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diamino‑benzidine  (OriGene)


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    Structured Review

    OriGene diamino‑benzidine
    Diamino‑Benzidine, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/diamino+benzidine+dab+kit/Polink-2+HRP+Broad-2+(Ms%2E+%26+Rb%2E)+with+DAB+Kit/pm38495835-69-44-48
    Average 93 stars, based on 36 article reviews
    diamino‑benzidine - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Functional Segregation of Pancreatic Microcirculation Reveals Sex‐Dependent Microhemodynamic Signatures
    Article Snippet: Sections were subsequently incubated overnight at 4°C with primary antibodies targeting CD31 (1:25; Santa Cruz, sc‐101454), estrogen receptor alpha (ER‐α, 1:25; Abcam, ab32063), and estrogen receptor beta (ER‐β, 1:25; Abcam, ab187291). .. After incubation with appropriate secondary antibodies, immunoreactivity was visualized using a 3,3′‐diaminobenzidine (DAB) substrate kit (Zhongshan Golden Bridge) and sections were counterstained with hematoxylin. .. Microvascular density (MVD) was assessed by manual enumeration of CD31‐positive microvessels (six random ×40 fields), whereas ER‐α and ER‐β expression was quantified by measuring the optical density (OD) of the DAB signal using ImageJ software (National Institutes of Health, Bethesda, MD, USA) with the Fiji color deconvolution plugin (Fiji team, https://fiji.sc/ ).

    Article Title: Placental mitochondrial DNA mutation-related oxidative stress underlies subsequent risk of adverse prognosis in sFGR twins.
    Article Snippet: .. Samples were incubated with primary antibodies against 8-hydroxy-2'-deoxyguanosine (8-OHdG) (1:200 dilution, ab48508, Abcam) and cleaved caspase 3 (1:2000 dilution, 9664S, Cell Signaling Technology), and then incubated with PV-6000 immunohistochemistry kit (PV6000, Beijing Zhongshan Golden Bridge Biotechnology) sequentially. ..

    Immunofluorescence:

    Article Title: Sinisan, a compound Chinese herbal medicine, alleviates acute colitis by facilitating colonic secretory cell lineage commitment and mucin production
    Article Snippet: Objective: Ulcerative colitis is closely associated with intestinal stem cell (ISC) loss and impaired intestinal mucus barrier.. Sinisan (SNS), a compound Chinese herbal medicine, has a long history in the treatment of intestinal dysfunction, yet whether SNS can relieve acute experimental colitis by modulating ISC proliferation and secretory cell differentiation has not been studied.. Our study tested the effect of SNS against acute colitis and focused on the mechanisms involving intestinal barrier recovery.

    Staining:

    Article Title: Sinisan, a compound Chinese herbal medicine, alleviates acute colitis by facilitating colonic secretory cell lineage commitment and mucin production
    Article Snippet: Objective: Ulcerative colitis is closely associated with intestinal stem cell (ISC) loss and impaired intestinal mucus barrier.. Sinisan (SNS), a compound Chinese herbal medicine, has a long history in the treatment of intestinal dysfunction, yet whether SNS can relieve acute experimental colitis by modulating ISC proliferation and secretory cell differentiation has not been studied.. Our study tested the effect of SNS against acute colitis and focused on the mechanisms involving intestinal barrier recovery.

    Article Title: Role of ILC2s as Potential Effector Cells of IL25-Mediated Type 2 Inflammation in Chronic Rhinosinusitis with Nasal Polyps in China
    Article Snippet: .. Immunohistochemical (IHC) staining was performed using the Polink-2 HRP Plus Broad DAB Detection System (Golden Bridge International Labs, Bothell, WA). ..

    Immunohistochemistry:

    Article Title: Sinisan, a compound Chinese herbal medicine, alleviates acute colitis by facilitating colonic secretory cell lineage commitment and mucin production
    Article Snippet: Objective: Ulcerative colitis is closely associated with intestinal stem cell (ISC) loss and impaired intestinal mucus barrier.. Sinisan (SNS), a compound Chinese herbal medicine, has a long history in the treatment of intestinal dysfunction, yet whether SNS can relieve acute experimental colitis by modulating ISC proliferation and secretory cell differentiation has not been studied.. Our study tested the effect of SNS against acute colitis and focused on the mechanisms involving intestinal barrier recovery.

    Article Title: Role of ILC2s as Potential Effector Cells of IL25-Mediated Type 2 Inflammation in Chronic Rhinosinusitis with Nasal Polyps in China
    Article Snippet: .. Immunohistochemical (IHC) staining was performed using the Polink-2 HRP Plus Broad DAB Detection System (Golden Bridge International Labs, Bothell, WA). ..

    Article Title: Placental mitochondrial DNA mutation-related oxidative stress underlies subsequent risk of adverse prognosis in sFGR twins.
    Article Snippet: .. Samples were incubated with primary antibodies against 8-hydroxy-2'-deoxyguanosine (8-OHdG) (1:200 dilution, ab48508, Abcam) and cleaved caspase 3 (1:2000 dilution, 9664S, Cell Signaling Technology), and then incubated with PV-6000 immunohistochemistry kit (PV6000, Beijing Zhongshan Golden Bridge Biotechnology) sequentially. ..

    Article Title: Pitavastatin reduces intestinal fibrosis in chronic colitis and inhibits colon fibroblast activation by enhancing MMP-9 expression via the IGF-1/IGF-1R pathway
    Article Snippet: .. IHC was conducted according to the instructions of the Universal Two-step Detection kit (Zhongshan Golden Bridge, China). .. Colon sections were incubated with primary antibody overnight at 4°C, and HRP-conjugated anti-rabbit antibody was incubated at room temperature for 40 min and the blots were then developed with the DAB kit (Zhongshan Golden Bridge).

    Immunohistochemical staining:

    Article Title: Role of ILC2s as Potential Effector Cells of IL25-Mediated Type 2 Inflammation in Chronic Rhinosinusitis with Nasal Polyps in China
    Article Snippet: .. Immunohistochemical (IHC) staining was performed using the Polink-2 HRP Plus Broad DAB Detection System (Golden Bridge International Labs, Bothell, WA). ..



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