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vybrant dir cell-labeling solution d-12731  (Thermo Fisher)


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    Thermo Fisher vybrant dir cell-labeling solution d-12731
    Vybrant Dir Cell Labeling Solution D 12731, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dir+solution/dir+d12731/pm37798868-51-12-17
    Average 90 stars, based on 1 article reviews
    vybrant dir cell-labeling solution d-12731 - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Cell Culture:

    Article Title: In vivo bioengineered tooth formation using decellularized tooth bud extracellular matrix scaffolds.
    Article Snippet: .. Constructing bioengineered tooth buds Cultured cells were labeled as follows. pDEs were labeled with DiO (V22886, Thermo Fisher Scientific, Waltham, MA), hDPCs were labeled with DiD (V22887, Thermo Fisher Scientific), and HUVECs were labeled with DiR (D12731, Thermo Fisher Scientific). ..

    Article Title: In vivo bioengineered tooth formation using decellularized tooth bud extracellular matrix scaffolds
    Article Snippet: Human dental pulp cells (hDPCs) and porcine tooth bud derived dental epithelial cells (pDEs) were harvested and characterized as previously published by us., The Human umbilical vein endothelial cells (HUVEC) cell line was purchased from ATCC (PSC100010, Manassas, VA), expanded in vascular basal media (PCS100030, ATCC) with VEGF growth kit (PCS100041, ATCC) in 5% CO 2 at 37 °C, and cryopreserved at passage 3. .. Cultured cells were labeled as follows. pDEs were labeled with DiO (V22886, Thermo Fisher Scientific, Waltham, MA), hDPCs were labeled with DiD (V22887, Thermo Fisher Scientific), and HUVECs were labeled with DiR (D12731, Thermo Fisher Scientific). ..

    Labeling:

    Article Title: In vivo bioengineered tooth formation using decellularized tooth bud extracellular matrix scaffolds.
    Article Snippet: .. Constructing bioengineered tooth buds Cultured cells were labeled as follows. pDEs were labeled with DiO (V22886, Thermo Fisher Scientific, Waltham, MA), hDPCs were labeled with DiD (V22887, Thermo Fisher Scientific), and HUVECs were labeled with DiR (D12731, Thermo Fisher Scientific). ..

    Article Title: In vivo bioengineered tooth formation using decellularized tooth bud extracellular matrix scaffolds
    Article Snippet: Human dental pulp cells (hDPCs) and porcine tooth bud derived dental epithelial cells (pDEs) were harvested and characterized as previously published by us., The Human umbilical vein endothelial cells (HUVEC) cell line was purchased from ATCC (PSC100010, Manassas, VA), expanded in vascular basal media (PCS100030, ATCC) with VEGF growth kit (PCS100041, ATCC) in 5% CO 2 at 37 °C, and cryopreserved at passage 3. .. Cultured cells were labeled as follows. pDEs were labeled with DiO (V22886, Thermo Fisher Scientific, Waltham, MA), hDPCs were labeled with DiD (V22887, Thermo Fisher Scientific), and HUVECs were labeled with DiR (D12731, Thermo Fisher Scientific). ..

    other:

    Article Title: CRISPR/Cas9-mediated SHP-1-knockout T cells combined with simvastatin enhances anti-tumor activity in humanized-PDX HCC model
    Article Snippet: SHP-1-KO T cells and WT T cells were incubated with 2.5 μg/mL DiR (#D12731, ThermoFisher) staining solution for 5 min, washed twice with homemade termination solution (1640 medium +10% FBS), and resuspended in normal saline and injected into mice.

    Article Title: Harnessing Microneedles for Delivery and Preservation of Natural Killer Cell-Derived Extracellular Vesicles.
    Article Snippet: Natural killer cell-derived extracellular vesicles (NKEVs) have demonstrated anti-inflammatory properties similar to those of their parent cells.. EVs have been commonly delivered via intravenous (IV) administration, which can be invasive and is not ideal for chronic treatment.. Another limitation of nanotherapy is its storage requirements, as EVs are commonly stored at −80 °C to preserve EV cargo and stability.

    Article Title: Decellularized tissue matrices hydrogels functionalized with extracellular vesicles promote macrophage reprogramming and neural stem cell differentiation for spinal cord injury repair.
    Article Snippet: DiR (D12731, Thermo Fisher Scientific, USA) was added to the EV solution at a concentration of 1:400 and incubated for 30 min, followed by ultracentrifugation at 100,000 g for 90 min to remove excess dye.

    Article Title: CRISPR/Cas9-Mediated SHP-1-Knockout T cells Combined with Simvastatin Enhances Anti-Tumor Activity in Humanized-PDX HCC Model
    Article Snippet: SHP-1-KO T cells and WT T cells were incubated with 2.5 μg/mL DiR (#D12731, ThermoFisher) 33 staining solution for 5 min, washed twice with homemade termination solution (1640 medium 34 +10% FBS), and resuspended in normal saline and injected into mice.

    Article Title: Harnessing bone marrow mesenchymal stem cell-derived extracellular vesicles and biomimetic peptide WKYMVm in self-healing hydrogel for enhanced bone repair in femoral defects
    Article Snippet: DiR dye (D12731, Thermo Fisher Scientific, USA) was added to the EV solution at a concentration of 1:400 and incubated for 30 min to obtain DiR-labeled EVs.

    In Vivo:

    Article Title: Mitochondrial energy reprogramming via dECM/GP@EVs@Gpnmb to prevent post-myocardial infarction heart failure
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Fluorescence:

    Article Title: Mitochondrial energy reprogramming via dECM/GP@EVs@Gpnmb to prevent post-myocardial infarction heart failure
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Imaging:

    Article Title: Mitochondrial energy reprogramming via dECM/GP@EVs@Gpnmb to prevent post-myocardial infarction heart failure
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.



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    Becton Dickinson bnn6/dir mixture solution
    The molecularly engineered nano-penetrator was self-assembled by <t>BNN6-DiR</t> fuel pair without the assistance of any carrier materials. After PEGylation and fibrin-homing decoration, such a self-navigated, self‐fueled, and self-propelled nano-penetrator displayed satisfactory therapeutic outcomes in artery/vein thrombosis and acute ischemic stroke animal models.
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    Image Search Results


    The molecularly engineered nano-penetrator was self-assembled by BNN6-DiR fuel pair without the assistance of any carrier materials. After PEGylation and fibrin-homing decoration, such a self-navigated, self‐fueled, and self-propelled nano-penetrator displayed satisfactory therapeutic outcomes in artery/vein thrombosis and acute ischemic stroke animal models.

    Journal: Nature Communications

    Article Title: Molecularly self‐fueled nano-penetrator for nonpharmaceutical treatment of thrombosis and ischemic stroke

    doi: 10.1038/s41467-023-35895-5

    Figure Lengend Snippet: The molecularly engineered nano-penetrator was self-assembled by BNN6-DiR fuel pair without the assistance of any carrier materials. After PEGylation and fibrin-homing decoration, such a self-navigated, self‐fueled, and self-propelled nano-penetrator displayed satisfactory therapeutic outcomes in artery/vein thrombosis and acute ischemic stroke animal models.

    Article Snippet: As shown in Fig. , the temperature of DiR Sol, BNN6/DiR mixture solution (BD Sol), B-BD NAs, N-BD NAs, and T-BD NAs continued to rise to >50 °C within 10 min under laser irradiation, whereas the temperature of PBS (pH 7.4) did not dramatically change under the same conditions.

    Techniques:

    a Chemical structure of DiR, BNN6 (NO donor), and BPA (BNN6 precursor without NO production ability). b Co-assembly process of the binary NAs. c NO generation from B-BD NAs at various molar ratios of DiR and BNN6 from 10:1 to 1:10. Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. d – f Particle sizes distribution profiles and TEM images of B-BD NAs, T-BD NAs, and N-BD NAs, respectively, (Scale bar = 100 nm). Experiment was repeated three times independently with similar results. g Colloidal stability of B-BD NAs, T-BD NAs, and N-BD NAs incubated in PBS (pH 7.4) within 8 h at 37 ° C. Data are presented as mean ± SD ( n = 3, independent experiments). h In vitro thrombus-targeting fluorescence intensity in artificial blood clots treated with PBS (pH 7.4), DiR Sol, B-BD NAs, N-BD NAs, and T-BD NAs, respectively. Data are presented as mean ± SD ( n = 3, independent experiments). One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. The n.s. represent no significance. i Molecular docking simulation results of BNN6 and DiR. j Particle size changes of B-BD NAs and T-BD NAs in the presence of urea (50 mM), NaCl (50 mM), and SDS (50 mM), respectively ( n = 3, independent experiments). k UV absorption spectra of DiR Sol, BNN6 Sol, B-BD NAs, and T-BD NAs. l Fluorescence spectra of DiR Sol, B-BD NAs, and T-BD NAs.

    Journal: Nature Communications

    Article Title: Molecularly self‐fueled nano-penetrator for nonpharmaceutical treatment of thrombosis and ischemic stroke

    doi: 10.1038/s41467-023-35895-5

    Figure Lengend Snippet: a Chemical structure of DiR, BNN6 (NO donor), and BPA (BNN6 precursor without NO production ability). b Co-assembly process of the binary NAs. c NO generation from B-BD NAs at various molar ratios of DiR and BNN6 from 10:1 to 1:10. Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. d – f Particle sizes distribution profiles and TEM images of B-BD NAs, T-BD NAs, and N-BD NAs, respectively, (Scale bar = 100 nm). Experiment was repeated three times independently with similar results. g Colloidal stability of B-BD NAs, T-BD NAs, and N-BD NAs incubated in PBS (pH 7.4) within 8 h at 37 ° C. Data are presented as mean ± SD ( n = 3, independent experiments). h In vitro thrombus-targeting fluorescence intensity in artificial blood clots treated with PBS (pH 7.4), DiR Sol, B-BD NAs, N-BD NAs, and T-BD NAs, respectively. Data are presented as mean ± SD ( n = 3, independent experiments). One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. The n.s. represent no significance. i Molecular docking simulation results of BNN6 and DiR. j Particle size changes of B-BD NAs and T-BD NAs in the presence of urea (50 mM), NaCl (50 mM), and SDS (50 mM), respectively ( n = 3, independent experiments). k UV absorption spectra of DiR Sol, BNN6 Sol, B-BD NAs, and T-BD NAs. l Fluorescence spectra of DiR Sol, B-BD NAs, and T-BD NAs.

    Article Snippet: As shown in Fig. , the temperature of DiR Sol, BNN6/DiR mixture solution (BD Sol), B-BD NAs, N-BD NAs, and T-BD NAs continued to rise to >50 °C within 10 min under laser irradiation, whereas the temperature of PBS (pH 7.4) did not dramatically change under the same conditions.

    Techniques: Incubation, In Vitro, Fluorescence

    In vitro photothermal heating a image and b curves under laser irradiation (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3 independent experiments). Source data are provided as a Source Data file. c In vitro photothermal conversion efficiency of T-BD NAs. d NO release curves under laser irradiation (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. e Chemical mechanisms of NO generation from BNN6: intramolecular rearrangement and homogenous cleavage of N-NO bonds. f NO release curves of BNN6 Sol and T-BD NAs under heating (50 °C) or laser irradiation (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. g Off-on NO generation variations by the switch of laser irradiation (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. h Schematic representation of NO generation and autonomous motion features of carrier-free NAs and carrier-based NPs. i Laser-triggered NO release from T-BD NAs and PLGA-BD NAs at the same dose of DiR/BNN6 (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. j The movement trajectories of T-BD NAs and PLGA-BD NAs incubated in PBS (pH 7.4) at the same dose of DiR/BNN6 under laser irradiations (0 W/cm 2 and 2 W/cm 2 , 60 s), scale bar = 1 μm. k The motion speed of T-BD NAs and PLGA-BD NPs incubated in PBS (pH 7.4) at the same dose of DiR/BNN6 under laser irradiations (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. The n.s. represent no significance. Yellow lightning represents laser irradiation. L and H represent laser and heating, respectively.

    Journal: Nature Communications

    Article Title: Molecularly self‐fueled nano-penetrator for nonpharmaceutical treatment of thrombosis and ischemic stroke

    doi: 10.1038/s41467-023-35895-5

    Figure Lengend Snippet: In vitro photothermal heating a image and b curves under laser irradiation (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3 independent experiments). Source data are provided as a Source Data file. c In vitro photothermal conversion efficiency of T-BD NAs. d NO release curves under laser irradiation (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. e Chemical mechanisms of NO generation from BNN6: intramolecular rearrangement and homogenous cleavage of N-NO bonds. f NO release curves of BNN6 Sol and T-BD NAs under heating (50 °C) or laser irradiation (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. g Off-on NO generation variations by the switch of laser irradiation (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. h Schematic representation of NO generation and autonomous motion features of carrier-free NAs and carrier-based NPs. i Laser-triggered NO release from T-BD NAs and PLGA-BD NAs at the same dose of DiR/BNN6 (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. j The movement trajectories of T-BD NAs and PLGA-BD NAs incubated in PBS (pH 7.4) at the same dose of DiR/BNN6 under laser irradiations (0 W/cm 2 and 2 W/cm 2 , 60 s), scale bar = 1 μm. k The motion speed of T-BD NAs and PLGA-BD NPs incubated in PBS (pH 7.4) at the same dose of DiR/BNN6 under laser irradiations (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. The n.s. represent no significance. Yellow lightning represents laser irradiation. L and H represent laser and heating, respectively.

    Article Snippet: As shown in Fig. , the temperature of DiR Sol, BNN6/DiR mixture solution (BD Sol), B-BD NAs, N-BD NAs, and T-BD NAs continued to rise to >50 °C within 10 min under laser irradiation, whereas the temperature of PBS (pH 7.4) did not dramatically change under the same conditions.

    Techniques: In Vitro, Irradiation, Incubation

    a Schematic representation of photothermal/mechanical synergistic thrombolysis and clot-deep penetration. b Photos of blood clots after different treatments with/without the 808 nm NIR laser irradiation: (2 W/cm 2 , 20 min). c Fibrin and hemoglobin levels in the blood clot supernatants after different treatments with/without the 808 nm NIR laser irradiation. Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. The n.s. represent no significance. d Images of blood clots before and after different treatments with 808 nm NIR laser irradiation (2 W/cm 2 , 20 min), scale bar = 2 mm. e H&E staining results of d under 808 nm NIR laser irradiation (2 W/cm 2 , 20 min), scale bar = 100 μm. f Thrombolytic rates of d under 808 nm NIR laser irradiation (2 W/cm 2 , 20 min). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. The n.s. represent no significance. g CLSM images of small red thrombi incubated with free C-6, C6/B-BD NAs, C6/N-BD NAs, or C6/T-BD NAs under 808 nm laser irradiation (2 W/cm 2 , 15 min), scale bar = 100 μm. Experiment was repeated three times independently with similar results. h Quantitative results of the depth of clot penetration. (I: PBS, II: BNN6 Sol, III: DiR Sol, IV: BD Sol, V: B-BD NAs, VI: T-BD NAs, VII: N-BD NAs). The lightning bolt represents laser irradiation.

    Journal: Nature Communications

    Article Title: Molecularly self‐fueled nano-penetrator for nonpharmaceutical treatment of thrombosis and ischemic stroke

    doi: 10.1038/s41467-023-35895-5

    Figure Lengend Snippet: a Schematic representation of photothermal/mechanical synergistic thrombolysis and clot-deep penetration. b Photos of blood clots after different treatments with/without the 808 nm NIR laser irradiation: (2 W/cm 2 , 20 min). c Fibrin and hemoglobin levels in the blood clot supernatants after different treatments with/without the 808 nm NIR laser irradiation. Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. The n.s. represent no significance. d Images of blood clots before and after different treatments with 808 nm NIR laser irradiation (2 W/cm 2 , 20 min), scale bar = 2 mm. e H&E staining results of d under 808 nm NIR laser irradiation (2 W/cm 2 , 20 min), scale bar = 100 μm. f Thrombolytic rates of d under 808 nm NIR laser irradiation (2 W/cm 2 , 20 min). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. The n.s. represent no significance. g CLSM images of small red thrombi incubated with free C-6, C6/B-BD NAs, C6/N-BD NAs, or C6/T-BD NAs under 808 nm laser irradiation (2 W/cm 2 , 15 min), scale bar = 100 μm. Experiment was repeated three times independently with similar results. h Quantitative results of the depth of clot penetration. (I: PBS, II: BNN6 Sol, III: DiR Sol, IV: BD Sol, V: B-BD NAs, VI: T-BD NAs, VII: N-BD NAs). The lightning bolt represents laser irradiation.

    Article Snippet: As shown in Fig. , the temperature of DiR Sol, BNN6/DiR mixture solution (BD Sol), B-BD NAs, N-BD NAs, and T-BD NAs continued to rise to >50 °C within 10 min under laser irradiation, whereas the temperature of PBS (pH 7.4) did not dramatically change under the same conditions.

    Techniques: Irradiation, Staining, Incubation