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Irvine Scientific density gradient sperm separation procedure
Density Gradient Sperm Separation Procedure, supplied by Irvine Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/density+gradient+separation/gradient+purification+sperm/pm41921839-224-1-6
Average 86 stars, based on 1 article reviews
density gradient sperm separation procedure - by Bioz Stars, 2026-11
86/100 stars

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Article Title: Engineering sperm-binding IgG antibodies for the development of an effective nonhormonal female contraception
Article Snippet: Semen volume was measured, and the density gradient sperm separation procedure (Irvine Scientific) was used to extract motile sperm from liquefied ejaculates.

Article Title: A versatile capsule-intravaginal ring platform enables rapid and sustained release of monoclonal antibodies for non-hormonal contraception.
Article Snippet: Word Count: 175) Non-hormonal contraceptives that do not require use immediately before or after intercourse would likely meet the reproductive health needs of millions of women.. We previously reported the engineering of MM008, a highly multivalent monoclonal antibody with 10 Fabs against a unique human sperm antigen, which agglutinates and prevents sperm from swimming through cervicovaginal mucus to reach eggs.. Here, we report the development of a versatile capsule-intravaginal ring (IVR) platform that affords precise tuning of mAb release kinetics, enabling both rapid-on and sustained release of MM008 over 30 days into vaginal fluid simulants in vitro, while fully preserving activity and stability.

Purification:

Article Title: Actions of FGF2, LIF, and IGF1 on bovine embryo survival and conceptus elongation following slow-rate freezing
Article Snippet: .. Sperm purification gradient Isolate® (Irvine Scientific, Santa Ana, CA, USA) was acquired ready to use. ..

Article Title: Cytokine supplementation influences bovine embryo transcriptome during the preimplantation period
Article Snippet: .. Sperm purification gradient Isolate® (Irvine Scientific, Santa Ana, CA, USA) was acquired ready to use. .. The cytokine cocktail, FLI, was supplemented as, FGF2 [40 ng/ml, Source: human FGF2 (Made in-house)], LIF [20 ng/ml, Source: human LIF (Millipore, Catalog no. LIF1050)], and IGF1 [20 ng/ml, Source: human IGF1 (Peprotech, Catalog no. CYT-022)], to the culture medium (Yuan et al., 2017; Stoecklein et al., 2021).

Article Title: Cytokine supplementation influences bovine embryo transcriptome during the preimplantation period
Article Snippet: .. Sperm purification gradient Isolate® (Irvine Scientific, USA) was acquired ready to use. .. The cytokine cocktail, FLI, was supplemented as FGF2 (40 ng/mL, source: human FGF2 (made in-house)), LIF (20 ng/mL, source: human LIF (Millipore, USA; Catalog no. LIF1050)), and IGF1 (20 ng/mL, source: human IGF1 (Peprotech, USA; Catalog no. CYT-022)) to the culture medium ( Yuan et al. 2017 , Stoecklein et al. 2021 ).



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