Cell Culture:Article Title: Method of producing pancreatic hormone-producing cells
Article Snippet: .. First, human iPS cells (iPS cells obtained by introducing Oct3/4gene, Klf4 gene and Sox2 gene: see Nat Biotechnol 2008; 26: 101-106) cultured and maintained in the state of a cell mass together with feeder cells were detached in the state of a cell mass by using a cell dissociation solution for primate ES cell (ReproCELL Inc.), these cells were transferred into a 15 ml centrifuge tube and stood for 5 min. ..
Article Title: Human pluripotent stem cells induced from undifferentiated stem cells derived from a human postnatal tissue
Article Snippet: .. One hundred μl of the Detachment Medium For Primate ES Cells (manufactured by ReproCELL) was added in the ring and cultured at 37° C. for 20 minutes. ..
Article Title: Human pluripotent stem cells induced from undifferentiated stem cells derived from a human postnatal tissue
Article Snippet: .. The medium was removed, washed with the Hank's balanced salt solution, the Detachment Medium For Primate ES Cells (manufactured by ReproCELL) was added, cultured at 37° C. for 10 minutes, and 2 ml of the medium was added to stop the reaction. ..
Article Title: Human pluripotent stem cells induced from undifferentiated stem cells derived from a human postnatal tissue
Article Snippet: .. One hundred μl of the Detachment Medium For Primate ES Cells (manufactured by ReproCELL) was added in the ring and cultured at 37° C. for 10 to 20 minutes. ..
Article Title: Cell culture apparatus and cell culture method using the same
Article Snippet: .. An example of the experiment will be described below. (Cells, Culture Medium, and Reagents Used) Cells: Mouse embryo fibroblasts (ReproCELL Incorporated) and Human iPS cells 201B7 and 253G1 (RIKEN) Culture medium: Medium for primate ES/iPS cells (trade name: Primate ES Cell Medium, ReproCELL Incorporated) Reagents: Recombinant human fibroblast basic growth factor (general name: bFGF, ReproCELL Incorporated), Cell detachment solution for ES/iPS cells (trade name: CTK solution, ReproCELL Incorporated), Phosphate buffered saline (Life Technologies), Cell dissociation solution (trade name: AccuMax, Merk Millipore), Serum-free maintenance medium for human ES/iPS cells (trade name: mTeSR1, STEMCELL TECHNOLOGIES), and Y-27632 (general name for compound, Calbiochem) (Culture Method Using Cell Culture Apparatus) Examples of a method for preparing iPS cells to be cultured in the cell culture apparatus according to the present invention include: (1) inoculation of cell aggregates and (2) inoculation of single cell suspension, and the methods (1) and (2) will be described later. ..
Recombinant:Article Title: Cell culture apparatus and cell culture method using the same
Article Snippet: .. An example of the experiment will be described below. (Cells, Culture Medium, and Reagents Used) Cells: Mouse embryo fibroblasts (ReproCELL Incorporated) and Human iPS cells 201B7 and 253G1 (RIKEN) Culture medium: Medium for primate ES/iPS cells (trade name: Primate ES Cell Medium, ReproCELL Incorporated) Reagents: Recombinant human fibroblast basic growth factor (general name: bFGF, ReproCELL Incorporated), Cell detachment solution for ES/iPS cells (trade name: CTK solution, ReproCELL Incorporated), Phosphate buffered saline (Life Technologies), Cell dissociation solution (trade name: AccuMax, Merk Millipore), Serum-free maintenance medium for human ES/iPS cells (trade name: mTeSR1, STEMCELL TECHNOLOGIES), and Y-27632 (general name for compound, Calbiochem) (Culture Method Using Cell Culture Apparatus) Examples of a method for preparing iPS cells to be cultured in the cell culture apparatus according to the present invention include: (1) inoculation of cell aggregates and (2) inoculation of single cell suspension, and the methods (1) and (2) will be described later. ..
Saline:Article Title: Cell culture apparatus and cell culture method using the same
Article Snippet: .. An example of the experiment will be described below. (Cells, Culture Medium, and Reagents Used) Cells: Mouse embryo fibroblasts (ReproCELL Incorporated) and Human iPS cells 201B7 and 253G1 (RIKEN) Culture medium: Medium for primate ES/iPS cells (trade name: Primate ES Cell Medium, ReproCELL Incorporated) Reagents: Recombinant human fibroblast basic growth factor (general name: bFGF, ReproCELL Incorporated), Cell detachment solution for ES/iPS cells (trade name: CTK solution, ReproCELL Incorporated), Phosphate buffered saline (Life Technologies), Cell dissociation solution (trade name: AccuMax, Merk Millipore), Serum-free maintenance medium for human ES/iPS cells (trade name: mTeSR1, STEMCELL TECHNOLOGIES), and Y-27632 (general name for compound, Calbiochem) (Culture Method Using Cell Culture Apparatus) Examples of a method for preparing iPS cells to be cultured in the cell culture apparatus according to the present invention include: (1) inoculation of cell aggregates and (2) inoculation of single cell suspension, and the methods (1) and (2) will be described later. ..
Suspension:Article Title: Cell culture apparatus and cell culture method using the same
Article Snippet: .. An example of the experiment will be described below. (Cells, Culture Medium, and Reagents Used) Cells: Mouse embryo fibroblasts (ReproCELL Incorporated) and Human iPS cells 201B7 and 253G1 (RIKEN) Culture medium: Medium for primate ES/iPS cells (trade name: Primate ES Cell Medium, ReproCELL Incorporated) Reagents: Recombinant human fibroblast basic growth factor (general name: bFGF, ReproCELL Incorporated), Cell detachment solution for ES/iPS cells (trade name: CTK solution, ReproCELL Incorporated), Phosphate buffered saline (Life Technologies), Cell dissociation solution (trade name: AccuMax, Merk Millipore), Serum-free maintenance medium for human ES/iPS cells (trade name: mTeSR1, STEMCELL TECHNOLOGIES), and Y-27632 (general name for compound, Calbiochem) (Culture Method Using Cell Culture Apparatus) Examples of a method for preparing iPS cells to be cultured in the cell culture apparatus according to the present invention include: (1) inoculation of cell aggregates and (2) inoculation of single cell suspension, and the methods (1) and (2) will be described later. ..
Article Title: Method for inducing differentiation of pluripotent stem cells into cardiomyocytes
Article Snippet: Culture for inducing differentiation in cmES cells was accomplished in a routine manner as follows. cmES cells were washed with PBS and then treated with a commercially available cell dissociation solution for primate ES cells (ReproCELL) at 37° C. for 5 minutes to collect a cell suspension containing cmES cell aggregates. .. Culture for inducing differentiation in cmES cells was accomplished in a routine manner as follows. cmES cells were washed with PBS and then treated with a commercially available cell dissociation solution for primate ES cells (ReproCELL) at 37° C. for 5 minutes to collect a cell suspension containing cmES cell aggregates. .. This non-passed fraction containing cmES cell aggregates was further seeded in a commercially available culture plate with high cell attachment (Primaria; Becton Dickinson) and cultured for 30 minutes, followed by collection of cell aggregates floating in the medium without adhering to the plate.
|