algorithms digitalmicrograph gatan (Gatan Inc)

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Gatan Inc algorithms digitalmicrograph gatan
Algorithms Digitalmicrograph Gatan, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 99/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/digitalmicrograph+software/Latitude+S+Software/pm40763734-198-44-46
Average 99 stars, based on 123 article reviews

algorithms digitalmicrograph gatan - by Bioz Stars, 2026-11

99/100 stars

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Article Title: Transcription activation mechanism of a noncanonical DNA damage response pathway by the WYL-activator, DriD
Article Snippet: For each data collection, the microscope was aligned using a cross-grating replica TEM grid; coma-free alignment and astigmatism corrections were done using the AutoCTF Sherpa functionality (Thermo Fisher Scientific). .. Data were collected using Latitude S [Digital Micrograph (Gatan) version 3.51] software running an image shift pattern at 0° stage tilt in counting mode with a nominal magnification of 81,000×/105,000× with a physical pixel size of 1.08/0.873 Å pixel and a defocus range of −0.8 to −2.5 μm. .. For grid preparation, UltrAufoil R1.2/1.3 Au 300 (Quantifoil) holey gold grids were glow-discharged (PELCO easiGlow) for 300 s, and 3 μl of purified samples of each three reconstituted complexes of DriD-ssDNA-RNAP-σ73-complexes with didA , CCNA_03891/CCNA_01149 , and bapE promoters were applied at room temperature at 90% humidity on separate grids.

Article Title: A neutralizing human antibody induces movement of the HCoV-229E receptor binding domain.
Article Snippet: Excess liquidwas blotted away for 5.5 s using aVitrobotMark IV (FEI) maintained at 4 °C and 100% humidity. .. Data were collected using a Titan Krios G3i (ThermoFisher) operating at 300 kV with a K3 Bioquantum detector (Gatan) using Latitude S automation software (Gatan). ..

Article Title: Transcription activation mechanism of a noncanonical DNA damage response pathway by the WYL-activator, DriD.
Article Snippet: For each data collection, the microscope was aligned using a crossgrating replica TEM grid; coma- free alignment and astigmatism corrections were done using the AutoCTF Sherpa functionality (Thermo Fisher Scientific). .. Data were collected using Latitude S [Digital Micrograph (Gatan) version 3.51] software running an image shift pattern at 0° stage tilt in counting mode with a nominal magnification of 81,000×/105,000× with a physical pixel size of 1.08/0.873 Å pixel and a defocus range of −0.8 to −2.5 μm. ..

Single Particle:

Article Title: Mechanism of beta-arrestin 1 mediated Src activation via Src SH3 domain revealed by cryo-electron microscopy.
Article Snippet: Data were analyzed using MicroCal Origin software to obtain thermodynamic parameters of binding and association constant (Ka=1/Kd). .. 60-frame Videos with a dose rate of ~15 electrons per pixel per second and a total accumulated dose of ~53-60 electrons per Å2 were collected using the Latitude-S (Gatan) single-particle data acquisition program. .. SH3–βarr1 was separated from unbound βarr1 by immobilized metal ion affinity chromatography using Talon resin (Takara Bio).

Article Title: Antiparallel stacking of Csu pili drives Acinetobacter baumannii 3D biofilm assembly
Article Snippet: The data were collected on a Titan Krios transmission electron microscope (Thermo Fisher) operating at 300 kV equipped with a K3 direct electron detector (Gatan) in counting mode with a BioQuantum GIF energy filter (slit width of 20 eV) at a magnification of × 81,000 corresponding to a pixel size of 1.08 Å at the specimen level. .. 60-frame videos with a dose rate of ~15 electrons per pixel per second and a total accumulated dose of 54.8 e-/Å 2 were collected using the Latitude-S (Gatan) single-particle data acquisition programme. ..

Article Title: Mechanism of beta-arrestin 1 mediated Src activation via Src SH3 domain revealed by cryo-electron microscopy
Article Snippet: The data were collected on a Titan Krios transmission electron microscope (Thermo Fisher) operating at 300 kV equipped with a K3 direct electron detector (Gatan) in counting mode with a BioQuantum GIF energy filter (slit width of 20 eV) at a magnification of ×81,000 corresponding to a pixel size of 1.08 Å at the specimen level. .. 60-frame Videos with a dose rate of ~15 electrons per pixel per second and a total accumulated dose of ~53-60 electrons per Å 2 were collected using the Latitude-S (Gatan) single-particle data acquisition program. .. Mutations were introduced using QuickChange II site-directed mutagenesis kit (Agilent) and verified by Sanger sequencing.

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Article Title: A highly potent and broadly accessible bispecific nanobody for the treatment of ebola virus infections.
Article Snippet: Grids were blotted for 4 seconds at 22°C under 100% chamber humidity and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI). .. Cryo-EM data were collected using a Latitude-S system (Gatan) equipped with a K3 direct electron detector and a Biocontinuum energy filter (Gatan). ..

Article Title: A highly potent and broadly accessible bispecific nanobody for the treatment of ebola virus infections
Article Snippet: Grids were blotted for 4 seconds at 22°C under 100% chamber humidity and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI). .. Cryo-EM data were collected using a Latitude-S system (Gatan) equipped with a K3 direct electron detector and a Biocontinuum energy filter (Gatan). ..

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