Scientific, UK): ampicillin (10 μg), penicillin G (1UI), oxacillin (5 μg), temocillin (30 μg), ticarcillin (75 μg), amoxicillin/clavulanic acid (20/10 μg), ticarcillin/clavulanic acid (75/10 μg), piperacillin/tazobactam (100/10 μg), cefotaxime (30 μg), cefoxitin (30 μg), cefepime (30 μg), ceftazidime (30 μg), cefpodoxime (10 μg), cephalexin (30 μg), imipenem (10 μg), meropenem (10 μg), ertapenem (10 μg), aztreonam (30 μg), gentamicin (10 μg), tobramycin (10 μg), amikacin (30 μg), kanamycin (30 μg), netilmicin (10 μg), ciprofloxacin (5 μg), norfloxacin (10 μg), levofloxacin (5 μg), ofloxacin (5 μg), nalidixic acid (30 μg), trimethoprim/sulfamethoxazole (1.25/23.75 μg), colistin (10 μg), tetracycline (30 μg), minocycline (30 μg), erythromycin (15 μg), chloramphenicol (30 μg), rifampicin (5 μg), vancomycin (5 μg), teicoplanin (30 μg), fusidic acid (10 μg), fosfomycin (200 μg), linezolid (10 μg), pristinamycin (15 μg), clindamycin (2 μg) and lincomycin (15 μg) following the method described in the CLSI guidelines (CLSI M02, 13th edition, 2018).

Techniques: Inhibition