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destaining buffer (30% of ethanol and 10% of acetic solution in h 2 o)  (Bio-Rad)


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    Structured Review

    Bio-Rad destaining buffer (30% of ethanol and 10% of acetic solution in h 2 o)
    Destaining Buffer (30% Of Ethanol And 10% Of Acetic Solution In H 2 O), supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/destaining+buffer/destain+buffer/pmc10262546-116-6-37
    Average 90 stars, based on 1 article reviews
    destaining buffer (30% of ethanol and 10% of acetic solution in h 2 o) - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Effect of aluminium on migration of oestrogen unresponsive MDA-MB-231 human breast cancer cells in culture.
    Article Snippet: Aluminium (Al) has been measured in human breast tissue, and may be a contributory factor in breast cancer development.. At the 10 th Keele meeting, we reported that longterm exposure to Al could increase migratory properties of oestrogen-responsive MCF-7 human breast cancer cells suggesting a role for Al in the metastatic process.. We now report that long-term exposure (20-25 weeks) to Al chloride or Al chlorohydrate at 10 -4 M or 10 -5 M concentrations can also increase the migration of oestrogen unresponsive MDA-MB-231 human breast cancer cells as measured using time-lapse microscopy and xCELLigence technology.

    Article Title: ABCA1 transporter promotes the motility of human melanoma cells by modulating their plasma membrane organization
    Article Snippet: Gels were then stained with the Staining solution (0.5% Coomassie Brilliant Blue R-250 (Merck), 30% of ethanol, and 10% of acetic acid) for 30 min at room temperature with gentle agitation. .. Gels were finally incubated in the Destaining buffer (30% of ethanol and 10% of acetic solution in H 2 O) until clear bands representing the gelatinase activity were visible and pictures were taken with a ChemiDoc System (BioRad). ..

    Article Title: Maternal hypoxia alters matrix metalloproteinase expression patterns and causes cardiac remodeling in fetal and neonatal rats
    Article Snippet: .. The gel was renatured by renaturation buffer (Bio-Rad) for 1 h and then incubated with a development buffer (Bio-Rad) at 37°C for 48 h. The gel was stained with 0.5% Coomassie blue R-250 (Bio-Rad) for 1 h and then destained with destaining buffer (Bio-Rad) until the bands became clear. .. Data were analyzed with the Kodak ID image analysis software.

    Staining:

    Article Title: Effect of aluminium on migration of oestrogen unresponsive MDA-MB-231 human breast cancer cells in culture.
    Article Snippet: Aluminium (Al) has been measured in human breast tissue, and may be a contributory factor in breast cancer development.. At the 10 th Keele meeting, we reported that longterm exposure to Al could increase migratory properties of oestrogen-responsive MCF-7 human breast cancer cells suggesting a role for Al in the metastatic process.. We now report that long-term exposure (20-25 weeks) to Al chloride or Al chlorohydrate at 10 -4 M or 10 -5 M concentrations can also increase the migration of oestrogen unresponsive MDA-MB-231 human breast cancer cells as measured using time-lapse microscopy and xCELLigence technology.

    Article Title: Maternal hypoxia alters matrix metalloproteinase expression patterns and causes cardiac remodeling in fetal and neonatal rats
    Article Snippet: .. The gel was renatured by renaturation buffer (Bio-Rad) for 1 h and then incubated with a development buffer (Bio-Rad) at 37°C for 48 h. The gel was stained with 0.5% Coomassie blue R-250 (Bio-Rad) for 1 h and then destained with destaining buffer (Bio-Rad) until the bands became clear. .. Data were analyzed with the Kodak ID image analysis software.

    Article Title: Identification of B-cell epitopes in the capsid protein of avian hepatitis E virus (avian HEV) that are common to human and swine HEVs or unique to avian HEV.
    Article Snippet: After washing with distilled water, the gels were negatively stained with copper stain buffer (Bio-Rad). .. The corresponding 32 kDa ORF2 protein band was excised and the copper stain was removed using the destain buffer (Bio-Rad). .. The destained gel slices containing ORF2 protein were subjected to electroelution using a commercial apparatus (Bio-Rad).

    Article Title: Improvement of some techno-functional properties of aquafaba by pre-fermentation with Lactobacillus plantarum MA2
    Article Snippet: Recently, modification of macromolecules in food products has gained special interest to increase their utilization during food processing.. In this respect, the aim of this study was to modify chickpea aquafaba by using a pre-fermentation process with Lactobacillus plantarum MA2 for 3, 6 and 9 h and physicochemical and technofunctional of the modified aquafaba samples were investigated.. The results showed that fermentation significantly improved foaming expansion, solubility and stability, with shorter fermentation times resulting in lower stability.

    Activity Assay:

    Article Title: ABCA1 transporter promotes the motility of human melanoma cells by modulating their plasma membrane organization
    Article Snippet: Gels were then stained with the Staining solution (0.5% Coomassie Brilliant Blue R-250 (Merck), 30% of ethanol, and 10% of acetic acid) for 30 min at room temperature with gentle agitation. .. Gels were finally incubated in the Destaining buffer (30% of ethanol and 10% of acetic solution in H 2 O) until clear bands representing the gelatinase activity were visible and pictures were taken with a ChemiDoc System (BioRad). ..

    other:

    Article Title: Absolute difference of hepatobiliary transporter multidrug resistance-associated protein (MRP2/Mrp2) in liver tissues and isolated hepatocytes from rat, dog, monkey, and human.
    Article Snippet: We previously reported that hepatobiliary transporter multidrug resistance-associated protein (MRP2/Mrp2) is considered to be the major cause of the interspecies differences detected by efflux of fluorescent substrates in isolated hepatocytes.. In the present study, the interspecies differences of MRP2/Mrp2 were first evaluated by quantitative real-time polymerase chain reaction and Western blotting.. The mRNA levels were able to distinguish the difference among species with a rank order comparable with the corresponding activities observed, whereas the extents of the differences remained unknown.

    Article Title: Absolute quantification of multidrug resistance-associated protein 2 (MRP2/ABCC2) using liquid chromatography tandem mass spectrometry.
    Article Snippet: Article history: Received 3 March 2008 Available online 27 May 2008 The multidrug resistance-associated protein 2 (MRP2/ABCC2) plays an important role in hepatobiliary efflux of many drugs and drug metabolites and has been reported to account for dramatic interspecies differences in the aspects of pharmacokinetics.. In the present study, an absolute quantif i cation method was developed to quantitatively measure MRP2/ABCC2 using LC-MS/MS for detection of a selective tryptic peptide.. A unique 16-mer tryptic peptide was identif i ed by conducting capillary LC nanospray ESI-Q-TOF analysis of the immunoprecipitation-enriched samples of MRP2/ABCC2 following proteolysis with trypsin.

    Imaging:

    Article Title: Improvement of some techno-functional properties of aquafaba by pre-fermentation with Lactobacillus plantarum MA2
    Article Snippet: Recently, modification of macromolecules in food products has gained special interest to increase their utilization during food processing.. In this respect, the aim of this study was to modify chickpea aquafaba by using a pre-fermentation process with Lactobacillus plantarum MA2 for 3, 6 and 9 h and physicochemical and technofunctional of the modified aquafaba samples were investigated.. The results showed that fermentation significantly improved foaming expansion, solubility and stability, with shorter fermentation times resulting in lower stability.



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