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STEMCELL Technologies Inc lymphoprep density gradient solution
Lymphoprep Density Gradient Solution, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/density+gradient+solution+lymphoprep/lymphoprep/pm36095023-61-8-12
Average 90 stars, based on 1 article reviews
lymphoprep density gradient solution - by Bioz Stars, 2026-10
90/100 stars

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Isolation:

Article Title: Monoallelic mutations in MMD2 cause autosomal dominant aggressive periodontitis.
Article Snippet: Following incubation, samples were immediately analyzed by flow cytometry using the BD FACSVerse (BD Biosciences) and FlowJo (RRID:SCR_008520) software (Tree Star Software). .. Analysis of CD34-positive cells in peripheral blood Mononuclear cells were isolated from peripheral blood using Lymphoprep (cat-no 07801; STEMCELL Technologies), followed by the lysis of RBCs. .. Mononuclear cells were then incubated with FITC Anti-Human CD45 antibody (cat-no 555482; BD Pharmingen), PE Anti-HumanCD34 PE antibody (cat-no 555822; BD Pharmingen), and anti APC Anti-Human CD14 antibody (catno 555399; BD Pharmingen) in PBS with 2% FBS for 15 min at dark.

Article Title: Glutamine-driven metabolic reprogramming promotes CAR-T cell function through mTOR-SREBP2 mediated HMGCS1 upregulation in ovarian cancer
Article Snippet: .. Briefly, Peripheral blood mononuclear cells (PBMCs) from healthy donors (MiaoShun Biotech, China) were isolated by density gradient centrifugation using Lymphoprep (Stemcell Technologies, Canada), followed by negative selection of T cells with the EasySepTM Human T Cell Isolation Kit (Stemcell Technologies, Canada). .. The purified T cells were activated with anti-human CD3/CD28 magnetic beads (Thermo Fisher Scientific, USA) at a 3:1 bead-to-cell ratio and cultured in X-VIVO 15 serum-free hematopoietic cell medium (Lonza, Switzerland) supplemented with 5% fetal bovine serum and 300 IU/mL recombinant human IL-2 (PeproTech, USA).

Article Title: African strains of Zika virus resist ISG-mediated restriction.
Article Snippet: .. Human peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation using Lymphoprep medium (STEMCELL Technologies). .. Cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% FBS and 1% penicillin-streptomycin at 37 °C in a 5% CO2 atmosphere.

Article Title: SIRPγ modulates effector differentiation of human CD8 T Cells under suboptimal TCR stimulation: implications for immune homeostasis and autoimmunity
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats using a density gradient with Lymphoprep (Stemcell Technologies, Vancouver, BC). ..

Article Title: Unveiling the power of plumbagin: revitalizing exhausted T cells to combat tongue cancer
Article Snippet: .. Initially, the peripheral blood was transported at ambient temperature to the dental key laboratory; at the cell ultra-cleaning table, the peripheral blood was diluted 1:1 with phosphate-buffer saline (PBS; Solarbio, Beijing, China) containing 2% FBS and Lymphoprep (Stemcell, Vancouver, Canada) was utilized for isolation of PBMCs in SepMate tube (Stemcell, Vancouver, Canada). ..

Lysis:

Article Title: Monoallelic mutations in MMD2 cause autosomal dominant aggressive periodontitis.
Article Snippet: Following incubation, samples were immediately analyzed by flow cytometry using the BD FACSVerse (BD Biosciences) and FlowJo (RRID:SCR_008520) software (Tree Star Software). .. Analysis of CD34-positive cells in peripheral blood Mononuclear cells were isolated from peripheral blood using Lymphoprep (cat-no 07801; STEMCELL Technologies), followed by the lysis of RBCs. .. Mononuclear cells were then incubated with FITC Anti-Human CD45 antibody (cat-no 555482; BD Pharmingen), PE Anti-HumanCD34 PE antibody (cat-no 555822; BD Pharmingen), and anti APC Anti-Human CD14 antibody (catno 555399; BD Pharmingen) in PBS with 2% FBS for 15 min at dark.

Gradient Centrifugation:

Article Title: Glutamine-driven metabolic reprogramming promotes CAR-T cell function through mTOR-SREBP2 mediated HMGCS1 upregulation in ovarian cancer
Article Snippet: .. Briefly, Peripheral blood mononuclear cells (PBMCs) from healthy donors (MiaoShun Biotech, China) were isolated by density gradient centrifugation using Lymphoprep (Stemcell Technologies, Canada), followed by negative selection of T cells with the EasySepTM Human T Cell Isolation Kit (Stemcell Technologies, Canada). .. The purified T cells were activated with anti-human CD3/CD28 magnetic beads (Thermo Fisher Scientific, USA) at a 3:1 bead-to-cell ratio and cultured in X-VIVO 15 serum-free hematopoietic cell medium (Lonza, Switzerland) supplemented with 5% fetal bovine serum and 300 IU/mL recombinant human IL-2 (PeproTech, USA).

Article Title: African strains of Zika virus resist ISG-mediated restriction.
Article Snippet: .. Human peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation using Lymphoprep medium (STEMCELL Technologies). .. Cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% FBS and 1% penicillin-streptomycin at 37 °C in a 5% CO2 atmosphere.

Article Title: Influenza A virus disruption of dendritic cell-natural killer cell crosstalk impacts activation of naïve helper and cytotoxic T cell subsets
Article Snippet: Immediately following collection, blood was diluted 2-fold in calcium- and magnesium-free phosphate-buffered saline (pH 7.2; Fisher Scientific). .. Diluted blood was then layered over 15 mL of Lymphoprep (STEMCELL Technologies) followed by density gradient centrifugation at 1,200 g for 10 min and harvest of the buffy coat. .. Residual red blood cells were lysed using 5 mL of ACK lysing buffer (Gibco) for 10 min. PBMCs were washed twice with 1× phosphate-buffered saline, pelleted, suspended, and cryopreserved at −80°C in 90% (v/v) fetal bovine serum (R&D Systems) and 10% (v/v) DMSO (Corning).

Selection:

Article Title: Glutamine-driven metabolic reprogramming promotes CAR-T cell function through mTOR-SREBP2 mediated HMGCS1 upregulation in ovarian cancer
Article Snippet: .. Briefly, Peripheral blood mononuclear cells (PBMCs) from healthy donors (MiaoShun Biotech, China) were isolated by density gradient centrifugation using Lymphoprep (Stemcell Technologies, Canada), followed by negative selection of T cells with the EasySepTM Human T Cell Isolation Kit (Stemcell Technologies, Canada). .. The purified T cells were activated with anti-human CD3/CD28 magnetic beads (Thermo Fisher Scientific, USA) at a 3:1 bead-to-cell ratio and cultured in X-VIVO 15 serum-free hematopoietic cell medium (Lonza, Switzerland) supplemented with 5% fetal bovine serum and 300 IU/mL recombinant human IL-2 (PeproTech, USA).

Cell Isolation:

Article Title: Glutamine-driven metabolic reprogramming promotes CAR-T cell function through mTOR-SREBP2 mediated HMGCS1 upregulation in ovarian cancer
Article Snippet: .. Briefly, Peripheral blood mononuclear cells (PBMCs) from healthy donors (MiaoShun Biotech, China) were isolated by density gradient centrifugation using Lymphoprep (Stemcell Technologies, Canada), followed by negative selection of T cells with the EasySepTM Human T Cell Isolation Kit (Stemcell Technologies, Canada). .. The purified T cells were activated with anti-human CD3/CD28 magnetic beads (Thermo Fisher Scientific, USA) at a 3:1 bead-to-cell ratio and cultured in X-VIVO 15 serum-free hematopoietic cell medium (Lonza, Switzerland) supplemented with 5% fetal bovine serum and 300 IU/mL recombinant human IL-2 (PeproTech, USA).

other:

Article Title: Human macrophages secrete both interferon α and interferon β protein during infection with Mycobacterium tuberculosis
Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated from the buffy coats of healthy donors from the Irish Blood Transfusion Services using density-gradient centrifugation over Lymphoprep (StemCell Technologies).

Article Title: Dietary grape pomace ameliorates intestinal damage and oxidative stress by modulating MAPK-Nrf2/ARE pathways in coccidia-challenged broilers ( Gallus gallus )
Article Snippet: The diluted blood was then gently layered onto a 4 mL Lymphoprep TM density gradient medium (1.077g/mL, Stemcell Technologies, Vancouver, Canada) in a 15 mL tube and centrifuged at 800 × g for 20 min with brakes off.

Saline:

Article Title: Unveiling the power of plumbagin: revitalizing exhausted T cells to combat tongue cancer
Article Snippet: .. Initially, the peripheral blood was transported at ambient temperature to the dental key laboratory; at the cell ultra-cleaning table, the peripheral blood was diluted 1:1 with phosphate-buffer saline (PBS; Solarbio, Beijing, China) containing 2% FBS and Lymphoprep (Stemcell, Vancouver, Canada) was utilized for isolation of PBMCs in SepMate tube (Stemcell, Vancouver, Canada). ..



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