giemsa staining diff quick fix (Medion Diagnostics)
90
Structured Review
Medion Diagnostics
giemsa staining diff quick fix
Giemsa Staining Diff Quick Fix, supplied by Medion Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diff+quick+fixative/modified+wright+giemsa+dye+diff+quik/pmc08017142-50-18-23
Average 90 stars, based on 1 article reviews
Giemsa Staining Diff Quick Fix, supplied by Medion Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diff+quick+fixative/modified+wright+giemsa+dye+diff+quik/pmc08017142-50-18-23
Average 90 stars, based on 1 article reviews
giemsa staining diff quick fix - by Bioz Stars,
2026-10
90/100 stars
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1) Product Images from "Enhanced Glycolysis Is Required for Antileishmanial Functions of Neutrophils Upon Infection With Leishmania donovani"
Article Title: Enhanced Glycolysis Is Required for Antileishmanial Functions of Neutrophils Upon Infection With Leishmania donovani
Journal: Frontiers in Immunology
doi: 10.3389/fimmu.2021.632512
Figure Legend Snippet: Glycolysis stress test profile of L. donovani -infected neutrophils. Primary human neutrophils were infected with L. donovani promastigotes (ratio 1:10) for 3 h at 37°C and 5% CO 2 . Uninfected cells served as control. The infection rate was determined by Giemsa staining of cytocentrifuged samples. Subsequently, free, non-ingested parasites were removed by washing. After 6 h post infection the glycolysis stress test profile was determined by using the Seahorse extracellular flux analyzer (A) . Extracellular acidification rate (ECAR) measurements following the sequential injection of 5 mM glucose, 1 μM oligomycin, and 10 mM 2-DG (dotted lines indicate injection time) were used to calculate key parameters of glycolytic function. Glycolysis (B) was calculated by subtraction of 2-DG-mediated ECAR from glucose-mediated ECAR. Glycolytic capacity (C) was calculated by subtraction of 2-DG-mediated ECAR from oligomycin-mediated ECAR. Bar graphs show mean ± SD ( n = 8), ** p ≤ 0.01.
Techniques Used: Infection, Control, Staining, Injection
Figure Legend Snippet: Mitochondrial stress test profile of L. donovani -infected neutrophils. Primary human neutrophils were infected with L. donovani promastigotes (ratio 1:10) for 3 h at 37°C and 5% CO 2 . Uninfected cells served as control. Successful infection was determined by Giemsa staining of cytocentrifuged samples. Subsequently, free, non-ingested parasites were removed by washing. After 6 h post infection the mitochondrial stress test profile was determined by using the Seahorse extracellular flux analyzer (A) . The measurement of basal oxygen consumption rate (OCR) was followed by sequential injections of 1 μM oligomycin, 1.5 μM FCCP, and 1 μM rotenone/antimycin A (dotted lines indicate injection time). OCR measurements were used to calculate key parameters of mitochondrial function. Non-mitochondrial respiration (B) was calculated as OCR after rotenone/antimycin A injection. Basal respiration (C) was calculated by subtraction of rotenone/antimycin A-mediated OCR from basal OCR. Maximal respiration (D) was calculated by subtraction of rotenone/antimycin A-mediated OCR from FCCP-mediated OCR. Proton leak (E) was calculated by subtraction of non-mitochondrial respiration from oligomycin-mediated OCR. ATP production (F) was calculated by subtraction of oligomycin-mediated OCR from basal OCR. Bar graphs show mean ± SD ( n = 3), ** p ≤ 0.01, ns, not significant.
Techniques Used: Infection, Control, Staining, Injection
Figure Legend Snippet: 2-NBDG uptake of L. donovani -infected neutrophils. Primary human neutrophils were infected with L. donovani promastigotes (ratio 1:10) for 3 h at 37°C and 5 % CO 2 . Uninfected cells served as control. The infection rate was determined by Giemsa staining of cytocentrifuged samples. Subsequently, free, non-ingested parasites were removed by washing. After 6 h post infection the fluorescent glucose analog 2-NBDG was added to the cells in glucose-free medium for the last 10 min of incubation time and 2-NBDG uptake was analyzed by flow cytometry. The bar diagram shows the autofluorescence corrected mean fluorescence intensity (MFI) of 2-NBDG uptake ± SD ( n = 3), *** p ≤ 0.001.
Techniques Used: Infection, Control, Staining, Incubation, Flow Cytometry, Fluorescence
Figure Legend Snippet: Lactate secretion and pyruvate content of L. donovani -infected neutrophils. Primary human neutrophils were infected with L. donovani promastigotes (ratio 1:10) for 3 h at 37°C and 5% CO 2 . Uninfected cells served as control. The infection rate was determined by Giemsa staining of cytocentrifuged samples. Subsequently, free, non-ingested parasites were removed by washing. Whole cell lysates were generated after 6 h post infection by boiling cells at 90°C for 5 min. Cell-free supernatants were collected after 6 h post infection. Lactate was detected in culture supernatants by using a lactate assay kit. Pyruvate was detected in whole cell lysates by using a pyruvate assay kit. Bar diagrams show mean concentration of lactate (A) after subtraction of the medium blanks and pyruvate (B) calculated by interpolation from standard curve ± SD ( n = 5), * p ≤ 0.05, **** p ≤ 0.0001.
Techniques Used: Infection, Control, Staining, Generated, Lactate Assay, Pyruvate Assay, Concentration Assay
Figure Legend Snippet: ATP concentration in L. donovani -infected neutrophils as response to metabolic inhibitors. Primary human neutrophils were infected with L. donovani promastigotes (ratio 1:10) for 3 h at 37°C and 5% CO 2 . Uninfected cells served as control. The infection rate was determined by Giemsa staining of cytocentrifuged samples. Subsequently, free, non-ingested parasites were removed by washing. After 6 h post infection cells were treated with 10 mM 2-DG for 3 h at 37°C and 5% CO 2 . PBS served as solvent control. Whole cell lysates were prepared and the ATP concentration was determined by using the ATP determination kit. Bar diagrams show the mean ATP concentration ± SD ( n = 3) in uninfected (A) and L. donovani -infected neutrophils (B) , * p ≤ 0.05, *** p ≤ 0.001, **** p ≤ 0.0001.
Techniques Used: Concentration Assay, Infection, Control, Staining, Solvent
Figure Legend Snippet: Survival of L. donovani promastigotes in 2-DG-treated neutrophils. Primary human neutrophils were infected with L. donovani promastigotes (ratio 1:10) for 3 h at 37°C, 5% CO 2 . The infection rate was determined by Giemsa staining of cytocentrifuged samples. After removing the free, non-ingested parasites the infected cells were treated with PBS, 5 mM, 50 mM, or 100 mM 2-DG. Survival of parasites was assessed after 24 h post infection by using the limiting dilution assay. The bar diagram shows the mean survival rates (%) normalized to PBS-treated control cells ± SD ( n = 3), **** p ≤ 0.0001.
Techniques Used: Infection, Staining, Limiting Dilution Assay, Control
Figure Legend Snippet: ROS production of 2-DG-treated L. donovani -infected neutrophils. Primary human neutrophils were infected with L. donovani promastigotes (ratio 1:10) for 3 h at 37°C, 5% CO 2 . The infection rate was determined by Giemsa staining of cytocentrifuged samples. After removing the free, non-ingested parasites the infected and uninfected cells were treated with PBS, 5 mM, 50 mM, or 100 mM 2-DG. After 24 h post infection the MPO-derived ROS production was measured for 1 h at 37°C and 5% CO 2 after the stimulation with 20 nM PMA by using the luminol-based chemiluminescence assay. A representative curve of luminol chemiluminescence is shown in panel (A) . The bar diagram (B) shows the mean area under the curve (AUC) values ± SD ( n = 3), * p ≤ 0.05.
Techniques Used: Infection, Staining, Derivative Assay, Chemiluminescence Immunoassay
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