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difluoromethylornithine  (Tocris)


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    Structured Review

    Tocris difluoromethylornithine
    Difluoromethylornithine, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dfmo/DFMO/pm41086975-70-22-26
    Average 93 stars, based on 22 article reviews
    difluoromethylornithine - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    other:

    Article Title: Polyamine biosynthesis and eIF5A hypusination are modulated by the DNA tumor virus KSHV and promote KSHV viral infection
    Article Snippet: 2-difluoromethylornithine (DFMO) (Cat#2761), ribavirin (Cat#R0077), and deoxyhypusine synthase inhibitor N1-guanyl-1,7-diaminoheptane (GC7) (Cat#259545) were purchased from Tocris, Tokyo Chemical Industry, and EMD Millipore respectively.

    Cell Differentiation:

    Article Title: Polyamines from myeloid-derived suppressor cells promote Th17 polarization and disease progression
    Article Snippet: Cells collected from humans were cultured in medium containing 5 mg/mL anti-human CD3 mAb (OKT3, eBioscience), 1 mg/mL soluble anti-human CD28mAb (CD28.2, eBioscience), 50 mM2-mercaptoethanol (Sigma), 2.5 ng/mL human TGF-b (R&D), 20 ng/mL human IL-6 (R&D), 10 ng/mL human IL-23 (R&D), 10 ng/mL human IL-1b (R&D), 5 mg/mL anti-human IFN-g mAb (285-IF-100, R&D), and 5 mg/mL anti-mouse IL-4 mAb (MP4-25D2, BioXcell) for 6–7 days. .. To determine the role of MDSCs and polyamines in Th17 cell differentiation, MDSCs (at a 1:1 ratio to naive CD4+ T cells) were added in the presence or absence of ornithine (ORN, 100 nM, Sigma), difluoromethylornithine (DFMO, 20 nM, Tocris), putrescine (PUT, 2.5 mM, Sigma), spermidine (SPD, 2.5 mM, Sigma), or spermine (SPM, 2.5 mM, Sigma). ..

    Recombinant:

    Article Title: Transcriptional-translational conflict is a barrier to cellular transformation and cancer progression.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins N-Butyl-N-(4-hydroxybutyl)nitrosamine (BBN) ThermoFisher B0938 4-(N-Butyl-N-nitrosamino) butyric Acid (BCPN) TCI B3185 Homoharringtonine (HHT) Carbosynth FH15974 Puromycin Dihydrochloride ThermoFisher BP2956100 eEF2K inhibitor (A-484954) Sigma 324516 GSK126 Selleckchem.com S7061 DFMO Tocris Bioscience 2761 Anti-FGFR3 Recombinant Antibody clone PRO-001 Creative bio labs HPAB-0160-YC Tamoxifen Sigma T5648 Hydrocortisone Sigma H4001 Collagenase type II Life Technologies 17101015 Cell recovery solution Corning 354253 B-27 Supplement Gibco 17504–044 Murine EGF Peprotech 315–09 A83-01 Tocris 2939 Y-27632 dihydrochloride Sigma Y0503 Doxycycline ThermoFisher AC446060050 Proteinase K Sigma 3115879001 Mouse on Mouse (M.O.M.) .. Blocking Reagent Vector Laboratories MKB-2213 Pro-Long Gold Antifade mountant with DAPI ThermoFisher P36935 DAB solution Agilent K3467 Faramount mounting medium, Aqueous Agilent S302580 Histo Gel Epredia HG-4000-012 Cycloheximide Sigma C7698 Heparin sodium salt from porcine intestinal mucosa Sigma H3149 BioMag Plus Concanavalin A Bangs laboratories, Inc. BP531 Whatman glass microfiber filters, Grade GF/C Sigma WHA1822024 LLC Liquid scintillation cocktail ThermoFisher 50-904-0129 S35 EasyTagTM EXPRESS35S Protein Labeling Mix Perkin Elmer NEG772002MC Critical commercial assays CellTiter-Glo 2.0 Cell Viability Assay Promega G9242 Comet assay single cell gel electrophoresis assay kit Trevigen 4250-050-K Direct-zol Miniprep Plus kits Zymo Research ZR2070 TruSeq Stranded mRNA Library Prep Kit Illumina 20020594 TruSeq RNA UD Indexes kit Illumina 20022371 NovaSeq 6000 SP Reagent Kit Illumina 20027464 Trans-Blot Turbo RTA Midi 0.2 mm PVDF Transfer Kit Bio-Rad 1704273 Super Signal West Pico Chemiluminescent Substrate kit ThermoFisher PI34080 TMTpro18plex labeling reagent ThermoFisher A44521 and A52048 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Technology 9003S Propidium Iodide Flow Cytometry Kit Abcam ab139418 GoTaq Green Master Mix ThermoFisher PRM7123 ERCC RNA Spike-In Mix Invitrogen 4456740 (Continued on next page) Cancer Cell 41, 853–870.e1–e13, May 8, 2023 e3

    Cell Recovery:

    Article Title: Transcriptional-translational conflict is a barrier to cellular transformation and cancer progression.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins N-Butyl-N-(4-hydroxybutyl)nitrosamine (BBN) ThermoFisher B0938 4-(N-Butyl-N-nitrosamino) butyric Acid (BCPN) TCI B3185 Homoharringtonine (HHT) Carbosynth FH15974 Puromycin Dihydrochloride ThermoFisher BP2956100 eEF2K inhibitor (A-484954) Sigma 324516 GSK126 Selleckchem.com S7061 DFMO Tocris Bioscience 2761 Anti-FGFR3 Recombinant Antibody clone PRO-001 Creative bio labs HPAB-0160-YC Tamoxifen Sigma T5648 Hydrocortisone Sigma H4001 Collagenase type II Life Technologies 17101015 Cell recovery solution Corning 354253 B-27 Supplement Gibco 17504–044 Murine EGF Peprotech 315–09 A83-01 Tocris 2939 Y-27632 dihydrochloride Sigma Y0503 Doxycycline ThermoFisher AC446060050 Proteinase K Sigma 3115879001 Mouse on Mouse (M.O.M.) .. Blocking Reagent Vector Laboratories MKB-2213 Pro-Long Gold Antifade mountant with DAPI ThermoFisher P36935 DAB solution Agilent K3467 Faramount mounting medium, Aqueous Agilent S302580 Histo Gel Epredia HG-4000-012 Cycloheximide Sigma C7698 Heparin sodium salt from porcine intestinal mucosa Sigma H3149 BioMag Plus Concanavalin A Bangs laboratories, Inc. BP531 Whatman glass microfiber filters, Grade GF/C Sigma WHA1822024 LLC Liquid scintillation cocktail ThermoFisher 50-904-0129 S35 EasyTagTM EXPRESS35S Protein Labeling Mix Perkin Elmer NEG772002MC Critical commercial assays CellTiter-Glo 2.0 Cell Viability Assay Promega G9242 Comet assay single cell gel electrophoresis assay kit Trevigen 4250-050-K Direct-zol Miniprep Plus kits Zymo Research ZR2070 TruSeq Stranded mRNA Library Prep Kit Illumina 20020594 TruSeq RNA UD Indexes kit Illumina 20022371 NovaSeq 6000 SP Reagent Kit Illumina 20027464 Trans-Blot Turbo RTA Midi 0.2 mm PVDF Transfer Kit Bio-Rad 1704273 Super Signal West Pico Chemiluminescent Substrate kit ThermoFisher PI34080 TMTpro18plex labeling reagent ThermoFisher A44521 and A52048 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Technology 9003S Propidium Iodide Flow Cytometry Kit Abcam ab139418 GoTaq Green Master Mix ThermoFisher PRM7123 ERCC RNA Spike-In Mix Invitrogen 4456740 (Continued on next page) Cancer Cell 41, 853–870.e1–e13, May 8, 2023 e3



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    Image Search Results


    (A) SK-N-BE(2)-c cells were treated with five doses of QW-5–70 (0.125 to 2 nM) in combination with five doses of DFMO (10–1000 μM). Cell viability was assessed using the MTS assay (n=4). (B) CI–Fa analysis of the QW-5–70 and DFMO combination. CI values were calculated using the Chou–Talalay median-effect method for five experimentally tested dose pairs at their corresponding Fa values. CI < 1, CI = 1, and CI > 1 indicate synergistic, additive, and antagonistic interactions, respectively. (C) SK-N-BE(2)-c cells were treated with five doses of QW-5–70 (0.125 to 2 nM) in combination with five doses of MLN8237 (1–100 nM). Cell viability was assessed using the MTS assay (n=4). (D) SK-N-BE(2)-C cells were treated with 1 nM QW-5–70 in combination with either DFMO (300 μM, top) or MLN8237 (30 nM, bottom) as indicated. Cell lysates were analyzed by immunoblotting for cPARP as a marker of apoptosis, with GAPDH used as a loading control. (E) Representative colony formation images of SK-N-BE(2)-C cells treated with the same combination. Colony formations were quantified and expressed as mean ± SEM relative to vehicle (set to 100%) (n=5).

    Journal: Molecular cancer therapeutics

    Article Title: QW-5-70 targets the colchicine site and demonstrates antitumor activity in P-gp–overexpressing cancer models

    doi: 10.1158/1535-7163.MCT-25-1013

    Figure Lengend Snippet: (A) SK-N-BE(2)-c cells were treated with five doses of QW-5–70 (0.125 to 2 nM) in combination with five doses of DFMO (10–1000 μM). Cell viability was assessed using the MTS assay (n=4). (B) CI–Fa analysis of the QW-5–70 and DFMO combination. CI values were calculated using the Chou–Talalay median-effect method for five experimentally tested dose pairs at their corresponding Fa values. CI < 1, CI = 1, and CI > 1 indicate synergistic, additive, and antagonistic interactions, respectively. (C) SK-N-BE(2)-c cells were treated with five doses of QW-5–70 (0.125 to 2 nM) in combination with five doses of MLN8237 (1–100 nM). Cell viability was assessed using the MTS assay (n=4). (D) SK-N-BE(2)-C cells were treated with 1 nM QW-5–70 in combination with either DFMO (300 μM, top) or MLN8237 (30 nM, bottom) as indicated. Cell lysates were analyzed by immunoblotting for cPARP as a marker of apoptosis, with GAPDH used as a loading control. (E) Representative colony formation images of SK-N-BE(2)-C cells treated with the same combination. Colony formations were quantified and expressed as mean ± SEM relative to vehicle (set to 100%) (n=5).

    Article Snippet: Paclitaxel was purchased from LC Laboratories; colchicine and verapamil from Sigma; and Q-VD-OPh (QVD), tariquidar, DFMO (eflornithine), and MLN8237 from MedChemExpress.

    Techniques: Activity Assay, MTS Assay, Western Blot, Marker, Control