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dfmo  (Tocris)


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    Structured Review

    Tocris dfmo
    Dfmo, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dfmo/DFMO/pm38175754-262-8-11
    Average 93 stars, based on 21 article reviews
    dfmo - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    other:

    Article Title: The polyamine putrescine is a positive regulator of group 3 innate lymphocyte (ILC3) activation
    Article Snippet: Cell line MNK-3 cells clone B3 cells, derived from single cell cloning of the ILC3-like cell line MNK-3 ( 16 ), were maintained in DMEM (Corning; Tewksbury, MA) with 10% heat-inactivated FBS (Gemini Bio-Products; West Sacramento,, CA), 2 mM GlutaMax (Life Technologies; Carlsbad, CA), 1 mM sodium pyruvate (GE Healthcare Hyclone; Logan, UT), 55 μM β-mercaptoethanol (Sigma; St. Louis, MO), 10 mM HEPES (Corning), 50 μg/ml gentamycin (Amresco; Solon, OH), 100 U/ml penicillin (Gemini Bio-Products), 100 U/ml streptomycin (Gemini Bio-Products) and 10 ng/ml recombinant mouse IL-7 (Peprotech; Rocky Hill, NJ).

    Ex Vivo:

    Article Title: VISTA promotes the metabolism and differentiation of myeloid-derived suppressor cells by STAT3 and polyamine-dependent mechanisms.
    Article Snippet: .. For ex vivo treatment with inhibitors or chemicals, DFMO (400 mM) (Tocris Bioscience, Fisher Scientific, Cat no. 27-611-0), or putrescine (800 mM) (Fisher Scientific, Cat no. ICN10044105), or STAT3 inhibitor (2 mM), or CK2 inhibitor (20 mM), or combinations when indicated, were added to BM cell culture on day 2. ..

    Article Title: VISTA promotes the metabolism and differentiation of myeloid-derived suppressor cells by STAT3 and polyamine-dependent mechanisms
    Article Snippet: M-MDSCs were purified after depleting DCs, macrophages, and neutrophils/PMN-MDSCs, using a biotinylated antibody cocktail specific for CD11C, F4/80, and Ly6G and streptavidin-conjugated beads (EasySep Mouse Streptavidin RapidSpheres Isolation Kit, Stemcell Tech, Cat# 19860). .. For ex vivo treatment with inhibitors or chemicals, DFMO (400 μM) (Tocris Bioscience, Fisher Scientific, Cat no. 27-611-0), or putrescine (800 μM) (Fisher Scientific, Cat no. ICN10044105), or STAT3 inhibitor (2 μM), or CK2 inhibitor (20 μM), or combinations when indicated, were added to BM cell culture on day 2. ..

    Cell Culture:

    Article Title: VISTA promotes the metabolism and differentiation of myeloid-derived suppressor cells by STAT3 and polyamine-dependent mechanisms.
    Article Snippet: .. For ex vivo treatment with inhibitors or chemicals, DFMO (400 mM) (Tocris Bioscience, Fisher Scientific, Cat no. 27-611-0), or putrescine (800 mM) (Fisher Scientific, Cat no. ICN10044105), or STAT3 inhibitor (2 mM), or CK2 inhibitor (20 mM), or combinations when indicated, were added to BM cell culture on day 2. ..

    Article Title: VISTA promotes the metabolism and differentiation of myeloid-derived suppressor cells by STAT3 and polyamine-dependent mechanisms
    Article Snippet: M-MDSCs were purified after depleting DCs, macrophages, and neutrophils/PMN-MDSCs, using a biotinylated antibody cocktail specific for CD11C, F4/80, and Ly6G and streptavidin-conjugated beads (EasySep Mouse Streptavidin RapidSpheres Isolation Kit, Stemcell Tech, Cat# 19860). .. For ex vivo treatment with inhibitors or chemicals, DFMO (400 μM) (Tocris Bioscience, Fisher Scientific, Cat no. 27-611-0), or putrescine (800 μM) (Fisher Scientific, Cat no. ICN10044105), or STAT3 inhibitor (2 μM), or CK2 inhibitor (20 μM), or combinations when indicated, were added to BM cell culture on day 2. ..

    Derivative Assay:

    Article Title: Polyamines and hypusination are important for Clostridioides difficile toxin B (TcdB)-mediated activation of group 3 innate lymphocytes (ILC3s)
    Article Snippet: .. Cell line MNK-3 cell clone B3 cells, derived from single cell cloning of the ILC3-like cell line MNK-3 ( 20 ), were maintained in Dulbecco’s modified Eagle medium (DMEM) (Corning, Tewksbury, MA) with 10% heat-inactivated fetal bovine serum (FBS) (Gemini Bio-Products, West Sacramento, CA), 2 mM GlutaMax (Life Technologies, Carlsbad, CA), 1 mM sodium pyruvate (GE Healthcare HyClone, Logan, UT), 55 μM β-mercaptoethanol (Sigma, St. Louis, MO), 10 mM HEPES (Corning), 50 μg/mL gentamycin (Amresco, Solon, OH), 100 U/mL penicillin (Gemini Bio-Products), 100 U/mL streptomycin (Gemini Bio-Products), and 10 ng/mL recombinant mouse interleukin-7 (IL-7) (Peprotech, Rocky Hill, NJ). . ILC3 activation and treatment Cells were grown in the presence of 500 μM DFMO (Tocris Biosciences, Bristol, UK) for 3 days to deplete polyamines as described ( 18 ). .. For polyamine rescue experiments, 500 μM putrescine or 500 nM sperimidine (both from Sigma) was added with DFMO.

    Single Cell:

    Article Title: Polyamines and hypusination are important for Clostridioides difficile toxin B (TcdB)-mediated activation of group 3 innate lymphocytes (ILC3s)
    Article Snippet: .. Cell line MNK-3 cell clone B3 cells, derived from single cell cloning of the ILC3-like cell line MNK-3 ( 20 ), were maintained in Dulbecco’s modified Eagle medium (DMEM) (Corning, Tewksbury, MA) with 10% heat-inactivated fetal bovine serum (FBS) (Gemini Bio-Products, West Sacramento, CA), 2 mM GlutaMax (Life Technologies, Carlsbad, CA), 1 mM sodium pyruvate (GE Healthcare HyClone, Logan, UT), 55 μM β-mercaptoethanol (Sigma, St. Louis, MO), 10 mM HEPES (Corning), 50 μg/mL gentamycin (Amresco, Solon, OH), 100 U/mL penicillin (Gemini Bio-Products), 100 U/mL streptomycin (Gemini Bio-Products), and 10 ng/mL recombinant mouse interleukin-7 (IL-7) (Peprotech, Rocky Hill, NJ). . ILC3 activation and treatment Cells were grown in the presence of 500 μM DFMO (Tocris Biosciences, Bristol, UK) for 3 days to deplete polyamines as described ( 18 ). .. For polyamine rescue experiments, 500 μM putrescine or 500 nM sperimidine (both from Sigma) was added with DFMO.

    Cloning:

    Article Title: Polyamines and hypusination are important for Clostridioides difficile toxin B (TcdB)-mediated activation of group 3 innate lymphocytes (ILC3s)
    Article Snippet: .. Cell line MNK-3 cell clone B3 cells, derived from single cell cloning of the ILC3-like cell line MNK-3 ( 20 ), were maintained in Dulbecco’s modified Eagle medium (DMEM) (Corning, Tewksbury, MA) with 10% heat-inactivated fetal bovine serum (FBS) (Gemini Bio-Products, West Sacramento, CA), 2 mM GlutaMax (Life Technologies, Carlsbad, CA), 1 mM sodium pyruvate (GE Healthcare HyClone, Logan, UT), 55 μM β-mercaptoethanol (Sigma, St. Louis, MO), 10 mM HEPES (Corning), 50 μg/mL gentamycin (Amresco, Solon, OH), 100 U/mL penicillin (Gemini Bio-Products), 100 U/mL streptomycin (Gemini Bio-Products), and 10 ng/mL recombinant mouse interleukin-7 (IL-7) (Peprotech, Rocky Hill, NJ). . ILC3 activation and treatment Cells were grown in the presence of 500 μM DFMO (Tocris Biosciences, Bristol, UK) for 3 days to deplete polyamines as described ( 18 ). .. For polyamine rescue experiments, 500 μM putrescine or 500 nM sperimidine (both from Sigma) was added with DFMO.

    Modification:

    Article Title: Polyamines and hypusination are important for Clostridioides difficile toxin B (TcdB)-mediated activation of group 3 innate lymphocytes (ILC3s)
    Article Snippet: .. Cell line MNK-3 cell clone B3 cells, derived from single cell cloning of the ILC3-like cell line MNK-3 ( 20 ), were maintained in Dulbecco’s modified Eagle medium (DMEM) (Corning, Tewksbury, MA) with 10% heat-inactivated fetal bovine serum (FBS) (Gemini Bio-Products, West Sacramento, CA), 2 mM GlutaMax (Life Technologies, Carlsbad, CA), 1 mM sodium pyruvate (GE Healthcare HyClone, Logan, UT), 55 μM β-mercaptoethanol (Sigma, St. Louis, MO), 10 mM HEPES (Corning), 50 μg/mL gentamycin (Amresco, Solon, OH), 100 U/mL penicillin (Gemini Bio-Products), 100 U/mL streptomycin (Gemini Bio-Products), and 10 ng/mL recombinant mouse interleukin-7 (IL-7) (Peprotech, Rocky Hill, NJ). . ILC3 activation and treatment Cells were grown in the presence of 500 μM DFMO (Tocris Biosciences, Bristol, UK) for 3 days to deplete polyamines as described ( 18 ). .. For polyamine rescue experiments, 500 μM putrescine or 500 nM sperimidine (both from Sigma) was added with DFMO.

    Recombinant:

    Article Title: Polyamines and hypusination are important for Clostridioides difficile toxin B (TcdB)-mediated activation of group 3 innate lymphocytes (ILC3s)
    Article Snippet: .. Cell line MNK-3 cell clone B3 cells, derived from single cell cloning of the ILC3-like cell line MNK-3 ( 20 ), were maintained in Dulbecco’s modified Eagle medium (DMEM) (Corning, Tewksbury, MA) with 10% heat-inactivated fetal bovine serum (FBS) (Gemini Bio-Products, West Sacramento, CA), 2 mM GlutaMax (Life Technologies, Carlsbad, CA), 1 mM sodium pyruvate (GE Healthcare HyClone, Logan, UT), 55 μM β-mercaptoethanol (Sigma, St. Louis, MO), 10 mM HEPES (Corning), 50 μg/mL gentamycin (Amresco, Solon, OH), 100 U/mL penicillin (Gemini Bio-Products), 100 U/mL streptomycin (Gemini Bio-Products), and 10 ng/mL recombinant mouse interleukin-7 (IL-7) (Peprotech, Rocky Hill, NJ). . ILC3 activation and treatment Cells were grown in the presence of 500 μM DFMO (Tocris Biosciences, Bristol, UK) for 3 days to deplete polyamines as described ( 18 ). .. For polyamine rescue experiments, 500 μM putrescine or 500 nM sperimidine (both from Sigma) was added with DFMO.

    Activation Assay:

    Article Title: Polyamines and hypusination are important for Clostridioides difficile toxin B (TcdB)-mediated activation of group 3 innate lymphocytes (ILC3s)
    Article Snippet: .. Cell line MNK-3 cell clone B3 cells, derived from single cell cloning of the ILC3-like cell line MNK-3 ( 20 ), were maintained in Dulbecco’s modified Eagle medium (DMEM) (Corning, Tewksbury, MA) with 10% heat-inactivated fetal bovine serum (FBS) (Gemini Bio-Products, West Sacramento, CA), 2 mM GlutaMax (Life Technologies, Carlsbad, CA), 1 mM sodium pyruvate (GE Healthcare HyClone, Logan, UT), 55 μM β-mercaptoethanol (Sigma, St. Louis, MO), 10 mM HEPES (Corning), 50 μg/mL gentamycin (Amresco, Solon, OH), 100 U/mL penicillin (Gemini Bio-Products), 100 U/mL streptomycin (Gemini Bio-Products), and 10 ng/mL recombinant mouse interleukin-7 (IL-7) (Peprotech, Rocky Hill, NJ). . ILC3 activation and treatment Cells were grown in the presence of 500 μM DFMO (Tocris Biosciences, Bristol, UK) for 3 days to deplete polyamines as described ( 18 ). .. For polyamine rescue experiments, 500 μM putrescine or 500 nM sperimidine (both from Sigma) was added with DFMO.



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    Image Search Results


    (A) SK-N-BE(2)-c cells were treated with five doses of QW-5–70 (0.125 to 2 nM) in combination with five doses of DFMO (10–1000 μM). Cell viability was assessed using the MTS assay (n=4). (B) CI–Fa analysis of the QW-5–70 and DFMO combination. CI values were calculated using the Chou–Talalay median-effect method for five experimentally tested dose pairs at their corresponding Fa values. CI < 1, CI = 1, and CI > 1 indicate synergistic, additive, and antagonistic interactions, respectively. (C) SK-N-BE(2)-c cells were treated with five doses of QW-5–70 (0.125 to 2 nM) in combination with five doses of MLN8237 (1–100 nM). Cell viability was assessed using the MTS assay (n=4). (D) SK-N-BE(2)-C cells were treated with 1 nM QW-5–70 in combination with either DFMO (300 μM, top) or MLN8237 (30 nM, bottom) as indicated. Cell lysates were analyzed by immunoblotting for cPARP as a marker of apoptosis, with GAPDH used as a loading control. (E) Representative colony formation images of SK-N-BE(2)-C cells treated with the same combination. Colony formations were quantified and expressed as mean ± SEM relative to vehicle (set to 100%) (n=5).

    Journal: Molecular cancer therapeutics

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    doi: 10.1158/1535-7163.MCT-25-1013

    Figure Lengend Snippet: (A) SK-N-BE(2)-c cells were treated with five doses of QW-5–70 (0.125 to 2 nM) in combination with five doses of DFMO (10–1000 μM). Cell viability was assessed using the MTS assay (n=4). (B) CI–Fa analysis of the QW-5–70 and DFMO combination. CI values were calculated using the Chou–Talalay median-effect method for five experimentally tested dose pairs at their corresponding Fa values. CI < 1, CI = 1, and CI > 1 indicate synergistic, additive, and antagonistic interactions, respectively. (C) SK-N-BE(2)-c cells were treated with five doses of QW-5–70 (0.125 to 2 nM) in combination with five doses of MLN8237 (1–100 nM). Cell viability was assessed using the MTS assay (n=4). (D) SK-N-BE(2)-C cells were treated with 1 nM QW-5–70 in combination with either DFMO (300 μM, top) or MLN8237 (30 nM, bottom) as indicated. Cell lysates were analyzed by immunoblotting for cPARP as a marker of apoptosis, with GAPDH used as a loading control. (E) Representative colony formation images of SK-N-BE(2)-C cells treated with the same combination. Colony formations were quantified and expressed as mean ± SEM relative to vehicle (set to 100%) (n=5).

    Article Snippet: Paclitaxel was purchased from LC Laboratories; colchicine and verapamil from Sigma; and Q-VD-OPh (QVD), tariquidar, DFMO (eflornithine), and MLN8237 from MedChemExpress.

    Techniques: Activity Assay, MTS Assay, Western Blot, Marker, Control