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diffusion pulse sequence bppled  (Bruker Corporation)


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    Bruker Corporation diffusion pulse sequence bppled
    Pulse sequence used for the 1 H NOE pumping experiments. This pulse sequence is essentially a <t>BPPLED</t> (Bipolar Pulse Pair Longitudinal Eddy current Delay) experiment including a T 2 -filter (CPMG block prior to acquisition) for suppressing macromolecule signals. Gradient blanking and the activation of the field-frequency lock were performed after the eddy current recovery delay for gradient pulse G 4 . Solvent presaturation was used during the relaxation delay when needed. Pulses : Narrow (wide) bars correspond to 90° (180°) hard rectangular pulses. Gradient pulses are represented by gray shapes denoted by G 1 –G 4 . G 1 and G 3 <t>are</t> <t>diffusion</t> gradients (1000 μs), and G 2 and G 4 are homospoil gradients (600 μs). Delays : Δ = diffusion time (250 ms), t mix = mixing time for NOE build-up (10–3000 ms), and τ = CPMG delay (242 μs). Total duration of the CPMG period is 4 Nτ , where N is the loop counter. Typical CPMG durations range from 50 to 100 ms. Phase cycle: ϕ 2 = x x −x −x; ϕ 3 = x x x x −x −x −x −x y y y y −y −y −y −y; ϕ 4 = x −x x −x −x x −x x y −y y −y −y y −y y; ϕ 5 = x x x x −x −x −x −x y y y y −y −y −y −y; ϕ 6 = y y y y y y y y −x −x −x −x −x −x −x −x; ϕ r = x −x −x x −x x x −x −y y y −y y −y −y y .
    Diffusion Pulse Sequence Bppled, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 97/100, based on 3073 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/diffusion+pulse+sequence+bppled/Diffusion/pmc12903051-68-3-2
    Average 97 stars, based on 3073 article reviews
    diffusion pulse sequence bppled - by Bioz Stars, 2026-09
    97/100 stars

    Images

    1) Product Images from "Revealing Organophosphorus and Carbamate Interactions with Albumin Using 1 H NOE Pumping NMR Technique"

    Article Title: Revealing Organophosphorus and Carbamate Interactions with Albumin Using 1 H NOE Pumping NMR Technique

    Journal: Analytical Chemistry

    doi: 10.1021/acs.analchem.5c02132

    Pulse sequence used for the 1 H NOE pumping experiments. This pulse sequence is essentially a BPPLED (Bipolar Pulse Pair Longitudinal Eddy current Delay) experiment including a T 2 -filter (CPMG block prior to acquisition) for suppressing macromolecule signals. Gradient blanking and the activation of the field-frequency lock were performed after the eddy current recovery delay for gradient pulse G 4 . Solvent presaturation was used during the relaxation delay when needed. Pulses : Narrow (wide) bars correspond to 90° (180°) hard rectangular pulses. Gradient pulses are represented by gray shapes denoted by G 1 –G 4 . G 1 and G 3 are diffusion gradients (1000 μs), and G 2 and G 4 are homospoil gradients (600 μs). Delays : Δ = diffusion time (250 ms), t mix = mixing time for NOE build-up (10–3000 ms), and τ = CPMG delay (242 μs). Total duration of the CPMG period is 4 Nτ , where N is the loop counter. Typical CPMG durations range from 50 to 100 ms. Phase cycle: ϕ 2 = x x −x −x; ϕ 3 = x x x x −x −x −x −x y y y y −y −y −y −y; ϕ 4 = x −x x −x −x x −x x y −y y −y −y y −y y; ϕ 5 = x x x x −x −x −x −x y y y y −y −y −y −y; ϕ 6 = y y y y y y y y −x −x −x −x −x −x −x −x; ϕ r = x −x −x x −x x x −x −y y y −y y −y −y y .
    Figure Legend Snippet: Pulse sequence used for the 1 H NOE pumping experiments. This pulse sequence is essentially a BPPLED (Bipolar Pulse Pair Longitudinal Eddy current Delay) experiment including a T 2 -filter (CPMG block prior to acquisition) for suppressing macromolecule signals. Gradient blanking and the activation of the field-frequency lock were performed after the eddy current recovery delay for gradient pulse G 4 . Solvent presaturation was used during the relaxation delay when needed. Pulses : Narrow (wide) bars correspond to 90° (180°) hard rectangular pulses. Gradient pulses are represented by gray shapes denoted by G 1 –G 4 . G 1 and G 3 are diffusion gradients (1000 μs), and G 2 and G 4 are homospoil gradients (600 μs). Delays : Δ = diffusion time (250 ms), t mix = mixing time for NOE build-up (10–3000 ms), and τ = CPMG delay (242 μs). Total duration of the CPMG period is 4 Nτ , where N is the loop counter. Typical CPMG durations range from 50 to 100 ms. Phase cycle: ϕ 2 = x x −x −x; ϕ 3 = x x x x −x −x −x −x y y y y −y −y −y −y; ϕ 4 = x −x x −x −x x −x x y −y y −y −y y −y y; ϕ 5 = x x x x −x −x −x −x y y y y −y −y −y −y; ϕ 6 = y y y y y y y y −x −x −x −x −x −x −x −x; ϕ r = x −x −x x −x x x −x −y y y −y y −y −y y .

    Techniques Used: Sequencing, Blocking Assay, Activation Assay, Solvent, Diffusion-based Assay

    Related Articles

    Mass Spectrometry:

    Article Title: Insights into Arcanobacterium haemolyticum : A Narrative Review of an Emerging Pathogen Revisited
    Article Snippet: .. Gu (2024) [ ] , Skin swab , MALDI-TOF Mass Spectrometry (Bruker) , Kirby Bauer disk diffusion test (Etest) , CLSI , PEN-susceptible. .. Lampejo (2024) [ ] , Blood culture , MALDI-TOF Mass Spectrometry , Kirby Bauer disk diffusion test , EUCAST , CLI-, PEN-, VA-susceptible. MTZ-resistant.

    Article Title: Insights into Arcanobacterium haemolyticum : A Narrative Review of an Emerging Pathogen Revisited.
    Article Snippet: .. Author Year Reference Type of Sample Method of Identification Method ofSusceptibility Testing Interpretation Criteria Antibiotics Resistance Phenotype (MIC mg/L) Chinello (2025) [28] Material drained from orbital cellulitis MALDI-TOF Mass Spectrometry NR NR AMC < 0.016, AZY 2, CLI > 256, DAPTO 4, LZD 0.19, MEM 0.012, MTZ > 256, TZP < 0.016 Faiz (2025) [29] Intraoperative sample from abscess MALDI-TOF Mass Spectrometry (Bruker) Kirby Bauer disk diffusion test EUCAST 2023 breakpoint for Corynebacterium CLI-, LIN-, RIF-, TET-susceptible CIP-, MOX-, PEN-, SXT-, VA-resistant Lovering (2025) [30] Blood culture MALDI-TOF MassSpectrometry NR NR CN, CRO, LZD, MEM, PEN, VA susceptible Saijo (2025) [31] Intraoperative sample from debridement MALDI-TOF Mass Spectrometry NR NR CRO 0.5, FEP 2, CTX 0.5, ERY < 0.12, PEN 0.25, RIF < 1, SXT < 0.5, VA 0.5 Bozso (2024) [32] Blood culture NR NR NR CLI ≤ 0.016, PEN ≤ 0.03 Chang (2024) [33] Blood culture, intraoperative samples from abscess NR NR NR NR Chin (2024) [34] Intraoperative sample from abscess NR NR NR NR Grusiecki (2024) [35] Purulent material from abscess NR NR NR PEN-susceptible CIP-, CLI-, MXF-resistant Gu (2024) [36] Skin swab MALDI-TOF Mass Spectrometry (Bruker) Kirby Bauer disk diffusion test (Etest) CLSI PEN-susceptible https://doi.org/10.3390/pathogens15030335 Table 3. ..

    Article Title: Insights into Arcanobacterium haemolyticum : A Narrative Review of an Emerging Pathogen Revisited.
    Article Snippet: .. MTZ-resistant Alrwashdeh (2023) [38] Blood culture and BAL MALDI-TOF MassSpectrometry NR PEN-susceptible Herai (2023) [39] Pleural fluid MALDI-TOF Mass Spectrometry (Bruker) NR NR NR Sahhar (2023) [40] Intraoperative sample from abscess MALDI-TOF Mass Spectrometry (Bruker) Kirby Bauer diffusion test (Disks and Etest) NR CLI-, CRO-, DOX-, LEV-, LZD-, PEN, RIF-, VA-susceptible Hicks (2022) [41] Intraoperative sample from abscess MALDI-TOF Mass Spectrometry Kirby Bauer diffusion test (Etest) NR PEN 0.064 Thomas (2022) [42] Intraoperative sample from debridement NR NR NR NR Purulent material from wound NR NR NR NR Purulent material from wound NR NR NR NR Intraoperative sample from debridement NR NR NR NR Intraoperative sample from debridement NR NR NR NR Purulent material from ulcer NR NR NR NR https://doi.org/10.3390/pathogens15030335 Table 3. ..

    Article Title: Insights into Arcanobacterium haemolyticum : A Narrative Review of an Emerging Pathogen Revisited
    Article Snippet: Herai (2023) [ ] , Pleural fluid , MALDI-TOF Mass Spectrometry (Bruker) , NR , NR , NR. .. Sahhar (2023) [ ] , Intraoperative sample from abscess , MALDI-TOF Mass Spectrometry (Bruker) , Kirby Bauer diffusion test (Disks and Etest) , NR , CLI-, CRO-, DOX-, LEV-, LZD-, PEN, RIF-, VA-susceptible. .. Hicks (2022) [ ] , Intraoperative sample from abscess , MALDI-TOF Mass Spectrometry , Kirby Bauer diffusion test (Etest) , NR , PEN 0.064.

    Article Title: Insights into Arcanobacterium haemolyticum : A Narrative Review of an Emerging Pathogen Revisited
    Article Snippet: Chinello (2025) [ ] , Material drained from orbital cellulitis , MALDI-TOF Mass Spectrometry , NR , NR , AMC < 0.016, AZY 2, CLI > 256, DAPTO 4, LZD 0.19, MEM 0.012, MTZ > 256, TZP < 0.016. .. Faiz (2025) [ ] , Intraoperative sample from abscess , MALDI-TOF Mass Spectrometry (Bruker) , Kirby Bauer disk diffusion test , EUCAST 2023 breakpoint for Corynebacterium , CLI-, LIN-, RIF-, TET-susceptible CIP-, MOX-, PEN-, SXT-, VA-resistant. .. Lovering (2025) [ ] , Blood culture , MALDI-TOF Mass Spectrometry , NR , NR , CN, CRO, LZD, MEM, PEN, VA susceptible.

    Diffusion-based Assay:

    Article Title: Insights into Arcanobacterium haemolyticum : A Narrative Review of an Emerging Pathogen Revisited
    Article Snippet: .. Gu (2024) [ ] , Skin swab , MALDI-TOF Mass Spectrometry (Bruker) , Kirby Bauer disk diffusion test (Etest) , CLSI , PEN-susceptible. .. Lampejo (2024) [ ] , Blood culture , MALDI-TOF Mass Spectrometry , Kirby Bauer disk diffusion test , EUCAST , CLI-, PEN-, VA-susceptible. MTZ-resistant.

    Article Title: Potassium Hexafluoroacetylacetonate Complex with 18-Crown-6 Ether as a Volatile Precursor of Molecular and Inorganic Films: Thermal and Structural Insights.
    Article Snippet: The 1H DOSY NMR experiment [85,86] was performed on the same equipment with a 5 mm BBI probe with z-gradient. .. Diffusion coefficients were measured using the standard Bruker Ledbpgp2s sequence. ..

    Article Title: Insights into Arcanobacterium haemolyticum : A Narrative Review of an Emerging Pathogen Revisited.
    Article Snippet: .. Author Year Reference Type of Sample Method of Identification Method ofSusceptibility Testing Interpretation Criteria Antibiotics Resistance Phenotype (MIC mg/L) Chinello (2025) [28] Material drained from orbital cellulitis MALDI-TOF Mass Spectrometry NR NR AMC < 0.016, AZY 2, CLI > 256, DAPTO 4, LZD 0.19, MEM 0.012, MTZ > 256, TZP < 0.016 Faiz (2025) [29] Intraoperative sample from abscess MALDI-TOF Mass Spectrometry (Bruker) Kirby Bauer disk diffusion test EUCAST 2023 breakpoint for Corynebacterium CLI-, LIN-, RIF-, TET-susceptible CIP-, MOX-, PEN-, SXT-, VA-resistant Lovering (2025) [30] Blood culture MALDI-TOF MassSpectrometry NR NR CN, CRO, LZD, MEM, PEN, VA susceptible Saijo (2025) [31] Intraoperative sample from debridement MALDI-TOF Mass Spectrometry NR NR CRO 0.5, FEP 2, CTX 0.5, ERY < 0.12, PEN 0.25, RIF < 1, SXT < 0.5, VA 0.5 Bozso (2024) [32] Blood culture NR NR NR CLI ≤ 0.016, PEN ≤ 0.03 Chang (2024) [33] Blood culture, intraoperative samples from abscess NR NR NR NR Chin (2024) [34] Intraoperative sample from abscess NR NR NR NR Grusiecki (2024) [35] Purulent material from abscess NR NR NR PEN-susceptible CIP-, CLI-, MXF-resistant Gu (2024) [36] Skin swab MALDI-TOF Mass Spectrometry (Bruker) Kirby Bauer disk diffusion test (Etest) CLSI PEN-susceptible https://doi.org/10.3390/pathogens15030335 Table 3. ..

    Article Title: Insights into Arcanobacterium haemolyticum : A Narrative Review of an Emerging Pathogen Revisited.
    Article Snippet: .. MTZ-resistant Alrwashdeh (2023) [38] Blood culture and BAL MALDI-TOF MassSpectrometry NR PEN-susceptible Herai (2023) [39] Pleural fluid MALDI-TOF Mass Spectrometry (Bruker) NR NR NR Sahhar (2023) [40] Intraoperative sample from abscess MALDI-TOF Mass Spectrometry (Bruker) Kirby Bauer diffusion test (Disks and Etest) NR CLI-, CRO-, DOX-, LEV-, LZD-, PEN, RIF-, VA-susceptible Hicks (2022) [41] Intraoperative sample from abscess MALDI-TOF Mass Spectrometry Kirby Bauer diffusion test (Etest) NR PEN 0.064 Thomas (2022) [42] Intraoperative sample from debridement NR NR NR NR Purulent material from wound NR NR NR NR Purulent material from wound NR NR NR NR Intraoperative sample from debridement NR NR NR NR Intraoperative sample from debridement NR NR NR NR Purulent material from ulcer NR NR NR NR https://doi.org/10.3390/pathogens15030335 Table 3. ..

    Article Title: Insights into Arcanobacterium haemolyticum : A Narrative Review of an Emerging Pathogen Revisited
    Article Snippet: Herai (2023) [ ] , Pleural fluid , MALDI-TOF Mass Spectrometry (Bruker) , NR , NR , NR. .. Sahhar (2023) [ ] , Intraoperative sample from abscess , MALDI-TOF Mass Spectrometry (Bruker) , Kirby Bauer diffusion test (Disks and Etest) , NR , CLI-, CRO-, DOX-, LEV-, LZD-, PEN, RIF-, VA-susceptible. .. Hicks (2022) [ ] , Intraoperative sample from abscess , MALDI-TOF Mass Spectrometry , Kirby Bauer diffusion test (Etest) , NR , PEN 0.064.

    Article Title: Physical and chemical characterisation of ophthalmic lens-grinding wastewater: uncovering environmental implications.
    Article Snippet: .. 1D 1H and 2D 1H-1H NMR DOSY (Diffusion-ordered spectroscopy) experiments were carried out on a 9.4 T Bruker AVANCE III 400 spectrometer (Bruker Corporation, Billerica MA, USA) equipped with a Bruker DiffBB probe, specifically a 5 mm high-power diffusion BBI probe. ..

    Article Title: Insights into Arcanobacterium haemolyticum : A Narrative Review of an Emerging Pathogen Revisited
    Article Snippet: Chinello (2025) [ ] , Material drained from orbital cellulitis , MALDI-TOF Mass Spectrometry , NR , NR , AMC < 0.016, AZY 2, CLI > 256, DAPTO 4, LZD 0.19, MEM 0.012, MTZ > 256, TZP < 0.016. .. Faiz (2025) [ ] , Intraoperative sample from abscess , MALDI-TOF Mass Spectrometry (Bruker) , Kirby Bauer disk diffusion test , EUCAST 2023 breakpoint for Corynebacterium , CLI-, LIN-, RIF-, TET-susceptible CIP-, MOX-, PEN-, SXT-, VA-resistant. .. Lovering (2025) [ ] , Blood culture , MALDI-TOF Mass Spectrometry , NR , NR , CN, CRO, LZD, MEM, PEN, VA susceptible.

    Sequencing:

    Article Title: Potassium Hexafluoroacetylacetonate Complex with 18-Crown-6 Ether as a Volatile Precursor of Molecular and Inorganic Films: Thermal and Structural Insights.
    Article Snippet: The 1H DOSY NMR experiment [85,86] was performed on the same equipment with a 5 mm BBI probe with z-gradient. .. Diffusion coefficients were measured using the standard Bruker Ledbpgp2s sequence. ..

    Nuclear Magnetic Resonance:

    Article Title: Physical and chemical characterisation of ophthalmic lens-grinding wastewater: uncovering environmental implications.
    Article Snippet: .. 1D 1H and 2D 1H-1H NMR DOSY (Diffusion-ordered spectroscopy) experiments were carried out on a 9.4 T Bruker AVANCE III 400 spectrometer (Bruker Corporation, Billerica MA, USA) equipped with a Bruker DiffBB probe, specifically a 5 mm high-power diffusion BBI probe. ..

    Spectroscopy:

    Article Title: Physical and chemical characterisation of ophthalmic lens-grinding wastewater: uncovering environmental implications.
    Article Snippet: .. 1D 1H and 2D 1H-1H NMR DOSY (Diffusion-ordered spectroscopy) experiments were carried out on a 9.4 T Bruker AVANCE III 400 spectrometer (Bruker Corporation, Billerica MA, USA) equipped with a Bruker DiffBB probe, specifically a 5 mm high-power diffusion BBI probe. ..



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