density gradient medium (PromoCell)

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PromoCell density gradient medium
Density Gradient Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/density+gradient+separation/Lymphocyte+Separation+Medium+1077/pmc12893248-249-26-33
Average 96 stars, based on 149 article reviews

density gradient medium - by Bioz Stars, 2026-11

96/100 stars

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Purification:

Article Title: Novel bispecific T-cell engagers overcoming acquired EGFR resistance.
Article Snippet: .. PBMCs from buffy coats were purified by Ficoll density gradient centrifugation (Lymphocyte Separation Medium 1077, Promocell, C-44010) and cryopreserved in FCS containing 10% DMSO at −80°C. ..

Gradient Centrifugation:

Article Title: Trifunctional antibody fusion proteins for targeted co-delivery of 4-1BBL with IL-15 or IL-21 to enhance antitumor immune responses
Article Snippet: HEK293-6E cells were cultured in Freestyle F17-Medium (Thermo Fisher Scientific, A1383501) supplemented with 4 mM GlutaMAX-I (Thermo Fisher Scientific, 35050038), 0,1% Kolliphore P188 (Merck, K4894). .. Human peripheral blood mononuclear cells (PBMC) were isolated from buffy coat of healthy donors (Klinikum Stuttgart/Institut für Klinische Transfusionsmedizin und Immungenetik Ulm gemeinnützige GmbH, Germany) by Ficoll density gradient centrifugation (Lymphocyte Separation Medium 1077, Promocell, C-44010) and cultivated in RPMI 1640, 10% FBS. .. Mitomycin C (M0503) was purchased from Merck.

Article Title: Proprotein convertase subtilisin/kexin type 9–CC-motif chemokine ligand 2 interactions link lipoprotein(a) composition to intermediate monocyte inflammation in coronary artery disease
Article Snippet: .. Cells were isolated by density gradient centrifugation using Lymphocyte Separation Medium 1077 (Promocell, Heidelberg, Germany) and SepMate-15 Tubes (Stemcell, Vancouver, Canada). .. After washing, the PBMCs were resuspended in 90% FBS Gold (Seraglob, Schaffhausen, Switzerland) supplemented with 10% dimethyl sulfoxide (DMSO, >99.9% purity, Sigma–Aldrich, St. Louis, USA) and frozen at −80 °C in a CoolCell reduced-scale freezing container (Corning, Corning, USA).

Article Title: Lanostane-type triterpenoids from ethnomedicinal fungi inhibit endothelial cell proliferation.
Article Snippet: Ethnopharmacological relevance: Ethnomedicinal fungi have a long history of use in the treatment of tumors and inflammatory disorders, pathologies often associated with dysregulated angiogenesis and immune-vascular interactions.. However, their effects on endothelial cell functions relevant to angiogenesis and inflammation remain insufficiently characterized.. Aim of the study: The aim of this study was to identify the bioactive compounds from ethnopharmacologically relevant medicinal fungi by screening their ethanolic extracts for their effects on angiogenesisand inflammation-related endothelial cell functions, followed by the identification and targeted isolation of these compounds using molecular networking and bioactivity-guided fractionation.

Article Title: Extracellular vesicles derived from Enterococcus faecalis: inflammatory activation does not require internalization.
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation using lymphocyte separation medium 1077 (cat. no. C-44010, PromoCell) and LeucoSep tubes (cat. no. 227290, Greiner). .. Monocytes were obtained by magnetic cell sorting using anti-CD14 microbeads (cat. no. 130-050-201, Miltenyi).

Isolation:

Saline:

Article Title: Short-interval longitudinal analysis of immune cell dynamics in heavy draft foals after colostrum intake.
Article Snippet: .. The buffy coat 132 was collected, diluted with phosphate-buffered saline (PBS; pH 7.4), and layered over 4.5 mL of 133 Lymphocyte Separation Medium 1077 (PromoCell, Heidelberg, Germany). ..

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