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pcr-direct sequence method  (Solid Phase Inc)

 
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    Solid Phase Inc pcr-direct sequence method
    Pcr Direct Sequence Method, supplied by Solid Phase Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/direct+sequencing+pcr+method/pcr+direct+sequence+method/pm09589656-74-8-8
    Average 90 stars, based on 1 article reviews
    pcr-direct sequence method - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: A case of Albright's hereditary osteodystrophy-like syndrome complicated by several endocrinopathies: normal Gs alpha gene and chromosome 2q37.
    Article Snippet: The Gsa mutations were identified both by a solidphase PCR-direct sequence method and by the sequencing of the amplified DNA fragments subcloned into the plasmid.

    Sequencing:

    Article Title: A case of Albright's hereditary osteodystrophy-like syndrome complicated by several endocrinopathies: normal Gs alpha gene and chromosome 2q37.
    Article Snippet: The Gsa mutations were identified both by a solidphase PCR-direct sequence method and by the sequencing of the amplified DNA fragments subcloned into the plasmid.

    Amplification:

    Article Title: A case of Albright's hereditary osteodystrophy-like syndrome complicated by several endocrinopathies: normal Gs alpha gene and chromosome 2q37.
    Article Snippet: The Gsa mutations were identified both by a solidphase PCR-direct sequence method and by the sequencing of the amplified DNA fragments subcloned into the plasmid.

    Plasmid Preparation:

    Article Title: A case of Albright's hereditary osteodystrophy-like syndrome complicated by several endocrinopathies: normal Gs alpha gene and chromosome 2q37.
    Article Snippet: The Gsa mutations were identified both by a solidphase PCR-direct sequence method and by the sequencing of the amplified DNA fragments subcloned into the plasmid.



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    Image Search Results


    Comparative analysis of aligned sequencing reads on the human BCL2L12 gene, derived from the presented 5′ RACE-seq approach and publicly available Direct RNA sequencing datasets. A Visualization of the aligned sequencing reads derived from the presented 5′ RACE-seq approach. B Visualization of the aligned sequencing reads derived from publicly available Direct RNA sequencing datasets deposited on the Sequence Read Archive database (SRA). C Indicative raw nanopore sequencing reads from the generated FASTQ file of the presented 5′ RACE-seq approach, highlighting the incorporation of the drTSO specifically to the 5′ end of each target mRNA transcript

    Journal: BMC Genomics

    Article Title: A versatile 5′ RACE-Seq methodology for the accurate identification of the 5′ termini of mRNAs

    doi: 10.1186/s12864-022-08386-y

    Figure Lengend Snippet: Comparative analysis of aligned sequencing reads on the human BCL2L12 gene, derived from the presented 5′ RACE-seq approach and publicly available Direct RNA sequencing datasets. A Visualization of the aligned sequencing reads derived from the presented 5′ RACE-seq approach. B Visualization of the aligned sequencing reads derived from publicly available Direct RNA sequencing datasets deposited on the Sequence Read Archive database (SRA). C Indicative raw nanopore sequencing reads from the generated FASTQ file of the presented 5′ RACE-seq approach, highlighting the incorporation of the drTSO specifically to the 5′ end of each target mRNA transcript

    Article Snippet: Our results led to the accurate identification of the 5′ UTRs of the BCL2L12 gene, being in accordance with the 5′ ends provided by both direct RNA and PCR-cDNA sequencing method from Oxford Nanopore Technologies.

    Techniques: Sequencing, Derivative Assay, RNA Sequencing, Nanopore Sequencing, Generated

    Template-switching oligonucleotides (TSOs) and 5′ RACE primers used in the present work

    Journal: BMC Genomics

    Article Title: A versatile 5′ RACE-Seq methodology for the accurate identification of the 5′ termini of mRNAs

    doi: 10.1186/s12864-022-08386-y

    Figure Lengend Snippet: Template-switching oligonucleotides (TSOs) and 5′ RACE primers used in the present work

    Article Snippet: Our results led to the accurate identification of the 5′ UTRs of the BCL2L12 gene, being in accordance with the 5′ ends provided by both direct RNA and PCR-cDNA sequencing method from Oxford Nanopore Technologies.

    Techniques: Sequencing, Modification