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cell apoptosis detection kit  (Beyotime)


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    Structured Review

    Beyotime cell apoptosis detection kit
    10 wt% PCL/nHA enhances cell proliferation and reduces <t>apoptosis</t> via calcium ion regulation. (A) Morphological characteristics and viability of L929 cells cultured on PCL and PCL/nHA samples. (B) Statistical evaluation of L929 cells viability. (C) Morphological characteristics and viability of HSFs cultured on PCL and PCL/nHA samples. (D) Statistical evaluation of HSFs viability. (E) Time-dependent proliferation of L929 cells on PCL and PCL/nHA substrates over 1, 2, and 3 days. (F) Time-dependent proliferation of HSFs on PCL and PCL/nHA substrates over 1, 2, and 3 days. (G) Influence of varying nHA concentrations in PCL on L929 cells apoptosis. (H) Quantitative apoptosis assessment across experimental groups based on data from G. (I) Apoptosis of L929 cells cultured in media with varying calcium ion concentrations. (J) Statistical analysis of the apoptosis data presented in I. (K) The morphological characteristics and viability of L929 cells cultured in the CaCl 2 group, the nHA group (with calcium ion concentration equivalent to that in the CaCl 2 group), and the control group. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.
    Cell Apoptosis Detection Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/detection+kit/Apoptosis+Inducers+Kit/pmc13084373-53-4-20
    Average 99 stars, based on 143 article reviews
    cell apoptosis detection kit - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion"

    Article Title: Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2026.03.037

    10 wt% PCL/nHA enhances cell proliferation and reduces apoptosis via calcium ion regulation. (A) Morphological characteristics and viability of L929 cells cultured on PCL and PCL/nHA samples. (B) Statistical evaluation of L929 cells viability. (C) Morphological characteristics and viability of HSFs cultured on PCL and PCL/nHA samples. (D) Statistical evaluation of HSFs viability. (E) Time-dependent proliferation of L929 cells on PCL and PCL/nHA substrates over 1, 2, and 3 days. (F) Time-dependent proliferation of HSFs on PCL and PCL/nHA substrates over 1, 2, and 3 days. (G) Influence of varying nHA concentrations in PCL on L929 cells apoptosis. (H) Quantitative apoptosis assessment across experimental groups based on data from G. (I) Apoptosis of L929 cells cultured in media with varying calcium ion concentrations. (J) Statistical analysis of the apoptosis data presented in I. (K) The morphological characteristics and viability of L929 cells cultured in the CaCl 2 group, the nHA group (with calcium ion concentration equivalent to that in the CaCl 2 group), and the control group. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.
    Figure Legend Snippet: 10 wt% PCL/nHA enhances cell proliferation and reduces apoptosis via calcium ion regulation. (A) Morphological characteristics and viability of L929 cells cultured on PCL and PCL/nHA samples. (B) Statistical evaluation of L929 cells viability. (C) Morphological characteristics and viability of HSFs cultured on PCL and PCL/nHA samples. (D) Statistical evaluation of HSFs viability. (E) Time-dependent proliferation of L929 cells on PCL and PCL/nHA substrates over 1, 2, and 3 days. (F) Time-dependent proliferation of HSFs on PCL and PCL/nHA substrates over 1, 2, and 3 days. (G) Influence of varying nHA concentrations in PCL on L929 cells apoptosis. (H) Quantitative apoptosis assessment across experimental groups based on data from G. (I) Apoptosis of L929 cells cultured in media with varying calcium ion concentrations. (J) Statistical analysis of the apoptosis data presented in I. (K) The morphological characteristics and viability of L929 cells cultured in the CaCl 2 group, the nHA group (with calcium ion concentration equivalent to that in the CaCl 2 group), and the control group. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.

    Techniques Used: Cell Culture, Concentration Assay, Control

    Piezo1-mediated Ca 2+ signaling in PCL/nHA-Driven cell behaviors. (A) Fluorescence imaging of intracellular calcium ion influx in L929 cells on PCL and PCL/nHA surfaces. (B) Fluorescence quantification of calcium ion influx in A. (C) Fluorescence imaging of intracellular calcium ion influx in HSFs on PCL and PCL/nHA surfaces. (D) Fluorescence quantification of calcium ion influx in C. (E) Fluorescence imaging assessment of intracellular ROS levels in L929 cells on PCL and PCL/nHA samples. (F) Effects of PCL samples with different nHA contents on the mitochondrial membrane potential of L929 cells. (G) Assessment of the expression levels of mechanosensing- and proliferation-related proteins in L929 cells cultured on PCL and PCL/nHA surfaces. (H) Assessment of the expression levels of mechanosensing- and proliferation-related proteins in HSFs cultured on PCL and PCL/nHA surfaces. (I) Assessment of the expression levels of apoptosis-related proteins in L929 cells cultured on PCL and PCL/nHA surfaces. (J) Schematic illustration depicting the mechanisms underlying L929 cell proliferation differences. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.
    Figure Legend Snippet: Piezo1-mediated Ca 2+ signaling in PCL/nHA-Driven cell behaviors. (A) Fluorescence imaging of intracellular calcium ion influx in L929 cells on PCL and PCL/nHA surfaces. (B) Fluorescence quantification of calcium ion influx in A. (C) Fluorescence imaging of intracellular calcium ion influx in HSFs on PCL and PCL/nHA surfaces. (D) Fluorescence quantification of calcium ion influx in C. (E) Fluorescence imaging assessment of intracellular ROS levels in L929 cells on PCL and PCL/nHA samples. (F) Effects of PCL samples with different nHA contents on the mitochondrial membrane potential of L929 cells. (G) Assessment of the expression levels of mechanosensing- and proliferation-related proteins in L929 cells cultured on PCL and PCL/nHA surfaces. (H) Assessment of the expression levels of mechanosensing- and proliferation-related proteins in HSFs cultured on PCL and PCL/nHA surfaces. (I) Assessment of the expression levels of apoptosis-related proteins in L929 cells cultured on PCL and PCL/nHA surfaces. (J) Schematic illustration depicting the mechanisms underlying L929 cell proliferation differences. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.

    Techniques Used: Fluorescence, Imaging, Membrane, Expressing, Cell Culture

    Related Articles

    Staining:

    Article Title: A photodynamically activated nanoplatform relieves glucose-driven immunosuppression to potentiate STING immunotherapy in triple-negative breast cancer
    Article Snippet: Pre-screened foetal bovine serum (FBP-C520) was procured from Haixing Biosciences. .. DAPI Staining Solution, Cell Viability/Cytotoxicity assay kit, Cell Cycle and Apoptosis analysis kit, Reactive Oxygen Species (ROS) assay kit, and Mitochondrial Membrane Potential assay kit with JC-1 were all purchased from Beyotime, Inc. .. Cell Counting Kit-8 was obtained from TargetMol, USA.

    Article Title: Triamcinolone acetonide nanoparticles ameliorate interstitial cystitis and anxiety/depression by targeting the bladder–metabolic–brain axis
    Article Snippet: .. After 12 h of LPS stimulation (1 μg/mL), cells were treated with free TA, blank CA-αCD NPs, TA/CA-αCD NPs, or TA/cRGD-CA-αCD NPs for another 24 h. Cells were then collected, resuspended in 500 μL PBS, and stained with 5 μl FITC-annexin V and 5 μl PI from the apoptosis detection kit obtained from Beyotime Biotechnology Co., Ltd. (Shanghai, China; Cat. No. C1062M). .. Apoptosis was assessed by flow cytometry (BD FACSAria III, USA).

    Article Title: Positioning creation of cell communication channels via Chinese microneedle
    Article Snippet: RNA isolator Total RNA Extraction Reagent was acquired from Vazyme Biotech Co., Ltd. (Jiangsu, China). .. Cell Cycle and Apoptosis Kit, Calcein/PI Cell Viability/Cytotoxicity Assay kit, Senescence β-Galactosidase Staining kit, Reactive Oxygen Species kit and DCFH-DA probe and JC-1 mitochondrial membrane potential detection kit were provided by Beyotime Biotechnology (Shanghai, China). .. Live and Dead Cell Staining kit was obtained from Abbkine Scientific Co., Ltd. (Wuhan, China).

    ROS Assay:

    Article Title: A photodynamically activated nanoplatform relieves glucose-driven immunosuppression to potentiate STING immunotherapy in triple-negative breast cancer
    Article Snippet: Pre-screened foetal bovine serum (FBP-C520) was procured from Haixing Biosciences. .. DAPI Staining Solution, Cell Viability/Cytotoxicity assay kit, Cell Cycle and Apoptosis analysis kit, Reactive Oxygen Species (ROS) assay kit, and Mitochondrial Membrane Potential assay kit with JC-1 were all purchased from Beyotime, Inc. .. Cell Counting Kit-8 was obtained from TargetMol, USA.

    Article Title: Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion
    Article Snippet: Cell culture medium, Penicillin-streptomycin (P/S), 0.25% trypsin solution, and Serum-free cell freezing medium were obtained from Procell Life Science & Technology Co. (China). .. CCK-8 assay kit, Fluo-4AM, Cell Apoptosis Detection Kit, Mitochondrial membrane potential assay kit and ROS Assay Kit were purchased from Beyotime Biotechnology (China). .. Bovine Primary antibodies targeting Piezo1, ITGB1, and Fibronectin were acquired from Cell Signaling Technology, Inc. (USA).

    Membrane:

    Article Title: A photodynamically activated nanoplatform relieves glucose-driven immunosuppression to potentiate STING immunotherapy in triple-negative breast cancer
    Article Snippet: Pre-screened foetal bovine serum (FBP-C520) was procured from Haixing Biosciences. .. DAPI Staining Solution, Cell Viability/Cytotoxicity assay kit, Cell Cycle and Apoptosis analysis kit, Reactive Oxygen Species (ROS) assay kit, and Mitochondrial Membrane Potential assay kit with JC-1 were all purchased from Beyotime, Inc. .. Cell Counting Kit-8 was obtained from TargetMol, USA.

    Article Title: Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion
    Article Snippet: Cell culture medium, Penicillin-streptomycin (P/S), 0.25% trypsin solution, and Serum-free cell freezing medium were obtained from Procell Life Science & Technology Co. (China). .. CCK-8 assay kit, Fluo-4AM, Cell Apoptosis Detection Kit, Mitochondrial membrane potential assay kit and ROS Assay Kit were purchased from Beyotime Biotechnology (China). .. Bovine Primary antibodies targeting Piezo1, ITGB1, and Fibronectin were acquired from Cell Signaling Technology, Inc. (USA).

    Article Title: Positioning creation of cell communication channels via Chinese microneedle
    Article Snippet: RNA isolator Total RNA Extraction Reagent was acquired from Vazyme Biotech Co., Ltd. (Jiangsu, China). .. Cell Cycle and Apoptosis Kit, Calcein/PI Cell Viability/Cytotoxicity Assay kit, Senescence β-Galactosidase Staining kit, Reactive Oxygen Species kit and DCFH-DA probe and JC-1 mitochondrial membrane potential detection kit were provided by Beyotime Biotechnology (Shanghai, China). .. Live and Dead Cell Staining kit was obtained from Abbkine Scientific Co., Ltd. (Wuhan, China).

    CCK-8 Assay:

    Article Title: Timosaponin AⅢ and Gefitinib Synergistically Inhibit NSCLC by Targeting RAGE/AKT/NF-κB/VEGF Pathway.
    Article Snippet: Introduction: EGFR tyrosine kinase inhibitors (EGFR-TKIs) are the standard therapy for EGFR-mutated advanced Non-Small Cell Lung Cancer (NSCLC).. Gefitinib (GEF), as a first-generation EGFR-TKI, has made significant progress in the treatment of advanced lung cancer.. However, acquired resistance leading to treatment failure severely limits its clinical benefits.

    Article Title: Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion
    Article Snippet: Cell culture medium, Penicillin-streptomycin (P/S), 0.25% trypsin solution, and Serum-free cell freezing medium were obtained from Procell Life Science & Technology Co. (China). .. CCK-8 assay kit, Fluo-4AM, Cell Apoptosis Detection Kit, Mitochondrial membrane potential assay kit and ROS Assay Kit were purchased from Beyotime Biotechnology (China). .. Bovine Primary antibodies targeting Piezo1, ITGB1, and Fibronectin were acquired from Cell Signaling Technology, Inc. (USA).



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