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dhplc transgenomic wave nucleic acid fragment analysis system  (Transgenomic)

 
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    Structured Review

    Transgenomic dhplc transgenomic wave nucleic acid fragment analysis system
    Dhplc Transgenomic Wave Nucleic Acid Fragment Analysis System, supplied by Transgenomic, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dhplc+analysis/wave+dhplc+system/pmc07967950-159-6-5
    Average 90 stars, based on 1 article reviews
    dhplc transgenomic wave nucleic acid fragment analysis system - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Adenomatous Polyposis Coli Gene Mutations in 22 Chinese Pedigrees with Familial Adenomatous Polyposis
    Article Snippet: .. For micromutations, DHPLC was carried out after PCR of the extracted DNA from the blood sample to monitor the PCR product with specific abnormal elution profile (waveform) using the WAVE DHPLC system (Transgenomic, Omaha, NE, USA), with a single injection volume of 5-8 μL, column temperature of 55–62°C, mobile phase of 0.1 mM N-triethyl acetamide and different concentrations of ethyl cyanide, flow velocity of 0.9 ml/min, and detection wavelength of 260 nm. .. The PCR product with specific abnormal waveform was then sequenced using an Illumina Hiseq2500 analyzer (Illumina, San Diego, CA, USA).

    Article Title: Comparison of clinical characteristics of a pediatric cohort with combined pituitary hormone deficiency caused by mutation of the PROP1 gene or of other origins.
    Article Snippet: .. Genetic analysis of the PROP1 gene was performed using PCR amplification of all three exons of the PROP1 gene; subsequent screening analysis of the obtained amplicons was performed by means of DHPLC chromatography (Wave Transgenomic, USA) to detect presumed structural DNA variants. ..

    Article Title: Clinical implications of novel activating EGFR mutations in malignant peritoneal mesothelioma
    Article Snippet: .. Amplified PCR products were first screened by DHPLC heteroduplex analysis using the Transgenomic WAVE ® system (Transgenomic, Omaha, NE) as previously described. .. Samples with variant DHPLC profiles were purified with QIAquick spin columns and sequenced directly (BigDye ® Terminators sequencing kit, Foster City, CA) in both directions using an automated ABI 3100 genetic analysis system and analyzed using the Sequencer 4.1 software program package (Gene Codes ® , Ann Arbor, MI).

    Article Title: Differential expression of selected histone modifier genes in human solid cancers
    Article Snippet: .. PCR products of 8 samples were pooled and injected in the Transgenomics WAVE DHPLC using 3 different temperatures. ..

    Injection:

    Article Title: Adenomatous Polyposis Coli Gene Mutations in 22 Chinese Pedigrees with Familial Adenomatous Polyposis
    Article Snippet: .. For micromutations, DHPLC was carried out after PCR of the extracted DNA from the blood sample to monitor the PCR product with specific abnormal elution profile (waveform) using the WAVE DHPLC system (Transgenomic, Omaha, NE, USA), with a single injection volume of 5-8 μL, column temperature of 55–62°C, mobile phase of 0.1 mM N-triethyl acetamide and different concentrations of ethyl cyanide, flow velocity of 0.9 ml/min, and detection wavelength of 260 nm. .. The PCR product with specific abnormal waveform was then sequenced using an Illumina Hiseq2500 analyzer (Illumina, San Diego, CA, USA).

    Article Title: Differential expression of selected histone modifier genes in human solid cancers
    Article Snippet: .. PCR products of 8 samples were pooled and injected in the Transgenomics WAVE DHPLC using 3 different temperatures. ..

    Amplification:

    Article Title: Comparison of clinical characteristics of a pediatric cohort with combined pituitary hormone deficiency caused by mutation of the PROP1 gene or of other origins.
    Article Snippet: .. Genetic analysis of the PROP1 gene was performed using PCR amplification of all three exons of the PROP1 gene; subsequent screening analysis of the obtained amplicons was performed by means of DHPLC chromatography (Wave Transgenomic, USA) to detect presumed structural DNA variants. ..

    Article Title: Clinical implications of novel activating EGFR mutations in malignant peritoneal mesothelioma
    Article Snippet: .. Amplified PCR products were first screened by DHPLC heteroduplex analysis using the Transgenomic WAVE ® system (Transgenomic, Omaha, NE) as previously described. .. Samples with variant DHPLC profiles were purified with QIAquick spin columns and sequenced directly (BigDye ® Terminators sequencing kit, Foster City, CA) in both directions using an automated ABI 3100 genetic analysis system and analyzed using the Sequencer 4.1 software program package (Gene Codes ® , Ann Arbor, MI).

    Chromatography:

    Article Title: Comparison of clinical characteristics of a pediatric cohort with combined pituitary hormone deficiency caused by mutation of the PROP1 gene or of other origins.
    Article Snippet: .. Genetic analysis of the PROP1 gene was performed using PCR amplification of all three exons of the PROP1 gene; subsequent screening analysis of the obtained amplicons was performed by means of DHPLC chromatography (Wave Transgenomic, USA) to detect presumed structural DNA variants. ..

    other:

    Article Title: Resequencing of genes for transforming growth factor β1 ( TGFB1 ) type 1 and 2 receptors ( TGFBR1 , TGFBR2 ), and association analysis of variants with diabetic nephropathy
    Article Snippet: Each PCR product was then evaluated using WaveMaker v3.4 software (Transgenomic Ltd, Crewe, UK) and analysed on the WAVETM (dHPLC) DNA Fragment Analysis System (Transgenomic Ltd) following the manufacturer's recommendations.



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