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PanReac AppliChem diff-quick stain
Diff Quick Stain, supplied by PanReac AppliChem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diff+quick+stain+solution/diff+quick+staining/pm11272493-31-7-9
Average 90 stars, based on 1 article reviews
diff-quick stain - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Nebulized Heparin Attenuates Pulmonary Coagulopathy and Inflammation through Alveolar Macrophages in a Rat Model of Acute Lung Injury
Article Snippet: Cells were counted using a haemocytometer (Neubauer, Marienfeld, Lauda-Königshofen, Germany), and slides were prepared by cytocentrifugation (Shandon Cytospin 4, Thermo Electron Corporation, Marietta, OH) and Diff-Quick staining (Panreac Quimica SAU; Castellar del Vallès, Spain).

Article Title: Methotrexate Ameliorates Systemic Inflammation and Septic Associated-Lung Damage in a Cecal Ligation and Puncture Septic Rat Model
Article Snippet: The cell yield and purity were assessed by Diff-Quick staining (Panreac, Castellar del Vallés, Spain).

Article Title: Effects of nebulized antithrombin and heparin on inflammatory and coagulation alterations in an acute lung injury model in rats
Article Snippet: To determine the proportion of each cellular type in lavage fluids, slides with 200 000 cells were prepared by cytocentrifugation (Shandon Cytospin 4, Thermo Electron Corporation) and Diff‐Quick staining performed (Panreac Quimica SAU).

Article Title: Morphologic and Cytochemical Characteristics of the Blood Cells of the Yellow-Bellied Slider (Trachemys scripta scripta).
Article Snippet: Two blood smears of each turtle were stained with a quick Romanowsky-type stain (Diff-Quick, Panreac Qu ımica SAU, Barcelona, Spain; Casal and Or os, 2007).

Article Title: Priming Mesenchymal Stem Cells with Lipopolysaccharide Boosts the Immunomodulatory and Regenerative Activity of Secreted Extracellular Vesicles
Article Snippet: The differential cell count in BAL was also evaluated in cytospin preparations stained with Diff-Quick Kit (Panreac Quimica SAU, ref. 254101, 253999, 253998), according to manufacturer’s protocol.

Staining:

Article Title: Dietary supplementation of Bacillus velezensis improves Vibrio anguillarum clearance in European sea bass by activating essential innate immune mechanisms.
Article Snippet: .. After washing with PBS, the cells were stained with Diff Quick solution (Panreac, Spain). ..

Article Title: Sperm DNA fragmentation as a result of ultra-endurance exercise training in male athletes.
Article Snippet: 1Morphological Sciences Department, School of Medicine, University of Cordoba, Cordoba, Spain 2International Network on Physical Exercise and Fertility (INPEF), Cordoba, Spain 3Nutrir.. Nutrición Médica y Ejercicio, Cordoba, Spain 4Institute for Memory Impairments and Neurological Disorders (MIND Institute) University of California, Irvine 5Physical Education Department, School of Physical Activity and Sport Sciences, University of Las Palmas de Gran Canarias, Las Palmas, Spain

Article Title: Supplementation of arachidonic acid rich oil in European sea bass juveniles (Dicentrarchus labrax) diets: Effects on leucocytes and plasma fatty acid profiles, selected immune parameters and circulating prostaglandins levels.
Article Snippet: The main objective of this study was to assess the effects of graded levels of dietary arachidonic acid (ARA), supplemented from alternative sources, on fatty acid composition of plasma and head kidney leucocytes of European sea bass (Dicentrarchus labrax).. For that purpose, sea bass juveniles were fed four diets containing graded levels of ARA as follows: 0.5% (ARA0.5), 1% (ARA1), 2% (ARA2) and 4% (ARA4) during 60 days.. At the end of the feeding trial fatty acid profiles of plasma and head kidney leucocytes were analyzed.

Diff-Quik:

Article Title: Dietary supplementation of Bacillus velezensis improves Vibrio anguillarum clearance in European sea bass by activating essential innate immune mechanisms.
Article Snippet: .. After washing with PBS, the cells were stained with Diff Quick solution (Panreac, Spain). ..

Article Title: Sperm DNA fragmentation as a result of ultra-endurance exercise training in male athletes.
Article Snippet: 1Morphological Sciences Department, School of Medicine, University of Cordoba, Cordoba, Spain 2International Network on Physical Exercise and Fertility (INPEF), Cordoba, Spain 3Nutrir.. Nutrición Médica y Ejercicio, Cordoba, Spain 4Institute for Memory Impairments and Neurological Disorders (MIND Institute) University of California, Irvine 5Physical Education Department, School of Physical Activity and Sport Sciences, University of Las Palmas de Gran Canarias, Las Palmas, Spain

Article Title: Supplementation of arachidonic acid rich oil in European sea bass juveniles (Dicentrarchus labrax) diets: Effects on leucocytes and plasma fatty acid profiles, selected immune parameters and circulating prostaglandins levels.
Article Snippet: The main objective of this study was to assess the effects of graded levels of dietary arachidonic acid (ARA), supplemented from alternative sources, on fatty acid composition of plasma and head kidney leucocytes of European sea bass (Dicentrarchus labrax).. For that purpose, sea bass juveniles were fed four diets containing graded levels of ARA as follows: 0.5% (ARA0.5), 1% (ARA1), 2% (ARA2) and 4% (ARA4) during 60 days.. At the end of the feeding trial fatty acid profiles of plasma and head kidney leucocytes were analyzed.

Incubation:

Article Title: Supplementation of arachidonic acid rich oil in European sea bass juveniles (Dicentrarchus labrax) diets: Effects on leucocytes and plasma fatty acid profiles, selected immune parameters and circulating prostaglandins levels.
Article Snippet: The main objective of this study was to assess the effects of graded levels of dietary arachidonic acid (ARA), supplemented from alternative sources, on fatty acid composition of plasma and head kidney leucocytes of European sea bass (Dicentrarchus labrax).. For that purpose, sea bass juveniles were fed four diets containing graded levels of ARA as follows: 0.5% (ARA0.5), 1% (ARA1), 2% (ARA2) and 4% (ARA4) during 60 days.. At the end of the feeding trial fatty acid profiles of plasma and head kidney leucocytes were analyzed.

Bacteria:

Article Title: Supplementation of arachidonic acid rich oil in European sea bass juveniles (Dicentrarchus labrax) diets: Effects on leucocytes and plasma fatty acid profiles, selected immune parameters and circulating prostaglandins levels.
Article Snippet: The main objective of this study was to assess the effects of graded levels of dietary arachidonic acid (ARA), supplemented from alternative sources, on fatty acid composition of plasma and head kidney leucocytes of European sea bass (Dicentrarchus labrax).. For that purpose, sea bass juveniles were fed four diets containing graded levels of ARA as follows: 0.5% (ARA0.5), 1% (ARA1), 2% (ARA2) and 4% (ARA4) during 60 days.. At the end of the feeding trial fatty acid profiles of plasma and head kidney leucocytes were analyzed.



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Effect of IL-13Rα2 on migration and invasion activity of osteosarcoma cells: ( A ) Migration assay was conducted by seeding 1 × 10 5 cells into the upper chamber of each well. ( B ) Invasion assays were performed by seeding 2 × 10 5 cells in each well of the Matrigel-coated upper chamber. Serum-free media was used in the upper chamber for both migration and invasion assays, while the lower chamber contained media with 10% serum to serve as a chemoattractant. The migrated or invaded cells were <t>stained</t> with <t>Diff</t> <t>Quick</t> staining <t>solution</t> and counted in five separate ×100 microscopic fields per well. Original magnification, ×400. *** p < 0.001; EV, empty vector for wild-type IL-13Rα2; IL-13Rα2-OE, vector for wild-type IL-13Rα2; shControl, control vector for shRNA; shIL-13Rα2, vector for shRNA for IL-13Rα2.
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Effect of IL-13Rα2 on migration and invasion activity of osteosarcoma cells: ( A ) Migration assay was conducted by seeding 1 × 10 5 cells into the upper chamber of each well. ( B ) Invasion assays were performed by seeding 2 × 10 5 cells in each well of the Matrigel-coated upper chamber. Serum-free media was used in the upper chamber for both migration and invasion assays, while the lower chamber contained media with 10% serum to serve as a chemoattractant. The migrated or invaded cells were <t>stained</t> with <t>Diff</t> <t>Quick</t> staining <t>solution</t> and counted in five separate ×100 microscopic fields per well. Original magnification, ×400. *** p < 0.001; EV, empty vector for wild-type IL-13Rα2; IL-13Rα2-OE, vector for wild-type IL-13Rα2; shControl, control vector for shRNA; shIL-13Rα2, vector for shRNA for IL-13Rα2.
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Effect of IL-13Rα2 on migration and invasion activity of osteosarcoma cells: ( A ) Migration assay was conducted by seeding 1 × 10 5 cells into the upper chamber of each well. ( B ) Invasion assays were performed by seeding 2 × 10 5 cells in each well of the Matrigel-coated upper chamber. Serum-free media was used in the upper chamber for both migration and invasion assays, while the lower chamber contained media with 10% serum to serve as a chemoattractant. The migrated or invaded cells were stained with Diff Quick staining solution and counted in five separate ×100 microscopic fields per well. Original magnification, ×400. *** p < 0.001; EV, empty vector for wild-type IL-13Rα2; IL-13Rα2-OE, vector for wild-type IL-13Rα2; shControl, control vector for shRNA; shIL-13Rα2, vector for shRNA for IL-13Rα2.

Journal: Pharmaceuticals

Article Title: IL-13Rα2 Is Involved in Resistance to Doxorubicin and Survival of Osteosarcoma Patients

doi: 10.3390/ph17111526

Figure Lengend Snippet: Effect of IL-13Rα2 on migration and invasion activity of osteosarcoma cells: ( A ) Migration assay was conducted by seeding 1 × 10 5 cells into the upper chamber of each well. ( B ) Invasion assays were performed by seeding 2 × 10 5 cells in each well of the Matrigel-coated upper chamber. Serum-free media was used in the upper chamber for both migration and invasion assays, while the lower chamber contained media with 10% serum to serve as a chemoattractant. The migrated or invaded cells were stained with Diff Quick staining solution and counted in five separate ×100 microscopic fields per well. Original magnification, ×400. *** p < 0.001; EV, empty vector for wild-type IL-13Rα2; IL-13Rα2-OE, vector for wild-type IL-13Rα2; shControl, control vector for shRNA; shIL-13Rα2, vector for shRNA for IL-13Rα2.

Article Snippet: Cells that migrated or invaded to the lower side of the membrane were stained with Diff Quick stain solution (#468.037.1A, Diff-Quick, Dade Behring Inc., Newark, DE, USA).

Techniques: Migration, Activity Assay, Staining, Diff-Quik, Plasmid Preparation, Control, shRNA