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Medion Diagnostics diff-quik® fix stained smear
Diff Quik® Fix Stained Smear, supplied by Medion Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diff-quik+fixative/diff+quik/pm32602953-52-6-9
Average 90 stars, based on 1 article reviews
diff-quik® fix stained smear - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Staining:

Article Title: Beyond the microcirculation: sequestration of infected red blood cells and reduced flow in large draining veins in experimental cerebral malaria
Article Snippet: Parasitemia was measured daily after infection by microscopic examination of Diff-Quik (Medion Grifols Diagnostics, Switzerland) -stained thin blood smears.

Article Title: The Toxoplasma rhoptry protein ROP55 is a major virulence factor that prevents lytic host cell death.
Article Snippet: On day 6 post-infection, cells were fixed with 4% paraformaldehyde (PFA) (w/v) for 20min, and stained with Diff-Quik dyes (Medion Diagnostics).

Article Title: Genetic Damage and Multi-Elemental Exposure in Populations in Proximity to Artisanal and Small-Scale Gold (ASGM) Mining Areas in North Colombia.
Article Snippet: Lymphocytes were then collected by centrifugation at 1200 rpm for 8 min, fixed in methanol/acetic acid solution (25:1, v/v), mounted on clean slides and stained with Diff-Quik (Medion Diagnostics, Düdingen, Switzerland).

Article Title: The Toxoplasma rhoptry protein ROP55 is a major virulence factor that prevents lytic host cell death
Article Snippet: On day 6 post-infection, cells were fixed with 4% paraformaldehyde (PFA) (w/v) for 20 min, and stained with Diff-Quik dyes (Medion Diagnostics).

Article Title: Type V collagen-induced nasal tolerance prevents lung damage in an experimental model: new evidence of autoimmunity to collagen V in COPD
Article Snippet: Aliquots of the cell suspension were centrifuged on glass slides using a cytocentrifuge and stained with Diff-Quik (Medion Diagnostics, Dündingen, Switzerland).

Article Title: Culture and Immunomodulation of Equine Muscle-Derived Mesenchymal Stromal Cells: A Comparative Study of Innovative 2D versus 3D Models Using Equine Platelet Lysate
Article Snippet: Smears of neutrophils isolated from horses were placed on microscope slides, stained by Diff-Quik (Medion Diagnostics, Freiburg, Germany), and observed by microscope (Zeiss, Axioskop, Zaventem, Belgium).

Article Title: Impact of administration route and PEGylation on alpha-1 antitrypsin augmentation therapy.
Article Snippet: Patients suffering from emphysema associated with alpha-1 antitrypsin (AAT) deficiency can benefit from augmentation therapy.. AAT is administered to the patient once a week via intravenous infusion by a healthcare professional.. However, only 2 % of the AAT dose reaches the lungs following intravenous infusion.

Article Title: Genetic Damage and Multi-Elemental Exposure in Populations in Proximity to Artisanal and Small-Scale Gold (ASGM) Mining Areas in North Colombia
Article Snippet: Lymphocytes were then collected by centrifugation at 1200 rpm for 8 min, fixed in methanol/acetic acid solution (25:1, v / v ), mounted on clean slides and stained with Diff-Quik (Medion Diagnostics, Düdingen, Switzerland).

Diff-Quik:

Article Title: Beyond the microcirculation: sequestration of infected red blood cells and reduced flow in large draining veins in experimental cerebral malaria
Article Snippet: Parasitemia was measured daily after infection by microscopic examination of Diff-Quik (Medion Grifols Diagnostics, Switzerland) -stained thin blood smears.

Article Title: The Toxoplasma rhoptry protein ROP55 is a major virulence factor that prevents lytic host cell death.
Article Snippet: On day 6 post-infection, cells were fixed with 4% paraformaldehyde (PFA) (w/v) for 20min, and stained with Diff-Quik dyes (Medion Diagnostics).

Article Title: Genetic Damage and Multi-Elemental Exposure in Populations in Proximity to Artisanal and Small-Scale Gold (ASGM) Mining Areas in North Colombia.
Article Snippet: Lymphocytes were then collected by centrifugation at 1200 rpm for 8 min, fixed in methanol/acetic acid solution (25:1, v/v), mounted on clean slides and stained with Diff-Quik (Medion Diagnostics, Düdingen, Switzerland).

Article Title: The Toxoplasma rhoptry protein ROP55 is a major virulence factor that prevents lytic host cell death
Article Snippet: On day 6 post-infection, cells were fixed with 4% paraformaldehyde (PFA) (w/v) for 20 min, and stained with Diff-Quik dyes (Medion Diagnostics).

Article Title: Type V collagen-induced nasal tolerance prevents lung damage in an experimental model: new evidence of autoimmunity to collagen V in COPD
Article Snippet: Aliquots of the cell suspension were centrifuged on glass slides using a cytocentrifuge and stained with Diff-Quik (Medion Diagnostics, Dündingen, Switzerland).

Article Title: Culture and Immunomodulation of Equine Muscle-Derived Mesenchymal Stromal Cells: A Comparative Study of Innovative 2D versus 3D Models Using Equine Platelet Lysate
Article Snippet: Smears of neutrophils isolated from horses were placed on microscope slides, stained by Diff-Quik (Medion Diagnostics, Freiburg, Germany), and observed by microscope (Zeiss, Axioskop, Zaventem, Belgium).

Article Title: Impact of administration route and PEGylation on alpha-1 antitrypsin augmentation therapy.
Article Snippet: Patients suffering from emphysema associated with alpha-1 antitrypsin (AAT) deficiency can benefit from augmentation therapy.. AAT is administered to the patient once a week via intravenous infusion by a healthcare professional.. However, only 2 % of the AAT dose reaches the lungs following intravenous infusion.

Article Title: Genetic Damage and Multi-Elemental Exposure in Populations in Proximity to Artisanal and Small-Scale Gold (ASGM) Mining Areas in North Colombia
Article Snippet: Lymphocytes were then collected by centrifugation at 1200 rpm for 8 min, fixed in methanol/acetic acid solution (25:1, v / v ), mounted on clean slides and stained with Diff-Quik (Medion Diagnostics, Düdingen, Switzerland).

Centrifugation:

Article Title: Beyond the microcirculation: sequestration of infected red blood cells and reduced flow in large draining veins in experimental cerebral malaria
Article Snippet: Parasitemia was measured daily after infection by microscopic examination of Diff-Quik (Medion Grifols Diagnostics, Switzerland) -stained thin blood smears.

Article Title: The Toxoplasma rhoptry protein ROP55 is a major virulence factor that prevents lytic host cell death.
Article Snippet: On day 6 post-infection, cells were fixed with 4% paraformaldehyde (PFA) (w/v) for 20min, and stained with Diff-Quik dyes (Medion Diagnostics).

Article Title: Genetic Damage and Multi-Elemental Exposure in Populations in Proximity to Artisanal and Small-Scale Gold (ASGM) Mining Areas in North Colombia.
Article Snippet: Lymphocytes were then collected by centrifugation at 1200 rpm for 8 min, fixed in methanol/acetic acid solution (25:1, v/v), mounted on clean slides and stained with Diff-Quik (Medion Diagnostics, Düdingen, Switzerland).

Article Title: The Toxoplasma rhoptry protein ROP55 is a major virulence factor that prevents lytic host cell death
Article Snippet: On day 6 post-infection, cells were fixed with 4% paraformaldehyde (PFA) (w/v) for 20 min, and stained with Diff-Quik dyes (Medion Diagnostics).

Article Title: Type V collagen-induced nasal tolerance prevents lung damage in an experimental model: new evidence of autoimmunity to collagen V in COPD
Article Snippet: Aliquots of the cell suspension were centrifuged on glass slides using a cytocentrifuge and stained with Diff-Quik (Medion Diagnostics, Dündingen, Switzerland).

Article Title: Culture and Immunomodulation of Equine Muscle-Derived Mesenchymal Stromal Cells: A Comparative Study of Innovative 2D versus 3D Models Using Equine Platelet Lysate
Article Snippet: Smears of neutrophils isolated from horses were placed on microscope slides, stained by Diff-Quik (Medion Diagnostics, Freiburg, Germany), and observed by microscope (Zeiss, Axioskop, Zaventem, Belgium).

Article Title: Impact of administration route and PEGylation on alpha-1 antitrypsin augmentation therapy.
Article Snippet: Patients suffering from emphysema associated with alpha-1 antitrypsin (AAT) deficiency can benefit from augmentation therapy.. AAT is administered to the patient once a week via intravenous infusion by a healthcare professional.. However, only 2 % of the AAT dose reaches the lungs following intravenous infusion.

Article Title: Genetic Damage and Multi-Elemental Exposure in Populations in Proximity to Artisanal and Small-Scale Gold (ASGM) Mining Areas in North Colombia
Article Snippet: Lymphocytes were then collected by centrifugation at 1200 rpm for 8 min, fixed in methanol/acetic acid solution (25:1, v / v ), mounted on clean slides and stained with Diff-Quik (Medion Diagnostics, Düdingen, Switzerland).

Suspension:

Article Title: Beyond the microcirculation: sequestration of infected red blood cells and reduced flow in large draining veins in experimental cerebral malaria
Article Snippet: Parasitemia was measured daily after infection by microscopic examination of Diff-Quik (Medion Grifols Diagnostics, Switzerland) -stained thin blood smears.

Article Title: The Toxoplasma rhoptry protein ROP55 is a major virulence factor that prevents lytic host cell death.
Article Snippet: On day 6 post-infection, cells were fixed with 4% paraformaldehyde (PFA) (w/v) for 20min, and stained with Diff-Quik dyes (Medion Diagnostics).

Article Title: Genetic Damage and Multi-Elemental Exposure in Populations in Proximity to Artisanal and Small-Scale Gold (ASGM) Mining Areas in North Colombia.
Article Snippet: Lymphocytes were then collected by centrifugation at 1200 rpm for 8 min, fixed in methanol/acetic acid solution (25:1, v/v), mounted on clean slides and stained with Diff-Quik (Medion Diagnostics, Düdingen, Switzerland).

Article Title: The Toxoplasma rhoptry protein ROP55 is a major virulence factor that prevents lytic host cell death
Article Snippet: On day 6 post-infection, cells were fixed with 4% paraformaldehyde (PFA) (w/v) for 20 min, and stained with Diff-Quik dyes (Medion Diagnostics).

Article Title: Type V collagen-induced nasal tolerance prevents lung damage in an experimental model: new evidence of autoimmunity to collagen V in COPD
Article Snippet: Aliquots of the cell suspension were centrifuged on glass slides using a cytocentrifuge and stained with Diff-Quik (Medion Diagnostics, Dündingen, Switzerland).

Article Title: Culture and Immunomodulation of Equine Muscle-Derived Mesenchymal Stromal Cells: A Comparative Study of Innovative 2D versus 3D Models Using Equine Platelet Lysate
Article Snippet: Smears of neutrophils isolated from horses were placed on microscope slides, stained by Diff-Quik (Medion Diagnostics, Freiburg, Germany), and observed by microscope (Zeiss, Axioskop, Zaventem, Belgium).

Article Title: Impact of administration route and PEGylation on alpha-1 antitrypsin augmentation therapy.
Article Snippet: Patients suffering from emphysema associated with alpha-1 antitrypsin (AAT) deficiency can benefit from augmentation therapy.. AAT is administered to the patient once a week via intravenous infusion by a healthcare professional.. However, only 2 % of the AAT dose reaches the lungs following intravenous infusion.

Article Title: Genetic Damage and Multi-Elemental Exposure in Populations in Proximity to Artisanal and Small-Scale Gold (ASGM) Mining Areas in North Colombia
Article Snippet: Lymphocytes were then collected by centrifugation at 1200 rpm for 8 min, fixed in methanol/acetic acid solution (25:1, v / v ), mounted on clean slides and stained with Diff-Quik (Medion Diagnostics, Düdingen, Switzerland).

Isolation:

Article Title: Beyond the microcirculation: sequestration of infected red blood cells and reduced flow in large draining veins in experimental cerebral malaria
Article Snippet: Parasitemia was measured daily after infection by microscopic examination of Diff-Quik (Medion Grifols Diagnostics, Switzerland) -stained thin blood smears.

Article Title: The Toxoplasma rhoptry protein ROP55 is a major virulence factor that prevents lytic host cell death.
Article Snippet: On day 6 post-infection, cells were fixed with 4% paraformaldehyde (PFA) (w/v) for 20min, and stained with Diff-Quik dyes (Medion Diagnostics).

Article Title: Genetic Damage and Multi-Elemental Exposure in Populations in Proximity to Artisanal and Small-Scale Gold (ASGM) Mining Areas in North Colombia.
Article Snippet: Lymphocytes were then collected by centrifugation at 1200 rpm for 8 min, fixed in methanol/acetic acid solution (25:1, v/v), mounted on clean slides and stained with Diff-Quik (Medion Diagnostics, Düdingen, Switzerland).

Article Title: The Toxoplasma rhoptry protein ROP55 is a major virulence factor that prevents lytic host cell death
Article Snippet: On day 6 post-infection, cells were fixed with 4% paraformaldehyde (PFA) (w/v) for 20 min, and stained with Diff-Quik dyes (Medion Diagnostics).

Article Title: Type V collagen-induced nasal tolerance prevents lung damage in an experimental model: new evidence of autoimmunity to collagen V in COPD
Article Snippet: Aliquots of the cell suspension were centrifuged on glass slides using a cytocentrifuge and stained with Diff-Quik (Medion Diagnostics, Dündingen, Switzerland).

Article Title: Culture and Immunomodulation of Equine Muscle-Derived Mesenchymal Stromal Cells: A Comparative Study of Innovative 2D versus 3D Models Using Equine Platelet Lysate
Article Snippet: Smears of neutrophils isolated from horses were placed on microscope slides, stained by Diff-Quik (Medion Diagnostics, Freiburg, Germany), and observed by microscope (Zeiss, Axioskop, Zaventem, Belgium).

Article Title: Impact of administration route and PEGylation on alpha-1 antitrypsin augmentation therapy.
Article Snippet: Patients suffering from emphysema associated with alpha-1 antitrypsin (AAT) deficiency can benefit from augmentation therapy.. AAT is administered to the patient once a week via intravenous infusion by a healthcare professional.. However, only 2 % of the AAT dose reaches the lungs following intravenous infusion.

Article Title: Genetic Damage and Multi-Elemental Exposure in Populations in Proximity to Artisanal and Small-Scale Gold (ASGM) Mining Areas in North Colombia
Article Snippet: Lymphocytes were then collected by centrifugation at 1200 rpm for 8 min, fixed in methanol/acetic acid solution (25:1, v / v ), mounted on clean slides and stained with Diff-Quik (Medion Diagnostics, Düdingen, Switzerland).

Microscopy:

Article Title: Beyond the microcirculation: sequestration of infected red blood cells and reduced flow in large draining veins in experimental cerebral malaria
Article Snippet: Parasitemia was measured daily after infection by microscopic examination of Diff-Quik (Medion Grifols Diagnostics, Switzerland) -stained thin blood smears.

Article Title: The Toxoplasma rhoptry protein ROP55 is a major virulence factor that prevents lytic host cell death.
Article Snippet: On day 6 post-infection, cells were fixed with 4% paraformaldehyde (PFA) (w/v) for 20min, and stained with Diff-Quik dyes (Medion Diagnostics).

Article Title: Genetic Damage and Multi-Elemental Exposure in Populations in Proximity to Artisanal and Small-Scale Gold (ASGM) Mining Areas in North Colombia.
Article Snippet: Lymphocytes were then collected by centrifugation at 1200 rpm for 8 min, fixed in methanol/acetic acid solution (25:1, v/v), mounted on clean slides and stained with Diff-Quik (Medion Diagnostics, Düdingen, Switzerland).

Article Title: The Toxoplasma rhoptry protein ROP55 is a major virulence factor that prevents lytic host cell death
Article Snippet: On day 6 post-infection, cells were fixed with 4% paraformaldehyde (PFA) (w/v) for 20 min, and stained with Diff-Quik dyes (Medion Diagnostics).

Article Title: Type V collagen-induced nasal tolerance prevents lung damage in an experimental model: new evidence of autoimmunity to collagen V in COPD
Article Snippet: Aliquots of the cell suspension were centrifuged on glass slides using a cytocentrifuge and stained with Diff-Quik (Medion Diagnostics, Dündingen, Switzerland).

Article Title: Culture and Immunomodulation of Equine Muscle-Derived Mesenchymal Stromal Cells: A Comparative Study of Innovative 2D versus 3D Models Using Equine Platelet Lysate
Article Snippet: Smears of neutrophils isolated from horses were placed on microscope slides, stained by Diff-Quik (Medion Diagnostics, Freiburg, Germany), and observed by microscope (Zeiss, Axioskop, Zaventem, Belgium).

Article Title: Impact of administration route and PEGylation on alpha-1 antitrypsin augmentation therapy.
Article Snippet: Patients suffering from emphysema associated with alpha-1 antitrypsin (AAT) deficiency can benefit from augmentation therapy.. AAT is administered to the patient once a week via intravenous infusion by a healthcare professional.. However, only 2 % of the AAT dose reaches the lungs following intravenous infusion.

Article Title: Genetic Damage and Multi-Elemental Exposure in Populations in Proximity to Artisanal and Small-Scale Gold (ASGM) Mining Areas in North Colombia
Article Snippet: Lymphocytes were then collected by centrifugation at 1200 rpm for 8 min, fixed in methanol/acetic acid solution (25:1, v / v ), mounted on clean slides and stained with Diff-Quik (Medion Diagnostics, Düdingen, Switzerland).

Infection:

Article Title: Beyond the microcirculation: sequestration of infected red blood cells and reduced flow in large draining veins in experimental cerebral malaria
Article Snippet: Parasitemia was measured daily after infection by microscopic examination of Diff-Quik (Medion Grifols Diagnostics, Switzerland) -stained thin blood smears.

Article Title: The Toxoplasma rhoptry protein ROP55 is a major virulence factor that prevents lytic host cell death.
Article Snippet: On day 6 post-infection, cells were fixed with 4% paraformaldehyde (PFA) (w/v) for 20min, and stained with Diff-Quik dyes (Medion Diagnostics).

Article Title: Genetic Damage and Multi-Elemental Exposure in Populations in Proximity to Artisanal and Small-Scale Gold (ASGM) Mining Areas in North Colombia.
Article Snippet: Lymphocytes were then collected by centrifugation at 1200 rpm for 8 min, fixed in methanol/acetic acid solution (25:1, v/v), mounted on clean slides and stained with Diff-Quik (Medion Diagnostics, Düdingen, Switzerland).

Article Title: The Toxoplasma rhoptry protein ROP55 is a major virulence factor that prevents lytic host cell death
Article Snippet: On day 6 post-infection, cells were fixed with 4% paraformaldehyde (PFA) (w/v) for 20 min, and stained with Diff-Quik dyes (Medion Diagnostics).

Article Title: Type V collagen-induced nasal tolerance prevents lung damage in an experimental model: new evidence of autoimmunity to collagen V in COPD
Article Snippet: Aliquots of the cell suspension were centrifuged on glass slides using a cytocentrifuge and stained with Diff-Quik (Medion Diagnostics, Dündingen, Switzerland).

Article Title: Culture and Immunomodulation of Equine Muscle-Derived Mesenchymal Stromal Cells: A Comparative Study of Innovative 2D versus 3D Models Using Equine Platelet Lysate
Article Snippet: Smears of neutrophils isolated from horses were placed on microscope slides, stained by Diff-Quik (Medion Diagnostics, Freiburg, Germany), and observed by microscope (Zeiss, Axioskop, Zaventem, Belgium).

Article Title: Impact of administration route and PEGylation on alpha-1 antitrypsin augmentation therapy.
Article Snippet: Patients suffering from emphysema associated with alpha-1 antitrypsin (AAT) deficiency can benefit from augmentation therapy.. AAT is administered to the patient once a week via intravenous infusion by a healthcare professional.. However, only 2 % of the AAT dose reaches the lungs following intravenous infusion.

Article Title: Genetic Damage and Multi-Elemental Exposure in Populations in Proximity to Artisanal and Small-Scale Gold (ASGM) Mining Areas in North Colombia
Article Snippet: Lymphocytes were then collected by centrifugation at 1200 rpm for 8 min, fixed in methanol/acetic acid solution (25:1, v / v ), mounted on clean slides and stained with Diff-Quik (Medion Diagnostics, Düdingen, Switzerland).



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Activation of phagocytosis by <t>NETs.</t> <t>Neutrophils</t> were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of <t>Giemsa-stained</t> neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.
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Activation of phagocytosis by <t>NETs.</t> <t>Neutrophils</t> were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of <t>Giemsa-stained</t> neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.
Diff Quik Fixative, supplied by Dade Behring, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diff-quik+fixative/diff+quik+stain+set/pm31217510-93-15-24
Average 90 stars, based on 1 article reviews
diff-quik fixative - by Bioz Stars, 2026-10
90/100 stars
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Activation of phagocytosis by NETs. Neutrophils were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of Giemsa-stained neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.

Journal: Frontiers in Immunology

Article Title: Neutrophil Extracellular Traps Activate Proinflammatory Functions of Human Neutrophils

doi: 10.3389/fimmu.2021.636954

Figure Lengend Snippet: Activation of phagocytosis by NETs. Neutrophils were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of Giemsa-stained neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.

Article Snippet: The preparations contained >99% granulocytes, of which >96% were neutrophils and 1%–4% were eosinophils, as determined by Giemsa staining (Diff Quik ® Fix, Medion Diagnostics, Berlin, Germany) of cytocentrifuged (Shandon) samples ( ).

Techniques: Activation Assay, Flow Cytometry, Staining, Fluorescence, Microscopy