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dir celllabeling solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dir celllabeling solution
    Dir Celllabeling Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dir+solution/pm36008710-249-8-12
    Average 86 stars, based on 1 article reviews
    dir celllabeling solution - by Bioz Stars, 2026-09
    86/100 stars

    Images

    Related Articles

    Labeling:

    Article Title: PEGylated β-Cell-Targeting Exosomes from Mesenchymal Stem Cells Improve β Cell Function and Quantity by Suppressing NRF2-Mediated Ferroptosis
    Article Snippet: .. MSC-EXOs and Apt-EXOs were labeled with DIO cell-labeling solution (Invitrogen, V22886, USA) for in vitro studies and with infrared fluorescent DIR Lipophilic dye (Invitrogen, D12731, USA) for in vivo studies. ..

    Article Title: PEGylated β-Cell-Targeting Exosomes from Mesenchymal Stem Cells Improve β Cell Function and Quantity by Suppressing NRF2-Mediated Ferroptosis
    Article Snippet: .. Cellular Internalization of Exosomes MSC-EXOs and Apt-EXOs were labeled with DIO cell-labeling solution (Invitrogen, V22886, USA) for in vitro studies and with infrared fluorescent DIR Lipophilic dye (Invitrogen, D12731, USA) for in vivo studies. ..

    In Vitro:

    Article Title: PEGylated β-Cell-Targeting Exosomes from Mesenchymal Stem Cells Improve β Cell Function and Quantity by Suppressing NRF2-Mediated Ferroptosis
    Article Snippet: .. MSC-EXOs and Apt-EXOs were labeled with DIO cell-labeling solution (Invitrogen, V22886, USA) for in vitro studies and with infrared fluorescent DIR Lipophilic dye (Invitrogen, D12731, USA) for in vivo studies. ..

    Article Title: Systemic Delivery of a STING Agonist-Loaded Positively Charged Liposome Selectively Targets Tumor Immune Microenvironment and Suppresses Tumor Angiogenesis.
    Article Snippet: Although stimulator of interferon genes (STING) agonists has shown great promise in preclinical studies, the clinical development of STING agonist therapy is challenged by its limited systemic delivery.. Here, positively charged fusogenic liposomes loaded with a STING agonist (PoSTING) are designed for systemic delivery and to preferentially target the tumor microenvironment.. When PoSTING is administered intravenously, it selectively targets not only tumor cells but also immune and tumor endothelial cells (ECs).

    Article Title: PEGylated β-Cell-Targeting Exosomes from Mesenchymal Stem Cells Improve β Cell Function and Quantity by Suppressing NRF2-Mediated Ferroptosis
    Article Snippet: .. Cellular Internalization of Exosomes MSC-EXOs and Apt-EXOs were labeled with DIO cell-labeling solution (Invitrogen, V22886, USA) for in vitro studies and with infrared fluorescent DIR Lipophilic dye (Invitrogen, D12731, USA) for in vivo studies. ..

    In Vivo:

    Article Title: PEGylated β-Cell-Targeting Exosomes from Mesenchymal Stem Cells Improve β Cell Function and Quantity by Suppressing NRF2-Mediated Ferroptosis
    Article Snippet: .. MSC-EXOs and Apt-EXOs were labeled with DIO cell-labeling solution (Invitrogen, V22886, USA) for in vitro studies and with infrared fluorescent DIR Lipophilic dye (Invitrogen, D12731, USA) for in vivo studies. ..

    Article Title: Systemic Delivery of a STING Agonist-Loaded Positively Charged Liposome Selectively Targets Tumor Immune Microenvironment and Suppresses Tumor Angiogenesis.
    Article Snippet: Although stimulator of interferon genes (STING) agonists has shown great promise in preclinical studies, the clinical development of STING agonist therapy is challenged by its limited systemic delivery.. Here, positively charged fusogenic liposomes loaded with a STING agonist (PoSTING) are designed for systemic delivery and to preferentially target the tumor microenvironment.. When PoSTING is administered intravenously, it selectively targets not only tumor cells but also immune and tumor endothelial cells (ECs).

    Article Title: PEGylated β-Cell-Targeting Exosomes from Mesenchymal Stem Cells Improve β Cell Function and Quantity by Suppressing NRF2-Mediated Ferroptosis
    Article Snippet: .. Cellular Internalization of Exosomes MSC-EXOs and Apt-EXOs were labeled with DIO cell-labeling solution (Invitrogen, V22886, USA) for in vitro studies and with infrared fluorescent DIR Lipophilic dye (Invitrogen, D12731, USA) for in vivo studies. ..

    Liposomes:

    Article Title: Systemic Delivery of a STING Agonist-Loaded Positively Charged Liposome Selectively Targets Tumor Immune Microenvironment and Suppresses Tumor Angiogenesis.
    Article Snippet: Although stimulator of interferon genes (STING) agonists has shown great promise in preclinical studies, the clinical development of STING agonist therapy is challenged by its limited systemic delivery.. Here, positively charged fusogenic liposomes loaded with a STING agonist (PoSTING) are designed for systemic delivery and to preferentially target the tumor microenvironment.. When PoSTING is administered intravenously, it selectively targets not only tumor cells but also immune and tumor endothelial cells (ECs).

    Cell Culture:

    Article Title: Methods for generating therapeutic delivery platforms
    Article Snippet: Once the cell cultures reached ̃70% confluency, the media was replaced with fresh media containing 10% exosome-depleted FBS (Thermo Fisher, A2720803). .. The cells were cultured for an additional 72 h before the conditioned media were collected. sEVs were isolated using Nanopoms approach. sEVs were incubated with 1 mM fluorescent lipophilic tracer DiR (1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide) (Invitrogen, D12731) at room temperature (RT) for 15 minutes. ..

    Isolation:

    Article Title: Methods for generating therapeutic delivery platforms
    Article Snippet: Once the cell cultures reached ̃70% confluency, the media was replaced with fresh media containing 10% exosome-depleted FBS (Thermo Fisher, A2720803). .. The cells were cultured for an additional 72 h before the conditioned media were collected. sEVs were isolated using Nanopoms approach. sEVs were incubated with 1 mM fluorescent lipophilic tracer DiR (1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide) (Invitrogen, D12731) at room temperature (RT) for 15 minutes. ..

    Incubation:

    Article Title: Methods for generating therapeutic delivery platforms
    Article Snippet: Once the cell cultures reached ̃70% confluency, the media was replaced with fresh media containing 10% exosome-depleted FBS (Thermo Fisher, A2720803). .. The cells were cultured for an additional 72 h before the conditioned media were collected. sEVs were isolated using Nanopoms approach. sEVs were incubated with 1 mM fluorescent lipophilic tracer DiR (1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide) (Invitrogen, D12731) at room temperature (RT) for 15 minutes. ..

    Article Title: Anticancer effect of hUC-MSC-derived exosome-mediated delivery of PMO-miR-146b-5p in colorectal cancer.
    Article Snippet: .. Purified exosomes (5.0 × 109 particles/mL) were incubated with 1 μM fluorescent lipophilic tracer DiR (D12731, Invitrogen, USA) for 5 min at 37 °C prior to 4 °C for another 15 min. Unbound dye was removed by centrifugation at 100,000 × g overnight by means of an SW41 Ti rotor (Backman), and then, the pellet was washed twice with precooled PBS. .. The obtained DiR-Exos were resuspended in precooled PBS, and the particle concentration was measured by nanoFCM.

    other:

    Article Title: IL-11-Engineered Macrophage Membrane-Coated Reactive Oxygen Species-Responsive Nanoparticles for Targeted Delivery of Doxorubicin to Osteosarcoma.
    Article Snippet: Osteosarcoma (OS) is a lethal malignant orthotopic bone tumor that primarily affects children and adolescents.. Biomimetic nanocarriers have attracted wide attention as a new strategy for delivering chemotherapy agents to the OS.. However, challenges such as rapid clearance and limited targeting hinder the effectiveness of OS chemotherapy.

    Article Title: Histone deacetylase inhibition enhances extracellular vesicles from muscle to promote osteogenesis via miR-873-3p
    Article Snippet: To track EV distribution, 100 μg of DiR-labeled EVs (D12731, Invitrogen) were injected into the tail vein of mice in a 200 μL PBS solution.

    Purification:

    Article Title: Anticancer effect of hUC-MSC-derived exosome-mediated delivery of PMO-miR-146b-5p in colorectal cancer.
    Article Snippet: .. Purified exosomes (5.0 × 109 particles/mL) were incubated with 1 μM fluorescent lipophilic tracer DiR (D12731, Invitrogen, USA) for 5 min at 37 °C prior to 4 °C for another 15 min. Unbound dye was removed by centrifugation at 100,000 × g overnight by means of an SW41 Ti rotor (Backman), and then, the pellet was washed twice with precooled PBS. .. The obtained DiR-Exos were resuspended in precooled PBS, and the particle concentration was measured by nanoFCM.

    Centrifugation:

    Article Title: Anticancer effect of hUC-MSC-derived exosome-mediated delivery of PMO-miR-146b-5p in colorectal cancer.
    Article Snippet: .. Purified exosomes (5.0 × 109 particles/mL) were incubated with 1 μM fluorescent lipophilic tracer DiR (D12731, Invitrogen, USA) for 5 min at 37 °C prior to 4 °C for another 15 min. Unbound dye was removed by centrifugation at 100,000 × g overnight by means of an SW41 Ti rotor (Backman), and then, the pellet was washed twice with precooled PBS. .. The obtained DiR-Exos were resuspended in precooled PBS, and the particle concentration was measured by nanoFCM.



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    Image Search Results


    The molecularly engineered nano-penetrator was self-assembled by BNN6-DiR fuel pair without the assistance of any carrier materials. After PEGylation and fibrin-homing decoration, such a self-navigated, self‐fueled, and self-propelled nano-penetrator displayed satisfactory therapeutic outcomes in artery/vein thrombosis and acute ischemic stroke animal models.

    Journal: Nature Communications

    Article Title: Molecularly self‐fueled nano-penetrator for nonpharmaceutical treatment of thrombosis and ischemic stroke

    doi: 10.1038/s41467-023-35895-5

    Figure Lengend Snippet: The molecularly engineered nano-penetrator was self-assembled by BNN6-DiR fuel pair without the assistance of any carrier materials. After PEGylation and fibrin-homing decoration, such a self-navigated, self‐fueled, and self-propelled nano-penetrator displayed satisfactory therapeutic outcomes in artery/vein thrombosis and acute ischemic stroke animal models.

    Article Snippet: As shown in Fig. , the temperature of DiR Sol, BNN6/DiR mixture solution (BD Sol), B-BD NAs, N-BD NAs, and T-BD NAs continued to rise to >50 °C within 10 min under laser irradiation, whereas the temperature of PBS (pH 7.4) did not dramatically change under the same conditions.

    Techniques:

    a Chemical structure of DiR, BNN6 (NO donor), and BPA (BNN6 precursor without NO production ability). b Co-assembly process of the binary NAs. c NO generation from B-BD NAs at various molar ratios of DiR and BNN6 from 10:1 to 1:10. Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. d – f Particle sizes distribution profiles and TEM images of B-BD NAs, T-BD NAs, and N-BD NAs, respectively, (Scale bar = 100 nm). Experiment was repeated three times independently with similar results. g Colloidal stability of B-BD NAs, T-BD NAs, and N-BD NAs incubated in PBS (pH 7.4) within 8 h at 37 ° C. Data are presented as mean ± SD ( n = 3, independent experiments). h In vitro thrombus-targeting fluorescence intensity in artificial blood clots treated with PBS (pH 7.4), DiR Sol, B-BD NAs, N-BD NAs, and T-BD NAs, respectively. Data are presented as mean ± SD ( n = 3, independent experiments). One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. The n.s. represent no significance. i Molecular docking simulation results of BNN6 and DiR. j Particle size changes of B-BD NAs and T-BD NAs in the presence of urea (50 mM), NaCl (50 mM), and SDS (50 mM), respectively ( n = 3, independent experiments). k UV absorption spectra of DiR Sol, BNN6 Sol, B-BD NAs, and T-BD NAs. l Fluorescence spectra of DiR Sol, B-BD NAs, and T-BD NAs.

    Journal: Nature Communications

    Article Title: Molecularly self‐fueled nano-penetrator for nonpharmaceutical treatment of thrombosis and ischemic stroke

    doi: 10.1038/s41467-023-35895-5

    Figure Lengend Snippet: a Chemical structure of DiR, BNN6 (NO donor), and BPA (BNN6 precursor without NO production ability). b Co-assembly process of the binary NAs. c NO generation from B-BD NAs at various molar ratios of DiR and BNN6 from 10:1 to 1:10. Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. d – f Particle sizes distribution profiles and TEM images of B-BD NAs, T-BD NAs, and N-BD NAs, respectively, (Scale bar = 100 nm). Experiment was repeated three times independently with similar results. g Colloidal stability of B-BD NAs, T-BD NAs, and N-BD NAs incubated in PBS (pH 7.4) within 8 h at 37 ° C. Data are presented as mean ± SD ( n = 3, independent experiments). h In vitro thrombus-targeting fluorescence intensity in artificial blood clots treated with PBS (pH 7.4), DiR Sol, B-BD NAs, N-BD NAs, and T-BD NAs, respectively. Data are presented as mean ± SD ( n = 3, independent experiments). One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. The n.s. represent no significance. i Molecular docking simulation results of BNN6 and DiR. j Particle size changes of B-BD NAs and T-BD NAs in the presence of urea (50 mM), NaCl (50 mM), and SDS (50 mM), respectively ( n = 3, independent experiments). k UV absorption spectra of DiR Sol, BNN6 Sol, B-BD NAs, and T-BD NAs. l Fluorescence spectra of DiR Sol, B-BD NAs, and T-BD NAs.

    Article Snippet: As shown in Fig. , the temperature of DiR Sol, BNN6/DiR mixture solution (BD Sol), B-BD NAs, N-BD NAs, and T-BD NAs continued to rise to >50 °C within 10 min under laser irradiation, whereas the temperature of PBS (pH 7.4) did not dramatically change under the same conditions.

    Techniques: Incubation, In Vitro, Fluorescence

    In vitro photothermal heating a image and b curves under laser irradiation (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3 independent experiments). Source data are provided as a Source Data file. c In vitro photothermal conversion efficiency of T-BD NAs. d NO release curves under laser irradiation (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. e Chemical mechanisms of NO generation from BNN6: intramolecular rearrangement and homogenous cleavage of N-NO bonds. f NO release curves of BNN6 Sol and T-BD NAs under heating (50 °C) or laser irradiation (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. g Off-on NO generation variations by the switch of laser irradiation (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. h Schematic representation of NO generation and autonomous motion features of carrier-free NAs and carrier-based NPs. i Laser-triggered NO release from T-BD NAs and PLGA-BD NAs at the same dose of DiR/BNN6 (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. j The movement trajectories of T-BD NAs and PLGA-BD NAs incubated in PBS (pH 7.4) at the same dose of DiR/BNN6 under laser irradiations (0 W/cm 2 and 2 W/cm 2 , 60 s), scale bar = 1 μm. k The motion speed of T-BD NAs and PLGA-BD NPs incubated in PBS (pH 7.4) at the same dose of DiR/BNN6 under laser irradiations (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. The n.s. represent no significance. Yellow lightning represents laser irradiation. L and H represent laser and heating, respectively.

    Journal: Nature Communications

    Article Title: Molecularly self‐fueled nano-penetrator for nonpharmaceutical treatment of thrombosis and ischemic stroke

    doi: 10.1038/s41467-023-35895-5

    Figure Lengend Snippet: In vitro photothermal heating a image and b curves under laser irradiation (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3 independent experiments). Source data are provided as a Source Data file. c In vitro photothermal conversion efficiency of T-BD NAs. d NO release curves under laser irradiation (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. e Chemical mechanisms of NO generation from BNN6: intramolecular rearrangement and homogenous cleavage of N-NO bonds. f NO release curves of BNN6 Sol and T-BD NAs under heating (50 °C) or laser irradiation (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. g Off-on NO generation variations by the switch of laser irradiation (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. h Schematic representation of NO generation and autonomous motion features of carrier-free NAs and carrier-based NPs. i Laser-triggered NO release from T-BD NAs and PLGA-BD NAs at the same dose of DiR/BNN6 (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. j The movement trajectories of T-BD NAs and PLGA-BD NAs incubated in PBS (pH 7.4) at the same dose of DiR/BNN6 under laser irradiations (0 W/cm 2 and 2 W/cm 2 , 60 s), scale bar = 1 μm. k The motion speed of T-BD NAs and PLGA-BD NPs incubated in PBS (pH 7.4) at the same dose of DiR/BNN6 under laser irradiations (2 W/cm 2 ). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. The n.s. represent no significance. Yellow lightning represents laser irradiation. L and H represent laser and heating, respectively.

    Article Snippet: As shown in Fig. , the temperature of DiR Sol, BNN6/DiR mixture solution (BD Sol), B-BD NAs, N-BD NAs, and T-BD NAs continued to rise to >50 °C within 10 min under laser irradiation, whereas the temperature of PBS (pH 7.4) did not dramatically change under the same conditions.

    Techniques: In Vitro, Irradiation, Incubation

    a Schematic representation of photothermal/mechanical synergistic thrombolysis and clot-deep penetration. b Photos of blood clots after different treatments with/without the 808 nm NIR laser irradiation: (2 W/cm 2 , 20 min). c Fibrin and hemoglobin levels in the blood clot supernatants after different treatments with/without the 808 nm NIR laser irradiation. Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. The n.s. represent no significance. d Images of blood clots before and after different treatments with 808 nm NIR laser irradiation (2 W/cm 2 , 20 min), scale bar = 2 mm. e H&E staining results of d under 808 nm NIR laser irradiation (2 W/cm 2 , 20 min), scale bar = 100 μm. f Thrombolytic rates of d under 808 nm NIR laser irradiation (2 W/cm 2 , 20 min). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. The n.s. represent no significance. g CLSM images of small red thrombi incubated with free C-6, C6/B-BD NAs, C6/N-BD NAs, or C6/T-BD NAs under 808 nm laser irradiation (2 W/cm 2 , 15 min), scale bar = 100 μm. Experiment was repeated three times independently with similar results. h Quantitative results of the depth of clot penetration. (I: PBS, II: BNN6 Sol, III: DiR Sol, IV: BD Sol, V: B-BD NAs, VI: T-BD NAs, VII: N-BD NAs). The lightning bolt represents laser irradiation.

    Journal: Nature Communications

    Article Title: Molecularly self‐fueled nano-penetrator for nonpharmaceutical treatment of thrombosis and ischemic stroke

    doi: 10.1038/s41467-023-35895-5

    Figure Lengend Snippet: a Schematic representation of photothermal/mechanical synergistic thrombolysis and clot-deep penetration. b Photos of blood clots after different treatments with/without the 808 nm NIR laser irradiation: (2 W/cm 2 , 20 min). c Fibrin and hemoglobin levels in the blood clot supernatants after different treatments with/without the 808 nm NIR laser irradiation. Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. The n.s. represent no significance. d Images of blood clots before and after different treatments with 808 nm NIR laser irradiation (2 W/cm 2 , 20 min), scale bar = 2 mm. e H&E staining results of d under 808 nm NIR laser irradiation (2 W/cm 2 , 20 min), scale bar = 100 μm. f Thrombolytic rates of d under 808 nm NIR laser irradiation (2 W/cm 2 , 20 min). Data are presented as mean ± SD ( n = 3, independent experiments). Source data are provided as a Source Data file. One-way ANOVA (one-sided) with Dunnett’s multiple comparisons test was used for the analysis of data and adjusted P value. The n.s. represent no significance. g CLSM images of small red thrombi incubated with free C-6, C6/B-BD NAs, C6/N-BD NAs, or C6/T-BD NAs under 808 nm laser irradiation (2 W/cm 2 , 15 min), scale bar = 100 μm. Experiment was repeated three times independently with similar results. h Quantitative results of the depth of clot penetration. (I: PBS, II: BNN6 Sol, III: DiR Sol, IV: BD Sol, V: B-BD NAs, VI: T-BD NAs, VII: N-BD NAs). The lightning bolt represents laser irradiation.

    Article Snippet: As shown in Fig. , the temperature of DiR Sol, BNN6/DiR mixture solution (BD Sol), B-BD NAs, N-BD NAs, and T-BD NAs continued to rise to >50 °C within 10 min under laser irradiation, whereas the temperature of PBS (pH 7.4) did not dramatically change under the same conditions.

    Techniques: Irradiation, Staining, Incubation